BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,828	T→A	13.2%	L142F (TTA→TTT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,828	0	T	A	13.2%	262.4 / 7.6	117	L142F (TTA→TTT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base T (70/28); new base A (6/12); total (77/40)
Fisher's exact test for biased strand distribution p-value = 2.85e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

CTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACC  >  NC_000913/4241590‑4242055

cTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCt                                                                                                                                                                                                                                      <  2:72443/239‑1 (MQ=255)

       ggtgtaaaacagcaTTAAGAACTGCAGTTCAGGGAGACATTCCGACGCAAACAGATAACGACAACCGTCAGTATGCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACTCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTGCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑aa                                                                                                                                                                                                                              <  1:156981/230‑1 (MQ=255)

       ggTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑ACAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATAACCTGGAGCCAGTTCCGCAAAATACATTGGC‑GAGAGCATTTAGGCAAAAcaa                                                                                                                                                                                                                                >  1:621661/1‑236 (MQ=255)

                   caTTTAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGGTGCCCG‑AAAATAATCCCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGCATATGAGCTGGAGCCATATCCGCAATAAACATTGGC‑GAGAGCATTGAGGCTACACC‑AACCCCAATAcca                                                                                                                                                                                                                   >  2:601170/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑ACAATAATCACAAAC‑TGGATAAAAGA‑GA‑CC‑AGTTTCCCCCGACTATGAACTGGAGCCAGTTCCTCAATATCCATTGAC‑GCGAGCATTGAAGCTAAACC‑AACGCCacaaacg                                                                                                                                                                                                                   >  1:644060/1‑232 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GC‑CC‑AGTTTCCCGCGAATATGAGCTGGGGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTAGGGCTAAACC‑AAAGCCAAAAcccc                                                                                                                                                                                                                  >  1:198486/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCTAGAATATGAGCTGGAGCCAGTTCCGCACATTACATTGGC‑GGGAACATTAAGGCTAAACC‑AAGGCCataaacgc                                                                                                                                                                                                                  >  2:732926/1‑232 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGTC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                                   >  2:418535/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCCATACCGc                                                                                                                                                                                                                  >  2:768521/1‑239 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                                   >  2:758076/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGc                                                                                                                                                                                                                  >  2:838294/1‑239 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGAATTTAGGCTAAACA‑AACGCaaaaa                                                                                                                                                                                                                      >  1:399191/1‑233 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGCC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCg                                                                                                                                                                                                                   >  2:734355/1‑238 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATAAACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAAAAcccc                                                                                                                                                                                                                  >  2:395732/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATAAGCTGGAGCCAGTTCCGCAATATCAATTGGC‑GAGAGAATTGAGGCTAAACC‑AACCCCAATAcccc                                                                                                                                                                                                                  >  1:773705/1‑237 (MQ=255)

                   caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAAAATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAAAGCATTGAGGCTAAACC‑AATGCCAATACCg                                                                                                                                                                                                                   >  2:646461/1‑238 (MQ=255)

                           aCAGCAGTGCAGGAATCCATTCCGAGGCAAACATAAACCCCCAGCCGTCATTTTTCAGCCAGCTGGAATCACCGGAACGGCCAATAAAATAGTTTCCGAAGTAAACTAAAGGTTGCCCA‑AAAATAATCGAAAAC‑GGGTTAAAAGA‑GA‑CC‑ATTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGAAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATg                                                                                                                                                                                                          <  2:582106/239‑1 (MQ=255)

                             agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCAGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAAT‑GA‑CC‑AGTTTCCTGCGAATATGAGCTGGCGCCAGTCTCCCATTAAACCTTGGC‑GAGAGCATTTGGGCATAACA‑AAGTCTCATACCTCCAATAATCCg                                                                                                                                                                                                        >  2:204587/1‑239 (MQ=255)

                             agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGACATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAACTAATCGCAACA‑TGGTTAAAAGA‑GA‑CC‑AGTTTCACCCGAATATAAGCTGAGGCCACTTCCGCAAGATCCATTGGC‑GAGAGCATTAAGACTAAACC‑AACGCCACTACAGCCaacaatccc                                                                                                                                                                                                        >  2:290001/1‑236 (MQ=255)

                               cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCATCCAGATGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAACCTAAAAGTTACACG‑ACAATAATCTCAACA‑CGGCTAAAGGA‑GA‑CC‑AGTTTCCCTCGAGTATCACCTGCAGCCAGTTCCCCCACAAACATTGGC‑TAGAGGATTGAGACAAAACC‑AAACCCAAAAACCCCaaaaaagggga                                                                                                                                                                                                      >  1:427897/1‑233 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGTC‑GAGGGAATTGAGGCAAAACC‑AACGCCAATACCGCCAAAAATGCGaaaaa                                                                                                                                                                                                   >  1:727792/1‑239 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTAGC‑GAGAGCATTAAGGCAAAACC‑AACGCCAAAAACCCCAAAAAACCGaaaaa                                                                                                                                                                                                   >  1:844883/1‑239 (MQ=255)

                                  tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGACGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCACG‑AAAATAATCGCAAAC‑TGCTTAAAAGA‑GA‑CC‑AGATTACCGCGAATATGAGCTGGAGCCAGTTCCGCAATAAACATTGCC‑GAGAGCATTCAGGCTAAAAC‑AACGCCCATACCGACAAAAAAGCGaaaaa                                                                                                                                                                                                   >  1:240882/1‑239 (MQ=255)

                                    cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCCGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAACA‑GA‑CC‑AGTTTCCCGCGCATATGAGCTGGAGCCAGGTCCGCAATATACATTGGC‑GGGAGCATTTAGGCTAAACC‑AACGCCAAAACCCCCaataaaacaacaaaaa                                                                                                                                                                                                 >  1:111927/1‑228 (MQ=255)

                                         aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCACAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCAAAACC‑AACGCCAATACCCCCAAAAAAGCGataaaacaaaaa                                                                                                                                                                                            >  1:72155/1‑232 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATCCATTGGC‑GAGCGCATTGAAGCTAAACC‑CACGCCAATACCCCCAAAAATGCGTTaaaaaaaaaaat                                                                                                                                                                                          >  1:896413/1‑236 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                                                                          >  2:662029/1‑238 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGCC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAAtt                                                                                                                                                                                          >  2:798694/1‑238 (MQ=255)

                                            cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCCCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AAAGCCAATACCGCCAAAAAAGCGATAAAAAAAAAAATc                                                                                                                                                                                         >  1:258426/1‑239 (MQ=255)

                                             ttcccGAGGAAAACATAAACCCCCAGCCGTCAGTATTCAGCAAGCGGGAAACACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTGGCCCG‑AAAATAATCGAAACC‑TGGTTAAAAGA‑GA‑CC‑AGTTCCCCGCAAATATGAGCTGGAGCCAGTCCCGCAATATACATTGGA‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                        <  2:315261/236‑1 (MQ=255)

                                             aTTCCGAGTAAAACATAAAACGCCAGCCGACAGTATTCAGCCAGCTGCAATCACCGGAACGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTAGCCCG‑AAAAAAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑ATTTCCCCGCAAATAGGAGCTGGAGCCAGTCCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTcc                                                                                                                                                                                        <  2:152723/239‑1 (MQ=255)

                                              ttCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCCCCAATAATGCGATAAATAACAAATTCCg                                                                                                                                                                                       >  2:442265/1‑239 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGCATATGAGCTGGAGCCAGTTACGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AAAGCCAATACCCCCAATAATTCGTAAAATAAAAAATTccagaaac                                                                                                                                                                                  >  1:890860/1‑233 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCAGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑ACCGCCAATACCGCCAATAATTCGATAACTACCAAATTCCGGGACa                                                                                                                                                                                  >  2:827301/1‑239 (MQ=255)

                                                   aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATAAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAACATTGAGGCTAAACC‑AACGCCAAAACCGCCAAAAAAGGCAAAAAAAAAAAAATCCGGGaaa                                                                                                                                                                                  >  1:753456/1‑237 (MQ=255)

                                                    ggCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACAt                                                                                                                                                                                 >  2:447886/1‑239 (MQ=255)

                                                               aggcccACCCGTCAGTTACCAGCCAGCTGGAATCACCGGAACGGGCAAAAAAATAGTTTACCCAATAAACTAAAAGTTGCCCG‑AAAATAATCCAAAAT‑GGGTAAAAAGA‑GA‑CC‑AGTTCCCCGCGAATAGGAGCTGGACCCAGTACCGCAATATACATGGGC‑GAGAGCATTGAGGAAAAACA‑ACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                      <  2:390972/235‑1 (MQ=255)

                                                               aaCGCCGGCCGTCATTTTTCAGCCAGGTGGCAACCCCGGACCGGGAAAAAAAATAGTTTACGAAGTAACCTAAAATTTCCCCG‑AAAATAATCGAAAAC‑TGGTTAAAAGA‑GA‑CC‑GGTTCCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                      <  2:32046/239‑1 (MQ=255)

                                                               aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGTC‑GAGAGCATTGAGCCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATACCAAAATCCGGGACAAAACCCGCCAGa                                                                                                                                                                      >  1:692242/1‑239 (MQ=255)

                                                               aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGGGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTACGGGACATAACCTGCCAGa                                                                                                                                                                      >  2:217729/1‑239 (MQ=255)

                                                                     aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACa                                                                                                                                                                >  2:677796/1‑239 (MQ=255)

                                                                     aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCAGGGACATAACCTGCCAGATAAACa                                                                                                                                                                >  2:582628/1‑239 (MQ=255)

                                                                      gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAAAATACATTGGC‑GAGAGCATTGAGGCCAAACC‑AACGCCAATACCCCCAATAATGCGAAAAAAAACAAATTCCCGGACATAACCTGCCAAAGaaaacg                                                                                                                                                               >  1:242988/1‑236 (MQ=255)

                                                                       ccGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAAAAAATTCCGGAAAAAAACAGGCCAAAAAAAAAgg                                                                                                                                                              >  1:431892/1‑239 (MQ=255)

                                                                        cGTCAGTATTCAGCCAGCGGGCATACCGGGAACGGGAAATAAAATAGTTTCCGAAGTAAACAAAAAGTTGCCAA‑AAAAAAATCGCAAAC‑GGGTTAAAAGA‑GA‑CC‑ATTTTCCGGGGAATATGAGCTGGAGCCAGTTCCGCAATATACATGGGA‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGc                                                                                                                                                             <  2:538632/239‑1 (MQ=255)

                                                                         tttttttttCAGCCAGCTGGCAACCCCGGAACGGAAAATAAAATAGTTTACGAAGTAAACTAAAATTTCCCGA‑AAAATAACCGAAAAG‑TGGTAAAAAGA‑AC‑CC‑AGTCTCCCGCGAATATGAGCGGGACCCAGTTCCGCAATATACATGGGC‑GAGAGCATTGAGGCTAACCC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGca                                                                                                                                                            <  1:209374/232‑1 (MQ=255)

                                                                            attATCCAGACAGCTGGCATCCGCGGAGCGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCA‑AAAAGAATGGCAAGC‑TGGTTAAAAGA‑GA‑CC‑GGTTTCCGGGGAATATGAGCTGGAGCCAGTTCCGCAATATCCATTGGG‑GAGAGCATTGAGGATAAACC‑AGCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                                                                                                                         <  2:77381/237‑1 (MQ=255)

                                                                                   agccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCCCAGagttgt                                                                                                                                                   >  2:305764/1‑238 (MQ=255)

                                                                                      cagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGAAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTCCCCGCGAATATGAGCTGGAGCCAGTCCCGAAATATACATTGGC‑GAGAGCATGGAGGCTAACCC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                                                                               <  2:477527/239‑1 (MQ=255)

                                                                                             cATCACAGGAAAGGGCAAAAAAATTGTTTACGCAGTAAACAAAATGTAGCCCG‑AAAATAACCGCAAAC‑GGGTAAAAAGA‑GA‑CA‑AGTTACCCGCGAATAGGAGCTGGAGCCAGTCCCGCAATATACATTGGC‑GAGAGCATTGAGGCAAAACC‑AACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtat                                                                                                                                         <  2:890860/238‑1 (MQ=255)

                                                                                              acccccgaagcggtaaaTAAAATTTTTTACGCAGTAAACTAAAATGTGCGAG‑AAAAAAAGCGAAACG‑TGGTTAAAAGA‑GA‑CC‑AGTTCCCCGGAAATATGAGCTGGAGCCAGTCCCACAATATACTTGGGC‑GAGAGCATTGAGTCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtata                                                                                                                                        <  1:416888/223‑1 (MQ=255)

                                                                                                   cGGAACGGTCAATAAAAAAGTTAACGAATTAAATAAAAAGTTGCCGA‑AAAATAA‑CGCAAACTTGGTAAAAGGA‑GA‑CC‑AGTTCCCCGGGAAAAAGAGCGGGAGCAAGTTCCGAAATATACTTGGGC‑GAGAGCATTGAGGTTAAACC‑ATCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                                  <  2:242988/239‑1 (MQ=255)

                                                                                                   cGGAACGGGCAATAAAATGGTTTAGGAAGAAAACTAAAAGTGGCCGA‑AAAATAAACGAAAAC‑TGGTTAAAAGG‑AA‑CC‑AGTGCCCGGCGAATATGAGCTGGAGCCAGTCCCGCAATATACATGGGC‑GAGAGCATTGAGGCAAACCC‑AACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                                  <  2:896413/239‑1 (MQ=255)

                                                                                                          ggCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAAAGGAACAGTGTTTTCCGGGGTTAAAAAGGACAAACCaa                                                                                                                           >  1:183910/1‑239 (MQ=255)

                                                                                                                 aagtatgttACACAGAAAACGAAAAGTCGCACG‑AAAATAAGCGCAAAT‑TGTTAAAAAGA‑GA‑CC‑ATTTTCGCGCGAATAGGAGGGCGAACCAGTTCCCAAATAGACATGGGG‑GAGAGAAATGAGGTAAAACC‑AACGAAAAGACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTgg                                                                                                                    <  2:303834/232‑1 (MQ=255)

                                                                                                                        ttACGCAAAAAAAAAAAAGTTCCCAG‑AAAATAAGCGAAAAG‑GTGTAAAAAGA‑CA‑CC‑ATGTTCCCGCGAATATGAGCTGGACCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGATAAACC‑AACGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                             <  1:645694/239‑1 (MQ=255)

                                                                                                                         tACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAg                                                                                                            >  2:195718/1‑239 (MQ=255)

                                                                                                                         atccggaaaaaaataaaGGTGCCCAAAAAAAAACCGCAAAC‑TGGTTAAAAGA‑AA‑CC‑AGTTCCCCGGGAATATGGGCTGGAGCCAGTTCCGAAATATACATTGGG‑GAGAGCATTGAGGCTAACCC‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                             <  2:727792/225‑1 (MQ=255)

                                                                                                                            gCAGTAAACTAAAAGTTGCCAA‑AAAATAATCGAAAAC‑GGGTTAAAAGA‑GA‑CC‑AGTGTCCCGCGAATATGAGCTGGAGCCAGTTCCACAATATACATTGGC‑GAGAGAATTGAGGCTAAACC‑ACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAAc                                                                                                         <  2:844883/239‑1 (MQ=255)

                                                                                                                              aGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGTTTAAAAGA‑GA‑CA‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGCCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATCACAAATTCCGGGGCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGCTCTGGCCCGGCAAAACCt                                                                                                       >  2:802011/1‑239 (MQ=255)

                                                                                                                                tAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGCCATAACCTGCCAGATAACCAGGCCCCGTGTTGTCCGGGTTTATTAAGGCAAAACCAAGTTCTGGCCAgcacgaaccca                                                                                                      >  1:520829/1‑228 (MQ=255)

                                                                                                                                tAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGAAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTACAGGCAAGGCAAAAccaaa                                                                                                     >  1:325460/1‑236 (MQ=255)

                                                                                                                                           tttgccagaaataacccGCAAGC‑TGGTTAAAAG‑‑GA‑CCTAGTTCCCCGGGAATATGAGCTGGAGCCATTCCAGAAATAAACTGGGGC‑GAGAGCATGGAGGCTAAACA‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                                                                           <  2:198486/223‑1 (MQ=255)

                                                                                                                                             tcaccaaaaaaaATCCC‑AAC‑TGGTTAAAAAAGGA‑CC‑AGTTCCCCGAAAATAGGGCCGGGAGCCATTCCCGAAATAACCATGGGC‑GAGAGCATAGAGGCTAACCC‑AACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                                                                           <  1:85585/231‑1 (MQ=255)

                                                                                                                                              cccG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGGGCCAGTTCCGCAATATACATTGGC‑GAGCGCATTGAGGCTAAACC‑AACGCCCATACCGCCAATAATGCGATAAATAACACATTCCGGGCCATAACCTGCCAGATAAACAGGCACAGTGTTGTCCAGGTTTATAGAGGTAAAACCAAATGCCGACCAGGCCGAACCTCCCCCATAaaaaaaaa                                                                                       >  1:774238/1‑239 (MQ=255)

                                                                                                                                                      aatatccccaaC‑TGTTTAAAAGA‑CC‑CT‑GGTTCCCCGGAAATGGGAGCTGGAACCATTTCCGAAATATAAATTGGC‑GAGAACTTTGGGGATAAACA‑AAAGTCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAc                                                                                <  1:696981/230‑1 (MQ=255)

                                                                                                                                                       aaaacctaaaattgtgaaaaaaa‑GACCC‑AGTTCCCCGCAAATAGGAG‑GGGGGCCCGGTCCCGAAAAAAAATTGGCGGAGAGCATTGA‑GCAAAACCAAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                                                 <  1:662029/222‑1 (MQ=255)

                                                                                                                                                           ccaaaaC‑TGGTAAAAAAG‑GC‑CC‑ATTTCCCCGAGAAAAGGGGCTGGAGCCAGTTCCGAAATATACATTGGG‑GAAAGCATTGAGGCAAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                                           <  2:431892/236‑1 (MQ=255)

                                                                                                                                                              caacgtGGTAAAAAGA‑GA‑CC‑AGTTCCCCGAGAATAGGAGCTGGACCCAGTCCCGAAATATAAATGGGC‑GAGAGCATTGAGCCAAAACC‑ACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAgcagca                                                                         <  2:621661/234‑1 (MQ=255)

                                                                                                                                                                actgggTTACAAGA‑CA‑CC‑GTTTTCCCGGGAATTTGGGCGTGAGCGAGTCCCCCAATAT‑CCTTGGCAGAGAGATGCGAGGATAAACA‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAGCCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACAACAGAAATAAAAAACAGGACAGCAGCAAt                                                                       <  1:571007/235‑1 (MQ=255)

                                                                                                                                                                      ttAAAAGG‑AC‑CC‑AGTTCCCCGAGAATATGAGCGGGACCCAGTCCCGCAATATACGTTGGA‑GAGAGCATTGAGGCTAACCC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAg                                                                 <  2:852040/239‑1 (MQ=255)

                                                                                                                                                                      taaaaaaa‑aa‑CC‑AGTTCCCGGGAAATGTGGGCGGGAGCAATCTCCCAAATATCAATGGGA‑GAGAGCATTGAGGCAAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                  <  1:447886/236‑1 (MQ=255)

                                                                                                                                                                        taaaaaggaccc‑AGTTCCCCGGAAATTGGAGCGTGAGCCAGTTCCGCAATATACATTGGC‑GGGAGCATTGAGTCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                  <  2:833364/228‑1 (MQ=255)

                                                                                                                                                                                a‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                                        >  2:386308/1‑239 (MQ=255)

                                                                                                                                                                                a‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGTTTATAGAGGTAAAACCCAGATCTGGCCAAGCAGAACCTACACCAGAAATAAAAAAAAGGAAAGCAGCaaattaaaagaaaaaaag                                                        >  1:811982/1‑223 (MQ=255)

                                                                                                                                                                                       tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCAcgacga                                                    >  2:104182/1‑238 (MQ=255)

                                                                                                                                                                                       tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCAcgacga                                                    >  2:683779/1‑238 (MQ=255)

                                                                                                                                                                                       tttCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTAACCCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTaaaaaacaagaa                                                    >  1:326492/1‑229 (MQ=255)

                                                                                                                                                                                          cccGGGAATATGACCGGGACCCAGTCCCACAATATACATTGGC‑GAGAGCATGGAGGCTAAACC‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGa                                                <  2:192809/239‑1 (MQ=255)

                                                                                                                                                                                                  tatGAGTGGGAGCCAGTCCCGAAATAGCATTTGGC‑GAAGGCATTGAGGCTAACCC‑ACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                                        <  2:509040/239‑1 (MQ=255)

                                                                                                                                                                                                   atGAGCTGGAGCCAGGTCCGCAATATCCATTGGC‑GAGGGCATGGAGGCTAAACC‑AACGCAAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTAc                                       <  2:72155/239‑1 (MQ=255)

                                                                                                                                                                                                       gtgggAGCCAGTTCCACAATATACATTGGC‑GAGAGCATTGAGCAAAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGc                                    <  2:258426/235‑1 (MQ=255)

                                                                                                                                                                                                         ggggcccAGTCCCGAAATATACTTGGGC‑AAGAGATTTAAGGAAAACCA‑AATGCCAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAAt                                 <  1:646461/234‑1 (MQ=255)

                                                                                                                                                                                                           gAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                >  2:859003/1‑238 (MQ=255)

                                                                                                                                                                                                            aGCCAGTTCCTAAATATACAGTGGC‑GAGAGCATTGAGGCTAAACA‑AACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATaa                               <  2:520829/238‑1 (MQ=255)

                                                                                                                                                                                                             gCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAACCGGTTCCTGCAATAacc                             >  2:171876/1‑239 (MQ=255)

                                                                                                                                                                                                                               aTTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCAcccccggt           >  2:374639/1‑237 (MQ=255)

                                                                                                                                                                                                                               aTTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCgat           >  2:23256/1‑239 (MQ=255)

                                                                                                                                                                                                                                 tGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga         >  2:24338/1‑239 (MQ=255)

                                                                                                                                                                                                                                 tGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGACCCGCCgatga         >  2:771725/1‑239 (MQ=255)

                                                                                                                                                                                                                                 tGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAACGGGTTACTCCAAAAACCAACCAGGGCaccacccaaga         >  1:207445/1‑234 (MQ=255)

                                                                                                                                                                                                                                    c‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCCAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc      >  2:394927/1‑239 (MQ=255)

                                                                                                                                                                                                                                    c‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAACGGTTACTGCAATAACAACCGAGGGCACCCCCgatgat        >  2:388584/1‑237 (MQ=255)

                                                                                                                                                                                                                                    c‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCCACACCAGAAACAAAAAACCGAACAGCAGCAATCTTACGCGAATCACGACGACCGAAGCGCTACCTGCAAAAAAAACCCAGGGAACAGCCgataaaca      >  1:363670/1‑234 (MQ=255)

                                                                                                                                                                                                                                       agagCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGTGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGAAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATa                                >  2:867945/1‑211 (MQ=255)

                                                                                                                                                                                                                                       agagCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAAGCGGTTACTGCAATAACCACCCAGGGCACCGCCGAATATGCa     >  2:779998/1‑238 (MQ=255)

                                                                                                                                                                                                                                       agagCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGAGGATGCa     >  2:30926/1‑238 (MQ=255)

                                                                                                                                                                                                                                       agagCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGACCCCCCGATGATGaaa    >  1:775138/1‑239 (MQ=255)

                                                                                                                                                                                                                                       agagCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGGTACTGCAATAACACCCGAGGGAAcgcccaaggaggcaa    >  1:671673/1‑225 (MQ=255)

                                                                                                                                                                                                                                         agCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACACCGACCAAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGTGTATGCAAcc  >  2:706940/1‑239 (MQ=255)

CTGGCACGGTATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCG‑AAAATAATCGCAAAC‑TGGTTAAAAGA‑GA‑CC‑AGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGC‑GAGAGCATTGAGGCTAAACC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACC  >  NC_000913/4241590‑4242055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`