| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 842,815 | C→A | 17.2% | Q162K (CAA→AAA) | rlmF → | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 842,815 | 0 | C | A | 17.2% | 140.9 / 2.2 | 59 | Q162K (CAA→AAA) | rlmF | 23S rRNA m(6)A1618 methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (26/22); new base A (2/9); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCAAAAAGATGATTGAAGAGAGCA > NC_000913/842578‑843052 | cgggcGGATTTATTTCATCCCCTTGCCGATTTCCTGGCAGAAGCAAGCGGAAAAATTCCAGCGAATGCCATCATTATGGATACTGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGGGTTTTA‑CCGGTAGTGAAACCAGCCGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGc < 1:804806/238‑1 (MQ=255) ggcGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCaaa > 2:279598/1‑239 (MQ=255) ggcGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCCCCATTGGTCT‑GCGTCGGCaaa > 2:166844/1‑239 (MQ=255) tcatccCCTTGCCTATTTACTGGCAGAAGCAAGGGGAACAATTCCCGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTgggg < 1:269627/239‑1 (MQ=255) tcaacaCCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTgggg < 2:264163/239‑1 (MQ=255) tcaCCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGACTGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATACGCTGATTGGCGTACATGACTATGGCTGGAGTTTTA‑CCGGTAGGGAAGACAGCGGCCCGGGGTTAAGCAGATGGCAGGCGATTTTCGGATATAAACCAGGGCATAACAGGCCCATTCGGCT‑GCGGCGGAACAAAGAGAGGGGGG‑CGa > 1:801309/1‑239 (MQ=255) tcaCCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGGGCAGGGGGTTATCAGTTCTAATCCGGGGTTTAACACCGCCATTC‑TCTGGCTGCGGCaaaaaaaaaaggggggga > 1:136163/1‑226 (MQ=255) aCCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGGGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGGCCCATTCGTCT‑GCGTCGGCAAAAAAAGAGTGGGG‑CGAtt > 2:678892/1‑239 (MQ=255) tGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGGGTTAAGCAGTGCGCAGGCGCTTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGAAAAAAGAGAGGGGGG‑CGAGTTTTa > 1:582767/1‑239 (MQ=255) tgcgttttCCTGGCAGAAGGAAGGGGAACAATTCCAGCGAATGCCAGCATTCTGGAGAGCGGTGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCGGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTaa < 1:861553/234‑1 (MQ=255) gCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTaa < 2:582767/239‑1 (MQ=255) agcaagcGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGTTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATaaaa < 2:632373/239‑1 (MQ=255) aagcGGGACAATTCCAGCGAATGCCAGCATTCGGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAAc < 2:898591/238‑1 (MQ=255) aaatggAACAATTCCAGCGAATTCCCGCATTCTGGATTGCTGCTTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGGCAGGTCTTAAGCAGTGGTCAGGCAATTATCAGTTCTAATACGGGGCTTAACCGCGCCATTCGTAT‑GCGTCCGCAAAAAGAGAGCGGGG‑CGATTTTTAACGGCATCATCCATAAAAACg < 1:678892/235‑1 (MQ=255) ggAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAg < 2:199260/237‑1 (MQ=255) ggAAAAATTCCAGGGAATGCCAGGATTTGGGAAATCGGGGTTGGTGCAAACTGTATGTAACCGCTGATTGGTGTACATGAATATGGGTGGCGTTTTA‑CCGGTAGGGAAACCAGCAGCCAGGCGTTAAGCAGGTCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAg < 1:529862/237‑1 (MQ=255) aaCAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACGTTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑Caa < 2:440757/238‑1 (MQ=255) ttCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATacgac < 2:346443/239‑1 (MQ=255) ttcaggcaaTGCCAGTATTCTGGATTTCGGGTTGGGGGCGAACTGTATTTATCCGCTGAGTGGCGTACATGAATATGGCTGGCGTTTCA‑CCGGTAGTGAACCCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATacgacg < 1:279598/232‑1 (MQ=255) tCCAGCGAATGCCAGCATTCTGGATATCGGCGTTTGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATacgacg < 2:676534/239‑1 (MQ=255) gggAATGCCAGCATGTTGGATATCCGGGTTTGTGCGAACTGTATTTATCCGATGATTGGCCGACATGAATCTGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCAGCCATAAAAACGAG‑CAATACGAcgc‑gtc < 2:66707/237‑3 (MQ=255) tagaagacAGTATTGTGGATATTGGTTGTGGTGGCAACTGTATTTATCCGCTGGTTGGCGTACGTGAATATTGCTGGCGTGTGA‑AGTGTAGTAAACACAGCAGGCAGGCGTTAAGCAGTGTGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTGT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCAGCCATAAAAACGAG‑CAATACGACGC‑Gccc < 1:855057/232‑1 (MQ=255) tGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTCTCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGAGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGTGCCATTCGTCT‑GCGTCGGCAAAAAGGGGGTGGGG‑CGATTTTTAACGGCATCTACCCTAAAAACGAC‑CAAAacaaacggcccttgg > 1:860176/1‑226 (MQ=255) tGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCCTAAAAACGAG‑CAATACGACCC‑GACCTtgt > 2:566416/1‑239 (MQ=255) ctgttttctgtttttCGGCTGTGGTGCGAATTGATTTTACCGGGTGTTTGGCGTCAATGAATGTGTGTGGGGTTTTC‑CCGGTAGTGAAACAAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGTTTACCAGTTCTAACCCGGGGCTAAACCGCGCTATTCGTCG‑GCGTCGGCAAAAAGAGAGTGGGG‑CGTTTTTTAACGGCATCACCCAAAAAAACGAG‑CAATACGCCGC‑GACCTTGTGT‑a < 1:325393/225‑1 (MQ=255) cATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCCAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AAc > 2:208403/1‑238 (MQ=255) cGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATACCCCATAAAAACGGG‑CAAAACGACCC‑GACCTTTGGTAAACCCCCCATccccc > 1:387804/1‑237 (MQ=255) cGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAAGCGTTAAGCAGTGCGCAGGCGGTTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGC‑GGCTTTTTAACGGCATCCTCCATAAAAACGGG‑CACAACGCCGC‑GCCCTTGGGT‑AACCGCCCATTCCAca > 1:490404/1‑238 (MQ=255) gtacttttttttttcggttgtttGGCGTAAATG‑ATATGGGTGGGGTTTTACCCGGTAGTGAAACCCGCCGCCGGGCGTAAAGCGGGGCGAGGGGGTTTATCATTTTTATTCCGGGGCTTAACCGCCCCATTCGTCG‑GCGTCGGCAAAAAGAGGGGGGGG‑CGTTTTTTAACGGCATCACCCCAAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCt < 1:713585/218‑1 (MQ=255) ttATTCATCCGCTGTGTGGGATCCTGGAATTTGGGTGGTTTTTTA‑CCGGTAGGGAAACACGCAGCCGTGCTGTAAGCGGTGCGCAGGCGATTACAAGTTCTAACCGGGGGCTTAACCGCGCAATTCGTCC‑GCTTCGGCAAAAGGAGGGTGGGG‑CAATTTTTAACGGCATAATCCATAAAAACGAG‑CAATACGAGGC‑GCCCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCa < 1:771061/238‑1 (MQ=255) tgttttACCCGGTTATTGGCTTCAGTGAATTTGGTTGGCTTTTGA‑CCGGTAGGAAAACCAGCAGCCGGGCTTTAAGCAGGGCCAAGGCGATTACCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCa < 2:136163/236‑1 (MQ=255) tATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACCC‑GACCTTGTGG‑AACCCGCCATTCCCCGATTCCGCCGCTGCGGccc > 2:635164/1‑237 (MQ=255) cTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTCATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCCAAAAGAGCGGGGGG‑CGATTTTTAACGGCATACTCCCTAAAACACAG‑CAAAACGAACC‑GACCCTTGGT‑AACCCACCCTTCCCAAATTCCGCCGCTGCggacgcggccgggaa > 1:58866/1‑226 (MQ=255) cTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATaaaa < 2:437991/169‑1 (MQ=255) cTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATaaaa > 1:437991/1‑169 (MQ=255) cTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACCC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCCCCCCGCCGGCACGGGCAgggaa > 1:519236/1‑236 (MQ=255) cTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGGGGGG‑CGATTTTTAACGGCCTCATCCGTAAAAACGAG‑CAA‑AAGACCCGGCCCTTGTGT‑AACCCGCCATTCCCACATTCccccgcccgggaccgggggggagg > 1:788222/1‑221 (MQ=255) gATTGGCGTACAGGAATATGGCGGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCGGGCTTTAAGCAGGGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGAAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑ACCCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTg < 2:244312/239‑1 (MQ=255) ggggggggtttttA‑CGGGTAGTAAACCCGGCCGCGGGGCGTTAAACAGTGCGCAGGGGATTACCAGTTTAAACCGGGGGCTAACCCGCGCCATTCGGTG‑GGGGGGGAAAAAAGAGAGGGGGG‑CGATTTTTAACGCCATCACCCATAAAAACGGG‑CAATCCGCCGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAAcc < 1:566416/230‑1 (MQ=255) ctggcGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCCGGTAGTGAGCGGAAAAGCCGTAACCTgg > 2:722284/1‑239 (MQ=255) ggcGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTAGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCCCTGGGGCACGGGCAGGAAGTGAGCGTAAACGCCGTAACCCgggg > 1:67216/1‑239 (MQ=255) gcGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGGGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACCC‑GCCCTTGTGT‑AACCCGCCATTCCCCGCTTCCGCCCCTCCGGCCCGGGCAGGTAGGAGGCGAAAAACCCGGAACCCggggg > 1:171736/1‑238 (MQ=255) gcGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGAAGTGAGCGTAAACGCCGTAACCTggg > 2:548728/1‑237 (MQ=255) cccgggtggaaaaCCAGGAGCCGGGGTTTAAGCAGGGCGCGGGGGATTAGCAGTTCAAAGCGGGGGCTTACCCGCGCCAGTCGTCG‑GCGTGGGAAAAAAGAGAGGGGGG‑CGATTTTTAACGCCACCATCCAAAAAAACGAG‑CAATCCGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACaa < 1:296114/229‑1 (MQ=255) aaaCCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCCCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGAcattgc > 2:634171/1‑233 (MQ=255) gTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGAAGATGCACTGAACTTTGGCGGCCAGCAACAg > 2:443077/1‑239 (MQ=255) tGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTCAGCGCAAACGCCGGAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCACCAACAgg > 2:227239/1‑239 (MQ=255) gcAGGCTATTATCAGTTCAAAGCCGGGGTTTAACCGCGCCATTCGGCT‑GCGTCGGAAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCACCCATAAAACCGAG‑CAATACGACGC‑GCCCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAg < 2:171736/238‑1 (MQ=255) ggCGATTAACAGTTCTAATCCGGGTCTTAACCGGGCCATTCGTCT‑GCGTCGGAAAAAAGAGAGGGGGG‑CGATTTTAAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTtg < 2:591993/238‑1 (MQ=255) aTCAGTTATAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGACGGAAAAAAGAGAGGGGGG‑CAATTTTTAACGGCATCACCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGa < 2:387804/239‑1 (MQ=255) gTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCACCAACAGGGGTTGTGGTGTGAAGGc > 2:420660/1‑239 (MQ=255) tCTAAGCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGGGTGGGG‑CGATTTTTAACGGCATCATCAATAAAAACGAG‑CAATCGGCCGG‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCg < 2:490404/238‑1 (MQ=255) aTCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGGAGTGAGCGTAAACGCCGTAACCTGGGGCTGGACAAAGACGCTGCACTGAACTTTGGCGCCCAGCAACAGGAGGGGTGGGGTGAAGACGGGGag > 1:583099/1‑238 (MQ=255) gggCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACCAAAACGATGACCTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGGGTGAAGGCGGTGAAGTc > 2:17965/1‑237 (MQ=255) gcCATTCGTTG‑GCTTCGGCAAAAAGAGAGGGGGG‑CTATTTTTAACGGCATCATCCAAAAAAACGAG‑CAATGCGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCaaaaa < 2:712672/239‑1 (MQ=255) gcCATTCGTCG‑GGGGCGGAAAAAAGGGAGTAGGG‑CGATTTTAAACGGCATCACCCAAAAAAACGAG‑CAATACGGCGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCaaaaa < 2:67216/239‑1 (MQ=255) t‑GCGCCGGAAAAAAG‑GAGTGGGGTCTTTTTTTAACGGATCCCTCAAAAAAAGCGAG‑CATAGCGGCGC‑GCCCTTGGGA‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCAAAAAGATGATTGaa < 1:208403/239‑1 (MQ=255) gctgcGGAAAAAGGAGAGTGGGG‑CGATTTTAAACGGCATAATCCA‑AAAAACGGGACAATCCGCCGG‑GCCCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCAAAAAGATGATTGAag < 2:70083/235‑1 (MQ=255) cGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTTTCAAAAAGATGATTGAagg > 2:619345/1‑238 (MQ=255) gCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGGAGTGCGCGTAAACACCGTAACCTGGGGCCAAACAAAGACAAGGCCCGGACCTTTTGCGGCCACAAAAAGGGGATGTGTGGGGACGGAGGGGAGGTCACCTGTTTCAAAAACAGAATTGaaagaagaa > 1:881792/1‑232 (MQ=255) | CGGGCGGATTATATTCATCACCTTGCCGATTTACTGGCAGAAGCAAGCGGAACAATTCCAGCGAATGCCAGCATTCTGGATATCGGCGTTGGTGCGAACTGTATTTATCCGCTGATTGGCGTACATGAATATGGCTGGCGTTTTA‑CCGGTAGTGAAACCAGCAGCCAGGCGTTAAGCAGTGCGCAGGCGATTATCAGTTCTAATCCGGGGCTTAACCGCGCCATTCGTCT‑GCGTCGGCAAAAAGAGAGTGGGG‑CGATTTTTAACGGCATCATCCATAAAAACGAG‑CAATACGACGC‑GACCTTGTGT‑AACCCGCCATTCCACGATTCCGCCGCTGCGGCACGGGCAGGTAGTGAGCGTAAACGCCGTAACCTGGGGCTGAACAAAGACGATGCACTGAACTTTGGCGGCCAGCAACAGGAGTTGTGGTGTGAAGGCGGTGAAGTCACCTTTATCAAAAAGATGATTGAAGAGAGCA > NC_000913/842578‑843052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |