Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 33,456 C→A 21.8% T880T (ACC→ACA carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091333,4560CA21.8% 96.0 / 4.2 52T880T (ACC→ACAcarBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (30/7);  new base A (3/9);  total (33/19)
Fisher's exact test for biased strand distribution p-value = 7.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGG  >  NC_000913/33223‑33691
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            
cAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCCCGCGTGATGGGTGGCAAATCGCTGGCTGAGCAGGGCGTAACCCaaga                                                                                                                                                                                                                                         >  2:862861/1‑239 (MQ=255)
cAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGGTCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCaaa                                                                                                                                                                                                                                           <  2:43631/237‑1 (MQ=255)
   gAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCCGGGGGAGGCTGGCAAAACGCCGGCAGAGCAAGGGGTAACAaaaaaaag                                                                                                                                                                                                                                      >  1:206164/1‑237 (MQ=255)
           ggATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGCCGTACCGCTGGCAAAAGTGGCGGGGCGCGGGCTGGCTGGCAAATCGCTGGCGGCGCAAGGCGGAACCAAAGAAGTTATCccg                                                                                                                                                                                                                               >  2:855784/1‑238 (MQ=255)
              tgtgATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCGGGCAAAACCCTGGCGGAGCAGGGGGGAACAAAAAAAAGTAAAccgccgc                                                                                                                                                                                                                           >  1:300074/1‑238 (MQ=255)
                          gcagGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGCGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCgggtg                                                                                                                                                                                                               >  2:324369/1‑236 (MQ=255)
                          gcagGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCCCGCGTGATGGCTGGCAAATCCCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACCACTCGGGGa                                                                                                                                                                                                               >  2:499504/1‑239 (MQ=255)
                                         ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGGGCGTGATGGGTGGCAAAACGCTGGCTGAGCAAGGGGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGGCAGAAGGGGggctgg                                                                                                                                                                                                >  2:382151/1‑238 (MQ=255)
                                         ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGGGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTAGggctg                                                                                                                                                                                                 >  2:321319/1‑238 (MQ=255)
                                         ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAAGGCGTAACCAAAGAAGTTATCCCGCCGTCCTACTCTGTGAAAGAAGTGAggctgc                                                                                                                                                                                                >  2:145184/1‑239 (MQ=255)
                                          gCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCGGAGCAGGGCGGAACCAAAGAAGGTTTCCCGCCGGACGACTCGGGGCAAAAAGGGGggcgtcc                                                                                                                                                                                               >  2:372836/1‑235 (MQ=255)
                                             ctcgactggccgggccggggCCTGATGAAGTTGCTGTTTGAGGGGAAAAAAAACGAAGTCTCCCTGATTGAATGTCGCCCGCGTGCGGCGAGTCCCTTTCCGTTCGTCTCAAAAGCCACCGGCGTACCGCTGGAAAAGGGGCGGGCGCGCGTGTTGGCGGGAAAATCGTGGGTTGAGCAGGGCGTACCCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGtt                                                                                                                                                                                            <  1:862861/221‑1 (MQ=255)
                                              tCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTTTCCCGCCGTACTACTCGGGGAAAGGAGTGGGGCTGCCGTTc                                                                                                                                                                                           >  2:357363/1‑239 (MQ=255)
                                               cGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCCCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGAGGTGCTGCCGTTCa                                                                                                                                                                                          >  2:198965/1‑239 (MQ=255)
                                                            cgcgGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCCAATCGCTGGCTTGGCAGGGCGGAACCAAAGAAGTTATCCCGCCGAACCACTCGGTGAAAGAAGAGGTGGTGCCGTTCATATAATTccccgc                                                                                                                                                                             >  2:871205/1‑236 (MQ=255)
                                                              cgGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGAAAGAAGGGGGGCCGCCGGTCACTAAATTCCCGGGCg                                                                                                                                                                           >  2:673964/1‑239 (MQ=255)
                                                                         aCGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGGAGTGCGGCCGTTCAATAAATTCCCGGGCGTTGAccc                                                                                                                                                                    >  2:853916/1‑235 (MQ=255)
                                                                           gcgCAGATTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCAGTTCGTCTCCAAAGCCACAGGCGTACCGCTGGCATAAGTGGCGGCGCGCGTGGCGGCTGGCAAATCGCTGGCTGGCCCGGCCGGAACAAAAGCGTGTATACCGCCGTAGAATACGGGGAAAGGAGCGGGCGTGCCGTTCCAAAAAATCCAGGGCGTTGACCAGCGGtt                                                                                                                                                              >  1:329921/3‑239 (MQ=255)
                                                                           gTGAAGTTTGCGGTAAAAAACAACGAAGCCTACCTGATTGAAGTTACCCCGCGTGCGGCGCGTCCCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGtt                                                                                                                                                              <  2:297693/239‑1 (MQ=255)
                                                                                  ttGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGGACTACTCGGTGGAAGAAGGGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCCGTTAGGGCCa                                                                                                                                                       >  2:264641/1‑239 (MQ=255)
                                                                                      ggggaaaacacgcgattcctcccTGTTTGGAGTTCCCCCGCGCGCGGCGCGTACCTTTCCGTCCGTCTCCAAAGCCACGGGCTTCCCGCTGGAAAAGGTGGCGCCGCGCGTGATGGCTGGAAAACACTGGGTTGAGAGGGGCGTAACAAAAGAGTTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGaa                                                                                                                                                    <  1:347615/217‑1 (MQ=255)
                                                                                             accacggcagcttACCTGTTGAAGTGCACGCGCTGTGCGGCGCTCGCCGTGCCGTTCGACCGCACAGCCCCCGGCGTCCGGCGGGCAAGGGGGGCGGCGCGGGGGATGGTGGTCAAGTCGCGGGGTGGGAAGGGCGTAACAAAAGAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGctc                                                                                                                                           <  1:871205/227‑1 (MQ=255)
                                                                                                       tttcctgtttggAAGTGACCCCGGGGGGGCCGCGGCCCGTCCCGTCCGTCTCAAAACCCACGGGGTGACCGGGGGCAAAAGTGGGGGCGCGGGGGATGGCTGGAAATTCGCGTGCTGAGCGGGGCGAACCAAAAGAATTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCggg                                                                                                                                    <  1:324369/225‑1 (MQ=255)
                                                                                                          aCCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCCTACTACTCGGGGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGGTGACCCCCTGTTTGGGCCCGAAATGCGGCCTACCGGGGAAggc                                                                                                                              >  2:415044/1‑237 (MQ=255)
                                                                                                                 tGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGAAAGAAGTGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCGGAAATGCGCTCTACCGGGGAAGTCATGGGCg                                                                                                                       >  2:641651/1‑239 (MQ=255)
                                                                                                                     gTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGGCCCCCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGgggg                                                                                                                   >  2:396347/1‑239 (MQ=255)
                                                                                                                              cgTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGGGCAGGGCGTGACCAAAGAAGTTATTCCGCCGTACTACTCGGGGAAAGAAAGGGTGCTGCCGTTCCATAAATTCCCGGGCGTTTACCCCGCGTTAGGGCCCGAAAGGCGCTCAACAGGGGCAGACCAGGGGGGGGGCCGccccc                                                                                                           >  1:296467/1‑237 (MQ=255)
                                                                                                                              cgTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGGAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTCCCGGGGAAGTCACGGGCGGGGGCCGCCCCt                                                                                                           >  2:128301/1‑238 (MQ=255)
                                                                                                                                                tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGGGCAGGGCGTAACCAAAGAAGGTATCCCGCCGTACTACTCGGGGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGGTAGGGGCCGAAATGCGGCCCACCGGGGAAGGCATGGGGGGGGGCCCCCCCCCCGCCAAAACGTTTGCCa                                                                                         >  1:100163/1‑239 (MQ=255)
                                                                                                                                                tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAAAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGCAATGCGCTCTACCGCGGAATGCATGGGCGTAGGCCGCACCCTCGCTGAAGCGTTTGcgc                                                                                         >  2:322497/1‑237 (MQ=255)
                                                                                                                                                tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGGAACCAAAGAAGTTATCCCGCCCTACTACTCCGTGAAAGAAGTGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGGTCGTGCCCGGAATGCGCTTCAACCGGGGAGTCACGGGCGCGGGCCGCCACCTCCCTGAAACGTTTCCCa                                                                                         >  1:798045/1‑239 (MQ=255)
                                                                                                                                                   ttccGTCCCCAAAGCCCCCGGCGTCCCGCTGGCAAAGGGGGGGGCGGGTGGGATGGTGGGAAAGTCGGTGGCGGGGCAGGGCGTAACCAAAGAAGTTATCCCGCTGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAg                                                                                      <  1:396347/236‑1 (MQ=255)
                                                                                                                                                       gTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGGCCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGACGCGTTTGCCAACGCGCa                                                                                  >  2:204340/1‑239 (MQ=255)
                                                                                                                                                                   aCCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGCAGGCATGGACGGGGGCGGCACCTTCTCTGAAGCGTTTGCCAAAGCACACACGGGCAGCaa                                                                      >  1:78737/1‑239 (MQ=255)
                                                                                                                                                                      ggCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGGCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCaaat                                                                    >  2:614471/1‑236 (MQ=255)
                                                                                                                                                                           cccGCTTGAAAAGGGGGCGGAGCGCGTGAGGGCGGCCAATTGGTGGGCGGGGCAGGGGTTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTccacc                                                               <  2:834302/237‑1 (MQ=255)
                                                                                                                                                                           aCCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGGCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGACGCTGGGCAGCAACTccacca                                                              >  2:278319/1‑239 (MQ=255)
                                                                                                                                                                              ggtGGCAAAAGGGGCGGCGCGCTGGATGTCTGGAAAATCGCGGGCGGAGCAGGGCGTACCAAAAGAAGTTAGCCCGCCGTACTACTCGGGGAAAGAAGTGGTGCTGGCGGTCAATAAATTCCCGGGCGGTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATga                                                           <  2:425076/237‑1 (MQ=255)
                                                                                                                                                                              gcgtgaaaaaGCGGGGGGGCGGGTGGGTGCCGGCAACTGGCTGGTGGAGGCGGGCGTAACAAAAAAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCATAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATga                                                           <  2:737974/233‑1 (MQ=255)
                                                                                                                                                                                 ggCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATgcaga                                                        >  2:307511/1‑239 (MQ=255)
                                                                                                                                                                                      aaggtgggcgccggcgTGATGGCTGACAAACCGCTGGCGAAGCGGGGCGTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAaca                                                    <  2:608654/226‑1 (MQ=255)
                                                                                                                                                                                                          ggctggaAAGTCGCTGGCTGAGCAGGGCTTACCAAAAGAGGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCg                                <  2:300074/239‑1 (MQ=255)
                                                                                                                                                                                                               gcAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGCGGAAGTCATGGGCGTGGGCCGCACCTTCCCTGCAGCGTTTGCCAAAGCGCAACCGGGCCGAAACCCCACCCTGAAGAAACACGGGCGGGCACGGCTTACCGTgcgg                           >  1:491140/1‑238 (MQ=255)
                                                                                                                                                                                                                  aagtggtgggcgggggggggcaaaaCAAAAGATGTTCCCCCGCCGTAGTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGa                         <  1:499504/216‑1 (MQ=255)
                                                                                                                                                                                                                  aacggcgcgggggAGCAGGGCGACACGAAAGAATTTGACCCGCCGTACTACCGGGTGAAAGAAGGGGGTCCGCCGTTCAATAAATTCCCGGGCGGTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGGCATGGGCGGGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGa                         <  1:415044/227‑1 (MQ=255)
                                                                                                                                                                                                                  aaagcgtgggcggggCAGAGCGAACCAAAATAAGCTACCCCGCCGTTCTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGcgcg                          <  2:329921/224‑1 (MQ=255)
                                                                                                                                                                                                                   aTCGCTGGTGGAGCAGGGCGTAACAAAAGAGGTGCGCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAg                       <  2:692457/239‑1 (MQ=255)
                                                                                                                                                                                                                       ctgtgctgAGCGGGGATTACCTAAGAAATTTACCCCTCCGTACTACTCGGGGAAAGAAGTGGTGCTGCCTGTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTTGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCg                    <  2:558312/235‑1 (MQ=255)
                                                                                                                                                                                                                            tgggggggggCGTAACCAAGAAATTTCCCCCGCGGTTCTCTGCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGCCCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATaa                <  1:452292/231‑1 (MQ=255)
                                                                                                                                                                                                                              aggcgtggCGTAACGAAAGAAGTTCTCCCGCCGTACTGCTCGGTTAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAg              <  2:590583/232‑1 (MQ=255)
                                                                                                                                                                                                                                      cGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCgtgg     >  2:232493/1‑239 (MQ=255)
                                                                                                                                                                                                                                         aaCCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCCGCTTTCCGTGGCCGAAGGCGATAAAAAAcggggggggg  >  1:881413/1‑231 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                            
CAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGCGTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGCGGCGCGCGTGATGGCTGGCAAATCGCTGGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGCTCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGG  >  NC_000913/33223‑33691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: