Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 742,267 | T→C | 14.4% | E97G (GAG→GGG) | dtpD ← | dipeptide and tripeptide permease D |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 742,267 | 0 | T | C | 14.4% | 163.7 / 3.7 | 73 | E97G (GAG→GGG) | dtpD | dipeptide and tripeptide permease D |
Reads supporting (aligned to +/- strand): ref base T (41/20); new base C (2/10); total (43/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.44e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC > NC_000913/742037‑742497 | ccAGGCCAACGCCCAGCGCCCAGCTGTACTCTCCCTGGGCGAACCCACAGGCGATAGGTGCAATAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCa < 2:660268/239‑1 (MQ=255) cAAAGCCCAGCGCCCAGCTGTCCTCTCCCGGGGCGTAACCACAGCAGAAAGGTGCAATAATAGCCCCCCCGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg < 2:576308/238‑1 (MQ=255) cAAAGCCCACCGCCCCGCTGTACTCTCCCTGGGCGTAACCACAGGGAAAGGGGGCGAAAATAGCCCCCACGTTCCCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg < 2:813816/238‑1 (MQ=255) aaaaGCCCACCGCCCGGCTGTACTCCTCCTGGGCATAACCGAAGGGGGTGGGGGCAATAATACCCCCCACTTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAg < 2:163537/237‑1 (MQ=255) aGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCCCCCGGGGCAGATAAAGGAATGACGGATGGATCTCAATGGCACCCAGcaccaacacaccgaa > 1:794040/1‑228 (MQ=255) tACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCAt > 2:775241/1‑239 (MQ=255) ccccctcccggggcaaaacccagggCGATAGGTCGAATAATAGACCCACCTTTACCCGCCGCAAACATCCGCGAAAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCAt < 2:723012/216‑1 (MQ=255) ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATcacc‑aa > 2:761587/1‑238 (MQ=255) ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGACACCCCGAACCACA‑AGACCGATGGACATCaaa‑aac > 1:363065/1‑239 (MQ=255) ccTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAAAGGAATGACGGCTGGATCCCACTGGACCCCCACACCACA‑TAACCGACGGCCACcaaa‑aaa > 1:276949/1‑238 (MQ=255) cGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATATAGGAATAACGGATGGATCTCACTGGCACCCATCACCAAA‑TGACCGAACGCCATCAAA‑AACGccccc > 2:370558/1‑239 (MQ=255) tAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGCCAGCTTACGGTAGATTTAAACAGGCCATAGCCGCAGACGACAACCGCCAGGGACAGATACAGGAATGCCGGATGGATTCCCATGGCACCCAGCACCACA‑TGACGGATCGCCATCAAA‑AACCCCCCCac > 1:194195/1‑238 (MQ=255) aCCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGGTCTCACTAGCACCCAACCCCACA‑TAACCGCACACAATCAAC‑AACGcaccccaca > 1:81162/1‑235 (MQ=255) aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCATGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑AGACCGATCGCCATCAAA‑CACGCCCCCCGCCTCACCCCCacgg > 1:629107/1‑236 (MQ=255) aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATGGCCAGGGACAGATAGCGGAATGACGGATGGATCTCACTGGGACCCA‑CGCCACACTGACCGATCGCCACAAAA‑AAACCCCCAAACAACACCGcacagg > 1:14389/1‑234 (MQ=255) aGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAA‑AACGCCCCCAGCATCACCGCCATGc > 2:705835/1‑239 (MQ=255) cGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAACGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACA‑TAACGCATCGCCA‑CAACAAACGCCCACAGCACCACCCCCAAGcagagc > 1:436068/1‑234 (MQ=255) aGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGCATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑ACGGCCCCCAGCATCACCGCCATGCGATTGACgcga > 2:286747/1‑238 (MQ=255) gACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCCGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTAACTGGCACCCAGCCCCACA‑AGACCGATCGCCATCAAC‑AACCCCCCCAGCACCACCCCCaaccacataccgcaa > 1:83030/1‑223 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGGTGGATCTCACTTGCCCCCAGCACCCCA‑GGCCCGATCGCCATCACA‑AAACCCCCCCGCATCACCGCCATGCGAAGGCCGAGAACATTa > 1:454902/1‑238 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCCACCCCATGCGAATCCCGGAGACTTTac > 1:284214/1‑238 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCACCACCGCCATGCGATTGCCGAGACCTTTa > 2:205548/1‑238 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCCCCCTCAAC‑AACGCCCCCACCATCACCCCCAGGCGGTTGCCGGAAACttaa > 1:487199/1‑236 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACAACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGAATCACCGCCATGCGATTGCCGAGAACTTTa > 2:204520/1‑238 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGGTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGCCAGATAAAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGGTCGCCATCAAC‑AACCCCCCCCGCATCCCCGcacaacaccttccggaaacttta > 1:543884/1‑216 (MQ=255) cACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCCCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACCGCTAGAGGAATGCCGGATGGATCTCACTTGCACCCAGCACCACA‑TGACCGATCGCCAGCAAC‑AACCCCCCCAGCATCACCGCCACGCGCTTCCCGAGAACtttc > 1:670098/1‑237 (MQ=255) cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCCGCATCACCGCCATGCGATTGCCGAGAACTTTATc > 2:304475/1‑238 (MQ=255) acccgcccgaataatccgggagaACCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTGGCCGAGCAGGCAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCa < 2:263041/220‑1 (MQ=255) gccgcATACATCAGCGAGAAACCGCCATCACGAAGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTAACCGCCaaaaa > 2:725864/1‑239 (MQ=255) cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGCCGATAATCGCCAGGGACAGATAGAGGAAGGACGGATGGATCTCCATGGCACCCATCACCACA‑TGACCGAACGCCCTCAAC‑AACGCCCCCAGCCTCCCCGCCATACGACTGACGAGAACTTTACCCCCCaaaaaa > 1:853565/1‑238 (MQ=255) cgcATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCCCA‑TGACCGAACGCCCTCAAC‑AACGCCCCCAGCATCACCCCACTCCCATTACCCAGCACTTTATCCACCAAAAAg > 1:110148/1‑238 (MQ=255) tACATCGGCGAAAAACCGCCATCACGGCGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCAcc < 2:487199/239‑1 (MQ=255) agggagaACCCGCCATCACGACGCGGATCGGTT‑GGCTAATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGAt < 2:194195/236‑1 (MQ=255) cGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATCCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTg > 2:16805/1‑239 (MQ=255) gaaaaaCCGCCATCCCGACGGGGATCGGTT‑GGCTAATCAAGCTCGCCGAGCAGACAGTTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGa < 2:794040/232‑1 (MQ=255) agaAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGACCTTTATCGGCCAAAAAACCACAGAGGATTGGc > 2:829573/1‑239 (MQ=255) aaaaCCGCCAACACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCg < 2:276949/238‑1 (MQ=255) aaaCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCCAC‑AACGCCCCCAGCATCCCCGCCATGCGATTGCCGGGCACTTTATCCGCCAAAAAGCAACCGAAGAAGGGCgg > 1:54242/1‑238 (MQ=255) aaCCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATCCGATTTCCGGGAACTTTATCCGCCAAAAAAACACCCAGGATTGGccgg > 1:98849/1‑236 (MQ=255) tCACGACGGGGATCGGTT‑GGCTCATACAGCTCCCCGAGCAGACAGCTTACGTTAGATTTAACCAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATacac < 2:584125/239‑1 (MQ=255) acgCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGGGAAATTTATCCGCCAAAAAGCCACCGGGGATTGGGGTGAAATAAACCAGcc > 1:798646/1‑238 (MQ=255) cggggATCGGTT‑GGCTCATCAAGCTCGCCGGGCAGACAGCTTCCGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCg < 2:284214/235‑1 (MQ=255) tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCCATTGCCGGGAACTTTATCCGCCAAAAAGCCACCCAGGATTGGCGGGACATACACCCGCCaaaaaaaggggcaaaa > 1:10221/1‑225 (MQ=255) cccccccGAGCAAAAAGTTGATGTAAGATTAAAACAGCCCAGTGCCCGAAAGAAGAACCCCCAGGGACAGAAAGAGGAAGGACGGATTGACCTCACTGGCACCAAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg < 1:705835/234‑1 (MQ=255) tCGCCGGCCAGACAGCTTAGGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAGCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCg < 2:555177/237‑1 (MQ=255) cagcaagtctagttaaaaTTAAAACAGGCACACGCCGGAGACGATAACCCCAAGGGACAGAAAGAGGAATGCGGGATGGCCCCCACGGCCCCCCCGCACCACA‑AGCCCGGTGGCCATCAAA‑AACGCCCCCAGCATCTCCGCCATGCGATGGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg < 1:73274/222‑1 (MQ=255) aGACATCTTACGTTAGATTTAAACAGGCCATGGCCGGAGACGATAACCGCCAGGGAAAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAg < 2:588767/238‑1 (MQ=255) aacatttttcGTTAGTTTTAAACGGGCCAAGGCCCCACACGATAAACGCCAGGGAACGATAGAGAAATGACGGATGGATCCACCTGGCACCCAGCACCACA‑TGACCGACCGCCATAAAC‑ACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa < 1:16805/230‑1 (MQ=255) agtcTTGCGTTGGATTTAAACAGGCCATGCCGGCAGACTATAATCGACAGGGGCAGATGGGGGAAGGACGGATGGATCTCCCTGGCACCCAGCACCACA‑TGCCCGATCGCCATCAAC‑ACCGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTAt < 1:370558/235‑1 (MQ=255) aacgttACTTTAAATTTAAACAGGCCATGGCCGCAAACAATAATCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTa < 2:794599/233‑1 (MQ=255) agaagcaaaaatttaaaaCAGGCCAAGCCCGCAACCCAAAACCCCCAGGGAACAATAAGGGAATGCAGGAAGGACCCCCCTGGCCCCACGCCCCAAA‑GGCCCGATCGCAATAAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGt < 1:881711/224‑1 (MQ=255) ttACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACGGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 2:480949/236‑1 (MQ=255) atACGTTAGGTTTAAACAGCCAGTAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGCCGGATGGATCTCACTGGCCCCCAGCACCAAA‑TGACCGATCGCCTTCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCg < 2:697760/235‑1 (MQ=255) taaaTTTAAACGGCCCATAGCCACAGGCGATAATCCCCAGGAACAGAAAGAGGAAGGAAGGGTGGATCTCACTGGCACCCACCCCCACA‑GGACCGATGGCAACAAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTAttt < 1:254710/235‑1 (MQ=255) tgttttAAACAGGCCCTTGCCCCAAACGTTAACCGCCGGGGAAAGAGAGAGGAATGCCGGATGCATCCCACTGGCCCCCGGCACCCAA‑TGACCGATCGCCATCAAC‑ACCGCCCCCAGCATCCCCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTa < 1:761587/236‑1 (MQ=255) aacagggCCATAGCCCAAGACGAAAACCCCCAGGGCCAGAAAGGGGAATGACGGATGGATCCCCCTGGAACCCACACCCAAA‑TGCCCGACCCCCATCAAC‑AACCCCCCCAGCATCCCCGCCATGCGATGGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGAt < 1:204520/234‑1 (MQ=255) acgggCCATAGCCGAAGGCGATAAGCGCCAGGGACAGATAGGGGAATGACGGATGGATCTCACTGGCACCCAGCCCCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTg < 2:310674/236‑1 (MQ=255) aGGCCATAGCCGCAGACGATAACCGCCAGGGACAGATAGAGGAATGACGGATGGATCCCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGGGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGt < 2:10221/239‑1 (MQ=255) ggCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAg > 2:881711/1‑229 (MQ=255) aGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACCCCAGCGAACAGTAGGCGCAAAATAACACGTAGGCGTGGGTATCGGTGTATTTTAGTTGATTGgagag‑aaaa > 1:310674/1‑236 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTAATTGGGGAG‑AAAAAGaaa > 1:432441/1‑238 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCCCCGAGGATTGCGGGGACATACACCAGCGAAACGTAGCCGCTAAATAACTAGTAGGCGGGAGAATCGTTTTATTTTATTGGGTTGGGAAA‑AAAGAGaaa > 1:854028/1‑238 (MQ=255) cAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGCAGGCGTGAGAATCGTTGTATTTTAGTTGATTGGTGGG‑ATAGAGAAt > 2:123638/1‑239 (MQ=255) agaaaaTCCCCAGGGCAAGATAGAGGAATGCCGGATGGATCCCACTGGCCCCCAGCACCAAA‑TGACCGATCGCCATCAAC‑AACGCCCCAAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATcag < 2:432441/234‑1 (MQ=255) aagcGCCAGGGACAGATAGGGGAATGGCGGATGGATCCCAGTGGCACCCAGCCCCCCA‑TGACCGATCGCCTACAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAggg < 2:110148/236‑1 (MQ=255) aGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAAGCGCCAAATAACACGAAGGGGGGGGTACCGTTGTATTTTAGTTTATTGGTGAG‑CAAGAAAATACAAAGGGCACgaa > 1:311728/1‑236 (MQ=255) ggACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGGGAG‑ATAGAGAATCAGCAGGGCACGCATg > 2:79884/1‑238 (MQ=255) aattgggggAATGAAGGTTGGACCCCATGGGCACCCAGCACCACT‑TGCACGATCGCCATCAAC‑AACGCCCACAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTa < 2:336101/232‑1 (MQ=255) agGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGACCAGTATGCGCTAAATAACTCACAGTAGTGAGTATCGTTGTATTTTAGATGAGTGGAGAG‑ATAGAGAATCAGCCGGGCCCGCATACCGTAAAAGca > 1:123165/1‑238 (MQ=255) agGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCt > 2:262410/1‑239 (MQ=255) gAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGCCGTGACATACACCAGCGAACAGTACGCGCTAAATACCTCGTAGGCGCGAGTACCGTTGCATTTTAGTTGATTGGTGAG‑ATAGAGAATCACCAGGGCACGCATGCCGTaaaacttaa > 1:158948/1‑234 (MQ=255) cGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAaaat > 2:96285/1‑238 (MQ=255) gatggatCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATAAACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTTGATTTTAGTTGTTTGG‑GAGAATAGGGAATCAGCAGGGCCCCCATGCCGAAAAAATAAAAAAATTcc > 1:67699/1‑239 (MQ=255) | CCAGGCCAAAGCCCATCGCCCAGCTGTACTCTTCCTGGGCGTAACCACAGGCGATAGGTGCGATAATAGACCCCACGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTT‑GGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGGAATGACGGATGGATCTCACTGGCACCCAGCACCACA‑TGACCGATCGCCATCAAC‑AACGCCCCCAGCATCACCGCCATGCGATTGCCGAGAACTTTATCCGCCAAAAAGCCACCGAGGATTGGCGTGACATACACCAGCGAACAGTAGGCGCTAAATAACTCGTAGGCGTGAGTATCGTTGTATTTTAGTTGATTGGTGAG‑ATAGAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCC > NC_000913/742037‑742497 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |