Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,619,955	T→C	15.6%	S118P (TCC→CCC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,955	0	T	C	15.6%	201.0 / 5.0	94	S118P (TCC→CCC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base C (3/14);  total (41/53)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAAT  >  NC_000913/4619720‑4620190                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             cAAAGGGGAAGCTGATACCGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGCCCCGTCTGGGGCTGGCGAAAGCAAACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAAGTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCg                                                                                                                                                                                                                                           <  2:421962/239‑1 (MQ=255)   aaGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGAt                                                                                                                                                                                                                                         <  2:490661/239‑1 (MQ=255)       cGAAGCTGATAACTGTAGTAAAGGCCCGCGCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCCCACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AATGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCAc                                                                                                                                                                                                                                      <  2:121127/238‑1 (MQ=255)          aGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTTTCCGATCACGaaa                                                                                                                                                                                                                                  >  2:102319/1‑239 (MQ=255)                tAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCt                                                                                                                                                                                                                            <  1:72036/239‑1 (MQ=255)                    ggTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCGGTTTGAGGGGGG‑GTATTTTTCCGATCACGAAAAACGCTTcc                                                                                                                                                                                                                         >  2:695072/1‑238 (MQ=255)                      tCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGG‑AAAGATACCCC‑GGCTGGTCACTGGGGAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑AAATTTTCCCGATCCCGACAACAGCTTCCCGc                                                                                                                                                                                                                      >  2:209403/1‑239 (MQ=255)                       cGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCCCGAAAACAGCTTCCCGCa                                                                                                                                                                                                                     >  2:21422/1‑239 (MQ=255)                         tAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGGCGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGG‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGGGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCCGTCCCGAAAAAACACTCCCGCAag                                                                                                                                                                                                                   >  1:452267/1‑239 (MQ=255)                           aaGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCCTGGGCGCACGAAATGTCATCCGGG‑AAAGAAACCCC‑GTCTGGCCACTGGGGAAATGCCGGTGTCCCG‑T‑GTCTGATTGAGTGGGG‑GTATTTCCCCGATCCCGAAAACACCTCCCCCCAagg                                                                                                                                                                                                                  >  1:858465/1‑237 (MQ=255)                               ccctgtCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACAGGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                                                                                                                                                              <  2:857379/233‑1 (MQ=255)                               cccGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                                                                                                                                                              <  1:627777/238‑1 (MQ=255)                               cccGCTCAATCTGCCAAATCTGACCCGTCTGGGGCGGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                                                                                                                                                              <  2:373605/238‑1 (MQ=255)                               cccGCGCAATCTGCCAAATCGGACCCGTCTTGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTTAAGGTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                                                                                                                                                              <  2:530848/238‑1 (MQ=255)                                ccGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGGGGGG‑GTATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgct                                                                                                                                                                                                               >  2:354616/1‑236 (MQ=255)                                   cTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGCAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑CTATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                                                                                                                                                                         >  2:138896/1‑239 (MQ=255)                                        tCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCCCGAAATGTCATCCGGT‑AAAGATACCCC‑GCCTGGGCACTGGGAAATTGCCGGGGTCCCG‑G‑TTCGGTTTTGGGGGGG‑GAATTTTTCCGATAACGAAAACAACTTCCCGCaaaaacgggtgggaaaaa                                                                                                                                                                                                    >  1:420300/1‑223 (MQ=255)                                               aatttgaccctgtttgggCTGGCGAAATCCCACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                                                                                                                                                             <  2:282500/224‑1 (MQ=255)                                               aaTCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                                                                                                                                                             <  2:39366/239‑1 (MQ=255)                                                          tCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGGCGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCAAGGAATGTCATCCGGG‑AAAGATACACC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑GCTAGGTTGAGGGGGG‑GGATTGTTCCGATCCCCAAAAAACCATCCCGCCAGGGCTGTTggcgtcactggcggaacccaggact                                                                                                                                                                                   >  1:763915/1‑215 (MQ=255)                                                          tCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGGGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGGTAAACTGGTCGAACGCGC‑TAAt                                                                                                                                                                                  >  2:642099/1‑238 (MQ=255)                                                          tCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAAAGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGGGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGAAAAACTGGTCGAACGCGC‑Taa                                                                                                                                                                                   >  2:474734/1‑237 (MQ=255)                                                            gggggGTGGCGAACGCACACGAAGGTTCTACCGGTTTCACTCCGGCGGGAATGGACGGAAACGCGGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTg                                                                                                                                                                               <  1:558071/238‑1 (MQ=255)                                                               ggCTGGCGAAAGCACACGAATGTTCTACCGGTTTCCTTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGcc                                                                                                                                                                             <  2:369264/238‑1 (MQ=255)                                                                   ggCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGt                                                                                                                                                                         <  2:206168/238‑1 (MQ=255)                                                                    gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGCAATGTCATCCGGT‑CAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGTTAAACTGGTCGAACGCGC‑TAATCTGCCGGGtt                                                                                                                                                                        >  2:545203/1‑238 (MQ=255)                                                                           caccCGAAGTTTTTCCGGGTTTCCTTCCGGCGGGAATGGACGGCAACGCTGAAGTTTG‑CGGCGCGTACGAATGGGGGTACGAAATGTCATCCGGT‑AAAGATACCCC‑GGCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGt                                                                                                                                                                <  1:144168/239‑1 (MQ=255)                                                                           cacaCGAAGTTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATCCCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGt                                                                                                                                                                <  2:42818/239‑1 (MQ=255)                                                                              acGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGGAATTGCCGGTGTCCCG‑G‑TTCTGGTTGAGGGGGG‑CTATTTCTCCGGTCACGCAAACCGCGTCCCGCCAGAGCTGCCTGGTCAACTGGCCCACCGCGC‑CAATTTGCCCGGTTAcccccggaa                                                                                                                                                              >  1:51746/1‑231 (MQ=255)                                                                                 aaGTTTCTACCTGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACAAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAAc                                                                                                                                                             <  2:124962/236‑1 (MQ=255)                                                                                    ttttttccGTTTTCATTGCGGGGGGAATGGAGGGCAACGCTTGAGTTAG‑CGGGGCGTACGCATGGGCGCACGAAATGTCACCCGGT‑AAAGATACCCC‑GGGTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCCCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCa                                                                                                                                                        <  1:37638/232‑1 (MQ=255)                                                                                    gTTTTAGCGGTTTCATTCCGGCGGGAATGGACGGCAAGCGTGAGGTTGT‑CGGCCGGTAGTCTTGGGCCCACGAAATGTCACCCGGC‑AAAGATACCCC‑GTCTGGTCCCGGGGAAATTGCCGGTGTCCGG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGGGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTAACTCGGTAACTGCCa                                                                                                                                                        <  1:635981/238‑1 (MQ=255)                                                                                     tttttccGGTTTCTTCCCGGGGGGAATGGACGGCAA‑GGTGAAATTTTGCGGCGCGATCGCATGGGCGCACAAAATGCCACCCGGT‑AAAGATCCCCC‑GTCTGGTCCTGGGGAAATGGCGGGTGGCCCG‑G‑TTCTGTTTGAGGGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCa                                                                                                                                                        <  1:817385/232‑1 (MQ=255)                                                                                      tCTACCGGTTTCATTCCGGGGGGAATGGACGGCAACGATGAAGTTAT‑CGGCGCGTACGCATGGGCGAACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTTTTTGAGTGGGG‑ATATTTCTCCTATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTGGACCGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCActc                                                                                                                                                     <  2:763915/239‑1 (MQ=255)                                                                                        tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGGTAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCAccct                                                                                                                                                    >  2:460344/1‑238 (MQ=255)                                                                                        tACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTCCCTCGGTAACTGCCActct                                                                                                                                                    >  2:817385/1‑238 (MQ=255)                                                                                              tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGG‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGCGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGGTAAACTGGTCGAACGCGC‑CAATTTGCCGGGTTAACCCGGGAAAAGCAACCCTTCCGGTa                                                                                                                                             >  1:241925/1‑239 (MQ=255)                                                                                               ttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑TAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑CAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTAc                                                                                                                                            >  2:454464/1‑239 (MQ=255)                                                                                                     cgggggggAATGGGCGGCAACGTTTAGGTTTT‑CGGCGCTTACGAATGGGCGCAAGAATTGTCCGCCGGT‑AAAGATACCCC‑GTCGGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGA‑ATATTCCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCAt                                                                                                                                      <  1:232897/234‑1 (MQ=255)                                                                                                     ccGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCAt                                                                                                                                      <  2:300221/239‑1 (MQ=255)                                                                                                     ccGGCGGGAATGGAAGGAAAGGCGTGAGTTAC‑CGGCGGTGCCCCAGGGGGCCACGAAATGGCATCCGGG‑AAAGATCCCCC‑GTCTGGTCACTGGGAAATTGCCGGGGGCCCG‑G‑TTCTGTTTGAGGGGGG‑ATATTTCTCCGACCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGACCGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCAt                                                                                                                                      <  1:267005/239‑1 (MQ=255)                                                                                                        gcggGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCCCGGAATGTCATCCCGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGGGGCCCG‑G‑GTCTGTTTGGGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGCTAAACCGGTCGAACGCCC‑TAATCTGCCGGGGTACCCCGGTAACTGCCACCCTTCCCGGACCGTCAtccg                                                                                                                                   >  1:498176/1‑236 (MQ=255)                                                                                                        gcggGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCGCTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCAt                                                                                                                                      >  2:105345/1‑236 (MQ=255)                                                                                                           gtaaTGGGCGCCAACGGTGAATTTTG‑CGGGGTGTCGCCATGGGCGCAGAAAATGTC‑CCCGGG‑TAAGATTCCCCGGTCGGGTCCCTGGGAATTGGCCGGTGCCCCG‑G‑TTCTGTTGGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAACCTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTgg                                                                                                                                 <  1:21422/236‑1 (MQ=255)                                                                                                                        aCGCTGAATTTAT‑CGGGGCGTACGCTTGGGCGCCAGAAATGTCACCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATGGCCGGGGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGaa                                                                                                                   <  2:324570/239‑1 (MQ=255)                                                                                                                                gTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGAACGGTACTTCTGGATCAACTGGGGGAAGGGcacag                                                                                                           >  1:606747/1‑238 (MQ=255)                                                                                                                                   aT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGaa                                                                                                        >  2:521697/1‑239 (MQ=255)                                                                                                                                    t‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCCATCTTCCGGTACGGCCATTCTGGATCAACTGGGCGAAAAGCAAATGaaa                                                                                                       >  1:365472/1‑239 (MQ=255)                                                                                                                                         cgcgTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGGCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTTGGCGAAGAGCACATGAAAACCg                                                                                                   >  2:236259/1‑239 (MQ=255)                                                                                                                                           cgTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGTTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACCCTTCCGGAACGGGCATTCTGGTTCAACGGGGGGaaagacaaagcaaaaccgga                                                                                                 >  1:255125/1‑221 (MQ=255)                                                                                                                                           cgTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCCCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGc                                                                                                 >  2:825891/1‑239 (MQ=255)                                                                                                                                             tccgcgggggcccgcaaaaTGTTCTCCGGT‑AAATGTTCCCC‑GGCGGGTCCTCGGAAAATTCCCGGTGTCCCG‑G‑TTTTGTTTGAGTGGGG‑ATATTTTCCCGATCACGAAAACGGCTTCCCGCAAGAGCTGCTGGATAAACTGTTCGACCGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCa                                                                                                <  1:608164/222‑1 (MQ=255)                                                                                                                                                  gtgggcgcagagattttctcccGGT‑AAATTTTCCCC‑GGTGGTTCACGGGGAAATCGCCGG‑GTGCCG‑GTTTCTGTTTGGGTGGGG‑TTTTTTCGCCGTCCAAGAAAACAGCTTCCCGCAAGGGCTGCTGGATAACCTGGTCGAACGCGC‑TAATCTGCGGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGa                                                                                          <  1:354616/219‑1 (MQ=255)                                                                                                                                                  gtGGGCGCACTATATTTACCCCTGT‑AAAGATCCCCC‑GTGCGCCTCCTGGGAAATTGCGGGTGCCGCG‑T‑TTCTGTTTGAGGGGGT‑ATCTCTCCGCGATCCGGAACACAGCTCTCCGAAATGGCTGCGGGATAAACTGGTCAAACGCGC‑TAATCTGCCGGGTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGa                                                                                          <  1:289215/238‑1 (MQ=255)                                                                                                                                                  ctGGGCGCCCGAAATTTCTTCCGTT‑AAAGTTCCCCC‑GTTTGGTCCCTGGGAATTTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACAAAAACCTCTTCCCGAAAGAGCTGCTGGATAACCTGGCCGAACGCGC‑TAATCTGCCGGTTTACCTCGTTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAATCCGGCAAGCCGa                                                                                          <  1:454464/238‑1 (MQ=255)                                                                                                                                                  aggggCGCACGAAATTTCATCCGGT‑AAAGATGCCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCCCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCg                                                                                           <  2:255125/236‑1 (MQ=255)                                                                                                                                                  aTGGGCGCACGAAATGTCATCCGTG‑AAAGATCCCCG‑GTCTGTGCACTGGGAAATGGCCGGTGGCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGa                                                                                          <  2:539740/239‑1 (MQ=255)                                                                                                                                                   tGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGAGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCCTTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCCAt                                                                                         >  1:39432/1‑239 (MQ=255)                                                                                                                                                    gggCGCACGAATTGTAACCGGGT‑AAAGTACCCCC‑GTCTGGTCACTGGGAAATTGCCGGTTTCCCG‑G‑TTCTGTTTGAGGGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGAtt                                                                                        <  2:241925/239‑1 (MQ=255)                                                                                                                                                       cgcACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGGGCACATAAAAAACGGCAAGCCGAttt                                                                                       >  1:756947/1‑237 (MQ=255)                                                                                                                                                            gaaagttCACCCGGT‑AAAGATCCCCG‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGT‑AAATTCCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATAc                                                                                <  2:211464/233‑1 (MQ=255)                                                                                                                                                            gAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAAAGCACAACAAAACCGGCAAGCCCGTTTTCtata                                                                                 >  1:695309/1‑238 (MQ=255)                                                                                                                                                               tttttctCCGTT‑AAATATCCCCT‑GTTTGGTCACTGGGAATTTGGCGGTGGCCGG‑G‑TTTTGTTTGGGTGGGG‑ATATTTCCCGGATCCCGAAAACAGCTTCCCGAAAGGGCTGCTGGATAAACTGGTCGACCGCGC‑TAATCTTCCGGGTTCCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTc                                                                             <  1:209403/233‑1 (MQ=255)                                                                                                                                                                 gTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTcc                                                                            >  2:14768/1‑238 (MQ=255)                                                                                                                                                                      ccGGTAAAAGTTCCCCC‑GTTTGGTCGCTGGGAAATT‑CCGGGCTCCCGTG‑TTCTGTTGGAGTGGGG‑ATATTTCTCCGACCCCGAAACCAGCTCCCCGAAGGAGCGGTTGGATAATCGTGTCGAACGCGC‑TAATCTGCGGGGTTACCTCGGTACCTGCCACTCTGCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGa                                                                       <  1:897920/239‑1 (MQ=255)                                                                                                                                                                          t‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTcc                                                                   <  2:365472/238‑1 (MQ=255)                                                                                                                                                                          t‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGCCGACCGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATATTCAAAACTACCGCGGACCCCg                                                                  >  1:379913/1‑239 (MQ=255)                                                                                                                                                                                    ccc‑GTCGGGTCACGGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTAAGTGGGG‑ATATTTCTCGGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGa                                                         <  2:134088/239‑1 (MQ=255)                                                                                                                                                                                        gTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTTTACCTCCGCTGACTCCGGGTTCCAGAtt                                                       >  2:569629/1‑238 (MQ=255)                                                                                                                                                                                         tCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCCATTTTCTATACCTCCGCCAACTCCGAGTTCCAAATtg                                                      >  1:103157/1‑238 (MQ=255)                                                                                                                                                                                          cTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGTCAAGCCAGTTTTTTAAACCCACGCTGACGCCGTGTTCAACATtgc                                                     >  1:88057/1‑238 (MQ=255)                                                                                                                                                                                          cTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGAATCCGTGTTCCAGATtg                                                      >  2:568903/1‑237 (MQ=255)                                                                                                                                                                                           tGGGCACTGGGAAATTGCCGGTGCCCCG‑G‑TTCTGTTTGAGGGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTTCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcc                                                    <  2:770356/238‑1 (MQ=255)                                                                                                                                                                                             gTCACGGGGAAATTGCCGGTATCGCG‑G‑GTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCACGCCGATTTTCTATACCTCCGCTGACCCCGTGTTCCAGATtgccggc                                                 >  1:233148/1‑239 (MQ=255)                                                                                                                                                                                              tCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCCCATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcctccc                                                >  2:804332/1‑239 (MQ=255)                                                                                                                                                                                               cgccggggtatgtgcgtgtgcccG‑G‑GTTTGTTTGGGGGGGGTATATTTCCCGCACCCCGAAAACAGCTCCCCGCAAGAGCGGCTGGAAAAACGGGTGGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACGGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcctgcc                                                <  1:521697/219‑1 (MQ=255)                                                                                                                                                                                                           gCCGGTGCCCCG‑T‑TTCTGTTTGAGTGGGG‑ATATTGCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACttt                                   <  2:673551/239‑1 (MQ=255)                                                                                                                                                                                                               tctgctcttg‑TTCTTTTTTGGTGGTG‑TTTTTTCTCTAATCACGATCACCGCTCCCCACAAGCGCGTCTGCATAAACTGGTGAACCGCGC‑TATTCTGCCGGGTTAGCTGGGTACCTTCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGGCTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCgg                                <  1:391992/230‑1 (MQ=255)                                                                                                                                                                                                               gttccccG‑T‑TTCTTTTTGTGGGGGG‑ATTTTTTCACAGTAACGAAGACAGTTTCCAAGAAGGGCTTCGGGAAAAACGGGGCGAACGCCT‑TAATCGGCCGTGTTACGTGGTTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGAGTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGt                               <  1:695072/235‑1 (MQ=255)                                                                                                                                                                                                                tgtCCCG‑G‑TTCTGTTTGAGTGGGG‑ATCTTTCACCGATCCCGAAAACGGGTTCCCGCAAGGGCTGCTGGATAAACTGGCCGAACGTGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGt                               <  2:858465/238‑1 (MQ=255)                                                                                                                                                                                                                 tgcccG‑G‑TTCTGTTTGAGGGGGG‑ATATTTCCCCGATCCCGAAAACAGCTTCCCCCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTc                              <  2:723221/236‑1 (MQ=255)                                                                                                                                                                                                                 gtCCGG‑G‑TTCGTTTTGAGGGGGG‑TTATTTCCCCGGTCACGAAAACAGCTTCCCGAAAGGCCTGCTGGATAAACTGGTCGACCGCGC‑TAACCGGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTc                              <  2:317145/238‑1 (MQ=255)                                                                                                                                                                                                                  ttccccggtttgttgtgtgggggg‑TTATTTCTCCGAACACGAAAACGGTTTCCCGCAAGGGCTGCTAGATAACGTGGCTGACCGCGC‑GAATCTGCCGGGTTCCCTCGGAACCTGCCACGCGTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGt                               <  1:236259/218‑1 (MQ=255)                                                                                                                                                                                                                  tgccccgtttgtttgtggtggggt‑ATATTTCCCGTGTCACGAACACAGCTCCCCGCAAGGGCTGCGGTATAACCGGGTCGAACGCGC‑TATTCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGt                               <  1:474734/218‑1 (MQ=255)                                                                                                                                                                                                                   ctccgttttttgtgtgtgggggg‑ATCTTTTCCCTATCTCGAAATCCGCTTCCCGAAAGGGCGGCGGGATAACGTGGTCAAAC‑CGCGTAATTGGCCGGGTCACCTGGGTAACTGCCACTTTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTc                              <  1:754378/219‑1 (MQ=255)                                                                                                                                                                                                                          ttttttttGGGGGGGG‑TTTTTTCCCCGATCACAAAAAACGCTCCCCGCAAGTGCTGGTGGTAAACCTGGGCGACCGGGC‑AAATCTGCGGTGTTCCCTCGGAACCTGCCCCTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATaa                       <  1:460344/233‑1 (MQ=255)                                                                                                                                                                                                                          ttttGTTTGAGTGGGA‑ATTGTTCCCCGACCACGAAAACAGCTTCCCGCAAGGGCTGCTGGATAACCTGGTCGAACGCGC‑TAATCTGCCGGTTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATaa                       <  2:171693/235‑1 (MQ=255)                                                                                                                                                                                                                          ttCTTTTTGGGTGGGG‑ATTTTTCCCCAACCCAGAAACCGGCTTCCGGCAAGGGCTGCTGGATAAACTGGTCGAACGTGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTTTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATaa                       <  2:498176/238‑1 (MQ=255)                                                                                                                                                                                                                           tttGTTTGAGTGGGG‑AAATTCCTCCGTCCAAGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAAc                     <  2:110275/237‑1 (MQ=255)                                                                                                                                                                                                                              gTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGGGTTCCAGATTGCCCGCCATGAAGAAACTTTCGGTCTGGATAAActca                  >  1:264180/1‑238 (MQ=255)                                                                                                                                                                                                                               tttGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTTTTCCAGATGGCCTGCCATGAGGAAACTTTCGGTCTGGTTAAAccct                  >  2:290191/1‑238 (MQ=255)                                                                                                                                                                                                                                   aGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAGGGGCGTCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGaa             <  2:420300/239‑1 (MQ=255)                                                                                                                                                                                                                                    gTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACTTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAActcatcga              >  2:768136/1‑232 (MQ=255)                                                                                                                                                                                                                                    gTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGa              >  2:4366/1‑237 (MQ=255)                                                                                                                                                                                                                                                tctcCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCCGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCGGATTGCCTGCCATGAAGAAACTTTCGGTCGGGATAAACTCTACGAACTGTGCGAAAt  >  2:174486/1‑238 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             CAAAGGCGAAGCTGATAACGGTCGTAAAGGCCCGCTCAATCTGCCAAATCTGACCCGTCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTAT‑CGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGT‑AAAGATACCCC‑GTCTGGTCACTGGGAAATTGCCGGTGTCCCG‑G‑TTCTGTTTGAGTGGGG‑ATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGC‑TAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAAT  >  NC_000913/4619720‑4620190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑