| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 743,686 | C→G | 34.3% | R32G (CGA→GGA) | ybgJ → | putative allophanate hydrolase, subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 743,686 | 0 | C | G | 34.3% | 28.8 / 4.0 | 29 | R32G (CGA→GGA) | ybgJ | putative allophanate hydrolase, subunit 1 |
| Reads supporting (aligned to +/- strand): ref base C (15/3); new base G (8/2); total (24/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCAT > NC_000913/743459‑743916 | tgctgcAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGAAGTGCTGGG‑ACTGGAACCGCCGGGGACGCTGGACAGCCAGAAACGGA‑TGTGGGGACTTgcggcg > 2:875990/1‑236 (MQ=255) ctgcAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGCAGCGCTTGA‑ACTGGAACAGCCGGTGACGCGGGCTAACCAAGAACCGA‑TCTGGCggcgagcgccacg > 2:661841/1‑227 (MQ=255) cAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGG‑ACTGGAACCCCCGGTGACGCTGGCTAGCCAGAAACGGA‑GCTGGGGACTgggccgcgggcc > 2:239081/1‑229 (MQ=255) cAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGGGCTGGA‑ACTGGAACCCCCGGTGCCGCGGGCTAGCCCGAAACGGA‑TCTGGGGACTGgcgccgggccc > 2:295174/1‑232 (MQ=255) cACTGAACGTGGTGGTATTCGCGCATTGAGCGAGCGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGGGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGGGCTGGACAGTGGAAGC‑CCGGTGACGCTGGCTAGCCGGAAACAGA‑CCTGGGGCCGGGCGCAACgccggggggggaggcgg > 1:490127/1‑223 (MQ=255) aaTACCGATCTTGATGAGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGACAGGCTGGG‑ACTGGAACCCCCCGGGACGATGGCAAGCCAGAAACGGACTATGG‑GGCTGGCGCACCGTCCGGTGGTGAGTCCCGCA‑GAGGTGGAAGCCCTTCCCGGCAGGAAAAATAACaacgagagtt > 1:389246/1‑230 (MQ=255) gATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGGGGGTA‑TGCCGCATGGGGTTGAAGCCATTCCCGGCATGAACAATATcttgctcttttacacatctccg > 2:744383/1‑217 (MQ=255) aCCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGG‑ACTGGAACCGCCGGGGACGCTGGCTAGCCAGAAACGGA‑TCTGGGGACTGGCGCAGCGTCTGGTGGATA‑TGCCGCATTTGGTTGAAGGCATTTCCGGCAGGAAAAATATCACGGGGGTTTTGCGGAAACCttgtgcgc > 2:793426/1‑232 (MQ=255) aCCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTTGCCAGAAACGGA‑TCTGGCGACTGGCGCCGCGGCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATACCGGGGATTTTGCGTAATCCTTAGT‑cgc > 2:453715/1‑239 (MQ=255) aCCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGAAGCTTGCTAGCCAGAAACGGA‑TCTGGGGACTGGCGCAGCGGCTGGTGGATA‑TGCCGAATTTGGTTGAAGCCATTCCCGGCATGACAAATATTACGGTGGTTTTGCGTAATCCTGAGT‑c > 2:847480/1‑237 (MQ=255) aCCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGAATTGCTGTG‑AATGGAACCGCCGGTGACGCGGGCTAGCCAGACAAGGG‑TGTGGCGACCGGGGCCGCGGCCGGTGGGAA‑TTCCGAATAGGGTTGAAGCCAATCCCGGCATGAACCATTATACGGCGGTTTTTCGGAATACTAGGT‑cgc > 1:526916/1‑239 (MQ=255) atatTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGGGACGCTGGCTAGCCAGAAAAGGA‑TCTGGCGACTGGCGCCGCGTCTGGTGGGTA‑TGCCGAAATTGGTTGAAGCCATTCCCGGCATGAAAAAAATCACGGGGGTTTTGCGGAATCCGGAggtcccggggccggg > 2:238483/1‑225 (MQ=255) ttCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGGCTGGCGCAGCGGCTGGTGGGTA‑TTCCGAATGTGGTTTAAGGCATTCCCGGCATGGACAATATCACGGGGATTTTGCGGAATCCTAGGT‑cgcttggctcgggtg > 2:27711/1‑227 (MQ=255) cATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGG‑ACTGGAACCGCCGGTGACGCCGGCTAGCCAGAAACAGG‑ACTGGGGCCCGGCGCAGCGGCTGGTGGATA‑TTCCGCATGTGGTTGGAGGCATTCCCGGCATGAACAAAATCACGGGGATTTTTCGTAAACCTGGGTGCACGGGC‑CGGGATGCCATagagaggtt > 1:57622/1‑234 (MQ=255) cATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGG‑AAAGGAACCGCCGGTGACCCTGGCCAGCCCGGAACGGG‑TCAGGGGACTGGCGGCGCCGCCGGGGGATA‑TGCCGCAAGGGGTTGGAGCCCTTCCCGCCCTGGCAAATATCACAGGGCTTTTTGGTAACCCTCGGT‑cgcggggcgcggagtacaacaagagttt > 1:409753/1‑217 (MQ=255) cATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAACCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGGGATTTTGCGTAATCCTGAGG‑CCCTGGCGCTGGATGCCCTAGAGGGttt > 2:541094/1‑239 (MQ=255) aTAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGGGACGCTGGCTACCCAGAAACGGG‑TCTGGCGGCTTGCGCCGCGTCTGGTGGGTA‑TGCCGAAAGTGGGTTAAGCCCTTCCCGGCATTAAAAATATCACCGGGGTTTTTCGTAATCCTCAGT‑CGCTGGGGCTGGGTGcccaaaaggggttg > 1:713151/1‑227 (MQ=255) ggTGAAACGGCGGAAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGCATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGGGTAATCCTGAGG‑CGCTGGCGCTGGATGCCCTAGAGCGCTTTCTACACTGCTGGGGGGGGGCCGAGGCGCGGGAGCCGGAgggtcggtgtctgta > 1:784394/1‑225 (MQ=255) aCGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCCAGCCAGAAAAGGA‑TCTGGCGGCTGGCGCCGCGTCTGGGGGATA‑TGCCGGAAGTGGTTGAAACCCTTCCCGGCCTGGACAAAAATACGGTGGTTTTGGTTAATACTGCGG‑CGCTGGGGCGTGGTTCCATAGAGCGTTTGAGACGCTGGGGGGGGGAGGAGGGGGGGGGGGGGCCGCGGTCTCGCTTTATTTaaaagtc > 1:517608/1‑235 (MQ=255) ggtggaggtgcgggagtgtgacccgccgGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCgg < 2:558643/217‑1 (MQ=255) ggggtaggggagaaaggcgcacCGCCGGTGACGGTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGGCGAATGTTGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCgg < 2:466757/218‑1 (MQ=255) gTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGAGTTTGCAACGCTGGGGGGAGGCGAGCGGGGCGCGGGAGCCGGAGTCTCGCTTTTTTGAAATTCCgggggt > 2:801144/1‑239 (MQ=255) tatgagagtccccgccgGTGACTGTGGCTGGCGAGAAACGGA‑TTTGGGCATTGGGGGAGCGTCTGGTGGATT‑GGCCGAATGTGGTTGAAGCCAATCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGGTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACgg < 1:875990/227‑1 (MQ=255) actggaacCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCCGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGACCAATATCACGGTGATTTTGCGTAATCCTGAGC‑CGCTGGCGCTGGAGGCCATCGAGCGTTTGCAACGCTGGTGGGTGGAGAGCGAGGGGCTGGAGCCGGAGTCCCGCTTTCTTGAAATCCCGGGGGtttcaggaggg > 1:161457/1‑230 (MQ=255) cgccgGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGCGAGCGAGGCGCTGGGGCCGGAGTCTCCCTTTATTACAATTCCGGTGGGTTCTGGTGGTGCAGGCgg > 2:367581/1‑238 (MQ=255) ccgGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGGGGTTTACGGTGGTGCAGGCGGAcc > 2:492820/1‑239 (MQ=255) cTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGCAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGGGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGAGGGTTACGGGGGGGCAGGCGGACCGGATTTGGc > 2:97899/1‑239 (MQ=255) ggcgaggcaagaaCGGT‑TTTGGGGACGGGGGCAGGGGTGGGTGGATA‑TGTCGAATGTGGTTGAAGCCATTCCCGGCATGAACAAGATCACGGTGAGTTGGCGTAATCCTGAGG‑CGCTGGCGCTGGATGCCAGAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCg < 1:367581/227‑1 (MQ=255) ggA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGGTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCAt < 2:80368/239‑1 (MQ=255) | TGCTGCAGGTCACCATGCCACTGAACGTGGTGGTATTCGCGCATTGAGCGAGTGGCTGAATGAAAATACCGATCTTGATGTGACCTTTATTGATATTCCTAATCCTGCATAACGAATAATCAGAGGGATCGAAAGTGCAACGAGCGCGTTGTTATCTGATAGGTGAAACGGCGGTAGTGCTGGA‑ACTGGAACCGCCGGTGACGCTGGCTAGCCAGAAACGGA‑TCTGGCGACTGGCGCAGCGTCTGGTGGATA‑TGCCGAATGTGGTTGAAGCCATTCCCGGCATGAACAATATCACGGTGATTTTGCGTAATCCTGAGT‑CGCTGGCGCTGGATGCCATAGAGCGTTTGCAACGCTGGTGGGAGGAGAGCGAGGCGCTGGAGCCGGAGTCTCGCTTTATTGAAATTCCGGTGGTTTACGGTGGTGCAGGCGGACCGGATTTGGCGGTGGTCGCGGCGCAT > NC_000913/743459‑743916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |