Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 728,493 C→A 17.7% M80I (ATG→ATT kdpA ← potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913728,4930CA17.7% 113.6 / 2.1 57M80I (ATG→ATTkdpApotassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (27/19);  new base A (2/9);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 2.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGC  >  NC_000913/728262‑728726
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
gCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCCTTCAGATTGCCCGTTGGTATTTGTGACAAAGCTGACGGCGGGATTTCGCCCCCGATCCCCCAGAAGCCCGTGCAAATGCCTGGGGTTAAGCGGGCGAAAGGGCCTGCCGAGCCACCTaaaaa                                                                                                                                                                                                                                      >  1:636039/1‑239 (MQ=255)
           aaCGGAGGAGGGCAAAAACCACCGCAACCCCGCTGGCGGAAGAAAGAAAGTTTGGCACCGTAAAGCCCGCCATCTGGCTGAAATAGCTAAACGTGGTTTCACCGCTAAAAGATGGCCAGTTGGTATTGGTGAAAAAGCTGACGGCGGTATCCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTCCTGACCGAGCAACAAAAAAAACAGCACCGcc                                                                                                                                                                                                                           <  2:730544/238‑1 (MQ=255)
            aCGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAACCTGCCGGCGGTATTAAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGAAATTGCTGACCGAGCAAAACAAAAAAAAGAACcccc                                                                                                                                                                                                                           >  1:261692/1‑238 (MQ=255)
                              gaacatgctggggCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAACGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCCTGTTCCgg                                                                                                                                                                                                         >  2:616770/13‑238 (MQ=255)
                                      tCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGGATTCAGCGCCCGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAATGCTGACCGAGCAAAAGAAAAAAAAGCACCGCCGGACCCAGAATGTTCAAACCGAGaaag                                                                                                                                                                                                >  1:415487/1‑237 (MQ=255)
                                                                tGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGGGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAAATTGCTTCCAGTTCa                                                                                                                                                                       >  1:168648/1‑238 (MQ=255)
                                                                   ccggTTAAGCCCGCCATTGGGCTGAAATAGCTCAACGTGTTTTCCCCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCGGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAtct                                                                                                                                                                    <  2:86086/235‑1 (MQ=255)
                                                                    ccGTTAAGCCCGCCTTCTGGGTGAAATGGCTAAACGTGGTTTCCCCGATAGAAAATTGCCAGTTGGTTTTGGGCACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGAAAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCa                                                                                                                                                                  <  2:415487/239‑1 (MQ=255)
                                                                       ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCCCGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGGGCCACATAAAAAACAGCACCCCCAGCCCCAGCAAGTTCAGGCCGAGAATGGCAAAAAAATATTACTTCCAGTACATCTCACgg                                                                                                                                                               >  1:386465/1‑239 (MQ=255)
                                                                       ttAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCCCAAAGATATTGCTTCCAGTTCATCTCACg                                                                                                                                                                >  2:97002/1‑238 (MQ=255)
                                                                        aaagataaaggtttgtgttaaaTTGCTCGACGGGTTTTCCCCGCTATAAGTGTGCCGGTTGGTATTGGGGACAAAGCTGACGGCGGTATTCAGCGCCTGTTCCCACGACCGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCGCACCGACCAACATAAAAAACAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGt                                                                                                                                                              <  1:59298/220‑1 (MQ=255)
                                                                          accccGCCTTTGGGCTAAAATAGCGCAACGGGGTTTACCCGCTAAAAGATTGCCAGTTGGTATGGGTGACAAAGCTGACGGCGGTATTCAGCCCCAGACCCCACGACAGCCCTGGCAACTGCTGGGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCa                                                                                                                                                            <  2:259492/237‑1 (MQ=255)
                                                                               gCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGGGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGCGAATGGCACAAAAATATTGCATCCAGTTCATCTCACGGTCAGAGAc                                                                                                                                                      >  2:761297/1‑239 (MQ=255)
                                                                                          taattaccacaatggtttttCCCCGTTAAAAGGTTCCCGGTTGGTTTTGGGAAAAAAGGTGGCGGGGGTTTTCAGCCCCGACCCCCCCGACAGCCCTGGAACCTGCTGTGGATTAAGCGGCAGATAGGGCTGACCGAGCAAAATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTgcgc                                                                                                                                           <  1:84612/222‑1 (MQ=255)
                                                                                             aTAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCCCAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCactgtatctta                                                                                                                                        >  2:59298/1‑229 (MQ=255)
                                                                                                 cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCCCGGTCAGAGACCCCAAAAGCGCGaaaaaa                                                                                                                                    >  1:839976/1‑238 (MQ=255)
                                                                                                 cTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAATGCTGACCGAGCAACATAAAAAACAGCACCCCCAACCCCAACCTGTTCAGGCCGAGACGGGCCAAAAGAAATTTGTTCCCATTCATCTCCCCGCCAGGAGAGCCAAATGGGCGgaaaaa                                                                                                                                    >  1:691327/1‑238 (MQ=255)
                                                                                                      cGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCCGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAAATTGCTTCCCGTTCATCCCCCGGGCAGAAACGCCAAGGGCGCGaaaaaaaaagg                                                                                                                               >  1:463615/1‑233 (MQ=255)
                                                                                                             tccccGCTAAAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAAc                                                                                                                       <  2:580180/236‑1 (MQ=255)
                                                                                                                  gCTATAAGATTGCCATTGGGTATTGGTGACAAAGCTACCGGCGGTATCCAGCGCCAGACCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACAAAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCg                                                                                                                  <  2:385010/239‑1 (MQ=255)
                                                                                                                       aaGATTGCCGGTGGGTGTGGGTGACAAAGCTGACGGCGGTATTGAGCGCCAGACCCCACGAAAGCCCTGGCAACTGCTGTGAATTACGGGGCAGATAGTGCTGACGGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTa                                                                                                             <  2:706903/239‑1 (MQ=255)
                                                                                                                       aaGATTGCCAGTTGGTTTGGGGGACAAAGCTGACGGGGGTATCCAGCGCAAGATCCCACGAAAGCCCTGGAAACTGCTGTGGATTAAGCGGCAGATAGGGCTGCCCGAGCAAAATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTa                                                                                                             <  2:636039/239‑1 (MQ=255)
                                                                                                                             gCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAAATTGCTTCCATTTCATTTCCCGGTCAGAACCGCCAAGGGGCCGAAAAAAAAACCGCCAAAACCCCGTTGTAACgggg                                                                                                       >  1:304035/1‑238 (MQ=255)
                                                                                                                              ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGCGCAACATAAAAAACAGCACCGCCAGCCCCAGCCTGGTCAGGCCGAGAATGGCACAAAGATATTGCTTCCCGTTCATCTCCCGGTCAGAGAAGGCACGTTCGGGACAAAAAACGCGCCCAAAGCCCCGTGGACCggggg                                                                                                      >  1:832686/1‑237 (MQ=255)
                                                                                                                              ccAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGaa                                                                                                      >  2:175672/1‑239 (MQ=255)
                                                                                                                                  ttGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGAGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGGGAATGGCACAAAGATATTGCTTCCCGTTCATCTCACGGTAAGAGACGCCAAGTGCGCGAAAAAGAACGCGCTCAAAGCCCGTTTTCCCGGCACGAgg                                                                                                  >  2:884501/1‑239 (MQ=255)
                                                                                                                                   tGGTTTGGGTGACAAAGCTGCGGGGGTTTTCACGCACAAGACCCCCCGACAGCCCGGGAAACTGCTGGGCATAAAGGGGAAGATTGTGCTGACCGAGCACAAAAAAAAACAGCTCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGa                                                                                                 <  1:712333/239‑1 (MQ=255)
                                                                                                                                   tGGTAGTGGATACAATGCTGACGTGGTTATCCAGCCCCAGACTCACCAACAGCCTTGCTAAACGCAGGACATTAGACGGCATGTGGTGCTGACCGAGCAACATAAAAACCAGCCCCGCCAGCCCAAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGa                                                                                                 <  1:775866/239‑1 (MQ=255)
                                                                                                                                    gtttttGGTGACAAAGCTGACGGCGGTATTCGGCGCCAGATCCCACGACAGCCCTGGCAACGGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa                                                                                                <  2:373185/235‑1 (MQ=255)
                                                                                                                                    gggtttGGTGACAAGGGTGGCGGCGGTATTCAGCGCCAGACCCCACGAAAGCCCTGGAACCTGCGGTGGATTAAGGGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa                                                                                                <  2:386465/235‑1 (MQ=255)
                                                                                                                                    ggctttgtggaaaaaGGTGAGGGGGGTACTCAGGCCCAAATCCCACGACAGCCCTGGCAACTTCTGGGGATTAGGCGGCAGATAGGGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGaa                                                                                                <  2:691327/227‑1 (MQ=255)
                                                                                                                                          ggTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACAACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGACCGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCAt                                                                                          >  2:71466/1‑239 (MQ=255)
                                                                                                                                                aaaGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCCCGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATc                                                                                     >  2:466549/1‑238 (MQ=255)
                                                                                                                                                 aagggggggggggTATCCAGGGCCACACCCCAAGCAAGCCTGGGAAATGCTTGGGGATAAGCGGGAGATTAGTGCTGCCGGACCAAAAAAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAg                                                                                   <  1:491889/228‑1 (MQ=255)
                                                                                                                                                        cggcggTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAACCAGCACCGCCAGCCCCAGCATGTTCAGGACGAGAATGGAAACAAAAAATTGCGTCCAGTTCATCTCACGGTCCGAGACGCCAAACGCGGGACACAGTACGGCTCCAAACCCCGTTGGACAGGGACGAGTAAAGTAATAAAACAGccacacc                                                                            >  1:732470/1‑234 (MQ=255)
                                                                                                                                                         ggcggTATTCAACGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAAAAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGAAATTGCTTCCAGTTCATCTCACCGTCAGAGACGCAAAGTGCGCGAAAAAGTACGCTCTCAACGCCCGGTGTACCGGGAAGAGGGAGGTCATAAAACAGCAGCGCCa                                                                           >  1:718150/1‑239 (MQ=255)
                                                                                                                                                               tttCCGCGCCGGACCCCAGGACGGCCCTGGAACCGGCGGTGGATACAGGGGCAGGTAGGTCTGCCCGAGCAAAATAAAACACAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCg                                                                      <  1:616770/237‑1 (MQ=255)
                                                                                                                                                               tttCCGCCCCAGTCCCCAGGAGAGCCCGGACAACTGGGGTGGGTTAAGGGGCAAAAAGGCGTGACCGAGCAACAAAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCAGCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGc                                                                     <  1:537841/238‑1 (MQ=255)
                                                                                                                                                               aTTCAGCGCCAGATCCCCCGCCAGCCTGGGCAACTGCTGGGGATTAGGCGGCAGATGGGGCTGACCGATCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGc                                                                     <  2:774721/239‑1 (MQ=255)
                                                                                                                                                               aTTCAGCGCAAGATCCCACGACAGCCCTGGCAACTGTTGTGGATTAAGCGGAAGATAGTGCTGACCGAGCAAAAAAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGc                                                                     <  2:304035/239‑1 (MQ=255)
                                                                                                                                                                             ccccaggaaggcccggcaaaCTGCGGTGGATTAAGCGGAAGATAGTGCTGACCGAGAAAAAAAAAAACCAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGa                                                          <  2:261692/219‑1 (MQ=255)
                                                                                                                                                                                    aagccccTGGCAACCGGTGTGGATTAAGGCGCAGATGGTGCTGACCGGAAAAAAAAAAAAACAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGc                                                 <  2:727346/235‑1 (MQ=255)
                                                                                                                                                                                     cAGCCCTGGCAACGGTGGTGATTTAACGGGCAGATAGTGCTGCCCGACCAACATAAAAAAAAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCa                                                <  2:896661/239‑1 (MQ=255)
                                                                                                                                                                                     acgCCCGGGAAAGTGGTGGGGGTTAAGCCGCAGAAGGGCGGGACGGAGCAAACACAAAAAAGGGCCCGGCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCa                                                <  2:271089/237‑1 (MQ=255)
                                                                                                                                                                                        cccTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAACGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTTCCTACAGGACGCGCCAGCCCCa                                             >  2:524301/1‑239 (MQ=255)
                                                                                                                                                                                         ccgggCAATTGATGGGGATTAAGCGGCAGATGGTTCCGACCGAGCAACAAAAAAAACCGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGc                                                 <  2:683640/231‑1 (MQ=255)
                                                                                                                                                                                                  ttctgTGTATTAAGGGACAGTTGGTGTTGCCCGAAAACAATAAAAAACAGACCCGCCAGCCCCCGCATGCTCCGGTCGAGAATGGCACAAAGATATTGCTTCCAGCTCATCGCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCCACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAg                                   <  1:113844/237‑1 (MQ=255)
                                                                                                                                                                                                              aGCGGCAGATAGTGCTGACCGAGCCACATAAAAAACCGCACCGCCAGCCCCAGCCTGTTCAGGCCGAGAATGGCACAACGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCCCGAAAAAGTACTCGCTCCACGCCCGTTGTACCGGTCAGAGGAATGTCATTTATCAGCCGCCCCCGCCCGCGTCCTACAAGACCCGCCCGACCCAATAACACCACTTAAAACATgtc                       >  1:796194/1‑237 (MQ=255)
                                                                                                                                                                                                                   cAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGCTCa                  >  2:91171/1‑239 (MQ=255)
                                                                                                                                                                                                                    gcagagaggtcggACCAAGCAACATAAAAAACAGCCCCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGAt                    <  2:832686/226‑1 (MQ=255)
                                                                                                                                                                                                                       tgtagccctctccGAGCACCACAAAAACCAGCCCCGCCAGCCCCAGCTTGTTCAGGCCGGGAAGGGCACAAAGATATTGCTTCCAGTCCATCTCACTGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGt                <  1:761297/228‑1 (MQ=255)
                                                                                                                                                                                                                         aGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGAAGCGCCAGCCCCATTAAAACACGAAAAAACGGGGGGaccggaaaa             >  1:498892/1‑231 (MQ=255)
                                                                                                                                                                                                                               tGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCCTTAACACCAGTAAAAACAAGGCGACCaaaaaaaac          >  1:749921/1‑229 (MQ=255)
                                                                                                                                                                                                                                 aCCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTTAAGGACGCGCCAGCCCCATTAACACCAGGAAAAACGGGGCGACCAGAAAGAACCCtt      >  1:562664/1‑239 (MQ=255)
                                                                                                                                                                                                                                      gCAACATAAAAAACAGCACCGCCAGCCCCAGTATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTtcgc  >  2:678070/1‑238 (MQ=255)
                                                                                                                                                                                                                                      gCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTgcgc  >  2:310415/1‑238 (MQ=255)
                                                                                                                                                                                                                                      gCAACATAAAAAAAAGCACCGCCAACCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATAATGCTTCCAGTTCATCTCACGGTCAGAGACCCCAAGTGCGCGAAAAAGTACGCGGTAAACGCCACCTGTACCGGACAGAGGACTGTCATTAATCAGCCGCGGACACCCACTGCCCAAGGGTCGCGCCCGCCACATTACCACCATTAAAAAAAGCGAGAACAGAAAGAACCCtgtcgc  >  1:133228/1‑233 (MQ=255)
                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                           
GCGGGTAAACGCACGGATGAGGGCAAAAATCACCGCAATCCCGCTGGCGGCAGAAAGAAAGTTTTGCACCGTTAAGCCCGCCATCTGGCTGAAATAGCTCAACGTGGTTTCACCGCTATAAGATTGCCAGTTGGTATTGGTGACAAAGCTGACGGCGGTATTCAGCGCCAGATCCCACGACAGCCCTGGCAACTGCTGTGGATTAAGCGGCAGATAGTGCTGACCGAGCAACATAAAAAACAGCACCGCCAGCCCCAGCATGTTCAGGCCGAGAATGGCACAAAGATATTGCTTCCAGTTCATCTCACGGTCAGAGACGCCAAGTGCGCGAAAAAGTACGCGCTCAACGCCCGTTGTACCGGGAAGAGGAATGTCATTAATCAGCCGCGCCAGCCCGCTGCCTAAAGGACGCGCCAGCACCATTAACACCAGTAAAAACGTGGCGATCAGTAAGAACCCTTGCGC  >  NC_000913/728262‑728726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: