Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,325,980 | A→T | 16.7% | Y412F (TAC→TTC) | yciQ → | enhancer of membrane protein expression, putative inner membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,325,980 | 0 | A | T | 16.7% | 145.7 / 2.3 | 71 | Y412F (TAC→TTC) | yciQ | enhancer of membrane protein expression, putative inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (13/43); new base T (10/3); total (23/48) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.87e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAC > NC_000913/1325743‑1326209 | gttttAAGGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCTCAGAGCAGTTAGTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTAc < 2:110949/236‑1 (MQ=255) aGGTGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa < 2:326261/239‑1 (MQ=255) aGGCGCGAACAGAAATAATGCGGTATTACTGCCCGAAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACtt < 2:895634/237‑1 (MQ=255) aaaaaTAATGGGGTATTACTGCCCGCAGGGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAgg < 1:475154/237‑1 (MQ=255) aataatGGGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCt < 2:169315/238‑1 (MQ=255) aagtcGGTATTACTGCCCGCAGAGCAGTTAATACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc < 2:535000/235‑1 (MQ=255) tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTTCTGTTAATTTGGTTCTGTAgggttgggag > 1:130515/1‑231 (MQ=255) tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTAACTTTAGTCCTGTAAGCTGGGCAg > 2:372410/1‑239 (MQ=255) tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTTAATTGAGGTCTGTatgggggggag > 1:223226/1‑229 (MQ=255) gTATTACTGCCCGCAGGGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGaa < 2:223226/238‑1 (MQ=255) gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTACGGTAACTTTGGTCCTGTGGGCTtggggggaga > 2:110503/1‑232 (MQ=255) gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTCGGTAAATTTAGTCCGCTAGGCTGGGGAGGAAt > 2:469181/1‑239 (MQ=255) gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGGAACATGAGACCTTTAGGCCGGGCAGGAAt > 2:424254/1‑239 (MQ=255) gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGACTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCATTCTTTACGGTAAAttctttctcttatacccatctccgc > 2:65866/1‑215 (MQ=255) tATTGCTGGACGCAGAGAAGTTATTACTGGAAGCGTTATTTCGGAAAGGCGATAAGGTCGTTCTTAAGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTCTTTACGGTAACTTGAGTCCTGTAGGATGGGCAGGAAt < 2:285797/238‑1 (MQ=255) ttACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTTAGTCCTGTAGGCTGGGCAGGAATGAg > 2:414085/1‑239 (MQ=255) cccgcaaggtcgTTATTACTGGAAGCTTTATTTCGGAAAGGCGGAAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGTCATTGTTACTGATGAAGAAATTTTATCTGCCACGTAAAAAGTCGTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCTGTCATTCTTTACGGTAACTGGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggt < 1:898217/228‑1 (MQ=255) cAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAATTTGAGGCCTTTTGGCTTGGCAGGAAAGGGTCTGTTGGGc > 2:333111/1‑239 (MQ=255) gggctgTTTTTTCTGGAAGGGTTTTTTCGTTAAGGCGATAAGGTCGTTTTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa < 1:310835/234‑1 (MQ=255) ggaGTTTTTACTGGAACGGTTTTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa < 1:469181/235‑1 (MQ=255) gCAGTTATTACTGGAAGCGTTAATTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa < 2:753133/237‑1 (MQ=255) cAGTTATTACTGGAAGGGTTATTTCGTAAAGGCGATAAGGTCGGTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGTATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGata < 2:426214/238‑1 (MQ=255) aGTTAATAATGGAATCGTTATGTCGGAAAGGCGATAAGGTCGTTCTTACGGGGAGACGGAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGatat < 2:279055/238‑1 (MQ=255) ttattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGGCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGCCGGCTACTCTTTTTTGTTACTTGTGGCCCGTGAGCTGGGGCGGaaggggatcggtgggggggatgtt > 1:413676/1‑217 (MQ=255) attaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAAt < 2:364553/216‑1 (MQ=255) ttaCTGGAAGGTTTATTTCGTAAAGGCGATAAGGTCTTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTttat < 1:414085/239‑1 (MQ=255) tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTAAGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg < 2:743070/239‑1 (MQ=255) aaGCGTTATTTCGTAAAGGCGTTAAGGTCCTTCTTACGGGGAGCCGCAACAGGGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGGGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGAt < 1:611679/238‑1 (MQ=255) aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTAACTTTGGTCCCGTGGGCTGGGGAGGAATGGGGCTGGTGGGCGGTATGTTTTTTTTGAtt > 2:481130/1‑238 (MQ=255) aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTTTAGGCTTGGCAGGAAATGGTCTTGTggggggttagtttttttttttt > 2:780355/1‑220 (MQ=255) gCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCATCTTTTTGGTTAATTGGGGCCCGTTGGCGGGGGAGGGAAGGGTTTggggggggtgtctttaaaaaaaattgt > 1:364553/1‑218 (MQ=255) ttagggCTATAAGGTCGTTTTTACGGGGAGCCGCACCAGATTTTTACGCAGGGTATTTTTACGGATGCGGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTTCCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTc < 1:774096/235‑1 (MQ=255) aaaGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTc < 2:140047/238‑1 (MQ=255) cGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCtttt < 2:793162/238‑1 (MQ=255) cGTTCTTACGGGGAGACCCAACAGAGTCTTAAGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGtt < 1:118128/239‑1 (MQ=255) gTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCATTCTTTATGGTAAATTGAGTCCTGTGGTCTGGGCGGGGGTGAGTGTTGTGGGGGGTATATTTTTTTTTTTCTTCTG‑GGTTTTTCCtttttttttttttt > 1:14169/1‑238 (MQ=255) ttCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTTAGTCCTGTAGGCTGGGCCGGAATTGGTTTGGGTGGGGATATGTTTTTTTTGGTTTTGTG‑GGTTTTTCCTTTTTTTTTTTTTTc > 2:805379/1‑238 (MQ=255) cggggGACGCAACTGATTCTTTCGCAGGGCTTTTTTACGGGTGCAGAAATTTTAGCAGCCGCGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGc < 1:333111/238‑1 (MQ=255) agaCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTACGGTAAATTTAGTCCTGTAGGGTTGGGCGGGATGAGGCTGGTGGGGGGGATGTTTTTTTTGTATTGCGT‑GGTTATTTCTTTTTTTTTTTTTTTCCtttgggtttt > 1:300537/1‑231 (MQ=255) aCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGCCATTCTTTTCGGTAACTTGAGTCCTGTTGGTTGGGCAGGGAATAGGCTTGTGGGGGGTATGTTTTTTTTGAACTGCCG‑GATTTTTCCTTTTTTTTTGTGTTTCCtttggacttttt > 2:154505/1‑229 (MQ=255) accgattttttccccgggTATTTTTACGGAGGCAGAATTTTTATCTGCCGCGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGcc < 1:481130/224‑1 (MQ=255) tCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGCCATTCTTTACGGGAACTTTGGTCCTGTAGGCTGGGCAGGGATTGGTCTGGGGGGCGGTATGTTTTTTTTGGTCTGCTG‑GATTTTTTCTTTTTTTTTTTTTTCCCTTTAGGTTTTGTTTGTCCCCgat > 2:612765/1‑239 (MQ=255) tacgaggggCATTTTTACGGATGCAGAAATTTTAATTGCCGGGTAAAAAGTCTTCGGTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCgatgat < 2:705112/233‑1 (MQ=255) tACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTTGGTAACTTTGGTTCCGTAGGGCGGGCCGGGATGAGTCTGGTGGGCGGTCTGTTTATTTTGTTTGGCTG‑GGTTTTTCCTTTTTTTATTTTTTCCCTTTTAGTTTTGTTTGtcccggggggg > 1:423365/1‑231 (MQ=255) ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTTTGGTAACTTGGGTCCTGTTGGCTGGGCGGGGATGGGGCTGGGGGGCGGATTGTTTTTTTTTGTTTGGTG‑GGTTTTTCCTTTTTTTTTTTGTTTCCTTTTGCttttttttttcctcgcgtgtgtaaaa > 1:550196/1‑220 (MQ=255) ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGGAAATTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTTCCTTTAGCTTTTGTTTGCCCCCGATGATGGCaaa > 2:15690/1‑237 (MQ=255) ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGATTTACCGGTCTGATACTTTTTTGCAATGGGGGGGACTGGCAATAATGTCGGCTATTCTTTTCTGGTACTTGGGTCACGTTGGCTTGGGCGGGCTGGGTCTGGGTGGGGGGATGTTTTTTTTTATATCCTGTGTTTTTTCTCTTTTTTTTTTTTCCCCATGGGCTTTTGTTTTccccgcgttttgtcca > 1:738162/1‑225 (MQ=255) gttttttttCGGATGGAGAATTTTTATCTGCGGTGTTAAAAGTTTTCGTTTTACCGGTTTGATTTTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGc < 1:424254/235‑1 (MQ=255) gCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTGTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGc < 2:364897/238‑1 (MQ=255) ttttttAGGGATGCAGAAGTTTTATCTGCCGCGTAAAAAGTTTTGGTTTTACAGGTCTGATTCTTTTTTGCAATGGGGTGGACGGGCAATTGTGGCGGTCATTCTTTACGGTAACTTGAGTACTGTATGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTGTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCt < 1:119589/237‑1 (MQ=255) tcgtattaaGAAATTTTATTGGTCGGGTAAAAAGTTTTCGTTTTACCGGTCTGATGCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTa < 1:173307/231‑1 (MQ=255) gacgAAATTTTTTCTGGCGGGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCgt < 1:8777/235‑1 (MQ=255) agcTGCCGGGTAAAAAGGTTTCGTTTTACCGGTGTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACt < 2:748131/237‑1 (MQ=255) aTCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACt < 2:413676/239‑1 (MQ=255) tCTTCGTTTTACCGGTTTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGAt < 2:529134/239‑1 (MQ=255) cTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGAt < 2:274124/238‑1 (MQ=255) ggTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTg < 2:130515/239‑1 (MQ=255) cttgttcTTTTTGGCAATGGGGTTGACTGGCAATTTTGGCGGTCCTTCTTTACGGTAACTTGAGTACTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTAGTATGATCTGCTT‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGc < 2:632583/232‑1 (MQ=255) ttttGCAATGGGGTGGACTGGTAATATTGGGGGTCATTCTTTGCGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATtct < 1:540469/239‑1 (MQ=255) ttGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTCACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTTGTGGGCGATCTGTTTATTATGATCTGGTG‑GGTTATTCCTTTTTTTTTTTTTTTCCCTTAGCTTTTTTTTGTCCCCGGTGATTACAAGCCTTTGGTTTATCCTGTTTTTATTCCTTTTTTTTTTCCACAGTTTTTTTTAGGCGGGGGCttttttttcc > 1:868295/1‑230 (MQ=255) ggggTGGACTGGAAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATc < 2:14169/239‑1 (MQ=255) cTGGAAATTTTGGGGGTTATTCTTTTCGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTTTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCg < 1:35320/238‑1 (MQ=255) tGGGAATTTTGGCGGTCAATCTTTACGGTAACTTGAGTCCTGTAGGGTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCgg < 2:423365/238‑1 (MQ=255) aaTTTTGGGGTTTATTTTTTTCGGTAATTTGAGGCCTTTAGGGTGGGCAGGAATGGGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATg < 1:780355/239‑1 (MQ=255) atatTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATg < 2:868295/238‑1 (MQ=255) ttttgCGGTCTTTTTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTAt < 1:372410/235‑1 (MQ=255) atTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTAt < 2:738162/239‑1 (MQ=255) gttaTTTTTTTCGTTAACTTGTTTCCTGTAGGCTGGGCAGGAATGATTCTGGTGGGCGATATGTTTATTATGATCTTCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCtttt < 1:805379/235‑1 (MQ=255) tCATTGTTTACGGTAACGTTAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTa < 2:216897/238‑1 (MQ=255) taTTGTTTGCGGTAACTTGAGTCCTGTAGGCTGGGCATGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc < 2:468653/237‑1 (MQ=255) cATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc < 2:300537/238‑1 (MQ=255) | GTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTG‑GATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAC > NC_000913/1325743‑1326209 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |