Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,146,771	T→G	13.8%	V172G (GTG→GGG)	pflC →	putative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,146,771	0	T	G	13.8%	194.5 / 2.4	86	V172G (GTG→GGG)	pflC	putative [formate‑C‑acetyltransferase 2]‑activating enzyme, pyruvate formate‑lyase 1‑activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (19/50);  new base G (8/7);  total (29/57)
Fisher's exact test for biased strand distribution p-value = 6.94e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCG  >  NC_000913/4146539‑4147007                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              gggtccgttttccgCCTTGCCCCTCTGGAACGGGAAGTGATGAAAGATGTCATTTTCTTTCGCGCGTCCGGCGGGGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑Tgttgtt                                                                                                                                                                                                                                         <  1:892802/226‑1 (MQ=255)        atatCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑GAACGCCCCAGCCACGAAACAATTACCGCTGGCGAAATTGGTGGAT‑GAAG‑GGTTGTGCGGTTTa                                                                                                                                                                                                                                  >  1:750082/1‑239 (MQ=255)           acggcctgggcgcgttgggaagggAAGTGATGAAAAATGGCATTTTCTTTCGAACGTCGGGGGGGGGCGTACCGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGTAGTTTGCTACCCGTTTTTCACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTaaa                                                                                                                                                                                                                                <  1:498868/217‑1 (MQ=255)             aGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATGTTGCGAT‑GAAG‑GGTTGTTCGGTTTACAAAt                                                                                                                                                                                                                             >  2:170274/1‑239 (MQ=255)                   gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGACGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCCACTGCGGCTGTGGGGTGGGT‑CATGCGCCCTTGAAGATGCCGG‑AGACGCACCAGCCAGCAAGCTCTTACCGCGGGCGGAATTGGAGGAT‑GAGG‑AGTTTTTCTGTTTAAAAAtatagg                                                                                                                                                                                                                        >  1:188164/1‑233 (MQ=255)                   gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATTGGCCATTGAACCTGCCGG‑AGCAGCCCCAGCCAGCCAGCTATTACCGCTGGCGAAATTG‑GCGGC‑GTAGAGGTTGTTCGATTTTAAAATTATGGa                                                                                                                                                                                                                       >  1:208211/1‑239 (MQ=255)                   gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATgg                                                                                                                                                                                                                        >  2:703293/1‑238 (MQ=255)                         ctGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGa                                                                                                                                                                                                                  >  2:560135/1‑238 (MQ=255)                          tgaaaCGGGAAGTTATGAAATGTGACATTTTTTGTCGCACGTCCGGGGGCGGCGCTCCGCTTTCTGGCGGGGAAGTGTGAATGCAGGCGGAGGTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACt                                                                                                                                                                                                                <  1:170274/236‑1 (MQ=255)                          tGGAACGGGAAGTGATGAAAGATGACATTTTCTTGCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACt                                                                                                                                                                                                                <  2:790881/239‑1 (MQ=255)                               cGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgc                                                                                                                                                                                                           <  2:684591/239‑1 (MQ=255)                               cGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgc                                                                                                                                                                                                           >  2:367691/1‑239 (MQ=255)                                gggAAGTGAGGAAAGAGTGCTTTTTCTTTCGCCTGGTCGGGGGCGGCGTCCCGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGAACGCGACTCAGgcg                                                                                                                                                                                                          <  1:11653/239‑1 (MQ=255)                                 ggAAGGGATGAAAGATTCCATTTTCTTTCGCACGTGTGGCGGCGGGGTCCCGTTTGCTGGCGGCGAAGTGTTAAGGCAGGCGGAGTTTGCTACCCGTTTTTTGCAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcg                                                                                                                                                                                                          <  1:486976/238‑1 (MQ=255)                                  gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGAGGCCCCAGCCAGCAAGCTATTACCGCTGGCGAAATTGGGGGGT‑GAAA‑GGGTTTTGGATTTAAAAAATAAGGAGGCGACTCAGgcggg                                                                                                                                                                                                        >  1:366601/1‑237 (MQ=255)                                  gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTTCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACCCGACTCAGgcgag                                                                                                                                                                                                        >  2:420453/1‑237 (MQ=255)                                  gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACCCGACTCAGGcgcg                                                                                                                                                                                                        >  2:621644/1‑239 (MQ=255)                                     ggtatgaAAGATGCCATTTTCTTTCGCACGTCCGGCTGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGATTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCggg                                                                                                                                                                                                      <  2:731875/235‑1 (MQ=255)                                       tgtgaAAGTTGGCGTTTTCTTTCGCACGTCCGGCGGGGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt                                                                                                                                                                                                    <  1:560135/236‑1 (MQ=255)                                       gattaAAGATGACATTTTCTTTCGCACGGCCGGCGGCGGCGTCCCGTTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGa                                                                                                                                                                                                     <  1:388535/237‑1 (MQ=255)                                       gatgaAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt                                                                                                                                                                                                    <  1:136468/238‑1 (MQ=255)                                       gatgaAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATCACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt                                                                                                                                                                                                    <  1:322846/238‑1 (MQ=255)                                                 gACTTTTTTTTGCCCCCGCCCGGCGGGGGCGTACCGCTTGCTGGGGGGGAAGTGTTAAGGCAGGCGGTGTTTGCTCCCCTTTTTTTACAGCGACGCGGGCTGTGGGGT‑TGTCCATGCGCCATTGAACCTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGa                                                                                                                                                                                          <  1:118322/238‑1 (MQ=255)                                                            tcGCGCGTCCGGGGGCGGCGTCCCGCTTTCTGGGGGCGAAGTGTTAATGCAGGCGGAGTTTGCTCCCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAc                                                                                                                                                                              <  1:703293/239‑1 (MQ=255)                                                              ccgcgcccGGGGGCGGGGTACCGTTTTCTGGCGGGGAAGTGTTAATGCGGGGGGAGTTTGCTACCCGTTTTTTACAGCGCCTGCGGCTGTGGGGGTTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAcgc                                                                                                                                                                            <  1:891741/233‑1 (MQ=255)                                                                cctgcgggggggggctgccccgtttCGGGGGGCGAAGTGTTAGTGCAGGCGGAGTTTGCTCCCCGTTTTTTGCAGCGACGGCGGCTGGGGGGTGTGT‑CATGCGCCATTGAAACGGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAcgcg                                                                                                                                                                           <  1:225899/216‑1 (MQ=255)                                                                    ccGGGGGGGGCGTCCAGTTTTCGGGCGGGGAAGTGTTAAGGCAGGGGGAGTTTGGGACCCGTTTTTTACAGCGCCGGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAGACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTg                                                                                                                                                                      <  1:615432/239‑1 (MQ=255)                                                                           cgctgtcctctttttTGGCGGGGAAGTGTTAATGCGGGCGGTTGTTGCCACCCGTTTTTGACAGGGTCTGCGGCTTGGGGGTGTGT‑AATGGGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCCTTTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAAt                                                                                                                                                                <  1:84867/227‑1 (MQ=255)                                                                               gttgtgttttCTGGGGGAGAAGTGTTAATGGCGGGGGAGTTGGCTCCCCGTTTTTTACGGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCAGTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGc                                                                                                                                                            <  1:127141/231‑1 (MQ=255)                                                                                tCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAGGTGGGGAAAATGAACCTGCCACGCGTGCTGGGGAATCTGCGt                                                                                                                                                          >  2:646532/1‑239 (MQ=255)                                                                                      ttttgtgcggggAAGTGTTAATGCAGGGGGGTTTTGCTTCCCGTTTTTGAACGGGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTg                                                                                                                                                    <  1:88767/233‑1 (MQ=255)                                                                                      tttCTGGGGGCGAAGTGTTTATGCAGGGGGGGTTGTCTACCCGTTTTTTACAGCGACTGCGGCTGTGTGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCAGCAGCCAGCAAGCTGTTACCGCTGGCGAAATGGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCGACGCGTGCTGGAGAATCTGCGTTTGCTg                                                                                                                                                    <  1:203720/239‑1 (MQ=255)                                                                                                               ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑GGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGGT‑GACG‑AGTTGTCTGATTTAAAACTTATTGACGCGATCCAGCCGCGGAGCGTGGTGGAAGTGAACCTGTCACGAGTGCCGTCGGATCTGGGTTTGGTGGTGGGTGGGGGGGGCAACGGGAccc                                                                                                                           >  1:669373/1‑239 (MQ=255)                                                                                                               ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAGG‑GGTTGTTCGATTTAAAAATTATGGACGCGCCCCAGGCGCGGGATGGGGTGAAGATTAAACTGCCACGCGTGCTGGGGAATCTGCGTTtggtggggggggggggggccaaagggtgc                                                                                                                            >  1:213749/1‑215 (MQ=255)                                                                                                               ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATc                                                                                                                            >  2:573226/1‑238 (MQ=255)                                                                                                                 cGGAGTTTGCTACCCTTTTTTTACAGCGACTGCGGCTGGGGGGTTGGT‑CATGCGCCATTGAAACTGCCGG‑GGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TTTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATccc                                                                                                                          <  2:852165/238‑1 (MQ=255)                                                                                                                      tttGCTCCCCTTTTTTTCCAGCGCCTGCGGTTGGGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑CGACGCCCCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGt‑t                                                                                                                    <  1:367691/238‑1 (MQ=255)                                                                                                                      tttGCTACCCGTTTTTTAACGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACGGCCGG‑AGACTCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGATTCAGGCGCGGGATGTAGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGt‑t                                                                                                                    <  2:57325/238‑1 (MQ=255)                                                                                                                        tGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTAc                                                                                                                 <  2:695877/239‑1 (MQ=255)                                                                                                                                gTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGGGCTGGAGAATCTGCGTTTGTTGGGGGGTGAGGGCGTCAACGAGAACCC‑CGTGTTACCCCTGAt                                                                                                          >  1:719276/1‑238 (MQ=255)                                                                                                                                          gCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCCGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGGGTTTTCTGGGGAGTGAGGGCGTCAACGGGATCCCGCGT‑TTACCGCGCACCCCTGGGTTc                                                                                                >  1:411548/1‑238 (MQ=255)                                                                                                                                                   gCTGGGGGGTTGGT‑CATGCGCCATTGAAACGGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGc                                                                                       <  2:45214/238‑1 (MQ=255)                                                                                                                                                        ggggggTGT‑CAGGGGCCATTAAAACGGCCGG‑GGACGCCCCAGCCAGCAAGCTATTACCGGTGGCGAAATTGTGGGAT‑GAAT‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGga                                                                                  <  1:591253/238‑1 (MQ=255)                                                                                                                                                           gtgtct‑CATGCGCACTTGAAACTGCCGG‑AGACGCCCCAGCCAGCAAACTATTCCCGCTGGCGAAATTGTGCGAT‑GAAG‑GTTTGTTCTATTTAAAAAGTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTTGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGaa                                                                               <  2:799688/238‑1 (MQ=255)                                                                                                                                                              tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGGGAATCTGAGTTTACTGGTGGGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTGACGTCCGGCCGGGAGaaaag                                                                            >  1:469679/1‑235 (MQ=255)                                                                                                                                                              tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGGGAGTGAGGGCGTCAACGGGATCCCGCGT‑TTACCGCTGATCCCTGGTTTAACCCCCAGCCggggaaaaat                                                                            >  1:325910/1‑231 (MQ=255)                                                                                                                                                              tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCCGGCAAATCTGCGTTTGCTGGGGAGGGGGGGCGCCAACTGGGTCCGGCGT‑TTACCGCCTAACCCTGGTTTTACCCCCGCCCGGGGGaaaaac                                                                           >  1:460611/1‑235 (MQ=255)                                                                                                                                                              gttttgagcgcCGTTTAATCTTGCGG‑AGACGCCCCCACCAGAAAGGTTTTACCGTTGGCGAAATTGTGCTAT‑TAAG‑TTTTTTGCGATTTAAAAATTCTGGACGCTACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGGTCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAatat                                                                            <  1:466477/232‑1 (MQ=255)                                                                                                                                                                  cATGCCCCATTGAAATTGCCGG‑GGGAGGACCGCGCCGCAACATATTACCGGTGGGGAAATTTGGGGAG‑GAAG‑GTTTGTGTGATTTAAAAATTGTGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcag                                                                        <  1:878296/239‑1 (MQ=255)                                                                                                                                                                   cgccgcCATGGAACCTGCCGG‑GGACGCCCCAGACAGCAAGCTTTTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGCCGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc                                                                       <  1:646532/236‑1 (MQ=255)                                                                                                                                                                   aTGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc                                                                       <  2:366601/239‑1 (MQ=255)                                                                                                                                                                    tggcccATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagca                                                                      <  2:719206/235‑1 (MQ=255)                                                                                                                                                                    tGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGGGGCACCGGGATCCCGCGT‑TTACCGCTGCTCCCTGGTTTACCGCCCACCCGGGGGAAAATgcaaag                                                                      >  1:800344/1‑236 (MQ=255)                                                                                                                                                                        ccATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTTGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc                                                                   <  2:404132/238‑1 (MQ=255)                                                                                                                                                                         taTTGAAACTGCCGT‑AGACCGCCCGACAAGCAAGCTGTTACCGGTGGGGAAATTGTGGGAT‑GAAG‑GGTTGTGTGATTTAAAAATTATGGACGCGATTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGTGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc                                                                 <  1:323172/238‑1 (MQ=255)                                                                                                                                                                         cATTGAAACTGCCGG‑ATACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc                                                                 <  2:526671/239‑1 (MQ=255)                                                                                                                                                                         cATTGAAACTGCCGG‑AGACGCACCAGCCAGTAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcg                                                                  <  2:886691/238‑1 (MQ=255)                                                                                                                                                                         aaTTGAAACTGCCGG‑AGACGAACCAAGCAGCAAGATATTACCGGTGGCGAAAGTGGGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGGGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc                                                                 <  2:836698/238‑1 (MQ=255)                                                                                                                                                                           ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGt                                                                                                                                      >  1:139448/1‑169 (MQ=255)                                                                                                                                                                           ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGt                                                                                                                                      <  2:139448/169‑1 (MQ=255)                                                                                                                                                                           ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGCAGCTg                                                               >  2:730580/1‑239 (MQ=255)                                                                                                                                                                            tGAAACCGCCGG‑GGACGACACAGCCAGCAAGCTATTACCGCTGGCTAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg                                                              <  2:669373/239‑1 (MQ=255)                                                                                                                                                                            ggAAAGTGCCGGAAGACACA‑CAGCCAGCAAGGTATTACGGTTGGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTAGGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg                                                              <  1:621644/238‑1 (MQ=255)                                                                                                                                                                             gAAACTGCCGG‑GAACGCACAGGCCAGCAAGCTATTCCGGTTGGTGAAATTGTG‑GATAGAAT‑GGTGGTTCGATTTAAACATTAGGGAGCCGACTCAGGCGCGGGATTGGGTGAAGAGGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGa                                                             <  1:710769/239‑1 (MQ=255)                                                                                                                                                                                   gCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCACCAGGCGCTGGATGt                                                          >  2:161466/1‑236 (MQ=255)                                                                                                                                                                                   gCCGG‑AGACGCACCAGCAAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGATTGATGGTGAGGGAGGGCGTCAACTGGATCCCGCGT‑TTACCGCGGATCTCTGGTTTAACGCTCAGACGGGGGAATAGGCAGCAGGCGCTGGagggacg                                                       >  2:141676/1‑233 (MQ=255)                                                                                                                                                                                             cctccagtcatcatgttgttcccGCTGGCGATTTTTGTCGAT‑GAAT‑TGTTGTTGGATTTAAAAATTATGGAGGCGACTCAGGCGCGGGATGTGGGGAAGATGAACCGGCGACGCGTGCTGGAGAATCTGCGTTTGCGGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCt                                              <  1:420453/218‑1 (MQ=255)                                                                                                                                                                                                  cgccagaAAGCTTTTACCGCTGGGTAAATTGTGGGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAata                                         <  1:573226/238‑1 (MQ=255)                                                                                                                                                                                                     cagaAAGCTATTACCGCTGGCAAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGAAGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTTAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAata                                         <  2:188164/236‑1 (MQ=255)                                                                                                                                                                                                       gcAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGGGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTAATCCCTGGGTTCAAGCTCAGCCGGGGGAATATGCAGCAGGCCCTGGATGtccggttgccgcgaaaaataagg                                    >  1:582394/1‑217 (MQ=255)                                                                                                                                                                                                        catgCTTTTTCCGCGGGCGAAATTGTGGGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGGCTCAGGCGGGGGTTGGGGTGAAGATGAACCTGCCCCGGGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc                                   <  1:821613/236‑1 (MQ=255)                                                                                                                                                                                                         gagttATTTCCGCTGGCGAAATTGGGCGAT‑GAAG‑TGTTGGTCGATTTAAAAATGACGGACGGGACTCCGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc                                   <  2:148134/234‑1 (MQ=255)                                                                                                                                                                                                         aaGCTATTACCGGTGGCGAAATTGGTCGAT‑GAAG‑TGTTGTTCGATTTAAAAAGTATGGCCGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCGCGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAgg                                    <  2:821079/237‑1 (MQ=255)                                                                                                                                                                                                         aaGCTATTACCGCTGGCGAAATTGTTCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc                                   <  2:213749/238‑1 (MQ=255)                                                                                                                                                                                                         aaGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc                                   <  2:325910/238‑1 (MQ=255)                                                                                                                                                                                                           gCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGGGGCTGGATGTACTGATCCCGCTGAATATCAGGCGGa                                >  2:113458/1‑239 (MQ=255)                                                                                                                                                                                                            ttATTTCCGCTGGGGAAATTTGGGGAT‑GAAG‑TGGTGTTCATTTTAAAAATTATGGACGTGACTCAGGCGCGGGATGTGGTAAAGATTAACCGGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGGCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGAt                               <  1:127705/238‑1 (MQ=255)                                                                                                                                                                                                              aTTAGCGGTGGCAAAATTGGTCGAT‑GAAT‑TGTTGGTCGATTTAAAAATTATGGACGGGAATCGGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATcc                             <  2:776447/239‑1 (MQ=255)                                                                                                                                                                                                                     gtGGCGAAATTGTGGGAG‑GAAG‑GGTTGGTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt                      <  2:337368/238‑1 (MQ=255)                                                                                                                                                                                                                     cTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAAAATGCAGCAGCGCCGGGAGGAACTGGACCCCCTGAAAATCAGGCaagacaaacggtt                      >  1:199833/1‑227 (MQ=255)                                                                                                                                                                                                                      tGGCGAAATTGTGCGAT‑GAAG‑TGTCGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt                      >  2:694158/1‑238 (MQ=255)                                                                                                                                                                                                                      tGGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt                      <  2:800344/238‑1 (MQ=255)                                                                                                                                                                                                                       ggCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTa                     >  2:482347/1‑238 (MQ=255)                                                                                                                                                                                                                            aaTTGTGCGAT‑TAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGttt               <  2:460611/239‑1 (MQ=255)                                                                                                                                                                                                                               tgtgGGAG‑GAAT‑TTTTTTTCGATTTAAAAATTATGGGCGCGACTCAGGCGGGGGATGTGGGGAAGATTGACCTGACACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTtcat            <  1:288957/239‑1 (MQ=255)                                                                                                                                                                                                                                          aG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCg  <  2:750082/239‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              GGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCG  >  NC_000913/4146539‑4147007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑