Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,240,710 A→G 10.1% H454H (CAT→CAC)  cvrA ← putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,240,7100AG10.1% 195.3 / 2.2 73H454H (CAT→CAC) cvrAputative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (30/32);  new base G (3/5);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAA  >  NC_000913/1240480‑1240940
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
tcccccAGCGTTTGCTGCTTAACCCGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATAGTTGGCGCGGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCaa                                                                                                                                                                                                                                 <  2:34371/236‑1 (MQ=255)
tCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCaa                                                                                                                                                                                                                                 <  2:602600/239‑1 (MQ=255)
               tgctTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGaaa                                                                                                                                                                                                                  <  2:190378/239‑1 (MQ=255)
                  ttataagagacccgtgtcccGTTTGCCCGGGCATATATCAGCGAAACATCAGGAATTTTGGGGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAg                                                                                                                                                                                                               <  1:118407/222‑1 (MQ=255)
                    aTCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACCTCGCCTTCGCGCAGTCGGGGGCTGCCGGTAGGATGAAGCAACTGGTTATCACCAAACAGt                                                                                                                                                                                                              >  2:700474/1‑238 (MQ=255)
                            agtcgcGCCCGTCTTCCAGGCCATATACCAGCGCAACATCAGCATATTTGGCGGTGGCGTCGAGAATGAAGTCACCAATGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATa                                                                                                                                                                                                     <  2:102898/234‑1 (MQ=255)
                               ctcGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAAAAGTGCCGCAATCcgc                                                                                                                                                                                                  >  2:70816/1‑239 (MQ=255)
                                tcGCCCGTCTTCCAGGCCATATAACAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg                                                                                                                                                                                                 <  2:748244/239‑1 (MQ=255)
                                 tgCCCGTCTTCCAGGACATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                                                                                                                                                <  2:673993/238‑1 (MQ=255)
                                 ccccccTCTTCCCGGCCATTTATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCCCCAAAGAAGCGTTGAACCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                                                                                                                                                <  1:886168/237‑1 (MQ=255)
                                 cGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                                                                                                                                                <  2:489954/239‑1 (MQ=255)
                                     cGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcccgcgcct                                                                                                                                                                                            >  2:793234/1‑234 (MQ=255)
                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGATCGCCGGAAGATCGCGTTCCCGACCAATTACACCCAA‑CACATCCCCTTCTGCAAGACGGGTGCTGCCGGTAAGGTGACGCAACAGGTTTGCACAAAACATAGCCGCAAAAACCGTCCCttt                                                                                                                                                                                          >  1:673993/1‑239 (MQ=255)
                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACCCGCCTCttt                                                                                                                                                                                          >  2:701147/1‑239 (MQ=255)
                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGAAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACACGTCTCttt                                                                                                                                                                                          >  2:308027/1‑239 (MQ=255)
                                       tCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCCTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGGAGGATGCAGAAACTGGTTATCACGAAAAAGGACCGCAAAACGCGGCCCttt                                                                                                                                                                                          >  1:316776/1‑239 (MQ=255)
                                          tCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGGTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑Tgg                                                                                                                                                                                      <  2:299875/239‑1 (MQ=255)
                                          tCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGCCCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑Tgg                                                                                                                                                                                      <  2:408215/239‑1 (MQ=255)
                                          tCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGGTATCACGAAAAAGTGCCGCAATa                                                                                                                                                                                                     >  2:118407/1‑225 (MQ=255)
                                           ccAGGCCATATAACAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACGCACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGc                                                                                                                                                                                     <  2:93037/239‑1 (MQ=255)
                                            cAGGCCATATTACAGCGCAACATCAGCATATTTGGGGGTGGCTTCGAGAATGAAGTCCCCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCa                                                                                                                                                                                    <  1:37660/239‑1 (MQ=255)
                                                    atatCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGGTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCaaa                                                                                                                                                                            >  2:172120/1‑239 (MQ=255)
                                                      atCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGCAGGATGAAGCAACGGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAAt                                                                                                                                                                           >  2:662938/1‑238 (MQ=255)
                                                         aGCGCAACCACAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATcgcg                                                                                                                                                                       <  2:373099/239‑1 (MQ=255)
                                                         aGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCACTCGGGGGCTGCCGGTAGGATGAAACAAATGGGTATCACGAAAAAGGGCCGCAATACACGCACCTTT‑AGGCATATGAAAAAcgcc                                                                                                                                                                       >  1:389218/1‑238 (MQ=255)
                                                         aGCGCAACATCAACATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCCGTCGGGGGCGGCCGGTGGGGTGAAGCAACCGGTTTTAACGACACAGTGCCGCAAAACGCCGCTCATT‑TTGCATATGAAAAgcgc                                                                                                                                                                        >  1:391214/1‑238 (MQ=255)
                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGGCCAATTACACACAA‑AACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGCTGAAGCAACTGGTTTTCAATAAACATGACCGTAAAACGCGTACCTTT‑TGGAATATGGaaaaacaaca                                                                                                                                                                     >  1:382026/1‑232 (MQ=255)
                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCCAATCgcgcc                                                                                                                                                                     >  2:160915/1‑238 (MQ=255)
                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCCCTTT‑TGGCATATGCAAATCGCGCa                                                                                                                                                                     >  2:258685/1‑239 (MQ=255)
                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCCGCCGGTAGGATGAAACAACTGGTTAATCCGGAACAATGCCGCAATACGCGCCCCTTT‑TGGCCTATGCAAAAcgccag                                                                                                                                                                     >  1:850964/1‑236 (MQ=255)
                                                           cgcAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGCCGGGGGCTGCCGGTAGGCCGAAGCCACTGGTTATCACGAAACCATGCCGCAATACGCGCCTCTTT‑TGGCATATGCAAATcaccca                                                                                                                                                                     >  2:482647/1‑234 (MQ=255)
                                                             cAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCCATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCCCGAAACAGTGCCCGAAAACACGGCTCTTT‑TGGCATATGAAAATccagaag                                                                                                                                                                    >  1:772660/1‑232 (MQ=255)
                                                             cAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATAACGAAACAATTCCGCAATACTCGTCTCTTT‑TGGCATATGCAAAtactgcag                                                                                                                                                                    >  2:344575/1‑231 (MQ=255)
                                                               aacccAGCATATTTGGGGCTGGCTTCGGGAATGAAGTCACCAAAGAAGCGTTTAACCAGGGGGACCGGGGGCCAATGGCTGAACAGGTTTCCGAGGCCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGc                                                                                                                                                                 <  1:136184/235‑1 (MQ=255)
                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGGTGGAGCAACTGGTTATCCCGAAACAATGCCGCAAAACGCGGCTCATT‑TGGAAAATGCAAATAGCGCaaggcc                                                                                                                                                                >  1:295340/1‑234 (MQ=255)
                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTa                                                                                                                                                                                                                          >  2:585115/1‑182 (MQ=255)
                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTa                                                                                                                                                                                                                          <  1:585115/182‑1 (MQ=255)
                                                                cATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGAACAATTACACACAA‑CACATCGCCTTACCGCATCCGGGTGCTGCCGGTAGGCAGCAGCAACTGGTTATAACGAAAAAGGACCGCAATACACGCCTCTTT‑TGGCATATGCAAATCGCACAGGGcc                                                                                                                                                                >  1:566832/1‑239 (MQ=255)
                                                                 ctCAGCATATTTGGCGGTGGCCTAGAGAATGAAGCCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCcg                                                                                                                                                               <  2:707112/238‑1 (MQ=255)
                                                                 aTCAGGTTATTTGGGGTCGGCTTGGGAAATTAAGGCCACAAAGAAGGTTGGAACCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCCCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCcg                                                                                                                                                               <  1:129977/239‑1 (MQ=255)
                                                                 aTCAGCATATTTGGGGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCcg                                                                                                                                                               <  2:379463/239‑1 (MQ=255)
                                                                     gCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGGGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGGGCCGCAATACGCGTCTCTTT‑TGGCATATGAAAATCGCGCAGTGCCgcgc                                                                                                                                                            >  2:819533/1‑238 (MQ=255)
                                                                       atatTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGGGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCa                                                                                                                                                         <  2:528154/239‑1 (MQ=255)
                                                                         ttttGGGCCTGGCTGCGAGAATGAAGGCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACg                                                                                                                                                       <  1:701147/238‑1 (MQ=255)
                                                                            tGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAACGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCAc                                                                                                                                                    <  2:391214/239‑1 (MQ=255)
                                                                                gcTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGGGCCGCGCCCACGCACCAtt                                                                                                                                                >  2:741532/1‑239 (MQ=255)
                                                                                        cGAGAATGAAGTCACCAAAGAAGCTTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCa                                                                                                                                        <  2:389218/239‑1 (MQ=255)
                                                                                                               cGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGGTAAACCAACTGCt                                                                                                                  >  2:723857/1‑238 (MQ=255)
                                                                                                               cGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCCCAATACGCGGCTCTTT‑TGGCATATTCAAATCGCGCAGTGCCGCCCCCACCCCCCCTTTATCGGCCCCCAATTGTAaaaaaaaccacc                                                                                                                  >  1:482883/1‑234 (MQ=255)
                                                                                                                         gcgcGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGGGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAATGCCGCGCCCAGGCACCATTTATCGGCCCTCCATTGATTAACAAACTGCCCCCCCGGAttt                                                                                                       >  2:625045/1‑239 (MQ=255)
                                                                                                                           gccctcggggtcgccTGGCGTAACAGTTGTCCGGGCGCCAGAAGATCGGTGTCCCGAGCAATTACACAAAA‑ACCACCCCCTGCGCGGCGTCGGGTGCTGCCGGTAGGGTGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTGTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTc                                                                                                     <  1:308027/225‑1 (MQ=255)
                                                                                                                             gccccgcggcgCATGGTTGAACGGTTTCCCGGGGCCCGGAAGTACGCGTTCCCGACCAATTACACACAA‑CACATCTCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTAACACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCg                                                                                                   <  1:798616/234‑1 (MQ=255)
                                                                                                                             gACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGAGCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCg                                                                                                   <  2:316776/239‑1 (MQ=255)
                                                                                                                              aCCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCgg                                                                                                  >  2:242617/1‑239 (MQ=255)
                                                                                                                                   cggccACTGGCTGAACAGTTTACCGGGCCGCGGAAGATCGCGGTCCCAGCCAATTACCCCCAA‑CACATCGGCTTCGGGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGa                                                                                              <  2:343092/238‑1 (MQ=255)
                                                                                                                                               tcaCAGTTTACCGAGCGCCGGAAGATCGCTTTCCCGACCAATTACACACAA‑CCCATCGCCTTCGCGCAGGCGGGTGCTGCCGGTAGGGTGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCa                                                                                  <  2:542908/236‑1 (MQ=255)
                                                                                                                                               gaaccttttACCGGGCTCGGGAGGATCGGGTTCCGGACCAATTACCCCCAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCa                                                                                  <  1:172120/232‑1 (MQ=255)
                                                                                                                                                    tgttACCGCGCGCCCGCAGACCGGGTCCCCGCCCAAATACACACAATTCCATC‑CCTTGGCGCAGTCGGGGGCTGCAGGTAGGGTGAAGCAACTGGTTATCCCGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGt                                                                            <  1:625045/237‑1 (MQ=255)
                                                                                                                                                       tACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGCTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCCCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCACTTGGTAAACAAACTCCCCCCACGGATTTTCCGGAGGAATATCCAGGCCAAcccaaaac                                                                         >  1:540659/1‑232 (MQ=255)
                                                                                                                                                               gcCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACCCGTGACACCggcc                                                                  >  2:243962/1‑236 (MQ=255)
                                                                                                                                                                     aggttctgtcccccACAAATTACACACAA‑CTCATCCCCCTAGGGCAGTCTGGTTCTTGCGGGAGGATGAAGCAACTGGTTATCGCGAAACAGTGCCGCAATACGCGTATCTTT‑TGGCATAATCAGATCGCGCATGGCCGCTCCCACGCACCATTTATCGGCACTCAAGTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCa                                                            <  1:290040/228‑1 (MQ=255)
                                                                                                                                                                     aGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGGCGGGTGCTGCCGGTAGGATGAAGCAACTGGGTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCa                                                            <  2:382026/238‑1 (MQ=255)
                                                                                                                                                                         cgcgTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCCCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCCCCCACGGCATTTCCGAAGGAATTTCCAGGCCAACAGGGGAACCCGAACGTCCCCCGGGc                                                       >  1:763968/1‑239 (MQ=255)
                                                                                                                                                                           ggTTCCCCACCAATTCCCCACAA‑CCCGTCCCCTTGGCGCTGTTGGTGGGTTCCGGTAGGGTGAAGCAACTGGTTAACCCGAAACAGTGCCGCAATACGCGTCTTTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCgg                                                     <  1:662938/238‑1 (MQ=255)
                                                                                                                                                                              tCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCCCCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACCCTGGCCACCGGACGTCCCACTGGCggga                                                   >  2:422610/1‑236 (MQ=255)
                                                                                                                                                                                                aa‑cacaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACTTCCCACTGGCGGAACCACCACTTTGGCcttttt                                >  1:276197/1‑239 (MQ=255)
                                                                                                                                                                                                   cacaTCGCCTTCGCGCAGGCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATAGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCCGGCCACCGCGTGACACCGGCGCGCCCCCTGGCGGGACCACACCTTTGGCTTTTTTa                               >  1:411898/1‑238 (MQ=255)
                                                                                                                                                                                                        cGCCTTCCCGCAGTGGGGTGCTGCCGGTAGGAGGAAGCAACTGGTTATCACGAAACAGTGCCGCAATCCGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAgccgcc                         <  1:741532/239‑1 (MQ=255)
                                                                                                                                                                                                             ttcgggaggcGGGGGCTGGCGGTAGGGAGAAGCAAATGGTGAAGCAGAAAAAGGGCCGCAATACGGGT‑TCTTTGTGGCAGAGGCAAAGCGCGCAGGTCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACga                    <  1:160915/230‑1 (MQ=255)
                                                                                                                                                                                                               gcgcAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACgaga                  <  2:566832/239‑1 (MQ=255)
                                                                                                                                                                                                                    gTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGCAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCACTTGATAAACAAACTGCTCCCACGGCTTTTCCGGATGAATATCCAGGCCAACGCGTGATACACGACGTCCCCCTGGCAGAACCACCCCTTTTGCCTTTTTAGccccccccaaacatgaa             >  1:8469/1‑228 (MQ=255)
                                                                                                                                                                                                                             gtccGGTAGGATGAAGCAACTGGTTACCACGAAAACGGGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAAGCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGc    <  2:864769/237‑1 (MQ=255)
                                                                                                                                                                                                                               ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTACACGCCCACGAGAGTGATGTTCCCTGCaa  >  2:188381/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                       
TCACCCAGCGTTTGCTGCTTATCACGATATTCTCGCCCGTCTTCCAGGCCATATATCAGCGCAACATCAGCATATTTGGCGCTGGCTTCGAGAATGAAGTCACCAAAGAAGCGTTGATCCAGCGCGACCGGCGGCGACTGGCTGAACAGTTTACCGAGCGCCGGAAGATCGCGTTCCCGACCAATTACACACAA‑CACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTT‑TGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGATAAACAAACTGCTCCCACGGATTTTCCGGATGAATATCCAGGCCAACGCGTGACACCGGACGTCCCACTGGCGGAACCACCACTTTGGCTTTTTTAGCCGCCCACGAGAGTGATGTTCCCTGCAA  >  NC_000913/1240480‑1240940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑