Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 33,456 | C→A | 21.8% | T880T (ACC→ACA) | carB → | carbamoyl‑phosphate synthase large subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 33,456 | 0 | C | A | 21.8% | 96.0 / 4.2 | 52 | T880T (ACC→ACA) | carB | carbamoyl‑phosphate synthase large subunit |
Reads supporting (aligned to +/- strand): ref base C (30/7); new base A (3/9); total (33/19) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.24e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGG > NC_000913/33223‑33691 | cAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCCCGCGTGATGGGTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCCaaga > 2:862861/1‑239 (MQ=255) cAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGGTCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCaaa < 2:43631/237‑1 (MQ=255) gAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCCGGGGGAGGCTGGCAAAACGCC‑GGCAGAGCAAGGGGTAACAaaaaaaag > 1:206164/1‑237 (MQ=255) ggATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGCCGTACCGCTGGCAAAAGTGGC‑GGGGCGCGGGCTGGCTGGCAAATCGCT‑GGCGGCGCAAGGCGGAACCAAAGAAGTTATCccg > 2:855784/1‑238 (MQ=255) tgtgATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCGGGCAAAACCCT‑GGCGGAGCAGGGGGGAACAAAAAAAAGTAAAccgccgc > 1:300074/1‑238 (MQ=255) gcagGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGCGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCgggtg > 2:324369/1‑236 (MQ=255) gcagGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCCCGCGTGATGGCTGGCAAATCCCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACCACTCGGGGa > 2:499504/1‑239 (MQ=255) ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGGGCGTGATGGGTGGCAAAACGCT‑GGCTGAGCAAGGGGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGGCAGAAGGGGggctgg > 2:382151/1‑238 (MQ=255) ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGGGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTAGggctg > 2:321319/1‑238 (MQ=255) ggCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAAGGCGTAACCAAAGAAGTTATCCCGCCGTCCTACTCTGTGAAAGAAGTGAggctgc > 2:145184/1‑239 (MQ=255) gCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCGGAGCAGGGCGGAACCAAAGAAGGTTTCCCGCCGGACGACTCGGGGCAAAAAGGGGggcgtcc > 2:372836/1‑235 (MQ=255) ctcgactggccgggccggggCCTGATGAAGTTGCTGTTTGAGGGGAAAAAAAACGAAGTCTCCCTGATTGAATGTCGCCCGC‑GTGCGGCGAGTCCCTTTCCGTTCGTCTCAAAAGCCACCGGCGTACCGCTGG‑AAAAGGGGCGGGCGCGCGTGTTGGCGGGAAAATCGTG‑GGTTGAGCAGGGCGTACCCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGtt < 1:862861/221‑1 (MQ=255) tCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTTTCCCGCCGTACTACTCGGGGAAAGGAGTGGGGCTGCCGTTc > 2:357363/1‑239 (MQ=255) cGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCCCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGAGGTGCTGCCGTTCa > 2:198965/1‑239 (MQ=255) cgcgGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCCAATCGCT‑GGCTTGGCAGGGCGGAACCAAAGAAGTTATCCCGCCGAACCACTCGGTGAAAGAAGAGGTGGTGCCGTTCATATAATTccccgc > 2:871205/1‑236 (MQ=255) cgGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGAAAGAAGGGGGGCCGCCGGTCACTAAATTCCCGGGCg > 2:673964/1‑239 (MQ=255) aCGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGGAGTGCGGCCGTTCAATAAATTCCCGGGCGTTGAccc > 2:853916/1‑235 (MQ=255) gcgCAGATTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCAGTTCGTCTCCAAAGCCACAGGCGTACCGCTGGCATAAGTGGC‑GGCGCGCGTGGCGGCTGGCAAATCGCT‑GGCTGGCCCGGCCGGAACAAAAGCGTGTATACCGCCGTAGAATACGGGGAAAGGAGCGGGCGTGCCGTTCCAAAAAATCCAGGGCGTTGACCAGCGGtt > 1:329921/3‑239 (MQ=255) gTGAAGTTTGCGGTAAAAAACAACGAAGCCTACCTGATTGAAGTTACCCCGC‑GTGCGGCGCGTCCCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGtt < 2:297693/239‑1 (MQ=255) ttGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGGACTACTCGGTGGAAGAAGGGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCCGTTAGGGCCa > 2:264641/1‑239 (MQ=255) ggggaaaacacgcgattcctcccTGTTTGGAGTTCCCCCGC‑GCGCGGCGCGTACCTTTCCGTCCGTCTCCAAAGCCACGGGCTTCCCGCTGGAAAAGGTGGC‑GCCGCGCGTGATGGCTGG‑AAAACACTGGGTTGAGAGGGGCGTAACAAAAGAGTTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGaa < 1:347615/217‑1 (MQ=255) accacggcagcttACCTG‑TTGAAGTGCACGCGCTGTGCGGCGCTCGCCGTGCCGTTCGACCGCACAGCCCCCGGCGTCCGGCGGGCAAGGGGGGC‑GGCGCGGGGGATGGTGGTCAAGTCGCG‑GGGTGGGAAGGGCGTAACAAAAGAATTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGc‑tc < 1:871205/227‑1 (MQ=255) tttcctgtttggAAGTGACCCCGG‑GGGGGCCGCGGCCCGTCCCGTCCGTCTCAAAACCCACGGGGTGACCGGGGGCAAAAGTGGG‑GGCGCGGGGGATGGCTGGAAATTCGCG‑TGCTGAGCGGGGCGAACCAAAAGAATTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCggg < 1:324369/225‑1 (MQ=255) aCCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCCTACTACTCGGGGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGGTGACCCCCTGTTTGGGCCCGAAATGCGG‑CCTACCGGGGAAggc > 2:415044/1‑237 (MQ=255) tGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGGGAAAGAAGTGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCGGAAATGCGC‑TCTACCGGGGAAGTCATGGGCg > 2:641651/1‑239 (MQ=255) gTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGGCCCCCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGgggg > 2:396347/1‑239 (MQ=255) c‑gTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGGGCAGGGCGTGACCAAAGAAGTTATTCCGCCGTACTACTCGGGGAAAGAAAGGGTGCTGCCGTTCCATAAATTCCCGGGCGTTTACCCCGCGTTAGGGCCCGAAAGGCGC‑TCAACAGGGGCAGACCAGGGGGGGGGCCGccccc > 1:296467/1‑237 (MQ=255) c‑gTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGGAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTCCCGGGGAAGTCACGGGCGGGGGCCGCCCCt > 2:128301/1‑238 (MQ=255) tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGGGCAGGGCGTAACCAAAGAAGGTATCCCGCCGTACTACTCGGGGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGGTAGGGGCCGAAATGCGG‑CCCACCGGGGAAGGCATGGGGGGGGGCCCCCCCCCCGCCAAAACGTTTGCCa > 1:100163/1‑239 (MQ=255) tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAAAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGCAATGCGC‑TCTACCGCGGAATGCATGGGCGTAGGCCGCACCCTCGCTGAAGCGTTTGcgc > 2:322497/1‑237 (MQ=255) tccgttcGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGGAACCAAAGAAGTTATCCCGCCCTACTACTCCGTGAAAGAAGTGGGGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGGTCGTGCCCGGAATGCGCTTCAACCGGGG‑AGTCACGGGCGCGGGCCGCCACCTCCCTGAAACGTTTCCCa > 1:798045/1‑239 (MQ=255) ttccGTCCCCAAAGCCCCCGGCGTCCCGCTGGCAAAGGGGGG‑GGCGGGTGGGATGGTGGGAAAGTCGGT‑GGCGGGGCAGGGCGTAACCAAAGAAGTTATCCCGCTGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAg < 1:396347/236‑1 (MQ=255) gTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGGCCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGACGCGTTTGCCAACGCGCa > 2:204340/1‑239 (MQ=255) aCCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGCAGGCATGGACGGGGGCGGCACCTTCTCTGAAGCGTTTGCCAAAGCACACACGGGCAGCaa > 1:78737/1‑239 (MQ=255) ggCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGGCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCaaat > 2:614471/1‑236 (MQ=255) cccGCTTGAAAAGGGGGC‑GGAGCGCGTGAGGGCGGCCAATTGGTG‑GGCGGGGCAGGGGTTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTccacc < 2:834302/237‑1 (MQ=255) aCCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGGCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGACGCTGGGCAGCAACTccacca > 2:278319/1‑239 (MQ=255) ggtGGCAAAAGGGGC‑GGCGCGCTGGATGTCTGGAAAATCGCG‑GGCGGAGCAGGGCGTACCAAAAGAAGTTAGCCCGCCGTACTACTCGGGGAAAGAAGTGGTGCTGGCGGTCAATAAATTCCCGGGCGGTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATga < 2:425076/237‑1 (MQ=255) gcgtgaaaaaGCGGG‑GGGGCGGGTGGGTGCCGGCAACTGGCT‑GGTGGAGGCGGGCGTAACAAAAAAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCATAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATga < 2:737974/233‑1 (MQ=255) ggCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATgcaga > 2:307511/1‑239 (MQ=255) aaggtgggcgccggcgTGATGGCTGACAAACCGCT‑GGCGAAGCGGGGCGTAACCAAAGAAGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAaca < 2:608654/226‑1 (MQ=255) ggctggaAAGTCGCT‑GGCTGAGCAGGGCTTACCAAAAGAGGTTACCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCg < 2:300074/239‑1 (MQ=255) gcAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGCGGAAGTCATGGGCGTGGGCCGCACCTTCCCTGCAGCGTTTGCCAAAGCGCAACCGGGCCGAAACCCCACCCTGAAGAAACACGGGCGGGCACGGCTTACCGTgcgg > 1:491140/1‑238 (MQ=255) aagtggtgggcgggggggggcaaaaCAAAAGATGTTCCCCCGCCGTAGTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGa < 1:499504/216‑1 (MQ=255) aacggcgcgggggAGCAGGGCGACACGAAAGAATTTGACCCGCCGTACTACCGGGTGAAAGAAGGGGGTCCGCCGTTCAATAAATTCCCGGGCGGTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGGCATGGGCGGGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGa < 1:415044/227‑1 (MQ=255) aaagcgtgggcggggCAGAGCGAACCAAAATAAGCTACCCCGCCGTTCTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGcgcg < 2:329921/224‑1 (MQ=255) aTCGCT‑GGTGGAGCAGGGCGTAACAAAAGAGGTGCGCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAg < 2:692457/239‑1 (MQ=255) ctgtgctgAGCGGGGATTACCTAAGAAATTTACCCCTCCGTACTACTCGGGGAAAGAAGTGGTGCTGCCTGTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTTGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCg < 2:558312/235‑1 (MQ=255) tgggggggggCGTAACCAAGAAATTTCCCCCGCGGTTCTCTGCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGCCCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATaa < 1:452292/231‑1 (MQ=255) aggcgtggCGTAACGAAAGAAGTTCTCCCGCCGTACTGCTCGGTTAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAg < 2:590583/232‑1 (MQ=255) cGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCgtgg > 2:232493/1‑239 (MQ=255) aaCCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCCGCTTTCCGTGGCCGAAGGCGATAAAAAAcggggggggg > 1:881413/1‑231 (MQ=255) | CAGGAAATTCAGGATGTGATGCGCCAGCAGGTGCAGAAACTGGCCTTCGAATTGCAGGTGCGCGGCCTGATGAACGTGCAGTTTGCGGTGAAAAACAACGAAGTCTACCTGATTGAAGTTAACCCGC‑GTGCGGCGCGTACCGTTCCGTTCGTCTCCAAAGCCACCGGCGTACCGCTGGCAAAAGTGGC‑GGCGCGCGTGATGGCTGGCAAATCGCT‑GGCTGAGCAGGGCGTAACCAAAGAAGTTATCCCGCCGTACTACTCGGTGAAAGAAGTGGTGCTGCCGTTCAATAAATTCCCGGGCGTTGACCCGCTGTTAGGGCCAGAAATGCGC‑TCTACCGGGGAAGTCATGGGCGTGGGCCGCACCTTCGCTGAAGCGTTTGCCAAAGCGCAGCTGGGCAGCAACTCCACCATGAAGAAACACGGTCGTGCGCTGCTTTCCGTGCGCGAAGGCGATAAAGAACGCGTGGTGG > NC_000913/33223‑33691 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |