| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,579,657 | A→C | 23.7% | V62G (GTG→GGG) | symE ← | toxic peptide regulated by antisense sRNA symR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,579,657 | 0 | A | C | 23.7% | 90.5 / 3.7 | 54 | V62G (GTG→GGG) | symE | toxic peptide regulated by antisense sRNA symR |
| Reads supporting (aligned to +/- strand): ref base A (36/3); new base C (5/9); total (41/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG > NC_000913/4579424‑4579889 | gtaattttttgaggggggggAAATTTTTTTGTGAGTCGTGGGAAGTCCCCCGGGGGTTAACCGGGAGTAACCCGTTTTACGCTACTTTCTGTTTACCGGCAATCCCCCCAAA‑AACCGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACCCCGGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTg < 1:358765/224‑1 (MQ=255)gTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGCC‑AATACAGCCTTCCATCACTTTg > 2:610974/1‑239 (MQ=255)gTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTAAGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGGAGGGAC‑AAAAACGCCTTCCACAACtttt > 1:730179/1‑238 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCCGTTCGCTCTCCTCGGCGGAGGGTGGGTGGGCGGGGGGGCC‑AATACAGCCCTCCATCACTTTG‑AAATCTAccc > 1:618761/1‑238 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCCATTTG‑ACAttaacc > 2:180033/1‑233 (MQ=255) aCTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGGTGGGCGGGTAGGAC‑AATAAAGCCTTCCATCACCTTG‑GCATCTAcc > 1:69914/1‑238 (MQ=255) gAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCCCTTTG‑ACATCTCccgccg > 2:374625/1‑239 (MQ=255) gggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACAACAACCGCCGt > 2:874211/1‑238 (MQ=255) gggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACACTTTGCACACCTGGCGTAGCGACTGCCTCAGTTCGCTCCCCTCGGGGGCGGGGGGTTGGGCGGTAAGGCC‑AACACAGCCTTCCACCCACTTG‑AAATCTAcccccgg > 1:526232/1‑237 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGGGGGGGGGTGGGGGGGAGGGCC‑AATAAAGCCTTCCCCCCCTTTG‑ACATCACCCGCCGTg > 1:467750/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGGC‑AATACAGCCTTCCATCACTTTG‑CCATCTACCGCCGTg > 2:212738/1‑238 (MQ=255) ggggAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTg > 2:710451/1‑238 (MQ=255) ggggAAAGTTTCTACTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGGGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTg > 2:750574/1‑238 (MQ=255) tCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGCC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCaaa > 2:570379/1‑239 (MQ=255) gTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGGGGCAAAACcggcg > 2:279026/1‑238 (MQ=255) gTCAGTGGAAGGCTACCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCATT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGCCAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGCGGTTGGGCGGCGCGTCC‑AATACAGCCTTCCTTCACTTTG‑ACATCTACCGCCGTGCCAGTAGCAAAACcggcg > 2:125741/1‑238 (MQ=255) cccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCCCCGTGCCAGTGGCAAAACCGGCGGCTTCCAACCCCTg > 2:460628/1‑238 (MQ=255) ccGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCCTCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCATCCCCTGa > 2:731156/1‑238 (MQ=255) ccGGGAGTAACTCCGTTTACGCGTCTTTTGTTTTACCGCAAATCACCCAAAA‑AAACCCCTGCCCCTGTTTTTGTTTCCGCCCCGGCAGTTGGCACACTGGGGGGAGCGACTGAACCAGTCCGCTCTCCCCGGCGGCGGGTGGTGGGGCGGTGAGGAA‑AATCCAGCCTCCCATCACTTTG‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTg < 1:523816/239‑1 (MQ=255) aGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCAAGCCACTGACCTTTCAGGGTGATGGc > 2:233168/1‑239 (MQ=255) aGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGCGTGGGCGGGACGGAC‑AATCCAGCCTTCCATCACTTTG‑TCATCTACCGCCGTGCCCGGGGCAAAACCGACGGCTTCCACCCAATGACCCTTCAGGGTAATgac > 1:176042/1‑237 (MQ=255) cctttttccctttttttttttctccGTCAATC‑CTCCAAAAAAACGCCCGCCCCTTTTTTTGGTTCCGCGCCGACTGTTGGCCCCCCGGGCGTAGCGTCAGAATCAGTTCGCTCCCCTCGGCGGCGGGGGGTGGGGGGGGGAGGAA‑AATACAGCCTTCCATCCCTTTG‑CCATCTCCCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATa < 1:460628/216‑1 (MQ=255) tGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑CCATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCACCCACTTACCTTTCCGGGGGATgcgggggaaaag > 1:802403/1‑228 (MQ=255) ttCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCCCTGACCTTTCAGGGGGATGGCGGGAATACG‑GCTGTAATCCgg > 2:445083/1‑239 (MQ=255) aCCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGGATACG‑GCTGGAATCCGGGTGGCgg > 2:452424/1‑237 (MQ=255) aCCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGAGGGCGGGAAAACGCGCTGAAAACCGGGGAgga > 1:422850/1‑236 (MQ=255) aCCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCACCCACTGCCCTTTCCGGGGGCGGGCGGGGAAACG‑GCCGTAATCCGGGGGGCGa > 1:880184/1‑238 (MQ=255) aCCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGATTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACAGCATCAGTTCGCCCTCCTCGGCGTCGGGGGGTTGGGCGGTGAGGGC‑AATGCAGCATTCCATCCCTTTG‑ACATCTACCGCCGTGCCAGTGGCGAAACAGGAGGCTTCCAGCGCATGACCTTTCCGGGGGAGGGCGGGATAACG‑GCTGGAATCCgggggggca > 1:662247/1‑232 (MQ=255) cAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGCGGGGAATACG‑GCTGTAAACCCGGGAGCGAccccca > 1:332963/1‑234 (MQ=255) gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGCCCTTTCAGGGTGATGGGGGGAATACG‑GCCGTAATCCGGGTAGCGACTCGCaaaaaccgggggggcaag > 1:747405/1‑225 (MQ=255) gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGGTGGCGGGGATAAG‑GCTGTAAACCGGGCAGCCACCCGCCAAAACG‑ACGGGGaaaaa > 1:102816/1‑235 (MQ=255) gCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGGGaaccccaaaaccaccgggggcctt > 2:715111/1‑216 (MQ=255) gCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGGAATCCGGGTAGCGACTCGCATAACCA‑ACGGTGACATGACgg > 2:186802/1‑239 (MQ=255) gCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGAGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGGATACG‑GCTGGAATCCCGGGAGGGACCCGCCTaaaccgcgggggaatcaggg > 1:602589/1‑221 (MQ=255) cACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCAAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGAACCCCTTAACCG‑ACGGTGACATGACGGt > 2:125141/1‑239 (MQ=255) cccggCTTTTGTTTACGGCCCGAAAGTTTGCCACCCGGGCGAGGGCAAGGCATCAGTTCGCTCTCCGCGGGGGGGGGTGGTTGGGGGGTGAGGAA‑AATACAGCTTTCCATCACTTTG‑ACATTTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGtt < 2:571765/235‑1 (MQ=255) tGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGCCGGTTAtt > 2:416819/1‑239 (MQ=255) tGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCgg > 2:130179/1‑239 (MQ=255) tGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATGCAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGGGATGGCGGGACTACG‑GCTGTAATCCGGGTAACGACTCGCATAAACGAAGGGGGACGAGA‑GGTTTTTTGCCGGGGGAACTTCCGGTTCGAAc > 1:336741/1‑239 (MQ=255) tGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACTTCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGGGACATGACGGTTATTTGCCGGGGGGACTTCTGCTTCGAACGGtttggc > 2:509635/1‑235 (MQ=255) gagcggCATCAGTTCCGTCCCCCGGGCGGGGGGTGGTTGGGCGGTGAGGAA‑AATCCAGCCTCCCATCCCTTGG‑ACATTTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAAt < 2:102816/234‑1 (MQ=255) gcagtaGTTCGCTCGCGTCGGCGGGGGGTGGTGGGGCGGTGATGAC‑AATCCAGCCTTCCATCCTGTTG‑ACATCTACCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCgtg < 2:440662/234‑1 (MQ=255) gTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCACGTGGCAAAACCGGCGGCTTCCAGCCCCTGCCCTTTCAGGGTGATGGCGGGAATACG‑GCCGTAATCCGGGTCGCGACTCCCCTAACCC‑CCCGTGCCATACCAGTTATTTTCCGGGGGGACGTCTGCGTCGCACGGGTGTGCAACAGAAAGCgtgtgcggg > 1:430592/1‑234 (MQ=255) ctggggggggggTTGTTGGGGGGTAGGGCA‑AATCCACCCTTCCACCCCTTGG‑ACTGCTCCCGCCGGGGCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTa < 2:430592/230‑1 (MQ=255) gcgggggTTGTGGGGGGGTG‑GGACAAATCCCGCCTTCCA‑CCCTTTGCACTTCACCCGCCGGGCAAGGGGCAAAACCGGCGGCTGCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATtctc < 1:710451/235‑1 (MQ=255) ggcggGTGGTGGGGGGGTGAGGAC‑ATTACAGCCTTCCACCCTTTGG‑ACATCTCCCGCCGTGCCAGTGGCAAAACCGGCGGCTGCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATtctc < 2:764659/238‑1 (MQ=255) ggtgttTGGGCGGTGAGGAC‑AATCAACCCTCCCATCCCTTTG‑CCATCTACCGCCGGGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAgg < 2:400553/238‑1 (MQ=255) ggtggtTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTTTGCAATAGCATGCGTGTCAGTCATAACTGCTATTTTCCAGGa > 2:464561/1‑239 (MQ=255) ggggaaGGAA‑AATCCAGCCTCCCATCCTTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAgtgatt < 2:513305/233‑1 (MQ=255) ggggtaggaaaatcacgCCTTCCATCTCTTTG‑CCATGTGCCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAgtgatt < 2:618761/222‑1 (MQ=255) aGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCCTAACCG‑ACGGGGACATGCCGGTTATTTGCCGGGGGGACTTCTGCTTTGAAAGGTTGGGCAATAGAATGCGTGTCAGTCATAACAGCCATTCCCCAGGAAAAgggattgggatt > 1:695099/1‑239 (MQ=255) aaaaatcAGCCTTCCACCCATTGG‑AACTCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAg < 2:832193/232‑1 (MQ=255) ccTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGgt > 2:487995/1‑239 (MQ=255) tCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGGGACATGACGGTTTTTTGCCGGGGAGACTTTTGCTTCGAACGGTTGTGCAAAAGAATACGGGTCCGGCATAAATTCCATTATCCAGGAAAAGTGATTGAGAATAACGAAGCgggggga > 1:294727/1‑235 (MQ=255) | GTAAATATTTACTGAGGGGGAAAGTTTCTATTGAGTCAGTGGAAGGCTCCCGGTGGTTAACCGGGAGTAAACGCTGTTACGCGACTTTCTGTTTACCGGCAATCACTCCAAT‑AAACGCCTGCACCTGCTTTTGTTTACGCGCCGACAGTTTGCACACCTGGCGTAGCGACTGCATCAGTTCGCTCTCCTCGGCGGCGGGTGGTTGGGCGGTGAGGAC‑AATACAGCCTTCCATCACTTTG‑ACATCTACCGCCGTGCCAGTGGCAAAACCGGCGGCTTCCAGCCACTGACCTTTCAGGGTGATGGCGGGAATACG‑GCTGTAATCCGGGTAGCGACTCGCATAACCG‑ACGGTGACATGACGGTTATTTGCCGGGGAGACTTCTGCTTCGAACGGTTGTGCAATAGAATGCGTGTCAGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTG > NC_000913/4579424‑4579889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |