Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 71,877 A→G 23.0% E176G (GAG→GGG)  yabI → DedA family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091371,8770AG23.0% 101.6 / 2.7 55E176G (GAG→GGG) yabIDedA family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (10/29);  new base G (12/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 2.42e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTT  >  NC_000913/71650‑72113
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGTCTGCCGGCTGTGGCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGCTATTCCTGCCGGACTGCAGAGCGGGGGGATTAAATGGt                                                                                                                                                                                                                                     >  1:260966/1‑239 (MQ=255)
aaCTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTTTTACGCCGAATATTATCGGCGGCCTGCTGGGGCCGCCCTTTTACTTCCTCCCAGGGATTCTGGCGGGGCCGGCGCTCGGTATTCCTGCCGGAAGGGAGAGCGGGGAGGTTAAAAggg                                                                                                                                                                                                                                     >  1:204918/1‑238 (MQ=255)
  cTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGGGGCCGCCGGTTTACTTCCTGCCAGGGTTTCTGGGGGGCGACGGGGTCCGATTTCCTTCCGGACAGCCGAGCGGGGGGTTTAAAGGGTtg                                                                                                                                                                                                                                   >  1:105587/1‑239 (MQ=255)
     aaCATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCGGCCAGGGATTCTGGAGGGCGCGGGGTTCGGCATTCCTCCCGGAATGCAGAGCGGGGCGTTTAAATGGTtgct                                                                                                                                                                                                                                 >  2:297963/1‑238 (MQ=255)
           cGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTCAATTTTTTACCCCGAATCTTATCGGCTGCCTTCCTTGGCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGCTCGCATTTTCTGCCGGAATGTAGGGCGGGGGGTTTAAAAGGTTGCTGCGGGCa                                                                                                                                                                                                                          >  1:436346/1‑239 (MQ=255)
            tttgcataaaccagggtgggtccccATTCGGGGCGGTCTGTTTGTTGGCCCGACGCGTCCGTTGGGGCCAAGGGTGGCGGGAAGGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGc                                                                                                                                                                                                                           <  2:443190/214‑1 (MQ=255)
               gCATCAACCCAGCATGGTCACCATTCTGGTGGGTCGTTTTGTTGGCCCGACGTGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAg                                                                                                                                                                                                                      <  2:450098/239‑1 (MQ=255)
               gCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGAGCGGTATTCCTTCCGGAATGCAGAGCGGTGAGGTTAAATGGTTGCTGCTGGGAAAAg                                                                                                                                                                                                                      >  2:830574/1‑239 (MQ=255)
                          gCATGTTTACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATTCTGGATCTGCCGGTGGCTAAATTTATTATGCCGATTATTATCGGCTGCCTGCTGTGGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGGCGGGGGGTGGACATTACTGCCGGAAGTCGGACGGGGGGGTTTAAATGGTtgctgtggggaaacgcgggggttttt                                                                                                                                                                                                           >  1:198233/1‑218 (MQ=255)
                          gCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTGCCGGAATGCAGAGCGGGGGGGTTTAATGGTTTCTGCCGGCCAAAGCGGGGgttttt                                                                                                                                                                                                          >  2:537419/1‑238 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTCAATTTATTACGCCGAATATTATCGGCTGCCTGCTGGGGCCGCCGTTTTACTTCCTGCCCAGGATTCTGGCGGGCGCGGCGGTCGGAATTCCCTGCGGAATGACGAGGGGGGAGTTTGAATGGGTTGCGGCGGGAAAcccgggggttttgg                                                                                                                                                                                                           >  1:779347/1‑226 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGGATATTATCGGCTGCCTGCTGTTGCCGCCGGTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGCTCGCTATTCCTCCCGGGATGCAGCGCGGGGGGGTTAAATGGGTGCGGCTGGGAACCGCGGGGTGTTtgt                                                                                                                                                                                                         >  1:84490/1‑239 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGGTATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGGTTTtgt                                                                                                                                                                                                         >  2:897135/1‑239 (MQ=255)
                           cATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGAGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGCTATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTtgg                                                                                                                                                                                                         >  2:199096/1‑238 (MQ=255)
                                tCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTTTGGCGGGCGCGGCGGTGGATATTCCTTCCGGAATGCGGAGCGGGGGGTTTTAATGTGTGCTGCTTGGACCAGCGGGGTTTTTTTGGGtg                                                                                                                                                                                                    >  1:607086/1‑238 (MQ=255)
                                    tatgcgggtgggtcGTTTTGTTGGCCCGACGCGTCCTCTGGTGCCAATGGTGGCGGGAATGCGGGATCTGCCGGTGGGTAAATTTATTCCGCCAAATATTATCGGGTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTtggtg                                                                                                                                                                                                <  2:209489/233‑1 (MQ=255)
                                         tGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGGATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTTCCGGGATGCGAAGCGGTGGGGTTTAATGGGTTCTGCTGGCCACAGCGGGGGTTTTGTGGGtgtgggggggg                                                                                                                                                                                           >  1:426289/1‑229 (MQ=255)
                                         tGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGGGGGGTTAAATGGTTGCTGCTGGCAACAGCGGGGTTTTTGGGGGTTGgtgggtgg                                                                                                                                                                                           >  2:399440/1‑239 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGCATGCTGGATCTGCCGGTGGCTTACTTTATTAAGCCGAATATTATCGGCTGCCTGCCGTGGGCGCCGTTTTACTTTCTGCCCGGGGTTCTGGCGGGCGCGGCGGGCGGTGTTCCTGCCGGAATCCGGGAGGGGGGGATTTAACTGGTTGTGCTGGCAAACACGGGGGTTTTGTGGGGtgggggggggtg                                                                                                                                                                                         >  1:789454/1‑233 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGGGGGGGGGTCCGTATTTCTGCCGGGATGCAGAGTGGGGGCGTTAAATGGTTTTTGTTGGGAAAACCGGGGTTTTTtgggggtggggggggggg                                                                                                                                                                                         >  1:882508/1‑233 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGGTCGATATTCCTGCCGGAATGCAGAGCGGTGGGTTTAAATGGTTGCTGCTGGCACCACCGGTGTTTTTGTGGGGTGGtggcgg                                                                                                                                                                                            >  2:639977/1‑234 (MQ=255)
                                           gtcggtcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGGGAGTTTAAATGGTTGCGGCTGGCAACAGCGGTGGTTTTGTGGGTTGGtggcgg                                                                                                                                                                                            >  2:284905/1‑234 (MQ=255)
                                                tcgTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAAAATTATCGGCGGCCTGCTGTGGCCGCCGCTTTACTTCCTGCCCGGGATTCTGGCGGGCGCGGCGCTCGATATTCCTGCCGGGATGCGGAGGGGTGAGGTTATAATGGGGTGGCTGGGAACAGCGGGGGTTTTTGGGGGTgggggtggggatg                                                                                                                                                                                        >  1:796909/1‑226 (MQ=255)
                                                 tgTTTTGTTGGCCCGGCGGTTCCGCTGGGGCCAAGGGTGGGGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTACCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  2:848409/237‑1 (MQ=255)
                                                 ctttttGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCGGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  2:84490/236‑1 (MQ=255)
                                                 cgTTTTGTTGGCCCGACGGGTCCGCTGGTGCCAATGGGGGCGGGAATGGTGGATGTGCCGGTGGATAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCt                                                                                                                                                                                    <  2:706282/238‑1 (MQ=255)
                                                                aCGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAAAGGTTGCTGCTGGCAACAGCGGGGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCggggtttgtgccg                                                                                                                                                                    >  2:161199/1‑228 (MQ=255)
                                                                            gTGCCAATGGTGGGGGGAATGCTGGATTGGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGa                                                                                                                                                         <  2:609216/238‑1 (MQ=255)
                                                                             gtccatggggggggggattgtgggATCGGCCGGTGCCTAAATTTTTTCCGCGGATTATTACCGGTTGCCTGCGGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACt                                                                                                                                                       <  1:537419/217‑1 (MQ=255)
                                                                              gCCAATGGTGGGGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTCCGCCGAATATTAGCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACt                                                                                                                                                       <  2:204918/238‑1 (MQ=255)
                                                                                      tggGGGAAATGGTGGTTCTGCCGGGTGTAAATTTTTTTCCCGCGAATTTTGTGGGCGGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGttt                                                                                                                                               <  1:161199/238‑1 (MQ=255)
                                                                                           ggAATGCTGGATCTGCCGGGGGCTAAATTTATTACGCCGAATTTTAGCGGCTGCCTGCGGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTc                                                                                                                                          <  2:426289/238‑1 (MQ=255)
                                                                                                         gCCGGTGGCTAAATTTATTACGCCGAATATTACCGGCTGCCGGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCcgcg                                                                                                                           <  2:423867/239‑1 (MQ=255)
                                                                                                               ggCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGt                                                                                                                      <  2:723286/238‑1 (MQ=255)
                                                                                                                    aaatttttgtcccggAATATTACCGGCTGCCTGCTGGGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTttgtt                                                                                                                 <  2:243121/225‑1 (MQ=255)
                                                                                                                           taCGCCGATTATTAGCGGCTGCCTGGTGTGGCCGACGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGa                                                                                                         <  2:207293/239‑1 (MQ=255)
                                                                                                                                   aaTATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCACCAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATTGGGCAGCGGTGAAGCGAACGGCCGTTTCGGTCATTTTTTTTTCCGCGGCCggtggtgttgggcgcagtgcgc                                                                                                  >  1:718278/1‑218 (MQ=255)
                                                                                                                                                 cctgcTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTTTGGGGCAGCGGGGAAGAGACCGACCGCTTTGGTCCTTTTTTTTTCCCCGGGCGGTTTTTTTGGGTGAGCCCCGTTGtttttctcctcc                                                                                    >  2:7721/1‑231 (MQ=255)
                                                                                                                                                      tgtgGCCGCCTTTTTCCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtg                                                                              <  2:819452/239‑1 (MQ=255)
                                                                                                                                                         ggCCGCCGTTTTTCTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCgtggt                                                                            <  2:260966/238‑1 (MQ=255)
                                                                                                                                                           gccccttttttcttctcgaccgggTTTCGGGCGGGCGCGGCGATCGATATTCCGGCCGGAATGCAGTGTGGTGAGTTTAAATGGCTGCTGCTGGCAACAGGGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTTACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGtggtgg                                                                          <  1:814666/217‑1 (MQ=255)
                                                                                                                                                             gccgTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGgcgc                                                                       <  2:789454/239‑1 (MQ=255)
                                                                                                                                                                                 ggtttCTGGCGGGGGGGGGGATCGATTTTCCTGCCGGAAGTCAGAGGGTTGGGTTTAAATGGTTGGTTCTGGCAACAGCGGTGTTTTGGTGGGTTGGTGGCTGGCTTGGCTGGGGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCa                                                    <  1:656616/235‑1 (MQ=255)
                                                                                                                                                                                           gggcgCGGGGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                          <  2:607086/238‑1 (MQ=255)
                                                                                                                                                                                            ggggggggggtttgttatTCCTGCCGGAATGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTGTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATg                                          <  1:7721/223‑1 (MQ=255)
                                                                                                                                                                                             ggccgggcgTTGGATTTTCCGGCCGGAATGCAGGGGGGTTTGTTTAAATGGTTGCTGGTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGc                                         <  1:399440/233‑1 (MQ=255)
                                                                                                                                                                                                     gatcgatATTCCTTCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTTGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtat                                 <  2:500501/238‑1 (MQ=255)
                                                                                                                                                                                                             ttCCGGCCGGAATGCAGGGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtt                        <  2:436346/239‑1 (MQ=255)
                                                                                                                                                                                                                            gaggggggTGGGTTTAAATGGTTGCTTGGGGGAACAGGGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATCCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  1:284905/233‑1 (MQ=255)
                                                                                                                                                                                                                            gagagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  2:104492/238‑1 (MQ=255)
                                                                                                                                                                                                                             agggcgggtaGTTTAAAGGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  2:737373/229‑1 (MQ=255)
                                                                                                                                                                                                                             agagCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGt          <  2:882508/238‑1 (MQ=255)
                                                                                                                                                                                                                               atcGGTGAGTTTAAAGGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTg        <  2:508028/236‑1 (MQ=255)
                                                                                                                                                                                                                                    tGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGtt   <  2:779347/238‑1 (MQ=255)
                                                                                                                                                                                                                                    tGAGTTGAAATGGTTGCTGGTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGtt   <  2:357728/238‑1 (MQ=255)
                                                                                                                                                                                                                                     gAGTTTAAATTGTTGCTGCTGGCAACAGCTGTGTTTTTGTGGGTTGGTCGCTGGCTGTGCGTGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGGTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGttt  <  2:404765/238‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                
AACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTT  >  NC_000913/71650‑72113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: