Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,316,228 A→C 22.0% V331G (GTG→GGG)  phnM ← ribophosphonate triphosphate hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,316,2280AC22.0% 125.0 / 2.3 59V331G (GTG→GGG) phnMribophosphonate triphosphate hydrolase
Reads supporting (aligned to +/- strand):  ref base A (35/9);  new base C (4/11);  total (39/20)
Fisher's exact test for biased strand distribution p-value = 3.77e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCC  >  NC_000913/4315994‑4316449
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             
cTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAACATGATTGTCCTTGCGATGCGCCACCCCCAGGTCGGAGCGGTTGCCCTTGCCAATAACCCCGCGATCCTGGCGGGTAAGCGCCTGGGCTGGGTTTTTACTCCCCa                                                                                                                                                                                                                            >  1:181059/1‑238 (MQ=255)
 tGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCCCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTCGTCCCCa                                                                                                                                                                                                                            >  2:585538/1‑237 (MQ=255)
     tttGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGTGTTTTAATCACCAGCTTc                                                                                                                                                                                                                       >  1:528902/1‑238 (MQ=255)
                            ccccggcgcttttttttCCCGGGTCCGGGCGCCCCCATTACCAAAATCAGTTTTCCCAACCACAGAACACCCGTTTCCCCGTACGCCAGACGGGGTCGATATGAAGATGGTTTGCCTTGCGGTGCGCCAGCCCCAGGTCGGCGCGTTTCCCCTCGCCAATCCCCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCgg                                                                                                                                                                                               <  1:341182/225‑1 (MQ=255)
                                  gggCTTTCTTTCCCGGCCCCGGCCGCCCCCATTACCCAAACCAGTTTTCCCATCAACAGAACCCCCTTTTCCCCTGACGCCAGACGGGGTCGATAGGAATATGATTGCCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCCCCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGct                                                                                                                                                                                          <  1:81942/237‑1 (MQ=255)
                                                  aGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCCCCTGCGCTGGATTTTTAGTCACCAGCTTCCCCGCCTGCGGCAGCGTAAAGCGGGTGGTCTGGTCACCGGCGCcgg                                                                                                                                                                         >  1:846816/1‑238 (MQ=255)
                                                    ccGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGCAAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGa                                                                                                                                                                       >  1:15089/1‑239 (MQ=255)
                                                      ggACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGaaa                                                                                                                                                                     >  2:543682/1‑239 (MQ=255)
                                                             cccTTTACCCAAATCATTTTTCCCTGCATCAAAACCCCCTTTTACCCGGACCCCAGACGGTGTGGATATAAATATGTTTGCCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCa                                                                                                                                                              <  1:427662/239‑1 (MQ=255)
                                                                          tccttttcCCCTTCACAAGACCACCTTTTTCCCCGGCCCCGAGACGGGGGCGATAGGAATTGAATTTCCCTTCCAGGGCCCCACCGCCGGGGCGGCGCTTTGGCCCTCGACCATCACCCCGGGATCCTGGAGATTAGGCGCTTGCGTGGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTgg                                                                                                                                                 <  1:645756/235‑1 (MQ=255)
                                                                                 tCCCCTCATCAAAACCCCTTTTTCCCCTCGCGCCCGCCGTGGTCGTTATGAATTTGTTTGCCCTTGCGATGCGCCAGCACCAGGCCGGCGCGTTTGCCCTCGCCAACCACCCCGGGACCCTGGAGATTAAGCCCCTGCGGTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGgta                                                                                                                                          <  1:369246/239‑1 (MQ=255)
                                                                                        aaccgAACCCCCTTTTACCCGGGCGCCAGCCGTGGTCGATATGAATATGATTGACCTTGCGAGGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCg                                                                                                                                   <  2:152511/235‑1 (MQ=255)
                                                                                            aaccgcctttttGCCCTGACGCCGGGCGTTGTCGATGTTAATATGTTGTGCCTTGCAACGCGCTAGCACCAGGTCGTGTCGTTTGACCTCGCCAATCACCCCGCGTTCCTGGAGATGAGACGCCTGCTTTGGATTTTAAGGCACAAGCTTCAGCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAag                                                                                                                               <  1:501786/231‑1 (MQ=255)
                                                                                             aaCACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGCAATGCCGCCTCGAGCAGGCTGGCGGGGGTGTAGTCGGaaca                                                                                                                              >  2:254829/1‑237 (MQ=255)
                                                                                               cccccTTTACCCCGGACGCCGGGCTGTGTCTATTTGAATATGTTTGCCCTTGCGATGCCCAAGCCCCAGGTCGGCGCGTTTGCCCTCGCCAATCCCCCCGCGGTCCTGGAGATGAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAagaga                                                                                                                            <  1:105725/237‑1 (MQ=255)
                                                                                               cacCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGCGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCCCCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAACTGCCGCATCCAGAGGGGGGGCggggggggaagcggaggagg                                                                                                                            >  1:784710/1‑223 (MQ=255)
                                                                                                  ccTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGGTCATCTGGTCAACGGCCACGCGAAATGCCGCATCGCACCGGCTGGCGGGGTCGATGTCggcacgaagga                                                                                                                         >  1:50458/1‑230 (MQ=255)
                                                                                                  ccTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGTCGACGCGAAATGCCCCATCGAGCAGGCTGGCGGGgtcttatacggagaacaggg                                                                                                                         >  2:501786/1‑221 (MQ=255)
                                                                                                  ccTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGAGCCGAAATGCCGCCCCGAGAAGGCTGGCGGGGGAGTAGGCGGGCGAGAGGa                                                                                                                         >  1:250370/1‑239 (MQ=255)
                                                                                                  ccTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTGGTAGGCGGGAGAGAGGa                                                                                                                         >  2:804206/1‑239 (MQ=255)
                                                                                                    ttttACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGTGCATCGGCGCCCCGAAATGCCCCATCGAGCAGGCTGGCGGGGGGGTAGGCGGaaaaaggggaa                                                                                                                       >  1:647367/1‑230 (MQ=255)
                                                                                                       tACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGACCAGGCTGGCGGGGGAGAAGGCGGAAGAGAGGAAATcc                                                                                                                    >  1:91492/1‑239 (MQ=255)
                                                                                                       tACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAACCGGTTGCTCTGGTCATCGGCGACGCGCAATGCCGCATCGAGCAGGCTGGCGGGGTAGAAGTCGGAagcgggggaatcc                                                                                                                    >  2:291422/1‑228 (MQ=255)
                                                                                                            tGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCCCATCGAGCAGGCTGGCGGGGTAGGAGTCGGAAGGCGGGGTATCcaacc                                                                                                                >  2:837892/1‑235 (MQ=255)
                                                                                                             gACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGCCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGGGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGCAGGCGGAAAAGAGGATATCcag                                                                                                                  >  2:444661/1‑235 (MQ=255)
                                                                                                                   aGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCCTCGGCGCCCCGAAATGCCGCCTCCAGCAGGCTGGCGGGGTAGTAGTCGGAAAAGAGGAACCCCAAcaagccaagc                                                                                                        >  1:754952/1‑239 (MQ=255)
                                                                                                                          gTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTTCGccc                                                                                                 >  2:81813/1‑238 (MQ=255)
                                                                                                                                     tatgaTTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACCCGAAATGCCGCATCGGGCAGGCTGGCGGGGGGGTAGCTGGAAAGGAGGATATCCAGAAGGCCAAACCGCGcccagcaaccggc                                                                                       >  1:387173/1‑227 (MQ=255)
                                                                                                                                         aTTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGCAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGgccgcc                                                                                   >  2:192342/1‑238 (MQ=255)
                                                                                                                                         aTTGCCCTTGCGAGGCCCCAGCGCCAGTGCGGCGCGTTTGCCCCCCCCAATCCCCCCGCGATCCGGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCa                                                                                  <  2:181059/239‑1 (MQ=255)
                                                                                                                                             tCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGtt                                                                              >  2:472372/1‑239 (MQ=255)
                                                                                                                                             tCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGGTCACTGGCCGCCACGtt                                                                              >  2:168910/1‑239 (MQ=255)
                                                                                                                                             tCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGGGGGAAGCGGGAAAGGGGAAATCCCACAGGCCAAGCGGGGCCCGTTCACCGGCCCCCACggt                                                                              >  1:718514/1‑237 (MQ=255)
                                                                                                                                                cTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCCCGTTg                                                                             >  2:579710/1‑238 (MQ=255)
                                                                                                                                                         cgcCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGCAGTAGTCGGAAGAGAGGAAATCCAGCAAGc                                                                                                             >  2:67213/1‑197 (MQ=255)
                                                                                                                                                              gccccgggTGGGCGCGTTTGCCCTCGCCAATCACCCCGGGATCCTGAAGATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTCCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAgccg                                                              <  2:442734/233‑1 (MQ=255)
                                                                                                                                                              gCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGCAATGCCGCATCGAGCAGGCTGGCGGGGGAGTAGTCGGAGGGGAGGATATCCAGGAGGCCAAGGTGCGCCAGTTCACTTGCCGCCCCGTTGCCGGAGTGCGAggc                                                               >  2:445427/1‑236 (MQ=255)
                                                                                                                                                                cccAGGGCGGCCGGTTTGCCCTCGCCAATCCCCCCGCGATCGTGGAGATTAAGTCCCTGCGCTGGATTTTTAGCCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAgccgcc                                                            <  2:91492/238‑1 (MQ=255)
                                                                                                                                                                    ggTCGGCGCGTTTGCCCCCCCCAAACCCCCCGGGATCCTGGAAATTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCgcgc                                                        <  2:828766/239‑1 (MQ=255)
                                                                                                                                                                      tCGGCGCTTTTGCCCTCGCCAATCACCCGGCGATCCTGGAATTTAAGCGCCTGCGCTGGATTTTTAGTCCCCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGca                                                       <  2:528902/238‑1 (MQ=255)
                                                                                                                                                                          cgcgTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGGAGGAGTCGGAAGGAAGGGAATCCAGCAGGGCCACCCGGGCCCGTTCCCGGGCCCCCCCGTTTCCggggggggggccccccgccccaaaa                                                  >  1:774915/1‑216 (MQ=255)
                                                                                                                                                                          cgcgTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGCCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAAAGGCAATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGGATGCGAGCCGCCGCGCCCaaaa                                                  >  2:196743/1‑237 (MQ=255)
                                                                                                                                                                                tGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTTGCCGCCCCGTTGCCGGAGGGCGAGCCGCCGCGCCCAATATTCgc                                             >  2:579917/1‑237 (MQ=255)
                                                                                                                                                                                   ccctcccAACCCCCCCGCGATCCTGGGACTTAAGCGCCTGGGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGgcgcg                                         <  2:754952/234‑1 (MQ=255)
                                                                                                                                                                                      cgcaaactcccccGCGATCCTGGAAATTAAGCGCCTGCCCTGGATTTTGTGGCCCCAGGTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGccc                                      <  2:566014/230‑1 (MQ=255)
                                                                                                                                                                                           atccccgctcgATCCTGGGGTATAGGCGCTGGCGTCTGGTTTTTGGGCACCGGCTACACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCAGCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAg                                 <  2:264411/230‑1 (MQ=255)
                                                                                                                                                                                            cccccccGGGATCCGGGAGTTTAGGGGCCGGCGCGGGATTTTTAGTCCCCAGCTGCCCCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGc                                <  2:647367/236‑1 (MQ=255)
                                                                                                                                                                                             ccccccggagcccgggAAATTAAGCGCCTGGCCTGGATTTTTAGGCCCCAGCTGCGCCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCa                               <  2:15089/225‑1 (MQ=255)
                                                                                                                                                                                                 cggctaTCCGGGAGATTAAGCGCCTGCGTTGGATTTTTAGTCCACGTGTGCCCCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGtt                           <  2:618634/234‑1 (MQ=255)
                                                                                                                                                                                                          tGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGCTATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTcctgccca                   >  2:843326/1‑236 (MQ=255)
                                                                                                                                                                                                                 tAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCCGGCACAATATTCGGCGCGCCCATCCGCACGTTCATGCCATGCTTccgc           >  2:851526/1‑239 (MQ=255)
                                                                                                                                                                                                                 tAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGGGCGAGCCGCCGCGCCCAATATTCGGCGCGCCCATCAGCCCGTTCATGCCCTGCTTgcgc           >  2:468023/1‑239 (MQ=255)
                                                                                                                                                                                                                       cttGCTCTGGATTTTTGGCTCCCAGTTTGACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGc      <  2:250370/236‑1 (MQ=255)
                                                                                                                                                                                                                         tGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCCTCAGCACGTTCATGCCATGCTTCGGCGAGGcgt    >  2:450187/1‑236 (MQ=255)
                                                                                                                                                                                                                         tGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCCCGTTcatgccatgc                 >  2:167873/1‑225 (MQ=255)
                                                                                                                                                                                                                         tGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCCCGTTCATGCCATGCTTGCGCGAGGCtt    >  2:112361/1‑238 (MQ=255)
                                                                                                                                                                                                                         tGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGGAGTAGTCGGAAGAGAGGATGTCCAGCAGGCCAAGCTGCGCCAGTTCACTCGCCGCCCCGTTGCCGGCGGCGGGGCCCCCGCGCCCCAAATTCGGCGCGCCCATCAAGACGGTACTGCCAAGCTTGGGCGAggggtc   >  1:295907/1‑235 (MQ=255)
                                                                                                                                                                                                                          gcgcTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACACTATTCGGCGCGCCCATCAGGACGTTCAGGCCATGCTTGCGCGGGGCTTc   >  2:296868/1‑238 (MQ=255)
                                                                                                                                                                                                                           cgcTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGGTTcc  >  2:760849/1‑238 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                             
CTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCC  >  NC_000913/4315994‑4316449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: