Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 361,668 | C→T | 18.6% | E65K (GAA→AAA) | lacA ← | thiogalactoside acetyltransferase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 361,668 | 0 | C | T | 18.6% | 145.2 / 6.6 | 71 | E65K (GAA→AAA) | lacA | thiogalactoside acetyltransferase |
Reads supporting (aligned to +/- strand): ref base C (10/46); new base T (10/4); total (21/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.38e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCG > NC_000913/361434‑361905 | gACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTAcc < 2:16920/239‑1 (MQ=255) cgaatcaggttgtttttgcAATCGTTAGCGGAAAAGGATTCCTCCCGGCGTTTTTTCTAAATTCATGGTGTACAGGGTGTCCAGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCg < 1:170636/221‑1 (MQ=255) cGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCg < 2:77097/239‑1 (MQ=255) tCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑tt < 2:156085/238‑1 (MQ=255) tATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGGTTTTCCCAAG‑GCG‑TTTTCCCCtc > 2:153072/1‑237 (MQ=255) aTCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTTGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTACATGGTTGTTGGCACCGCAAGAAAAATAGACAGGC‑GGGTATACACCA‑GCG‑TTTTTCCCC‑CCC‑CG‑GGCaaa > 1:33304/1‑239 (MQ=255) ctttttCGGAAAAGAGTACCTCTCGCCGTTTTTTCTCAATTCATGGTGTACCGGGTTGCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCCCCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑a < 1:104211/234‑1 (MQ=255) ctcGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATCTGGATGTTGGAACCGGAAGAGAAATAGACAGGC‑GGTTCTTCCCAG‑GCG‑GTTTACCCTCCCC‑GG‑GGCAAAC‑ATTTTTTT‑AAtaggcgttt > 1:131902/1‑229 (MQ=255) gCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTAccc < 1:714974/188‑1 (MQ=255) gCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTAccc > 2:714974/1‑188 (MQ=255) cGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGAGCAATAAGTAAGTTATCACCGATTGTTACCGTGTAGTAACCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATCTGGATGTTTGAACCGTAAGAGAAATAAACAGGG‑GGTCTTTCCCCA‑GCG‑TTTTTTCCC‑ACC‑CTGGGCAAAC‑Attttttatacatggtttagtttttt > 1:583912/1‑220 (MQ=255) tctcAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCAGCGGG‑TTTTCCCTT‑CCG‑GG‑GGGAAAA‑ATTT‑TTT‑ATT‑TAGGCATTTTTCtttttttcact > 2:20891/1‑234 (MQ=255) gtACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGAAAGGC‑GGTTTTACCCCA‑GCC‑TTTTTCCCT‑ACC‑CG‑CGCAAAC‑AATTTTTT‑TAA‑CTAGC‑ttttttttttttttaccctttcttggtgggaa > 1:147206/1‑219 (MQ=255) gggtggtCCGGTAA‑GGAAAAAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATTGACAATGGTTAAATTGAAATTTGCGTAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGT‑GGTTCGACACAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTATCAACTTCTGATGG‑ATGCGAGTGAt < 1:893868/233‑1 (MQ=255) gtCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCCG‑GCG‑GTTTTCCCT‑ACCAGT‑GCCAACA‑ATTTC‑TT‑AAT‑TAGGC‑TTTTTTTTTTTTTAACCTCTtatgg‑aaggcaaggtgtaaat > 1:590288/1‑224 (MQ=255) tCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTTACCAC‑GCGCTTTTCCCCT‑ACC‑TG‑GGCAACA‑ATTTTTTTCTAT‑C‑GCT‑TTTCTTTTTTTTCAACtttttttgaatcgcagagataaaac > 1:353850/1‑216 (MQ=255) cccGTAACGGAAATAGTATCGTTGGGGCCAATCATTTCCTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGTATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAT‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTc < 1:219832/239‑1 (MQ=255) cccGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTTCCCCA‑GCG‑TTTTTCCCT‑CCC‑CG‑GGCCAAC‑ATTTTTTT‑TAT‑ATGGA‑TTTTTCTTTTTTTAACTTTTgtttgcttcaggtgattcaaaca > 2:574882/1‑217 (MQ=255) cccGTAACGGAAAGAATAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTc < 2:33304/239‑1 (MQ=255) cccGTAACGGAAAAAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTc < 2:745885/239‑1 (MQ=255) ccttAACGGAAAGGGGAACTTTGGGTTCAATCAGTACGTTAACACCGTTTTTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCa < 1:749242/236‑1 (MQ=255) ccGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTTCGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCa < 2:88276/239‑1 (MQ=255) aCGGAAAAAGTAACGTTGGGTTCAATTATTTCGTTAACACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑At < 1:153072/239‑1 (MQ=255) tGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGa < 2:864563/239‑1 (MQ=255) ggTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCCG‑CCG‑TTTTTCCCT‑ACC‑CC‑GAGAAAC‑ACTTTTTT‑CAT‑ATCGA‑TTTCTCTTTTTTTACATTTCGATGG‑GTGCAAGTGTTT‑AAAATCATaaaaaaacaatttcacccaaca > 1:597693/1‑218 (MQ=255) aCGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCgg < 2:47379/239‑1 (MQ=255) cggtATCCCCGATTGTTACCGTGTAGTCATCGACAAGGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑‑TTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTa < 2:353906/236‑1 (MQ=255) gttacttgccttttttACCTTTTTGTCATCGACAATGGTTAAATTGAAATTTGCAAAATAATTGCGGCCTATATGGTTGTTTGAACCGGAAGAGAAATAGACAGGC‑GGTTCTTCCCAA‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCAAGAAGTCTTTTTTCCGGTa < 1:439869/225‑1 (MQ=255) caacGATTGTTACCGTGTAGTCAGCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑t < 2:578426/236‑1 (MQ=255) aCCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGt < 2:332606/232‑1 (MQ=255) ttttttcccGTTTTGTCTTCGACAATGTTTAAATTGAAATTTGCATAAAAATTGCGGCCTGTATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑TGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGca < 1:336172/232‑1 (MQ=255) aTTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTTCCCT‑ACC‑CG‑GGCAAAC‑AATTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGGAGGATT‑AAAATCATAA‑CTT‑AAC‑GTTTTCCCCCGAAGTCTTTTTTTCGTTAAGCC‑TTctcc > 2:784220/1‑236 (MQ=255) ttGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCAG‑CCG‑GTTTTCCCA‑CCC‑GT‑CGCAACA‑AATTTTTT‑AAT‑ATGGC‑GTTATCTTTTTTTAACTTCTTATGT‑GAGCGGTTGTTT‑ATACTCATAA‑ATT‑TAA‑ATTTTCTCACGCTATTTTTTTTTCGGTAATCC‑TTCGcca > 1:92975/1‑237 (MQ=255) ttGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGca < 2:147206/238‑1 (MQ=255) aCCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCCC‑GCG‑TTTTTCCCC‑CCC‑GG‑GGGAAAC‑AATTTTTT‑TTT‑CAGGC‑CTTCTTTTTTTTTAACTTTTTTTGG‑ATTCGAGTGGTT‑TAACTACTCC‑CTTAAAG‑GTTTTCCACCAAAGCATTTTTTCCGG‑AAGccattcccccagt > 1:332606/1‑227 (MQ=255) cGTGGAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCC‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCg < 2:56744/170‑1 (MQ=255) tgtAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCAA‑GCG‑GTTTCCCCT‑ACC‑CT‑GGAAAAC‑ATTTCTTT‑TAT‑TAGGC‑TTTCTTTTTTTTTCACCTCTTCTGGAATGC‑AGTGATT‑AAAATCATAC‑ATT‑AAA‑ATTTTTCCCCAAAAACTTTTTTTTGTTAAACC‑TTTGccaaaaaatgta > 1:247777/1‑228 (MQ=255) tgtAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCa < 2:766561/182‑1 (MQ=255) tCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACata < 2:114240/228‑1 (MQ=255) cATCGACAATGGTTAAATTTAAATTTGCATAAAAATTGCGGCCTATTTGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAg < 2:748504/238‑1 (MQ=255) gACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTTCCCT‑ACC‑CT‑GGCAAAC‑ATTTCTTT‑TAT‑CAGGC‑TTTTTCTTTTTTCAACTTCTGATGG‑ATGCGGGTGATT‑TAACTCCTAC‑ATT‑TAC‑GTTTTTCCCCCAAGGCTTTTTTTCGGGAAGCC‑TTCCCACATATAGGTAAAAAGCTTg > 2:324576/1‑238 (MQ=255) aCAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGcc < 2:687329/239‑1 (MQ=255) cAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGcc < 2:278854/238‑1 (MQ=255) tGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCCG‑GCG‑GTTTTCCCC‑ACC‑GT‑GGCAAACAATTTTTTT‑AAT‑‑AGGC‑TTTTTCTTTTTTTAACTTCTGTTGG‑ATGGGAGGGATTAAAACTC‑TAC‑ATT‑AACTGTTTTCCC‑CCAAAACTTTTTTTCGTTAAACCATTCCcacaaacgggaaaaaaagatctctta > 1:114240/1‑217 (MQ=255) tGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTTACCCG‑GCG‑GTTTTCCCC‑ACC‑GT‑GGGAAAA‑ATTTTTTT‑TATAAAGGC‑TTTTCTTTTTTTTAATTTCTTTTGG‑‑TGGTGGGGGTT‑AAAATCCAACAATT‑AAC‑TTTTTCCCCCCAAG‑CTTTTTTTCTGGAAACC‑CTCCCCCAAATAGGTAAAAAACATGCCTg > 1:720316/1‑237 (MQ=255) tGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCtt < 2:247777/239‑1 (MQ=255) ttgaaaaaaaTTGGGGTCTATTGGGTTTTTGGAACCGTTAAGGAAAGAGACCGGC‑TGTTTTTCCCAG‑GCC‑TTTTCCCCT‑ACC‑GG‑GGAAAAC‑ATTTCTTT‑AAG‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 1:125497/234‑1 (MQ=255) ttcaaaaaaaTTGCGGCCTTTATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 2:583912/234‑1 (MQ=255) ttcATAAAAATTGCGGCTTTTAGGGATTTTTGAACCGTAAGAGAAATAGACCGGC‑GGTTCTCCCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 1:784220/237‑1 (MQ=255) tGCATAAAAATTTCGGCCTATATGGATGTTGGAACCGTAAGAAAAATAGACAGGC‑GGTTCTTCCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 1:526733/239‑1 (MQ=255) tGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 2:250106/239‑1 (MQ=255) tGCAAATAAATTGTGGCCTTTATGGATGTTGGAACCGTAATAAAAATGGACAGGC‑GGTTCGACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCa < 1:58808/239‑1 (MQ=255) tAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTgg < 2:723700/239‑1 (MQ=255) ggCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCaa < 2:720316/238‑1 (MQ=255) ccTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGTAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTa < 1:59803/192‑1 (MQ=255) tttttgATTTTGGAACCGTAAGAGAAATAGTCAGGC‑TGTTCTACCCAT‑GCG‑TTTTCCCCT‑ACC‑TG‑TGCAAAC‑ATTTCTTT‑AAT‑CAGGT‑TTTCGCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGAt < 1:308442/234‑1 (MQ=255) gggtGTTTGAACCCGTAGAGAAATAACCCGGG‑GGTTCTACCCAT‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCGTT‑AAT‑CAGGC‑TTTCTCGTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt < 1:6285/236‑1 (MQ=255) ggATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTTCCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt < 2:353850/239‑1 (MQ=255) ggATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt < 2:92975/239‑1 (MQ=255) ggATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACt < 2:590288/239‑1 (MQ=255) ttttGGAACCGTAAGAGAAATAGACAGGC‑GTTTCTTCCCAG‑GTG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGGGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg < 1:34791/237‑1 (MQ=255) ttttGAAACCGTAAGGAAAAATGAAAGGC‑GGTTTTCCCCAG‑GCG‑TTTTCCCCG‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAG‑CAGGC‑TTTCTCTTTTTTCAACTACTGATGG‑ATGCGAGTGATT‑AAACTCATGC‑ATT‑AAC‑GTTTTCCCAAGAAGTCGTTTTTCCGGTAAGCC‑TTCGCACATAGCGGTAAATGGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTcc < 1:20891/236‑1 (MQ=255) tGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCTT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg < 2:572170/239‑1 (MQ=255) tGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTcc < 2:167977/238‑1 (MQ=255) gTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTTTACCCAG‑GCT‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTTTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCg < 2:346170/238‑1 (MQ=255) ttGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTTCCCC‑ACC‑CT‑GGCAAAA‑ATTTCTTT‑AAT‑CAAGG‑TTTCTCTTTTTTTAACTTTTTATGG‑ATGCGAGTGGTT‑AAACTCATAC‑ATT‑AAA‑GTTTTTCCCCGAAAGCTTTTTTTCGGTAAACC‑TTTGCCCATATCGGTAAAAAGCTTGCCTGCTCTTATTTTTTTGGTCATTGGCATGGTCAATGCTATCACTCCCtt > 2:119578/1‑239 (MQ=255) ggAACCGTAAGAGAAATAGACAGGC‑GGTTCTAACCAG‑GCG‑GTTTTCCCC‑ACC‑CG‑GGGAAAA‑ATTTTTTT‑TAT‑TATGG‑TTTCTTTTTTTTTCACTTCTGATGG‑GAGCGGGGGATT‑AAACTCATTA‑ATT‑AAC‑GGTTTTCCCCCAAGTCTTTTTTTCCGGAACAC‑TTCGCCCCCACTCTTAAAAAACTTGCCTTCTCTTATTCTTTTTTTCTTTGCGATGTTAAATgggctaaccccgcttt > 1:690866/1‑224 (MQ=255) tAAGAGAAATAGACAGGC‑GGTTCTACCCCG‑GCG‑TTTTTCCCC‑ACC‑CT‑GGGAAAC‑ATTTTTTT‑TAT‑CAGGC‑TTTCTCTTTTTTTAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTTCCACGAAGTCTTTTTTTCCGTAAGCC‑TTCGCACCAATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGGTCAATGCGGTTACTCCCTTTTggttatt > 2:241763/1‑233 (MQ=255) aaaTAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTc < 2:431272/207‑1 (MQ=255) aaaTAAACAGGG‑TGTTTTCCCCAG‑GGG‑TTTTCCCCT‑ACC‑GT‑GGGAAAC‑ATTTCTTT‑AAA‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGt < 1:574882/237‑1 (MQ=255) tCTAGCCAG‑GCG‑TTTTCCCCT‑TCC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGT‑TTTCTCTTTTTTCAACTTCTGAGGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGGC‑TTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATGCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTcgcg < 2:805525/238‑1 (MQ=255) | GACTTCCGATCCAGACGTTATTGCCAATCGTTATCGGAAAAGAGTACATCTCGCCGTTTTTTCTCAATTCATGGTGTACAGGGTGTCCCGTAACGGAAAGAGTAACGTTGGGTGCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGC‑GGTTCTACCCAG‑GCG‑TTTTCCCCT‑ACC‑GT‑GGCAAAC‑ATTTCTTT‑AAT‑CAGGC‑TTTCTCTTTTTTCAACTTCTGATGG‑ATGCGAGTGATT‑AAACTCATAC‑ATT‑AAC‑GTTTTCCCACGAAGTCTTTTTTCCGGTAAGCC‑TTCGCACATATCGGTAAATAGCTTGCCTGCTCTTATTCTTTCGGTCATTGGCATGTTCAATGCGATCACTCCGTTATGATATGTTGGTCGGATAAGGCGCTCGCG > NC_000913/361434‑361905 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |