Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 283,186 | A→G | 18.7% | *303W (TGA→TGG) | yagE → | 2‑keto‑3‑deoxy gluconate (KDG) aldolase, CP4‑6 prophage |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 283,186 | 0 | A | G | 18.7% | 117.3 / 3.1 | 59 | *303W (TGA→TGG) | yagE | 2‑keto‑3‑deoxy gluconate (KDG) aldolase, CP4‑6 prophage |
Reads supporting (aligned to +/- strand): ref base A (20/25); new base G (7/6); total (28/31) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.53e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATCTTCTGAAA‑GCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCG > NC_000913/282950‑283423 | aaTCTT‑TGAAAGGCCGGGCGCGACGGGGACGTGGCAAAAGCGGCCGGGTATCATCAACCCTGGCTGCAAATTCCGCAGATGTATCGGCTGGATACGCCGTTTGTGAACGTGATTAAAGGGGCGACCGGCCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAg < 1:545895/238‑1 (MQ=255) ttcttctAAAG‑CCCTGGGGGGGCGGGGACGGGGGAAAAGGGGCCGGTGAACAGCAGCCCTTGCTGCAATTCCCGGGAGTGTTCCAGCTGGAAAGGCCGTTTGGGAACGTGATTAAAGAGGCGATCGGGCTCTGGGGCCGTCCTGCCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGc < 1:373086/237‑1 (MQ=255) aTCTTCTGAAA‑GCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGCAAACCCTGCTGCAACAGCTCAAGCTTTCCTGAGcc > 2:269195/1‑239 (MQ=255) gAAA‑GCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGAt < 2:456431/239‑1 (MQ=255) cTGGCCGGACGGGGACGGGGCGAAAGCGGCCGGGTAACAACAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGGGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGAt < 2:818493/239‑1 (MQ=255) ggCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCCCGTGCTTCCGCCCGCCTCGCCCCTGGACGCGCCGCGCAAGGCGCAGCTGAAAACCCCCCTCCAAAACCCCAAGCTTTCCCGGGCCGGagagaaccaata > 1:824585/1‑227 (MQ=255) ggggCGAAAGCGGCCGGGTAACATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAAtt < 2:728051/237‑1 (MQ=255) tGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGGGCCGCCCAAGGCGCAGCTGAAAACCCTGCTGCAACGACCCAACCTTTGCTGGCCCGGG‑CGAAAAAGGTGACCATTGAGAAAAatt > 1:871165/1‑239 (MQ=255) gAAACCGCCCGGGTTTCCTCCGACCTTGGAGCAAATCCCGCAAGTGTACTAGCGGGATGCGGCGTTTTGGCACTGAATAAAATGGGCGCTCGGTCCCTGCGGGCGTCCTGTCCCCACGCCCGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCa < 1:149806/238‑1 (MQ=255) aGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCACCAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCAcccc > 2:206619/1‑239 (MQ=255) aagatacagcctcggcccTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAg < 2:123908/223‑1 (MQ=255) tcaGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGACACGGGCAAGGCGCAACTGAAAACCCTGCGGCAACAGCCCACGCTTTGCGGAGCCGGG‑CGGTAACCAGGGCCAATGGGAAAAgttaacccccccacgaccagacgtt > 1:792049/1‑217 (MQ=255) tcaGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCAGCCTCGCCGCTGGACGAGCCGGGCCAGGCGCAGCTGCAAACCCTGCTGCCACAGCTCAAGCTTTGCTGCGCCGGA‑CGACAACGAGGACCATTGAGAAAATTTTTACCCCGCCGGCCGACGCGtt > 1:213854/1‑239 (MQ=255) tcaGACCTTGCTGCAAATTCCGCAGATGTAACAGCTGGATACGCCGTTTGTGAACGTGCTTAAAGAGGTGATCCTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCCCAAGGCGCAGCTGAAAACCCTCCTGCAACCGCTCAAGCTTTGCTGACCCGGA‑CGGTAACGACGACCCTGGAGAAAATTTTCACCCCGccaaaagaacggtt > 1:835677/1‑226 (MQ=255) ccTTGCTGCAAATTCCGCGTGTGTATCAGCGGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGGCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATg < 2:274152/239‑1 (MQ=255) tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTTACCCCGCAGGACGACGCGTTTTATGCgg > 2:43468/1‑239 (MQ=255) tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCCAGCAAGGCGCCGCTGAAAACCCTGCTGCACCAGACCAAGCTTTGCTGGGCCGGA‑CGAAACCGAGGACCAATCAGAAACATTTAACCCCTCAGgacacccgcgtttaggagg > 1:871942/1‑223 (MQ=255) tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGACCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCCGCAACAGCTCACGCTTTGCTGGGCCGGA‑CGATAACCAGTGCCATTTGGAAACTTTTCACCCCGCCGGACGACGGGTTTTTTgggg > 1:788111/1‑239 (MQ=255) gctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAAAGAAGCCCATTGAGAAAATTTTCACCCCGCAGACCGACGCGttctggccctt > 1:123908/1‑230 (MQ=255) aaaTTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTCCTGCACCAGCTCAAGCTTTGCTGGGCCGGC‑CGAAAACGAGGACCATTTAGAAAATTTTCACCCCGCAGGCCGACCCGTTTATTGCGGGGaca > 1:806443/1‑236 (MQ=255) tCCGCAGATGTATCCGCTGGATACGCCGTTTGTGAACGTGATTAAGGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGGGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCa < 2:611659/239‑1 (MQ=255) tGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCCCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGA‑CGATAACCATGACCATTGAGAAAATTTTCACCCCGCAGGCCGACCCGTTTTAGTGGGTAATCACCCACGcgccgg > 1:187751/1‑239 (MQ=255) aTACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGC‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGcggtgc > 2:54336/1‑233 (MQ=255) ccGCCGTTTGTGAACGTGATTAAAGGGGCGATCGTGCCCTGCGGTCGTCCTGTCCCCCCGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGct < 2:20223/238‑1 (MQ=255) ccGCCGTTTGGGAACGTGATTAAAGGGGCGGTCGTGCCCGGCGGGCGCCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGct < 2:422675/238‑1 (MQ=255) tttcaaCGTGTTTAAGAGGGCGATCGTGGTGTGCGGTCGCCCTGTCTCCACGCACGTGCTGCCCCCCGCCTCGCCGCTGGACGGGCCGCGCAAGGCGCCGCTGAAAACCCTGCTGCAACAGCTGAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCt < 1:724221/235‑1 (MQ=255) tGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTACTGGGCCGGC‑CGATAACGATGACCATTGGGAAAATTTTAACCCCGAAGGACGAAGCCGTTTATGCGGGGATACACCACGCGCCGGGGCCGCAGGGCGGTCTGcgcccgacacccg > 1:523504/1‑227 (MQ=255) ggtcgctcttgctcgcgcgccctcCTGTCCCCCCGCACGTGCCGCCCCCCGCCTCCCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGa < 1:655353/217‑1 (MQ=255) gCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCCCTGACCCCCCAAATGCTGTTg > 2:417664/1‑239 (MQ=255) tctGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGATCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCCCCAATGCGCATGGAATCTccc > 2:541280/1‑238 (MQ=255) gtcCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAACATTTTCACCCCGCCGGACGCCGCGTTTTATGCGGGGATCACCCCCGCGGCGGGGCCCCCGGGCGGTCTGCCGCCGACCCCGCAGCGGCtggtgggactcccccgcggccac > 1:444767/1‑221 (MQ=255) ctcctgccccccccccctggctgcGGCCCGCCCCGCCGGTGGACGGGCCGCGCAAGGCGCAGCGTAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGGCCATTGAGAAAATTTTCGCCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAAc < 1:863017/219‑1 (MQ=255) cTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAACATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATCCTGTTGGAACCTCCCGCCGGCAcccgc > 2:241060/1‑236 (MQ=255) ccccgcacgTGCTGCCCCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa < 2:262592/236‑1 (MQ=255) cccccaccgTGCGCCCGCCCGCCCCGCCGCGGGACGGGCCGCGCAAGGGGCAGCTGAAAACCCTGCTGCAAAAGGTCAAGCTTTGCTGGGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa < 1:325323/232‑1 (MQ=255) ccACGTACGTGCTGCCGCCCGGCTCGCCGCTGGACGAGCCGGGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa < 2:142880/239‑1 (MQ=255) ccACGCACGTGCTGCCGCCCGCCTCGCCGGTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa < 2:783647/239‑1 (MQ=255) cgTGCTGCCGCCCGCCTCGCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa < 2:871165/239‑1 (MQ=255) tgctgcCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGa > 2:181916/1‑239 (MQ=255) cccgccTCGCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCggg < 2:806443/239‑1 (MQ=255) ccgccTCGCCGCTGGACGGGCCGCGCAAGGCGAAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGa < 2:824585/239‑1 (MQ=255) ccTGGACGCGCCCCGCAAGGCCCCGGCAGAAACCCTGTCGCAAAAGCTCCAGCTTTGCTGAGGCGGACCGAT‑ACGATGACCTTTGAGAAAATTTGCACCCCGCAGGACGACACGTTTTATGCGGTGATCCCCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGa < 1:379704/238‑1 (MQ=255) atgggagaggcgcggcAGGGCGCCGTGGAAACCCCGGCGGAAACAGCTCAATGTTGGAGGGGCCGGA‑CGATGAGGATGACCATGGAGAAAATTTTCCACCCGCAGGCCGACGCTTTTTATGCGGTGATCACCCCCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCGGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGa < 1:198428/225‑1 (MQ=255) gACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCCCAGGACGCCGCGTTTTATACGGTGATCACCCACGCGGCGGCGCCCCCGGGCGCTCTACACCTGCCCCCGCACACGCTGCTGGAAACTCCCCCACGCAACCGCTTCGCGATGACCCAAAACGCCCGGACGGGCCTGGAcccc > 1:196742/1‑239 (MQ=255) ccacccGCGCAAGGGGCAGCTGAAAACCCTGCTGGAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTAGGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCca < 1:417664/234‑1 (MQ=255) gcAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGA‑CGATAACGATGACCATTGAGAAAAGTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCa < 2:335811/239‑1 (MQ=255) aaGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCAc < 2:404437/238‑1 (MQ=255) cgcAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGC‑CGATAACGTTGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCCGGGCGCCCTGCCGCCTGCCCCGCAGCTGCGGAGGGATCCTCCCACGGCCAACCTGTTCCGCCTGACGCAGACACCGGGGGTTGGCGGGGGCGCcacacaacccccccgca > 1:624936/1‑224 (MQ=255) aaaaCCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAggg > 2:306171/1‑237 (MQ=255) aaaaCCCTGCGTCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTg < 2:871942/239‑1 (MQ=255) tgctgcAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCCGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGTTGGGCTGGGGCGCCAACAAGCCCACCGGCCAAGAGGTGCTGAtt > 2:187602/1‑238 (MQ=255) tgctgcAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGAAGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAAAGGTGCTgct > 2:512278/1‑235 (MQ=255) tgcAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGGCGCGTTTTATGCGGTGAGCACCCACGCGGCGGGGCCGCAGGGCTCTCTGCCGCTGACCCCGCAGATGCTGAGGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCg < 2:213854/239‑1 (MQ=255) aCAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGTCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGGCGCCAACAAGCCCACCGGCAACAGGGTGCTGATTATCGGCAc > 2:537467/1‑239 (MQ=255) cAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTTACCCCGCAACTGCTGATGGACTCTCCCAGCGGCAACCCGTTCGGCATGACGCAGACCCCCGGGATTGGCCGGGACGCCAACAAGCCCCCCGCCAAAAAGGCGCTGGTTATGGGCAc > 1:184335/1‑238 (MQ=255) cgCTCAAGCTTTGCTGGGGCGGA‑CGATAACGATGACCTTTGAGAAAATTTTCCCCCCGCAGGAGGGCGCGTTTTATGCGGTGATCCCCCACGGGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc < 1:886182/238‑1 (MQ=255) aGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc < 2:523504/239‑1 (MQ=255) cAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGGGCTGGTTATCGGGCCTCAggg > 2:751503/1‑239 (MQ=255) gAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCcgcg < 2:456582/239‑1 (MQ=255) | AATCTTCTGAAA‑GCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGA‑CGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCG > NC_000913/282950‑283423 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |