Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 846,743 G→C 13.4% A126G (GCG→GGG)  glnP ← glutamine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913846,7430GC13.4% 176.9 / 3.2 77A126G (GCG→GGG) glnPglutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base C (6/6);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCA  >  NC_000913/846506‑846975
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         
aGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTGGAGAATGGTTTCCCAACGTGAAAGACCGAgcg                                                                                                                                                                                                                                           >  2:754318/1‑238 (MQ=255)
               accgaaTAGACCTCCGCCCTGGCGCTCCAGATCTCAAGGGCCGGGAAGTTTCCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAATCAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACg                                                                                                                                                                                                                           <  2:252738/234‑1 (MQ=255)
                  aGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGCCGTAGGGAATGGTTTCCCAACGTGAAAAACCAAGCCCCAGCCCTGCTTCaacaaa                                                                                                                                                                                                                        >  1:132722/1‑234 (MQ=255)
                  aGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGGGCCAGCGGTAAAATGACGCAGCGAATGGTTTCCCAACGTGAAAAACCGAGCGACAGCCCTGCTTCACGaaa                                                                                                                                                                                                                        >  1:377434/1‑239 (MQ=255)
                   gATAAAACCCCGCCACGGCGCCCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGaaa                                                                                                                                                                                                                        <  2:102857/238‑1 (MQ=255)
                    aTAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACCATAAACAGCGAGGAGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGATTCCCACCGCGAAAGACCGAGCGACAGTCCTGCTTCACGaaaa                                                                                                                                                                                                                       >  2:66234/1‑238 (MQ=255)
                    aTAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATAAGACGCAGTGCCAGCGGGAAAATGACGTAGCGAATGGGTTCCCACAGTGCAAAAACGAGGCGCACGCCTGGTTCACGcaaa                                                                                                                                                                                                                       >  1:57223/1‑238 (MQ=255)
                       gAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACCCAGTGCCCGCGGTAAAATGACGGAGCGAAAGGTTTCCCAACGTGATAAACCGGCCCCCAGTCCTGCTTCACGAAAACCtt                                                                                                                                                                                                                   >  2:750038/1‑239 (MQ=255)
                        aaCACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGCCACCATACGCCCCCGTGCCAGCGGTAAAATTACGTAGCGAATGGTTTCCCAACTCGAAAGACCCACCCCCAGTCCTGCTTCCCGAAACACttt                                                                                                                                                                                                                  >  1:442546/1‑239 (MQ=255)
                            cccccACGGGCCTCCAGATCTCAAGGGCGCGGAATTTACCGGCAATAAGTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTgg                                                                                                                                                                                                              <  1:401914/236‑1 (MQ=255)
                              gCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGCCCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGAt                                                                                                                                                                                                            >  2:623162/1‑239 (MQ=255)
                              gCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGGAAAATGACGTAGCGAATGGTTTCCCAACGGGAAAGACCGAGCGCCAGTCCGGCTTCACGAAAAACTTTGTGGaa                                                                                                                                                                                                            >  1:172931/1‑238 (MQ=255)
                                 ccgccgcCCCAGATTCCAAGGGCGGGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGa                                                                                                                                                                                                         <  1:844933/235‑1 (MQ=255)
                                 ccgccccccccgttctcTAGGGGGCCGAAGTTCCCGGAAATATGTTCCTGCCCCTGACGGGTCTGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGa                                                                                                                                                                                                         <  1:750038/226‑1 (MQ=255)
                                       cTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGGGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGGGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCCCGAAAACCTTTGTGGGTAGACAcca                                                                                                                                                                                                   >  2:89565/1‑238 (MQ=255)
                                            ggtctcAGGGGCGCGGAAGTTCCCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAgcaccgcac                                                                                                                                                                                             <  1:106935/237‑1 (MQ=255)
                                            gATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGGCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAgcaccgcac                                                                                                                                                                                             <  1:106957/239‑1 (MQ=255)
                                               ctcAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGATCGCCAGTCCTGCTTCACGAAAACCTTTGTGGAAAGACAGCACCGaacca                                                                                                                                                                                           >  2:728016/1‑238 (MQ=255)
                                                  aaGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAATCCTGCTTCACGGAAAACTTTGTGGATAAACAAACCCGCACCAcccg                                                                                                                                                                                       >  1:106124/1‑239 (MQ=255)
                                                  aaGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGGTTCCCAACGGGAAAGACCGGGGGCCAGTCCTGCTTCCCGAAAAACTTTTTGGATaaaaaaacccacaccccccg                                                                                                                                                                                        >  1:443566/1‑220 (MQ=255)
                                                       cgcgGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATTACGTAGCGAATGGTTTCCCAACGGGAAAGACCCAGCGCCAAGCCTCCCTCCCGAAAAACTTTGTGGATAAACAGACCCCcaccaccccggat                                                                                                                                                                                   >  1:39440/1‑231 (MQ=255)
                                                       cgcgGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCCCCAGTCCTGCTTCACGAAAACCTTTTTGGATAGACAGAACCGCACCACCCGTGAtt                                                                                                                                                                                  >  2:507811/1‑239 (MQ=255)
                                                       cgcgGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCCAGCGCCAGTCCTGCTTCACGAAAAACTTTGTGGATAGACAGAAACACACCCCCCGTGAtt                                                                                                                                                                                  >  1:678575/1‑239 (MQ=255)
                                                       cgcgGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGATTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGAtt                                                                                                                                                                                  >  2:431905/1‑239 (MQ=255)
                                                       cgcgGAAGTTACCGGAAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGAt                                                                                                                                                                                   <  2:240600/238‑1 (MQ=255)
                                                         cgGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGATTCCCAACGTGAAAGACCGAGCGCCAGCACTGCTTCACGAAAACCTTTGTGGATAGACAGAAACGCCCCCCCCGTGAttt                                                                                                                                                                                 >  2:344054/1‑238 (MQ=255)
                                                         cgGAAGTTACAGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGCATCCGGTCCACCGGTAAAATAACGTTGCGCATGGTGTCCCAACGTGAAAGACCGTGACCCAGCCCCGATTCACGAACAACTTTGTGGAAAGACAccaacgccaccaacggagttt                                                                                                                                                                                 >  1:714378/1‑224 (MQ=255)
                                                          ggAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTc                                                                                                                                                                                <  2:39440/238‑1 (MQ=255)
                                                          ggAAGTCACCGGCAATACTTTCCTGCCCCGTACGGGTCAGTTCCGACACGTCCATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTc                                                                                                                                                                                <  2:282977/238‑1 (MQ=255)
                                                                  ccGGCAATAATTTCTTGCCCCGGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAAtata                                                                                                                                                                       <  2:514059/239‑1 (MQ=255)
                                                                       cacaagtgctggCCCCGGGCGGGTCAGCTCCGCCCCGCGCATACAAATAAACGGCGAGGTTTCTTTAATGCCGTGGGTCCACTGGTTCACCAGCGGCGGCAGCATACGCCGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGAGAAAGACCTAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCTAGCGTGAGTTCCTCCATATACgtgc                                                                                                                                                                  <  1:496652/228‑1 (MQ=255)
                                                                       aataatTTCTTGCCCCTGACGGGTCAGTTCCGCCAAGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACgcgc                                                                                                                                                                  <  2:97803/239‑1 (MQ=255)
                                                                        ataatTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACgcgc                                                                                                                                                                  <  2:377434/238‑1 (MQ=255)
                                                                        aaaatTTCTTGCCCTGGAAGGTTCAGTTCCGACCCGGCGATCCAAAAAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCCGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACgcgc                                                                                                                                                                  <  1:28707/236‑1 (MQ=255)
                                                                            tttcttctctcttacggttttgTTCCGCCCCGCCCAACACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAg                                                                                                                                                             <  1:398000/218‑1 (MQ=255)
                                                                            tttCGTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAg                                                                                                                                                             <  2:252866/239‑1 (MQ=255)
                                                                             ttCTTGCCCCTGACGGGTCAGTTCCCACACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGt                                                                                                                                                            <  2:57223/239‑1 (MQ=255)
                                                                              tCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGtt                                                                                                                                                           <  2:132722/239‑1 (MQ=255)
                                                                              tCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGtt                                                                                                                                                           <  2:751675/239‑1 (MQ=255)
                                                                                  gCCCCTGACGGGTCAGTTCCGCTACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCGCTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATc                                                                                                                                                       <  2:896491/239‑1 (MQ=255)
                                                                                   ccccctccgggcacttttcGCCCCGCCGATCACAATAAACAGCGAGGTGTTTTTAATGCTGTTGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCa                                                                                                                                                      <  1:817453/221‑1 (MQ=255)
                                                                                     ctggACGGGTCAGTTCCGCCCCGCCGATCACAATAAACAGGCAGGGGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGGAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCatg                                                                                                                                                    <  2:125358/236‑1 (MQ=255)
                                                                                              tCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAACACCGCACCACGCGTGATTTCCGCCAAATAAGCGCCGGAGTCGAGCaaataggggacc                                                                                                                                           >  1:748109/1‑228 (MQ=255)
                                                                                                aGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCACTATACGCCCCGGAGTTGGTCATGATGGGGacac                                                                                                                                          >  2:328560/1‑236 (MQ=255)
                                                                                                                 cacaACAAACAGCGAGGTGTCTTTAAGGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTCCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAACCCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGt                                                                                                                        <  2:167823/239‑1 (MQ=255)
                                                                                                                    aaTAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACCCGTGATTTCCGCAAAATCAGCCCCGGGGTTGATCATGATGGGTACCACCCCCGCAGaaaaatgggcga                                                                                                                     >  1:774601/1‑231 (MQ=255)
                                                                                                                     ccagacaggcaGGTGCCTTTAATCCGTATGAACCACGGGTTACCCAGCGGCGGCAGCATCCGACCCAGTGCCAGCGGTAAAATGACGTAGCAAATGGTTTCCCAACGGGAAAGCCCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCg                                                                                                                      <  2:118485/227‑1 (MQ=255)
                                                                                                                           aGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATgggcggaagcgaaa                                                                                                              >  2:298464/1‑228 (MQ=255)
                                                                                                                                  tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGGCCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTgg                                                                                                                                                                                                              <  2:846825/137‑1 (MQ=255)
                                                                                                                                  tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGGCCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTgg                                                                                                                                                                                                              >  1:846825/1‑137 (MQ=255)
                                                                                                                                  tgtCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCCGTAAATGGGTCGATACGTAAGTCGTTa                                                                                                       >  2:496132/1‑239 (MQ=255)
                                                                                                                                    tCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGACCCGCACCACGCGGGATTTCCGCAAAATACGCGCCGGAGTTGATCCTGATGGTGACCACCCCCCCGGGAAATGGGCCGAGGGGTAAgacctgc                                                                                                       >  1:118485/1‑231 (MQ=255)
                                                                                                                                              tgatgaTCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGAGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCCAGCGCCAGTCCTGCTTCACGAAAACCTTTGGGGATAGACATCACCGACCCCCGCGTGTTTTCCGCACTATACGCACCGCAGTTGACAATGAAGGGGACCACCCCGTCCGAAAATGGGCCGAGCCGTATGACGTAAAAAGCCacggga                                                                                           >  1:880637/1‑234 (MQ=255)
                                                                                                                                              ggattgccctggggTTCCCCAGGGGGGGCTGCATACGAGGCAGGGCCAGGGATAAAATGCGGTACGAAATTGTTTCCCAACGGGAAAGCCCGAGCCCAAGGCCGGCTTCACGAAAACCTTTGGGGATAGACAGCCCCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCgg                                                                                            <  1:507811/226‑1 (MQ=255)
                                                                                                                                                atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCCCGCGGGATTTCCGCAATATACGCGCCGGGGGTGATCATGAAGGGGACCACCCCCGCAGTAAAGGGGGCGAGGGGAAAGTCGTAAAAAGCCAACGGCAg                                                                                         >  1:499976/1‑239 (MQ=255)
                                                                                                                                                atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAg                                                                                         >  2:268221/1‑239 (MQ=255)
                                                                                                                                                atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAg                                                                                         >  2:576315/1‑239 (MQ=255)
                                                                                                                                                atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCAACGCACCACGCGTGATTTCCCCAATATACGCGCCGGAGTTGATCATGATGGTGACCAACGCCCCAGAAAAGGGGTCGAAGCGGAAGAGGTTAAACGCCCACGGCAg                                                                                         >  1:723937/1‑239 (MQ=255)
                                                                                                                                                atgatCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGAAAGCACCGCACCACGCGGGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACACCCGCCCCAGGAAAAGGGGCGAGGGGAAAATAGTAAAAAGCCACCGGGAg                                                                                         >  1:400779/1‑239 (MQ=255)
                                                                                                                                                      cACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCAAACGTGAAAGCCCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGaa                                                                                   <  2:443566/239‑1 (MQ=255)
                                                                                                                                                             tccccAGGGGCGGCAGCATCCGGCCCAGTCCCAGCTGTAAAATGGCGTAGGTAATGGTTACCCAACGTGAAAGACCGAGCCCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCCCCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGaaataaat                                                                             <  2:499976/236‑1 (MQ=255)
                                                                                                                                                                   gcggcgGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGCTAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACAACCGCCGCAGGAAATGGGTCGATGCGTAAGTGCATAAACGCCATCGGCCGGGGGAAAAAAAAAAACacc                                                                      >  1:605568/1‑237 (MQ=255)
                                                                                                                                                                     ggcggcAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCCATATACGCGCCGGCGTTGATCATGATGGGGACCACCGCCGCAGGAAATGGGTCGATGCGTAAGCCGTTAAAGCCCACCGGCAGGGCGAAAAAAAAAAACAtac                                                                     >  1:426305/1‑236 (MQ=255)
                                                                                                                                                                     ggcggcAGCATAAGACCTAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGTGCGCCAGTCCTGCTTCACGACAACCTTTGTGGATAGACAGCACCGCACCGCGCGTGATTTCCGCACTATAACCGCCGGAGTTGATCATTATGGGGACCAACGCCGCAGGAAAAGGGTCGCTGCGTAAGCCGTTAAAGGGCATCGGCAGGGGGCAAAAAACAAAAACCAc                                                                    >  1:765947/1‑239 (MQ=255)
                                                                                                                                                                            gcaTACGACGCAGGGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGCCCGAGCCCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCCCCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGgac                                                              <  2:748109/238‑1 (MQ=255)
                                                                                                                                                                              aTACGACGCAGGGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGgacg                                                             <  2:476537/237‑1 (MQ=255)
                                                                                                                                                                                         tGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGAAAGGtgtg                                                 >  2:854076/1‑238 (MQ=255)
                                                                                                                                                                                         tGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGAGGACCACGCCCGCCGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCCGGGCGAAATAAAAAAAAAATACCCGGGCGACGAAAggagggc                                                >  1:382299/1‑234 (MQ=255)
                                                                                                                                                                                                ggTAAAATGACGTAGGTAATGGTTTCCCACCGTGAAAGACCGGGCGCCAGCCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTAATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGAt                                         <  2:723937/239‑1 (MQ=255)
                                                                                                                                                                                                 gaaaaaTGACGTAGCCAATGGTTCCCCAACGTGAAAGCCCGAGCCCCAGTCCTGCTTCACGAAAACCTTTGGGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATCCGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGAt                                         <  2:106124/236‑1 (MQ=255)
                                                                                                                                                                                                 gTAAAATGACGTTGGAGATTTTTCCCCAGCGGAAAAGACCGCGGCCCAGCCCTGTTTCCAGAAACCCTTTGGGGAAAGACACACCCGCCCCCGGCGGTATTTCCGCAATATACCGGCCGGAGTTGATCATGATGGTGCCCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGAt                                         <  1:2389/238‑1 (MQ=255)
                                                                                                                                                                                                   aaaaTGCCGTAGCGAAGGTTTCCCAAACGGGAAAGCCCGGGCGCCAGTCCTGCTTCACGAAACCCTTTGGGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCa                                       <  2:400779/238‑1 (MQ=255)
                                                                                                                                                                                                     aattgcttgagaaaTGGTTCCCAAACGGAAAAGCCGGGGCCCCATTCCGGTTTACGAAAAACCTTTTGGAAAAGCAGCCACCCGACCTCGCGTTATTCCCGCAATAGCCGCGCCGGAGTTGATCAGGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACtt                                    <  1:702318/227‑1 (MQ=255)
                                                                                                                                                                                                     aaagcgtgggcgatgggTTTCCCAACAGTAAGACCGGGCCGCCAGTCCGGCTCAACAAAAACTTGGGGGGAAAGACAGCACCGCACCACGCGTGATTTCAGCAAGACGCGGGCCGGGGTTGACCATGATGGTGACCGCCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACtt                                    <  1:431905/224‑1 (MQ=255)
                                                                                                                                                                                                     aaTGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGTTGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGCACACGATTGGTGTGCCGCGGCACACtt                                    >  2:803485/1‑239 (MQ=255)
                                                                                                                                                                                                            tAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACCATAGGTGTGCCGCGGATCACTTCAATaaa                             >  2:530238/1‑239 (MQ=255)
                                                                                                                                                                                                                                 gaaaaGCCCGAGCGCCAGTCCGGCTTCACGAAACCCTTTGTGGATAGACAGCCCCGCACCACGCGTGATTTCCGAAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGAt                                         <  2:481332/205‑1 (MQ=255)
                                                                                                                                                                                                                                        ccGGGCGCCAGTCCTGTTACACGAAAACTTTTGTGGATAGACAGCGCCGCCCCGCGCGTGTTTTCCGCAATATACGCGCCGGAGTTGATCATGATGGGGACCCCCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCa  <  1:530238/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         
AGCACCAGGGTAATAATCAGATAGAACACCGCCACGGCGCTCCAGATCTCAAGGGCGCGGAAGTTACCGGCAATAATTTCTTGCCCCTGACGGGTCAGTTCCGCCACGCCGATCACAATAAACAGCGAGGTGTCTTTAATGCTGATGATCCACTGGTTACCCAGCGGCGGCAGCATACGACGCAGTGCCAGCGGTAAAATGACGTAGCGAATGGTTTCCCAACGTGAAAGACCGAGCGCCAGTCCTGCTTCACGAAAACCTTTGTGGATAGACAGCACCGCACCACGCGTGATTTCCGCAATATACGCGCCGGAGTTGATCATGATGGTGACCACCGCCGCAGTAAATGGGTCGATGCGTAAGTCGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCA  >  NC_000913/846506‑846975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: