Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,289,530 T→G 12.7% V96G (GTG→GGG)  nrfB → nitrite reductase, formate‑dependent, penta‑heme cytochrome c

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,289,5300TG12.7% 242.1 / 2.5 106V96G (GTG→GGG) nrfBnitrite reductase, formate‑dependent, penta‑heme cytochrome c
Reads supporting (aligned to +/- strand):  ref base T (45/41);  new base G (10/7);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTGTGGAGCCTGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAA  >  NC_000913/4289295‑4289767
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
ttttgGGGCCTGAACGGAATTGCCGCTACCCCTTCGGCGCAGGGATCGGATGATCGCTACGAAGTTTCCCAGCAGGGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑At                                                                                                                                                                                                                                             <  1:785039/236‑1 (MQ=255)
ttgtGGAGCCTGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAACCATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGAAGTG‑At                                                                                                                                                                                                                                             >  2:378476/1‑239 (MQ=255)
 tggggAGCCCTAACGGAATTACCGCCACGCCTTCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCGGAATAACTAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑At                                                                                                                                                                                                                                             <  2:361646/235‑1 (MQ=255)
 tggcgacgcTGAACGGAATTACCGCTACCCCGGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑At                                                                                                                                                                                                                                             <  2:807452/230‑1 (MQ=255)
     gAGCCTGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGCAGGGGTGAAAGATGTG‑ATGCGCt                                                                                                                                                                                                                                        >  2:460723/1‑239 (MQ=255)
          tGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGCAGGGGTGAAAGATGGG‑ATGCGCTTTAAc                                                                                                                                                                                                                                   >  2:395330/1‑239 (MQ=255)
          tGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGCAGGGGGGAAAGATGTG‑AGGCGCTTTAAc                                                                                                                                                                                                                                   >  2:176114/1‑239 (MQ=255)
          tGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACAAACTGT‑CACGGCCAGCAATCAACCAAACACCGCCAAGGGGGAAAAAAGGAG‑ATGACCTTTaa                                                                                                                                                                                                                                    >  1:361646/1‑238 (MQ=255)
             aCGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTAACCTGCCCCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGGGAAAGATGGG‑ATGCGCTTTAAAGa                                                                                                                                                                                                                                 >  2:421596/1‑238 (MQ=255)
              cGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGc                                                                                                                                                                                                                               <  2:183123/239‑1 (MQ=255)
                     aCCGCGACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTCCCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATTCCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCATCCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTa                                                                                                                                                                                                                        <  2:628288/239‑1 (MQ=255)
                      ccGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGCGAAAAATGAG‑GAGCGCCTTAAAGAGcaggagtac                                                                                                                                                                                                                       >  1:412772/1‑231 (MQ=255)
                           aCCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCCTG‑GAAAACATGCTTCCGTTATCCACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACCGT‑CACGGCCAGCCATACCCCCAACACCGCGACGGGGGGAAAGATGGG‑ATACGCTTTAACAAACCAAGGGACAAGGt                                                                                                                                                                                                                  >  1:316816/1‑239 (MQ=255)
                           aCCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACCCCCCGAAGGGGGGAAAGAGGGG‑AGGCCCTTTAACAAGCCAAGGGACAAAGt                                                                                                                                                                                                                  >  1:55924/1‑239 (MQ=255)
                           aCCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGt                                                                                                                                                                                                                  >  2:518190/1‑239 (MQ=255)
                                 gcggcgCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GGAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTCCACCACCCGT‑CACGGCCAGCCATCAACGCAACACCCCGAAGCGGTGAAAGAAGGG‑ATGGGCTTTACCGAGCCGACGTAACAGGt‑ttggga                                                                                                                                                                                                           >  1:158898/1‑234 (MQ=255)
                                    gcgcAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAAAGCa                                                                                                                                                                                                        >  2:342922/1‑239 (MQ=255)
                                        aGGCATTTGATGAGCGCTACGAAGTTCCCCAGCAGCGTAACCCGGATGCCGCTTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGaa                                                                                                                                                                                                     <  2:724562/238‑1 (MQ=255)
                                        aGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAACCATCACCGCAGCACCGCGAAGGGGTGAAAGATGGG‑ATGGGCTTTAACGAGCCGATGAAAAAGGT‑TGGAGGGCaaaaa                                                                                                                                                                                                    >  1:879580/1‑238 (MQ=255)
                                         ggaatttgatgaCCGCTAGGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCGGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAGCACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACa                                                                                                                                                                                                   <  2:879580/233‑1 (MQ=255)
                                          gCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGGCCAAGGT‑TGGAGAGcgaaaaag                                                                                                                                                                                                  >  2:422649/1‑232 (MQ=255)
                                            cttcgagtacccctccaaaGTTCCCCAGGGGCGTCACCGGGTTGCCGCCTGTATGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑AAAAACATGCTTCCGTTACCACCCCGAATAACAAA‑CTGCCGGCACCCTGCACCAACTGT‑CCCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGc                                                                                                                                                                                                 <  1:878248/221‑1 (MQ=255)
                                            atttgatttcggCTAGGAAGTTCCCCCGCGGGGTAACCCGGTGTCCGCTTGTCTGGACTGTCATAAACCAGATCCCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCg                                                                                                                                                                                                <  2:109137/228‑1 (MQ=255)
                                            atatgtagatCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGc                                                                                                                                                                                                 <  2:633319/231‑1 (MQ=255)
                                            aTCTGATGATCGCTACGAAGTTCCCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCg                                                                                                                                                                                                <  2:361655/239‑1 (MQ=255)
                                                    atCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCCTAAACCAGATACCGAAGGTATGCATG‑GAACACATGCTTCCGTTATCAACCCGACTAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATACACGCCCCCCCGCGACGGGCGCGAAGCTGTG‑ATGCGCTTTACCCGCCCGCTGTACAAGGC‑TGGAGAGACGAAACGCGCCTGTAt                                                                                                                                                                                         >  1:684473/1‑238 (MQ=255)
                                                    atCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATCGGCTTTAACGAGCCGATGTAAAAGGT‑Tgagaagcaaaaaagggccggaagg                                                                                                                                                                                        >  1:205413/1‑216 (MQ=255)
                                                      cGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCACCCATCACCGCACCACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTCAAAGGT‑TGGAGAAAAGAACACCGTCTGAATGt                                                                                                                                                                                       >  1:281840/1‑238 (MQ=255)
                                                        cTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAACCATCACCGCAACACCGCGAAGGGGTGAAAGATGGG‑ATGCGGTTTAACGAGCCGCAGTACAAGGT‑TGGAGAGCAGAAAAGAGTCCGTATGTccg                                                                                                                                                                                    >  1:683062/1‑238 (MQ=255)
                                                            gAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCa                                                                                                                                                                                <  2:586563/239‑1 (MQ=255)
                                                                ttACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGCCTGTCATAAACCAGATACCGAATGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CCCGGCCACCCATCCCCGCCAAACCGCGACGGGGTGCAAGTGGTG‑ATGCGCTTTAACGAGCCCATGT‑CAAGGTGTGGCGAGAGAAAAAGCGGCTGTATATcacgtcctcc                                                                                                                                                                             >  1:142448/1‑229 (MQ=255)
                                                                 tACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCCAACCCGCGAAGGGGTGAAAGATGGG‑ATGCGCTTTAAAGAGCCGATGTACAGGGT‑TGGGGAACAGAAAAGCGTCTGGATGTCCTGTAATATg                                                                                                                                                                            >  1:7057/1‑238 (MQ=255)
                                                                        cagcGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGCTGTCAAAGGT‑TGGGGAGCAGAACAGAGCCTGTATGTCCTGTACTCTGCCGGAACa                                                                                                                                                                    >  2:268971/1‑239 (MQ=255)
                                                                        cagcGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTTGCATCTGCCGGACCa                                                                                                                                                                    >  2:689989/1‑239 (MQ=255)
                                                                          gcGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTCCAAGGT‑TGGAGAGCAGAACAGCGTCTGAAGGTCCTGTCATCTCCCGGAAAAGt                                                                                                                                                                  >  2:428288/1‑239 (MQ=255)
                                                                          gcGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGCG‑ATGCGCCTTAACTAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGt                                                                                                                                                                  >  2:407975/1‑239 (MQ=255)
                                                                                     aTGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGGG‑ATGCGCTTTAACGAGCCGATGGACAAGGA‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTCCCGGAACAGTTGAAAAAAGct                                                                                                                                                       >  2:887882/1‑238 (MQ=255)
                                                                                                ttgggctcaaaaaacccAGAGACCGAAGGT‑TACCTGTGAAAACATCCTTCCTTTACCAACCCGAATAACAAA‑CCGCCGGTCACCATCAACAACGGT‑CACGGCCAGCCATCACCTCAACACCGCGAAGGGGTGAAAGATGTG‑AGGCTCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCa                                                                                                                                            <  1:835040/224‑1 (MQ=255)
                                                                                                cTGTACTGCAATAAACCAGATCCCGAAGGTATGCATG‑GAAAACATGCTTCCGTTCTCAACCCGAATAAAAAA‑CTGCCGGTCACCTGCCCCAACGTT‑CCCGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCa                                                                                                                                            <  2:847410/239‑1 (MQ=255)
                                                                                                  ggACTGTCATAAACCAGATCCCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCCCCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACg                                                                                                                                          <  2:501714/239‑1 (MQ=255)
                                                                                                     tctgctaaaaCCAGTTACAGAGGGTCTGTCTG‑GAAAACTGTCTTCCGTTTACACCCCCAAAAAAAAG‑CGCCTGGCCTCCTGCCCAAACTGA‑CACGGCCAGCTCTCCCCGCAACACCGCAAAGGGTTGAAAGATGGT‑ATGCGCTTTAACGAGTCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATg                                                                                                                                       <  1:118530/232‑1 (MQ=255)
                                                                                                        tCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCTGCACGATGTc                                                                                                                                     >  2:725468/1‑238 (MQ=255)
                                                                                                         cATAAACCAAATACAGAGAGTTGGAATG‑CAAAACATCCTCCCGATATCACCCCGAATGACAAA‑CGGCCGTGCACCTTCCCCAAGTGT‑CACGGCAGACCAGCACCGAAACTAGTCTACGGGGGAAAAGATGGG‑ATGGTCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCTTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTTGCCGCACGATGTCCa                                                                                                                                   <  1:576898/239‑1 (MQ=255)
                                                                                                            aacccATATCCCCAAGGTTTGCTTG‑GAAACCTTTCTTCGTTTATAAACCCGAAAAACAAA‑CTGCCGGTCACCTGCCCCAACTGT‑CCGGGCCAGCCATCCCCGCAACTCCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGt                                                                                                                                <  1:422649/236‑1 (MQ=255)
                                                                                                              aCCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGGGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGCCTGTATGTCCTGTCATCTGCCGGAACAGGTGCCAAAACCGTTCTGCCCGCCCGAGGTCCCCgaa                                                                                                                               >  1:8761/1‑236 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑TGCCCCTTTACCGGGCGGATGTACAAGGT‑TGGAGAGCACAACAGCGTCAGATTGTCCATTCATCTGCAGAAAAAGTAGACAAAGGCGTTACGCCCGAACAAGGACCACGAAACaaa                                                                                                                          >  1:268561/1‑239 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTTTATGTCCTGTCATCTGCCGGACACGTTGCAAAAAGCGTTCTGGCCCAACGATGTCCACGTAACCaa                                                                                                                          >  2:830524/1‑239 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCaaaaa                                                                                                                                                          >  1:10460/1‑207 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCaaaaa                                                                                                                                                          <  2:10460/207‑1 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAACGGTCTGGCCCCCCGATGTCCCCGTAACCaa                                                                                                                          >  2:344271/1‑239 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTACCGGACCAGTTGCAAAAAACGTTCGGGCCGACCGATGTCCACTAAACCaa                                                                                                                          >  2:366063/1‑239 (MQ=255)
                                                                                                                  gATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAAAAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGAGTTCTGGCCGCACGATTTCCACGAAACCaa                                                                                                                          >  1:180296/1‑239 (MQ=255)
                                                                                                                   aTACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCaaa                                                                                                                         >  2:221830/1‑239 (MQ=255)
                                                                                                                          aGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAACAAGCGTTCTGGCCGCCCGATGTCCACGTAACCCAAGTGGCGt                                                                                                                  >  2:844787/1‑239 (MQ=255)
                                                                                                                          aGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGt                                                                                                                  >  2:634480/1‑239 (MQ=255)
                                                                                                                               tGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGcc                                                                                                             >  2:170381/1‑239 (MQ=255)
                                                                                                                               tGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGCCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGcc                                                                                                             >  2:267283/1‑239 (MQ=255)
                                                                                                                                tggtgggaaaaactcctccgcttAACACCCCGAAAACAAAA‑CGGCGGGGCCCCTGCACAAACGGG‑CACGGCCAGCCATCACCGCAACGCCGGGAGGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGcc                                                                                                             <  1:615788/218‑1 (MQ=255)
                                                                                                                                  aTG‑GAAAACAGGCTCCCGTTAACACCCCGAAAAACAAA‑CTGCCGGTCCCCTGCACCAACTGT‑CACGGCCAGCCATCCCCGCAACCCCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAg                                                                                                           <  2:7057/238‑1 (MQ=255)
                                                                                                                                           cttcttttctttctaaccccAAAAAACAAA‑CGGCCGGGCACCTGCCCCAACTGT‑CCGGGCCGCCCACCCCCGAAACCCCCGGAAGGGGTAAAAGATGGA‑GTGCGTTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTc                                                                                                  <  1:592132/222‑1 (MQ=255)
                                                                                                                                                 tCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGGGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTCCTGGCCGCCCGATGTCCCCGTAACCAAAGAGGCGGCCGCCACATCCCCTTCCCTGc                                                                                             >  1:639817/1‑238 (MQ=255)
                                                                                                                                                 tCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTAGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCCGCTGCCATTCCCTGCa                                                                                            >  2:597878/1‑239 (MQ=255)
                                                                                                                                                   gtttATCACCCCGAAAAACAAA‑CGGCTGGTCGCCTGCCACAACTTA‑CCGGGCCGGCCACCCCCCGAACACCGCGAAGGGGTAAAAGATGTG‑ATGCTCTTTACCGGGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATc                                                                                          <  1:560783/237‑1 (MQ=255)
                                                                                                                                                   cttatccacccccAATAATAAC‑CTGCCGGCCCCCTGCAACACATTC‑CACGGCCAGCCAACCCCACAACCCCGAGAAGGGGTATAAGATGTG‑ATGCTATTGAACGAGCCGATTTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTACCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATc                                                                                          <  1:218627/230‑1 (MQ=255)
                                                                                                                                                     tttccAACCCGAATACCACA‑CTGCCGGTCCCCCGCCCCAATGGC‑CACGGCCAGCCAGCACCGCAACCCCGCGAGGGGGTGAAA‑AAGTGAATGCTCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGGCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCca                                                                                        <  1:378476/235‑1 (MQ=255)
                                                                                                                                                     ttATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATcc                                                                                         >  2:476948/1‑238 (MQ=255)
                                                                                                                                                     ttACCACCCCGAA‑AACAAACCCGCCGGGCCCCCGCCCCAAGTGC‑CACGCCCCCCAACCCCCCCAACCCCGGGAGGGGGGAAAAGATGGG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCca                                                                                        <  1:476948/239‑1 (MQ=255)
                                                                                                                                                          aaCCCGAATAACAAC‑CGGCCGGGCCCCTGCACCAACTGG‑CACGGCCAGCCATCACCGGAACACCGGGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCa                                                                                   <  2:827766/239‑1 (MQ=255)
                                                                                                                                                            cccGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAg                                                                                 >  2:808764/1‑239 (MQ=255)
                                                                                                                                                               gAATCACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAAGG‑TGGAGAGCAGCACAGCGTCTGTATGTCCTGTCATCTGCCGGGACAGATGCAAAAAACGTTCTGGCGCCACGATGCGCCCGTCACCAACGTGGGGTGCGCCAGCTGCCCTTCCCTGCATCCAAAACAAGATa                                                                              >  1:732464/1‑239 (MQ=255)
                                                                                                                                                                aaTAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAACGTTCTGGCCGCACGATGTCCCCGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCCCAGCAAGaaaa                                                                             >  1:195140/1‑236 (MQ=255)
                                                                                                                                                                     accaagcgCCGGCCACCTGCCCCAA‑TGTCCAAGCCCAGCCATCACCGCAACACCGCGAAGGGGTAAAAGATGTG‑ATGCGTTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTTTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATg                                                                         <  2:25599/232‑1 (MQ=255)
                                                                                                                                                                               ggTCCCCTGCCCCAATTGT‑CCCGGCCGTCCATCCCCGCAACCCCGCGAGGGGGGAAAAGATGGG‑ATGCGCTTTACCGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTaa                                                               <  1:203756/239‑1 (MQ=255)
                                                                                                                                                                                 tCACCGGCCCCAACGGC‑CCCGGCCGGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGGG‑AGGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAg                                                              <  2:316816/238‑1 (MQ=255)
                                                                                                                                                                                        cACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGCCGTTAAGGGACAAAg                                                      >  2:359858/1‑239 (MQ=255)
                                                                                                                                                                                          ccAACTGT‑CCCGGCCAGCCATCACCGCAACAGCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGa                                                    <  2:567552/239‑1 (MQ=255)
                                                                                                                                                                                          acAACTGA‑CACGGCCAGCCATCCCCGCAACCCGGGAAAGGGGTGAAGGATGTG‑TTGCGCTTTAACGAACCGATGTACAAGGT‑TGGAGAGCATAACTGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGTCCGCACGATGTCCACGTAACCAAAGTGGCGTGCACCAGCTGGCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGa                                                    <  2:392466/238‑1 (MQ=255)
                                                                                                                                                                                           cAAATGT‑CCCGTCCAGCCCTCTCCGCACCACAGCTAATGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATTTACAAGGT‑TGGAGAGCAGAACAGCCTCTGTATGGCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGGCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGAc                                                   <  2:517610/239‑1 (MQ=255)
                                                                                                                                                                                             aCTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGCAGTTAAGTGACAAAGGCCgg                                                 >  2:559944/1‑239 (MQ=255)
                                                                                                                                                                                             aCTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACgg                                                 >  1:67749/1‑239 (MQ=255)
                                                                                                                                                                                               tGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGCGAAAGATGTG‑CTGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACAATGTCCACGTAACCAAAACGGCGTGCCCCAGCTGCCCTTCCCTGCATCCACCGCAAGATACCTTGCCGACGTTAAGTGACAAAGGCCGGAt                                               >  1:57690/1‑239 (MQ=255)
                                                                                                                                                                                                           cccaTCACCGCAACACGGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCg                                    <  2:281840/238‑1 (MQ=255)
                                                                                                                                                                                                            ccaTCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAATCGTTCTGTCCGCACGATGTCCACGTAACCCAAGTGGCCTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACAATGCAAACGTTAAGTGACAAAGTACGACTTCAGATTTTCg                                    >  2:344771/1‑238 (MQ=255)
                                                                                                                                                                                                               cccccGCAACGCCGCAGGGGGGTAAAAGTTTTG‑AAGCGTTTTAACGGGCCGATTTACAAGGT‑TGGAGTGCAGAACAGCGTGTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGa                                <  1:407975/236‑1 (MQ=255)
                                                                                                                                                                                                                caccgcgacaccgcgaAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGAt                               <  2:583182/239‑1 (MQ=255)
                                                                                                                                                                                                                  ccGGAACACCGGGAAGGGGTAAAAGATGTG‑ATGCCTTTAAACGGGCCGATTTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTg                             <  2:57690/239‑1 (MQ=255)
                                                                                                                                                                                                                  ccGCAACCCCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTg                             <  2:180296/239‑1 (MQ=255)
                                                                                                                                                                                                                      aaCACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTCCATCCCCCGCAAGATACGATTCAGACGTTAAATGACAAAGGACGGATTAAGATTTGCGTGGAATGCcaa                         >  1:769858/1‑238 (MQ=255)
                                                                                                                                                                                                                          ccGGGAAGGGGTAAAGGGTTGG‑ATGCGTTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCg                     <  2:205413/239‑1 (MQ=255)
                                                                                                                                                                                                                           cgcgAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAGGGACGGATTAAGATTTGCGTCGATTGCCCCAGCGa                    >  2:584211/1‑239 (MQ=255)
                                                                                                                                                                                                                             cgAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCGAACGTTAAGTGACAAAGGCCGGATTAAAATTTGCGTCGATTGCCAAAACGaca                  >  1:81967/1‑237 (MQ=255)
                                                                                                                                                                                                                             cgAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACg                                                                   <  2:676836/190‑1 (MQ=255)
                                                                                                                                                                                                                             cgAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGAGTTGGGTCAATTGCCACACCGATc                  >  2:115255/1‑239 (MQ=255)
                                                                                                                                                                                                                                     tGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCAc           <  2:573138/238‑1 (MQ=255)
                                                                                                                                                                                                                                       aaaGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGTCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGATGCCATTCCCTGCATCCCCAGCAAGAAACGATGCAAACGTTAAGGGCCAAAGGCCGGATTAAGGTTTGGGCCGATTGCCACAACGacaaggcaaccaa        >  1:828344/1‑227 (MQ=255)
                                                                                                                                                                                                                                            tgtg‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGa   >  2:136517/1‑239 (MQ=255)
                                                                                                                                                                                                                                             gtg‑ATGGGGTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGGCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCg    <  2:195140/237‑1 (MQ=255)
                                                                                                                                                                                                                                             gtg‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAACAGCGTTCTGGCGGCACGATGTCCACGTAACCAAAGTGGCTTGCGCCAGCTCCCATTCCCTGCATCAACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGAATTAAGATTTGCGTCGAATGCCACAGCTATCCACAACCCAATCCGaa  >  2:127455/1‑239 (MQ=255)
                                                                                                                                                                                                                                             gtg‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACACAGGCCGGATTAAGATTTGCGTCGTTTGCAACCGGCATCAACGCACAAACCCCaa  >  1:69624/1‑239 (MQ=255)
                                                                                                                                                                                                                                              tg‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGa   >  2:590791/1‑237 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               
TTGTGGAGCCTGAACGGAATTACCGCTACCCCTGCGGCGCAGGCATCTGATGATCGCTACGAAGTTACCCAGCAGCGTAACCCGGATGCCGCCTGTCTGGACTGTCATAAACCAGATACCGAAGGTATGCATG‑GAAAACATGCTTCCGTTATCAACCCGAATAACAAA‑CTGCCGGTCACCTGCACCAACTGT‑CACGGCCAGCCATCACCGCAACACCGCGAAGGGGTGAAAGATGTG‑ATGCGCTTTAACGAGCCGATGTACAAGGT‑TGGAGAGCAGAACAGCGTCTGTATGTCCTGTCATCTGCCGGAACAGTTGCAAAAAGCGTTCTGGCCGCACGATGTCCACGTAACCAAAGTGGCGTGCGCCAGCTGCCATTCCCTGCATCCACAGCAAGATACGATGCAGACGTTAAGTGACAAAGGACGGATTAAGATTTGCGTCGATTGCCACAGCGATCAGCGCACCAATCCGAA  >  NC_000913/4289295‑4289767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑