Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,027,203 T→G 6.4% T191P (ACA→CCA)  yccT ← UPF0319 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,027,2030TG 6.4% 182.2 / 2.1 65T191P (ACA→CCA) yccTUPF0319 family protein
Reads supporting (aligned to +/- strand):  ref base T (35/24);  new base G (3/2);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTACCGGAATCGGCGTCGCATCGCCATCCAACAATTCAAG  >  NC_000913/1026965‑1027428
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
cacccGCCCAATTTGTTTCCGGGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCtgt                                                                                                                                                                                                                                   <  1:843656/239‑1 (MQ=255)
  ccaGCGGAATATGTTGCCGGGCAGGTAAAGTGCGGGTCGGCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGa                                                                                                                                                                                                                                 <  2:409134/239‑1 (MQ=255)
      cgcAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGCTGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTTGGAGCa                                                                                                                                                                                                                              >  2:898572/1‑238 (MQ=255)
      cgcAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGAATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTGGTTCTGTGAGCa                                                                                                                                                                                                                              >  2:333320/1‑238 (MQ=255)
               tttCCGCGGAGGTAAAGGTGGATTCTTCAGTTCCATAATGTACATCCGTAATTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTg                                                                                                                                                                                                                    <  1:436767/239‑1 (MQ=255)
               tttCCCCGCGGTAAAAGTGGGATTTGCCGGTTCCATAATGTACAACCGTTGTTAACTTTCCTACAAATTACGTTAAGCACTTAGTGGGGCAAGATAAAGACCGAAAACGCCGGCGCACAGGCACAAAAATCTCAGGAAGATGGGTGTTTTTCCTCCCCCGGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACTTGCGACTg                                                                                                                                                                                                                    <  1:557226/239‑1 (MQ=255)
                ttacgcgAAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTACCTTTCCTACAGCTTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGAATTTCGCGTTTTGGGTTACACCAGCTTACACCCAACATTCAACCGTTGTTCTGTGGGCACCTAAGGCTgg                                                                                                                                                                                                                   >  1:767871/4‑238 (MQ=255)
                ttCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCAAAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGAATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGGGACtcc                                                                                                                                                                                                                   >  2:93565/1‑237 (MQ=255)
                 tcggcgcAGGTAATGTGTGAGTCGTCAGTCCCATAATGTACATCCGGATGTAACTTTCCCACAGATTGCTGTAAGCACTTATCGATGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCg                                                                                                                                                                                                                  <  1:773559/236‑1 (MQ=255)
                     cgcAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCCGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCACAACTTCAGCCGTTGTTCTGTGAGCACCTGCAACTGCgggga                                                                                                                                                                                                              >  1:175625/1‑236 (MQ=255)
                     cgcAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTTCGGTgg                                                                                                                                                                                                              >  2:262435/1‑239 (MQ=255)
                     cgcAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGCGACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCCGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAACCCAATACTTCAGCCGTTGTTCTGGGAGCACCTGAGACTACGATgg                                                                                                                                                                                                              >  2:324773/1‑239 (MQ=255)
                         ggTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATCCTTCAGCCGTTGTTCTGTGAGAACCTGAGACTGCgtgggaga                                                                                                                                                                                                           >  2:656572/1‑231 (MQ=255)
                              agtcagattcttcAGTTCTATGATGTACATCCGTAGTTAACGTTCCTTCTGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAACCCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATc                                                                                                                                                                                                     <  1:210929/228‑1 (MQ=255)
                                    aGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGAAAGATAAAGCACGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGCAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGAATTTCGGGTTTGTGGGTCAGCAAGTTTAAACCAATACTTAAGCCGTTGTTATGTAGGCCCCTGCGACTGCGTGGGaaaaagaaccagcgc                                                                                                                                                                                               >  1:572574/1‑226 (MQ=255)
                                       cggcAGTTCCATAATGTACCTCCGTAGTTAACTTTACTACAGATTACTGTAAGCCATTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCTCAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATGCTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGa                                                                                                                                                                                            <  2:704078/236‑1 (MQ=255)
                                         tcATTTCCATAATGTACCTCCGTAGTAAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGaaa                                                                                                                                                                                          <  1:470021/239‑1 (MQ=255)
                                                cATAATGTCCATTCGTTGTTAACTTTCCTACAGATTACTGTAAGCACTTATCACTGCAAGAAAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGa                                                                                                                                                                                   <  1:231956/239‑1 (MQ=255)
                                                     tgtaactCCTTAATTAACTTTCCTACAGATTACTTGAAGCACTTAACGCTGCAAGATAAAGGCCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATGTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAagca                                                                                                                                                                              <  1:285116/233‑1 (MQ=255)
                                                       aacagcagTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAATACCGAAAAAGCCTGCGCACATGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGCATTTCGCGTTCGTGGGTACGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTTACCACCTGTAACTGCGGTGGAAAAGCATCCAACCCGGGAACACCCGCAaacaac                                                                                                                                                                            >  1:883566/8‑237 (MQ=255)
                                                          ctcctttgTTAACTTTCCAACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAAGCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCAGCCAGCTCGGAAACACCCGAAAGCAGCGTa                                                                                                                                                                         <  1:898572/232‑1 (MQ=255)
                                                          aTCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGCAAGAATTTCGCGTTTGTGGGTCGGCCAGTTTAAACCACTCCTTCAGCCGTTGTTCTGTGAGCTCCTGAGACTTCGCGGGAATAGCATCCACACAGGAAAAACCCaaaaaaacgtct                                                                                                                                                                         >  1:60840/1‑231 (MQ=255)
                                                            ccGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAATCCTGCGCACAGGCACAAAAATCTCAGCAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGATTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCTTTGTTCTGTGAGCACCCGAGACTTCGGTGGAATa                                                                                                                                                                                                          >  2:402105/1‑204 (MQ=255)
                                                               tAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTg                                                                                                                                                                                                                    >  2:136133/1‑191 (MQ=255)
                                                               tAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTg                                                                                                                                                                                                                    <  1:136133/191‑1 (MQ=255)
                                                                   tAACTTTCCTAAAGATTCTTGTAAGCCCTTAGTGCTGCAAGATAAAGCCCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTCTCCGCCCACTGCAGTAAAGTATTTCGCGTTTGTGGGTCAGGCAGGTTAACCCAATACTTCAGCCGTTGTTCTGTGAGCCCCAGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCAt                                                                                                                                                                  <  1:749077/237‑1 (MQ=255)
                                                                         tCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCACTACTTCACCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGCATAGAATCCAGCTCGGAACCACCCGAAACCAGCGTACTGTCATCttcac                                                                                                                                                            >  2:344371/1‑233 (MQ=255)
                                                                                       tgaaGCACTTATCGCTGAAAGATAAAGACCGAAAAAGCCTGCGCAAAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATTCTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTg                                                                                                                                            <  1:333320/237‑1 (MQ=255)
                                                                                        tAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCACCTCGGAAACACCCGAAAGCCGCGTAATGCCATATGCAACAACCGCGGCAAAATGc                                                                                                                                           >  1:74665/1‑239 (MQ=255)
                                                                                         aaGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTGAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGGGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCAGCATCGTGGCAAATTGCg                                                                                                                                          <  1:572550/239‑1 (MQ=255)
                                                                                          aGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGAc                                                                                                                                                                                                                      <  1:836018/162‑1 (MQ=255)
                                                                                          aGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGAc                                                                                                                                                                                                                      >  2:836018/1‑162 (MQ=255)
                                                                                          aGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCgg                                                                                                                                         >  2:779871/1‑239 (MQ=255)
                                                                                          aGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCgg                                                                                                                                         >  2:680260/1‑239 (MQ=255)
                                                                                               ttATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCATCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCCACTCGGAAACACCCGAAAGCAGCGTACTGTAATCTGCCTTCACCGTGGCAAATGGCGGGAgga                                                                                                                                    >  1:384863/1‑237 (MQ=255)
                                                                                                tATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTgaga                                                                                                                                                                                                                       >  1:27138/1‑155 (MQ=255)
                                                                                                tATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTgaga                                                                                                                                                                                                                       <  2:27138/155‑1 (MQ=255)
                                                                                                tATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGa                                                                                                                                   >  2:716886/1‑239 (MQ=255)
                                                                                                              aaaGACCGAAAAAGCCTGCGCACAGGCACAAAATTCTCAGGGAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGGGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCgg                                                                                                                     <  1:554319/239‑1 (MQ=255)
                                                                                                                     gAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAACGCCGGTTACTGGCATCTGCCATCATCGAGGCAAATTGCGAGAGGGAAACGGGttgccggggttgggt                                                                                                              >  1:301266/1‑226 (MQ=255)
                                                                                                                     gAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGGGAGCACCTGAGAc                                                                                                                                                                                                                      <  1:726777/135‑1 (MQ=255)
                                                                                                                     gAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGGGAGCACCTGAGAc                                                                                                                                                                                                                      >  2:726777/1‑135 (MQ=255)
                                                                                                                      aaaaaGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGCAAGCAGCGTACTGTCATCTGCCACCATCGTGGCAAATTGCGGGAGTGAAGCGCGGTTCCGGGAGTTTTta                                                                                                             >  1:634110/1‑239 (MQ=255)
                                                                                                                      aaaaaGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGGTTTGTta                                                                                                             >  2:845862/1‑239 (MQ=255)
                                                                                                                                   acaGGCAAAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCt                                                                                                <  2:287077/239‑1 (MQ=255)
                                                                                                                                          caAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGGGGCAACTTCCGGTAGGGAAGCGCGTTTCGCGGATTTGTTAAAGCTTTCAAcccggacgcc                                                                                         >  1:114437/1‑231 (MQ=255)
                                                                                                                                                 ctcAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGCCTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCACCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTCCCCGGATTTGTTATACGTTTAAACCCCGCCCTCacacaca                                                                                  >  1:18617/1‑233 (MQ=255)
                                                                                                                                                            ggTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAACCAGTCGTTTTTg                                                                        >  2:632404/1‑238 (MQ=255)
                                                                                                                                                               tttttttCCGCCCACTGAAGGAAAGTATTTCGTGTTTGGGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCAACTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGt                                                                    <  1:171340/237‑1 (MQ=255)
                                                                                                                                                                    ttCCCCCCCCTTGAGGAAAGTATTTCGGGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGGTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAgg                                                               <  1:845862/239‑1 (MQ=255)
                                                                                                                                                                              tGCAGGAAAGTATTGCGCGTTTGGGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCgag                                                     <  2:302302/239‑1 (MQ=255)
                                                                                                                                                                                     aattttgtgtggtgtgtgGGACACCCACTTTAAACCAAAACGTCCGGCGTTGTGCCGCGAGACCCCGAGAAGGCGTGGGAATAGCCCCCAGCTCGGAAACACCCGAAAGCAGCGTACGGTCATCGGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGATTTTGCCTTTGAGGTAATGGCGAGAATATc                                               <  1:879000/224‑1 (MQ=255)
                                                                                                                                                                                        tATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGCAACACCCGAAACCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGCATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGCGTTAATGGCGGGACTATccc                                             >  2:605722/1‑236 (MQ=255)
                                                                                                                                                                                        tATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGATTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGCAATGGCGAGAATATCCa                                             >  2:520387/1‑237 (MQ=255)
                                                                                                                                                                                        tATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGAGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGAGGATTTGTTATAGCGTTCAACATCGACCTTATAATCATCCGTTTTTGCCGTTGAGGTATTGGCGGGAATAACCa                                             >  2:59800/1‑237 (MQ=255)
                                                                                                                                                                                          tttCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGGGGGAAAAACAAGCtt                                         >  1:846474/1‑239 (MQ=255)
                                                                                                                                                                                          tttCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTAATCTGCCTTCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATACCGTTCACCCCCGACCTACTAATCAATCGTTTTTGCCGTTGCGGTAATGTCACGAACATCCACCtt                                         >  2:837828/1‑239 (MQ=255)
                                                                                                                                                                                             cgcgTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAag                                                                                                                                                                                >  2:652048/1‑101 (MQ=255)
                                                                                                                                                                                             cgcgTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAag                                                                                                                                                                                <  1:652048/101‑1 (MQ=255)
                                                                                                                                                                                               cgTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTATTGGCGAGAATATCCAGCTTTACCg                                    >  2:326237/1‑239 (MQ=255)
                                                                                                                                                                                                         tCAGCCAGTTTAAACCAATACTGCAGCCGTTGTTCTGGGGGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTACCGGAATCGGCg                           <  2:18617/238‑1 (MQ=255)
                                                                                                                                                                                                             gCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTAACGGAATCGGCGTc                         >  2:626346/1‑237 (MQ=255)
                                                                                                                                                                                                                   ttAAACCAATGCTTCAGCCGTTGTTCGGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACATCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTACCGGAATCGGCGTCGCATCg                   <  2:767871/237‑1 (MQ=255)
                                                                                                                                                                                                                         cAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTACCGGAATCGGCGTCGCATCGCCATCcaa           <  2:634110/239‑1 (MQ=255)
                                                                                                                                                                                                                            tACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGCGAATATCCAGCTTTACCGGAATCggagcggacacgccacccaaaaa        >  1:451704/1‑218 (MQ=255)
                                                                                                                                                                                                                                   gCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGTCGAGAATATCCAGCTTTACCGGAATCGGAGTCGCATCGCAATCCAACAATTCaaa  >  2:93871/1‑237 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                 
CACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGCGGTAGTGAAGCGCGTTTCGCGGATTTGTTATAGCGTTCAACCTCGACCTCATAATCAATCGTTTTTGCCGTTGAGGTAATGGCGAGAATATCCAGCTTTACCGGAATCGGCGTCGCATCGCCATCCAACAATTCAAG  >  NC_000913/1026965‑1027428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: