Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 283,186 A→G 18.7% *303W (TGA→TGG yagE → 2‑keto‑3‑deoxy gluconate (KDG) aldolase, CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913283,1860AG18.7% 117.3 / 3.1 59*303W (TGA→TGGyagE2‑keto‑3‑deoxy gluconate (KDG) aldolase, CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base G (7/6);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCG  >  NC_000913/282950‑283423
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
aaTCTTTGAAAGGCCGGGCGCGACGGGGACGTGGCAAAAGCGGCCGGGTATCATCAACCCTGGCTGCAAATTCCGCAGATGTATCGGCTGGATACGCCGTTTGTGAACGTGATTAAAGGGGCGACCGGCCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAg                                                                                                                                                                                                                                               <  1:545895/238‑1 (MQ=255)
 ttcttctAAAGCCCTGGGGGGGCGGGGACGGGGGAAAAGGGGCCGGTGAACAGCAGCCCTTGCTGCAATTCCCGGGAGTGTTCCAGCTGGAAAGGCCGTTTGGGAACGTGATTAAAGAGGCGATCGGGCTCTGGGGCCGTCCTGCCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGc                                                                                                                                                                                                                                              <  1:373086/237‑1 (MQ=255)
 aTCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGCAAACCCTGCTGCAACAGCTCAAGCTTTCCTGAGcc                                                                                                                                                                                                                                             >  2:269195/1‑239 (MQ=255)
        gAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGAt                                                                                                                                                                                                                                     <  2:456431/239‑1 (MQ=255)
               cTGGCCGGACGGGGACGGGGCGAAAGCGGCCGGGTAACAACAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGGGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGAt                                                                                                                                                                                                                               <  2:818493/239‑1 (MQ=255)
                 ggCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCCCGTGCTTCCGCCCGCCTCGCCCCTGGACGCGCCGCGCAAGGCGCAGCTGAAAACCCCCCTCCAAAACCCCAAGCTTTCCCGGGCCGGagagaaccaata                                                                                                                                                                                                                               >  1:824585/1‑227 (MQ=255)
                               ggggCGAAAGCGGCCGGGTAACATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAAtt                                                                                                                                                                                                               <  2:728051/237‑1 (MQ=255)
                                tGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGGGCCGCCCAAGGCGCAGCTGAAAACCCTGCTGCAACGACCCAACCTTTGCTGGCCCGGGCGAAAAAGGTGACCATTGAGAAAAatt                                                                                                                                                                                                              >  1:871165/1‑239 (MQ=255)
                                    gAAACCGCCCGGGTTTCCTCCGACCTTGGAGCAAATCCCGCAAGTGTACTAGCGGGATGCGGCGTTTTGGCACTGAATAAAATGGGCGCTCGGTCCCTGCGGGCGTCCTGTCCCCACGCCCGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCa                                                                                                                                                                                                           <  1:149806/238‑1 (MQ=255)
                                       aGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCACCAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCAcccc                                                                                                                                                                                                       >  2:206619/1‑239 (MQ=255)
                                           aagatacagcctcggcccTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAg                                                                                                                                                                                                   <  2:123908/223‑1 (MQ=255)
                                                      tcaGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGACACGGGCAAGGCGCAACTGAAAACCCTGCGGCAACAGCCCACGCTTTGCGGAGCCGGGCGGTAACCAGGGCCAATGGGAAAAgttaacccccccacgaccagacgtt                                                                                                                                                                                        >  1:792049/1‑217 (MQ=255)
                                                      tcaGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCAGCCTCGCCGCTGGACGAGCCGGGCCAGGCGCAGCTGCAAACCCTGCTGCCACAGCTCAAGCTTTGCTGCGCCGGACGACAACGAGGACCATTGAGAAAATTTTTACCCCGCCGGCCGACGCGtt                                                                                                                                                                                        >  1:213854/1‑239 (MQ=255)
                                                      tcaGACCTTGCTGCAAATTCCGCAGATGTAACAGCTGGATACGCCGTTTGTGAACGTGCTTAAAGAGGTGATCCTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCCCAAGGCGCAGCTGAAAACCCTCCTGCAACCGCTCAAGCTTTGCTGACCCGGACGGTAACGACGACCCTGGAGAAAATTTTCACCCCGccaaaagaacggtt                                                                                                                                                                                        >  1:835677/1‑226 (MQ=255)
                                                           ccTTGCTGCAAATTCCGCGTGTGTATCAGCGGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGGCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATg                                                                                                                                                                                   <  2:274152/239‑1 (MQ=255)
                                                              tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTTACCCCGCAGGACGACGCGTTTTATGCgg                                                                                                                                                                                >  2:43468/1‑239 (MQ=255)
                                                              tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCCAGCAAGGCGCCGCTGAAAACCCTGCTGCACCAGACCAAGCTTTGCTGGGCCGGACGAAACCGAGGACCAATCAGAAACATTTAACCCCTCAGgacacccgcgtttaggagg                                                                                                                                                                                >  1:871942/1‑223 (MQ=255)
                                                              tgctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGACCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCCGCAACAGCTCACGCTTTGCTGGGCCGGACGATAACCAGTGCCATTTGGAAACTTTTCACCCCGCCGGACGACGGGTTTTTTgggg                                                                                                                                                                                >  1:788111/1‑239 (MQ=255)
                                                               gctgcAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAAAGAAGCCCATTGAGAAAATTTTCACCCCGCAGACCGACGCGttctggccctt                                                                                                                                                                               >  1:123908/1‑230 (MQ=255)
                                                                    aaaTTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTCCTGCACCAGCTCAAGCTTTGCTGGGCCGGCCGAAAACGAGGACCATTTAGAAAATTTTCACCCCGCAGGCCGACCCGTTTATTGCGGGGaca                                                                                                                                                                           >  1:806443/1‑236 (MQ=255)
                                                                        tCCGCAGATGTATCCGCTGGATACGCCGTTTGTGAACGTGATTAAGGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGGGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCa                                                                                                                                                                      <  2:611659/239‑1 (MQ=255)
                                                                                tGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCCCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGACGATAACCATGACCATTGAGAAAATTTTCACCCCGCAGGCCGACCCGTTTTAGTGGGTAATCACCCACGcgccgg                                                                                                                                                              >  1:187751/1‑239 (MQ=255)
                                                                                            aTACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGCCGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGcggtgc                                                                                                                                                   >  2:54336/1‑233 (MQ=255)
                                                                                              ccGCCGTTTGTGAACGTGATTAAAGGGGCGATCGTGCCCTGCGGTCGTCCTGTCCCCCCGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGct                                                                                                                                                <  2:20223/238‑1 (MQ=255)
                                                                                              ccGCCGTTTGGGAACGTGATTAAAGGGGCGGTCGTGCCCGGCGGGCGCCCTGTCCCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGct                                                                                                                                                <  2:422675/238‑1 (MQ=255)
                                                                                                      tttcaaCGTGTTTAAGAGGGCGATCGTGGTGTGCGGTCGCCCTGTCTCCACGCACGTGCTGCCCCCCGCCTCGCCGCTGGACGGGCCGCGCAAGGCGCCGCTGAAAACCCTGCTGCAACAGCTGAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCt                                                                                                                                        <  1:724221/235‑1 (MQ=255)
                                                                                                              tGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTACTGGGCCGGCCGATAACGATGACCATTGGGAAAATTTTAACCCCGAAGGACGAAGCCGTTTATGCGGGGATACACCACGCGCCGGGGCCGCAGGGCGGTCTGcgcccgacacccg                                                                                                                                >  1:523504/1‑227 (MQ=255)
                                                                                                                       ggtcgctcttgctcgcgcgccctcCTGTCCCCCCGCACGTGCCGCCCCCCGCCTCCCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGa                                                                                                                       <  1:655353/217‑1 (MQ=255)
                                                                                                                         gCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCCCTGACCCCCCAAATGCTGTTg                                                                                                                     >  2:417664/1‑239 (MQ=255)
                                                                                                                                   tctGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGATCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCCCCAATGCGCATGGAATCTccc                                                                                                            >  2:541280/1‑238 (MQ=255)
                                                                                                                                            gtcCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAACATTTTCACCCCGCCGGACGCCGCGTTTTATGCGGGGATCACCCCCGCGGCGGGGCCCCCGGGCGGTCTGCCGCCGACCCCGCAGCGGCtggtgggactcccccgcggccac                                                                                                   >  1:444767/1‑221 (MQ=255)
                                                                                                                                            ctcctgccccccccccctggctgcGGCCCGCCCCGCCGGTGGACGGGCCGCGCAAGGCGCAGCGTAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGGCCATTGAGAAAATTTTCGCCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAAc                                                                                                   <  1:863017/219‑1 (MQ=255)
                                                                                                                                               cTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAACATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATCCTGTTGGAACCTCCCGCCGGCAcccgc                                                                                                >  2:241060/1‑236 (MQ=255)
                                                                                                                                                     ccccgcacgTGCTGCCCCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa                                                                                         <  2:262592/236‑1 (MQ=255)
                                                                                                                                                     cccccaccgTGCGCCCGCCCGCCCCGCCGCGGGACGGGCCGCGCAAGGGGCAGCTGAAAACCCTGCTGCAAAAGGTCAAGCTTTGCTGGGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa                                                                                         <  1:325323/232‑1 (MQ=255)
                                                                                                                                                     ccACGTACGTGCTGCCGCCCGGCTCGCCGCTGGACGAGCCGGGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa                                                                                         <  2:142880/239‑1 (MQ=255)
                                                                                                                                                     ccACGCACGTGCTGCCGCCCGCCTCGCCGGTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCa                                                                                         <  2:783647/239‑1 (MQ=255)
                                                                                                                                                            cgTGCTGCCGCCCGCCTCGCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCa                                                                                  <  2:871165/239‑1 (MQ=255)
                                                                                                                                                              tgctgcCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGa                                                                                >  2:181916/1‑239 (MQ=255)
                                                                                                                                                                      cccgccTCGCCGCTGGACGGGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCggg                                                                        <  2:806443/239‑1 (MQ=255)
                                                                                                                                                                       ccgccTCGCCGCTGGACGGGCCGCGCAAGGCGAAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGa                                                                       <  2:824585/239‑1 (MQ=255)
                                                                                                                                                                                 ccTGGACGCGCCCCGCAAGGCCCCGGCAGAAACCCTGTCGCAAAAGCTCCAGCTTTGCTGAGGCGGACCGATACGATGACCTTTGAGAAAATTTGCACCCCGCAGGACGACACGTTTTATGCGGTGATCCCCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGa                                                             <  1:379704/238‑1 (MQ=255)
                                                                                                                                                                                 atgggagaggcgcggcAGGGCGCCGTGGAAACCCCGGCGGAAACAGCTCAATGTTGGAGGGGCCGGACGATGAGGATGACCATGGAGAAAATTTTCCACCCGCAGGCCGACGCTTTTTATGCGGTGATCACCCCCGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCGGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGa                                                             <  1:198428/225‑1 (MQ=255)
                                                                                                                                                                                     gACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCCCAGGACGCCGCGTTTTATACGGTGATCACCCACGCGGCGGCGCCCCCGGGCGCTCTACACCTGCCCCCGCACACGCTGCTGGAAACTCCCCCACGCAACCGCTTCGCGATGACCCAAAACGCCCGGACGGGCCTGGAcccc                                                         >  1:196742/1‑239 (MQ=255)
                                                                                                                                                                                       ccacccGCGCAAGGGGCAGCTGAAAACCCTGCTGGAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTAGGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCca                                                        <  1:417664/234‑1 (MQ=255)
                                                                                                                                                                                               gcAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGACGATAACGATGACCATTGAGAAAAGTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCa                                               <  2:335811/239‑1 (MQ=255)
                                                                                                                                                                                                 aaGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGGGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCAc                                              <  2:404437/238‑1 (MQ=255)
                                                                                                                                                                                                     cgcAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGCCGATAACGTTGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCCGGGCGCCCTGCCGCCTGCCCCGCAGCTGCGGAGGGATCCTCCCACGGCCAACCTGTTCCGCCTGACGCAGACACCGGGGGTTGGCGGGGGCGCcacacaacccccccgca                                         >  1:624936/1‑224 (MQ=255)
                                                                                                                                                                                                             aaaaCCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAggg                                  >  2:306171/1‑237 (MQ=255)
                                                                                                                                                                                                             aaaaCCCTGCGTCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTg                                 <  2:871942/239‑1 (MQ=255)
                                                                                                                                                                                                                    tgctgcAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCCGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGTTGGGCTGGGGCGCCAACAAGCCCACCGGCCAAGAGGTGCTGAtt                           >  2:187602/1‑238 (MQ=255)
                                                                                                                                                                                                                    tgctgcAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGAAGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAAAGGTGCTgct                            >  2:512278/1‑235 (MQ=255)
                                                                                                                                                                                                                       tgcAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGGCGCGTTTTATGCGGTGAGCACCCACGCGGCGGGGCCGCAGGGCTCTCTGCCGCTGACCCCGCAGATGCTGAGGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCg                       <  2:213854/239‑1 (MQ=255)
                                                                                                                                                                                                                           aCAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGTCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGGCGCCAACAAGCCCACCGGCAACAGGGTGCTGATTATCGGCAc                   >  2:537467/1‑239 (MQ=255)
                                                                                                                                                                                                                            cAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTTACCCCGCAACTGCTGATGGACTCTCCCAGCGGCAACCCGTTCGGCATGACGCAGACCCCCGGGATTGGCCGGGACGCCAACAAGCCCCCCGCCAAAAAGGCGCTGGTTATGGGCAc                   >  1:184335/1‑238 (MQ=255)
                                                                                                                                                                                                                             cgCTCAAGCTTTGCTGGGGCGGACGATAACGATGACCTTTGAGAAAATTTTCCCCCCGCAGGAGGGCGCGTTTTATGCGGTGATCCCCCACGGGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc                 <  1:886182/238‑1 (MQ=255)
                                                                                                                                                                                                                             aGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTc                 <  2:523504/239‑1 (MQ=255)
                                                                                                                                                                                                                                 cAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGGGCTGGTTATCGGGCCTCAggg             >  2:751503/1‑239 (MQ=255)
                                                                                                                                                                                                                                            gAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCcgcg  <  2:456582/239‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
AATCTTCTGAAAGCCTGGCGCGACGGGGACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAGCTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTCTCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAAACCCTGCTGCAACAGCTCAAGCTTTGCTGAGCCGGACGATAACGATGACCATTGAGAAAATTTTCACCCCGCAGGACGACGCGTTTTATGCGGTGATCACCCACGCGGCGGGGCCGCAGGGCGCTCTGCCGCTGACCCCGCAGATGCTGATGGAATCTCCCAGCGGCAACCTGTTCGGCATGACGCAGAACGCCGGGATGGGCTGGGACGCCAACAAGCTCACCGGCAAAGAGGTGCTGATTATCGGCACTCAGGGCGGCATCCGCG  >  NC_000913/282950‑283423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: