| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,242,879 | T→G | 17.5% | T68P (ACC→CCC) | ldcA ← | murein tetrapeptide carboxypeptidase, LD‑carboxypeptidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,242,879 | 0 | T | G | 17.5% | 139.5 / 2.8 | 66 | T68P (ACC→CCC) | ldcA | murein tetrapeptide carboxypeptidase, LD‑carboxypeptidase A |
| Reads supporting (aligned to +/- strand): ref base T (21/29); new base G (11/4); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.24e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTAT > NC_000913/1242643‑1243115 | gtattGGTGCTCGGTTAGGGGGTTCTGGTCATCCGCCCCAAAGTTTCCTCCAAGCATGGGCCCGCTAAAGGTGGTGACATTGCCAGGCGCCATAAGACCGCACTGAAGGGCGGTAAACTCGCTATGTCCGCAAATGAGAAACGGGTCATGTTGTTGGGGGGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGgtgt < 1:16126/235‑1 (MQ=255) aaTGGTGCTCGGTAAAGGCGTTCAGTTCAACCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTAGGCCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGgtgt < 2:776809/237‑1 (MQ=255) gTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCCCCAGTGCCTGCCAGGCAATATCTGCCA‑TGAAACACCT‑GGCACCGTAACCGCCGCGTACAGCCGGCCCGATGGGTGTggggg > 2:45372/1‑239 (MQ=255) ttcagttcaTCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGGCATGTTGTTGGCGAGGCACCAGTGCCTGCCAGTCAATATCTGCCA‑GGAAACGACC‑GGCACCGTAACCGCCGCGGACCACCAGCACGGCGGGGTTggggggcgtaaggtggggaag > 1:853228/1‑223 (MQ=255) cagttcaTCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGGCACCAGTGCCTGCCCGTCAATATCTGCCA‑GTAAACGACT‑GGCCCCCGAACCGCCGCGTACAGCCAGCACAATGGTGGTGGGGGGAGTAAGGCTGGCAAggg > 1:653571/1‑239 (MQ=255) cagttcaTCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATAACTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACCGCCCGCACCAGCGGGTTAGGGGTAGTAAATCTGGCAAggg > 2:440552/1‑239 (MQ=255) cccGCGCCAAAGTTTGCTCCAAGCATGGGGCCGCTAAAGGGGATGAACTGGCCATGCGCCAGAAGACCGCACTGAAGGGCGGTAAAATCGTTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTgag < 2:43012/238‑1 (MQ=255) ccAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTTAGTCTGGCACGGGAATTGACACCtt > 2:327081/1‑238 (MQ=255) cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑TGCACCGTAACCGCCCCGTCCAGGCAGGCCGATGGGTGTGGGGGTAGGCAGTCCGGGCAGGGAAATAGGAACTTACCAAACGC‑CCGTTTCCGGACCCCCaca > 1:67396/1‑237 (MQ=255) cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGACTGGCAAAGGGATTAGGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCaaa > 2:497061/1‑239 (MQ=255) caaagggaagtgcATTGCCGGGCCCCAGAAGCCCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCaaaa < 2:130636/227‑1 (MQ=255) tgattccATTGCCAAGCGCCAAGAGGCCGGCCTGAATGGCGGTAAAATCGCTATGTCCGCAATTGAGCAACGGGTCATGGTGTTGGCGGACCACCAGGGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCt < 2:817240/231‑1 (MQ=255) atgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAACCACCAGGGCCTGCCAGGCAAAAACTGCCA‑GTAAACGACT‑GGCACCGTAAACCCCGCGTACAGCCAGCACGATGGGGTGGGGGGGAGGCAGTCTGGCAAGGGAATTGAGATCTT‑CCAAACGCCCTGTTTCCGTACCCCCCAAAAGCCcaaa > 1:310098/1‑237 (MQ=255) tgaCATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTccca > 2:692864/1‑238 (MQ=255) ttGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCCGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCCGACTGGCAAGGGGAGTTGGAACTT‑CCCGAAGCCCTGTTTTCGGTCCCGCCAAACACCCACaacaacc > 1:6764/1‑238 (MQ=255) tGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCCCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAAt > 2:46995/1‑239 (MQ=255) ccGCACGGAAGGGCGGTAAAATCGCTATGCCCGAAAAGGAGCAACGGGTCATTTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACg < 2:307889/237‑1 (MQ=255) cGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGCTTCCGTACCCGCAAAACGCTCCCAACGACGGGCAATAACCTCCACGTTa > 2:881368/1‑239 (MQ=255) cACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑TCAGACGCTCTGTTTCCGTACCCGCCAAACGCTCACAACCACGGGCAATAACCCCGCCGTTAtt > 2:535819/1‑239 (MQ=255) cACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCCCCGTTTCCGTACCCGCACAACGCTCACAACGACGGGCAATAACCCCGACGCTAtt > 2:266628/1‑239 (MQ=255) gCGGTAAGATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACG‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGGTGGGGGGAGTCAGTTTGGCAAGGGAATTGAAACCTT‑TCAGACGCTCTGGTTCCGGACCCGCAAAAACATCACAACGACGGGGCATAACCCCCAAGTTTTTGGCTTGaa > 1:537929/1‑237 (MQ=255) gggaaaaTCGGTAAGTTCTCAAATGAGCAACAGGCCATGCTTTTGGCGAGCCACCAGTGCCTGCCAGTCAATATATGCCA‑GAAAACGACC‑GGCACCGTAACCGCCGCGTACAGCCAGCACGCTGGTGTTGGGGGTAGTAAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTTTGTTTCCGTACCCGCAAAACGCTCACACACACGGGCACAAACCTCgcaccatttaactttgtga > 1:741649/4‑220 (MQ=255) tAAAAGCGGTATGTCCGCAAATGAGCACCGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGccc < 2:158923/238‑1 (MQ=255) tATGTCCCAAAATGTACAACGGGTCTTGTTGGTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTa < 2:185456/239‑1 (MQ=255) gCAAAGGAGCACGGGGTCATTGTGTTGGCCAGCCACCAGGGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCt < 2:49859/239‑1 (MQ=255) gCAAAGGAGCAACGGCTCATGTTGGTGGCGAGCCACCAGTGCCGGCCAGTCAATATCTGCAA‑GTAAACGACT‑GGCCACGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCt < 2:56858/239‑1 (MQ=255) tgggCAACGGGTCATGTTGTGGGGGAGCCACCAGTGCCTGCCAGTCAATTTCTGCCA‑GTAAACGACT‑GGAACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATa < 2:853228/236‑1 (MQ=255) aGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTagtcagt > 1:794002/1‑117 (MQ=255) aGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTagtcagt < 2:794002/117‑1 (MQ=255) aGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGTGGCCCCGCGTCGGTAAGGCGCTGGagaa > 2:830726/1‑235 (MQ=255) cGGGTCATTTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCGGCCA‑GTAAACGACT‑GGCCCCGTAACCGGCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGTAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCa < 2:537929/239‑1 (MQ=255) ggTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCCACCAACGACGGGCAATAACCTCGACGTTATTGACTTGATGACCCGCGCCGGGACGGCGCTGGAAACCACGCAg > 1:183422/1‑238 (MQ=255) cATGTTGTTGGCGGGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg < 2:23354/239‑1 (MQ=255) cATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCcgcgcg > 2:91696/1‑239 (MQ=255) cATGTTGTTGGCCACCCCCCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGcgcg < 2:310098/239‑1 (MQ=255) gttgttGGCGACCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGGCT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCgcggcg < 2:781786/239‑1 (MQ=255) ttttgggggcccccctggtcctgccAGTAAAATTCGGCCA‑GTAAACGACT‑GGCACCGTACCCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg < 1:602678/220‑1 (MQ=255) tgttgGCGAGCCACCCGGGCCTGCAAGTCAATATCTGCCA‑GTAACCTTCT‑GGCACCGTACCCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg < 2:278794/239‑1 (MQ=255) tgtggGGGAGCCCCCAGGCCCGGCCAGTCAATCGCTGCCG‑TTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg < 2:857285/239‑1 (MQ=255) gctttgcggggggttttcggtgccAGTTAATTTCTGCCA‑GTAAACCACT‑GGCCGCGTAACCGCCGGGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg < 2:280057/218‑1 (MQ=255) ttgGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGt > 2:48951/1‑236 (MQ=255) aGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTaa > 2:657509/1‑239 (MQ=255) ccggtcccggccAGT‑AAAATCTGGCAGGTAACGGACT‑GGACCGGAAACCGCCGCGTACAGCCAGCACGATGGGGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAg < 1:657509/230‑1 (MQ=255) ccaagggccggacgagaaagttcgCCA‑GAAAACGGGTGGGCACCGGAACCGCCGCGTACAGCCATCGCGGTGGGGTTGGGGGTAGGCAGTCGGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCa < 1:327081/216‑1 (MQ=255) cctgccAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAAccc > 2:598588/1‑239 (MQ=255) gcccggctaagatgtggcA‑GTAACCGGCT‑GGCACCTTAGCCGCCGCGTACAGCCAGCACGATGTGGTTGGGGGTAGGCAGGCTGGCAAGGGAATTGAGATCTT‑CCAGACTCTCTGGTTCCGTACCCGCAAAACGCTCACAACGACGGGCAGTAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGa < 1:565638/222‑1 (MQ=255) ggccagaaatttcgtgacgttAACCGACG‑GGCACTGTACCCGCCGCGTACAGCCAGCACGGTGGTGTTGGGGGTAGTAAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGATCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGa < 1:5150/219‑1 (MQ=255) ccAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTTTTTAATg > 2:278537/1‑226 (MQ=255) attgcgtGCCT‑GTAAACGTCT‑GTCCCCGTAACCGCCGCGTACAGCGAGCACGATTGTGTTGGGGGTAGTCAGTCTGGCAAGGGCATTGAGTTCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCaa < 1:406913/233‑1 (MQ=255) aaTATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGAGGGCGTTGGGGGTAGTCAGTCTGGCAAGGGAGTTGAGATCTT‑CCAGACGCGCTGTTTCCGTACCCGCAAACACCTCACAACGAACGGTCATGACCTCGACGTTTTTGACTTGATGCCCCGCGGCGGTAAGGCGCTGGATACCCAGAAGCGCGGCGTGGCGTTTAATGCCATAACCCCATAGGGaaa > 1:876901/1‑239 (MQ=255) gtggttataagggcagggccT‑GGCCACGTAACGCCCCGGTACAGCCAGCACGATGGTGTTGGGGGTAAGCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCaa < 1:175089/220‑1 (MQ=255) tatCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAAtt < 2:653571/239‑1 (MQ=255) ttaccgcaagaaaccGACG‑GGCCCCGTTCCCGCCGCGTACAGCCAGCACGATGGTGGTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAAtt < 2:479340/225‑1 (MQ=255) gCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGAACTAACCTCGACGTTCTTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCAAGCCGCCCGGCGGGCTGGTTAATGCAGAAACCGGATGGGGCAATTAAGTg > 2:406913/1‑239 (MQ=255) ccA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACAACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGa > 2:827627/1‑239 (MQ=255) ccA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATATT‑CCAGACGCTCTGTTTCCGTACCCGCAAAAGCCTCACAACGACGGGCAATAACCCCGACGTTATTGACTTGATTCCCCGCGTCGGGAAGGCGCTGGTTACCAATCAGGACGCGGGGCTGCTTAATGCAGGAACCCGATGGGGCAATTaaagaa > 1:657965/1‑235 (MQ=255) cGACT‑GGAACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAgaga < 2:869018/239‑1 (MQ=255) cggtgggcccctaaccccccgcGTACAGCCAGCCCGGTGGGGTTGGGGGTAGTCATGCTGGCAAGGGAATGGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAgaga < 1:46995/221‑1 (MQ=255) gCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGc > 2:150268/1‑239 (MQ=255) tAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGGGAAAcctgtctcttatccaaa > 2:175089/1‑222 (MQ=255) caggactgtGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGaaa < 2:639096/230‑1 (MQ=255) ccgaCGGTGGTGTGGGGGGTAGGCAGTCTGGCAAGGGAATTGAGTTCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTGGATGACCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAAct < 1:749235/236‑2 (MQ=255) atcACGATGGTGTTGGGGGTATGCAGTCTGGCAAGGGAATTTAGATCTT‑CCAGTCGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACg < 2:646835/237‑1 (MQ=255) cccGATGGTTGTGGGGGTAGTTAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa < 2:269691/237‑1 (MQ=255) cACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGaa > 2:752875/1‑239 (MQ=255) aTGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTCGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg < 1:594101/158‑1 (MQ=255) aTGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTCGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTg > 2:594101/1‑158 (MQ=255) ggTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGtaa > 2:512047/1‑238 (MQ=255) | GAAATGGTGCTCGGTAAAGGCGTTCAGTTCATCCGCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACCAGTGCCTGCCAGTCAATATCTGCCA‑GTAAACGACT‑GGCACCGTAACCGCCGCGTACAGCCAGCACGATGGTGTTGGGGGTAGTCAGTCTGGCAAGGGAATTGAGATCTT‑CCAGACGCTCTGTTTCCGTACCCGCAAAACGCTCACAACGACGGGCAATAACCTCGACGTTATTGACTTGATGCCCCGCGTCGGTAAGGCGCTGGATACCACGCAGCGCGGCGTGCTGTTTAATGCAGTAACCCGATGGGGCAATTAAGTGAAACAGAGACATGGCAATTCCTTGCTGACAACAGAAACGAAATGTAT > NC_000913/1242643‑1243115 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |