Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,325,980 A→T 16.7% Y412F (TAC→TTC)  yciQ → enhancer of membrane protein expression, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,325,9800AT16.7% 145.7 / 2.3 71Y412F (TAC→TTC) yciQenhancer of membrane protein expression, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (13/43);  new base T (10/3);  total (23/48)
Fisher's exact test for biased strand distribution p-value = 4.87e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAC  >  NC_000913/1325743‑1326209
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      
gttttAAGGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCTCAGAGCAGTTAGTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTAc                                                                                                                                                                                                                                       <  2:110949/236‑1 (MQ=255)
          aGGTGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGa                                                                                                                                                                                                                             <  2:326261/239‑1 (MQ=255)
          aGGCGCGAACAGAAATAATGCGGTATTACTGCCCGAAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACtt                                                                                                                                                                                                                               <  2:895634/237‑1 (MQ=255)
                    aaaaaTAATGGGGTATTACTGCCCGCAGGGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAgg                                                                                                                                                                                                                   <  1:475154/237‑1 (MQ=255)
                       aataatGGGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCt                                                                                                                                                                                                                 <  2:169315/238‑1 (MQ=255)
                          aagtcGGTATTACTGCCCGCAGAGCAGTTAATACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGc                                                                                                                                                                                                             <  2:535000/235‑1 (MQ=255)
                            tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTTCTGTTAATTTGGTTCTGTAgggttgggag                                                                                                                                                                                                           >  1:130515/1‑231 (MQ=255)
                            tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTAACTTTAGTCCTGTAAGCTGGGCAg                                                                                                                                                                                                           >  2:372410/1‑239 (MQ=255)
                            tGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTTAATTGAGGTCTGTatgggggggag                                                                                                                                                                                                           >  1:223226/1‑229 (MQ=255)
                                gTATTACTGCCCGCAGGGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGaa                                                                                                                                                                                                        <  2:223226/238‑1 (MQ=255)
                                gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTACGGTAACTTTGGTCCTGTGGGCTtggggggaga                                                                                                                                                                                                       >  2:110503/1‑232 (MQ=255)
                                gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTCGGTAAATTTAGTCCGCTAGGCTGGGGAGGAAt                                                                                                                                                                                                       >  2:469181/1‑239 (MQ=255)
                                gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGGAACATGAGACCTTTAGGCCGGGCAGGAAt                                                                                                                                                                                                       >  2:424254/1‑239 (MQ=255)
                                gTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGACTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCATTCTTTACGGTAAAttctttctcttatacccatctccgc                                                                                                                                                                                                        >  2:65866/1‑215 (MQ=255)
                                 tATTGCTGGACGCAGAGAAGTTATTACTGGAAGCGTTATTTCGGAAAGGCGATAAGGTCGTTCTTAAGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTCTTTACGGTAACTTGAGTCCTGTAGGATGGGCAGGAAt                                                                                                                                                                                                       <  2:285797/238‑1 (MQ=255)
                                   ttACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTTAGTCCTGTAGGCTGGGCAGGAATGAg                                                                                                                                                                                                    >  2:414085/1‑239 (MQ=255)
                                         cccgcaaggtcgTTATTACTGGAAGCTTTATTTCGGAAAGGCGGAAAGGGCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGTCATTGTTACTGATGAAGAAATTTTATCTGCCACGTAAAAAGTCGTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCTGTCATTCTTTACGGTAACTGGAGTCCTGTAGGCTGGGCAGGAATGAGTCtggt                                                                                                                                                                                              <  1:898217/228‑1 (MQ=255)
                                             cAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAATTTGAGGCCTTTTGGCTTGGCAGGAAAGGGTCTGTTGGGc                                                                                                                                                                                          >  2:333111/1‑239 (MQ=255)
                                               gggctgTTTTTTCTGGAAGGGTTTTTTCGTTAAGGCGATAAGGTCGTTTTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa                                                                                                                                                                                        <  1:310835/234‑1 (MQ=255)
                                                 ggaGTTTTTACTGGAACGGTTTTTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa                                                                                                                                                                                        <  1:469181/235‑1 (MQ=255)
                                                 gCAGTTATTACTGGAAGCGTTAATTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGa                                                                                                                                                                                        <  2:753133/237‑1 (MQ=255)
                                                  cAGTTATTACTGGAAGGGTTATTTCGTAAAGGCGATAAGGTCGGTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGTATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGata                                                                                                                                                                                      <  2:426214/238‑1 (MQ=255)
                                                   aGTTAATAATGGAATCGTTATGTCGGAAAGGCGATAAGGTCGTTCTTACGGGGAGACGGAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGatat                                                                                                                                                                                     <  2:279055/238‑1 (MQ=255)
                                                     ttattaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGGCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGCCGGCTACTCTTTTTTGTTACTTGTGGCCCGTGAGCTGGGGCGGaaggggatcggtgggggggatgtt                                                                                                                                                                                  >  1:413676/1‑217 (MQ=255)
                                                       attaCTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAAt                                                                                                                                                                                                       <  2:364553/216‑1 (MQ=255)
                                                        ttaCTGGAAGGTTTATTTCGTAAAGGCGATAAGGTCTTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTttat                                                                                                                                                                               <  1:414085/239‑1 (MQ=255)
                                                            tGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTAAGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATg                                                                                                                                                                           <  2:743070/239‑1 (MQ=255)
                                                               aaGCGTTATTTCGTAAAGGCGTTAAGGTCCTTCTTACGGGGAGCCGCAACAGGGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGGGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGAt                                                                                                                                                                         <  1:611679/238‑1 (MQ=255)
                                                               aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTTCGGTAACTTTGGTCCCGTGGGCTGGGGAGGAATGGGGCTGGTGGGCGGTATGTTTTTTTTGAtt                                                                                                                                                                        >  2:481130/1‑238 (MQ=255)
                                                               aaGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTTTAGGCTTGGCAGGAAATGGTCTTGTggggggttagtttttttttttt                                                                                                                                                                        >  2:780355/1‑220 (MQ=255)
                                                                 gCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCATCTTTTTGGTTAATTGGGGCCCGTTGGCGGGGGAGGGAAGGGTTTggggggggtgtctttaaaaaaaattgt                                                                                                                                                                      >  1:364553/1‑218 (MQ=255)
                                                                            ttagggCTATAAGGTCGTTTTTACGGGGAGCCGCACCAGATTTTTACGCAGGGTATTTTTACGGATGCGGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTTCCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTc                                                                                                                                                          <  1:774096/235‑1 (MQ=255)
                                                                             aaaGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTc                                                                                                                                                          <  2:140047/238‑1 (MQ=255)
                                                                                  cGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCtttt                                                                                                                                                     <  2:793162/238‑1 (MQ=255)
                                                                                           cGTTCTTACGGGGAGACCCAACAGAGTCTTAAGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGtt                                                                                                                                           <  1:118128/239‑1 (MQ=255)
                                                                                            gTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGGCATTCTTTATGGTAAATTGAGTCCTGTGGTCTGGGCGGGGGTGAGTGTTGTGGGGGGTATATTTTTTTTTTTCTTCTGGGTTTTTCCtttttttttttttt                                                                                                                                           >  1:14169/1‑238 (MQ=255)
                                                                                             ttCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTTAGTCCTGTAGGCTGGGCCGGAATTGGTTTGGGTGGGGATATGTTTTTTTTGGTTTTGTGGGTTTTTCCTTTTTTTTTTTTTTc                                                                                                                                          >  2:805379/1‑238 (MQ=255)
                                                                                                    cggggGACGCAACTGATTCTTTCGCAGGGCTTTTTTACGGGTGCAGAAATTTTAGCAGCCGCGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGc                                                                                                                                  <  1:333111/238‑1 (MQ=255)
                                                                                                        agaCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTACGGTAAATTTAGTCCTGTAGGGTTGGGCGGGATGAGGCTGGTGGGGGGGATGTTTTTTTTGTATTGCGTGGTTATTTCTTTTTTTTTTTTTTTCCtttgggtttt                                                                                                                              >  1:300537/1‑231 (MQ=255)
                                                                                                          aCGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGCCATTCTTTTCGGTAACTTGAGTCCTGTTGGTTGGGCAGGGAATAGGCTTGTGGGGGGTATGTTTTTTTTGAACTGCCGGATTTTTCCTTTTTTTTTGTGTTTCCtttggacttttt                                                                                                                            >  2:154505/1‑229 (MQ=255)
                                                                                                               accgattttttccccgggTATTTTTACGGAGGCAGAATTTTTATCTGCCGCGTAAAAAGTTTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGcc                                                                                                                       <  1:481130/224‑1 (MQ=255)
                                                                                                                     tCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGCCATTCTTTACGGGAACTTTGGTCCTGTAGGCTGGGCAGGGATTGGTCTGGGGGGCGGTATGTTTTTTTTGGTCTGCTGGATTTTTTCTTTTTTTTTTTTTTCCCTTTAGGTTTTGTTTGTCCCCgat                                                                                                                 >  2:612765/1‑239 (MQ=255)
                                                                                                                        tacgaggggCATTTTTACGGATGCAGAAATTTTAATTGCCGGGTAAAAAGTCTTCGGTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCgatgat                                                                                                              <  2:705112/233‑1 (MQ=255)
                                                                                                                        tACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTTTTTTTGGTAACTTTGGTTCCGTAGGGCGGGCCGGGATGAGTCTGGTGGGCGGTCTGTTTATTTTGTTTGGCTGGGTTTTTCCTTTTTTTATTTTTTCCCTTTTAGTTTTGTTTGtcccggggggg                                                                                                              >  1:423365/1‑231 (MQ=255)
                                                                                                                               ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCCTTTTTTTTGGTAACTTGGGTCCTGTTGGCTGGGCGGGGATGGGGCTGGGGGGCGGATTGTTTTTTTTTGTTTGGTGGGTTTTTCCTTTTTTTTTTTGTTTCCTTTTGCttttttttttcctcgcgtgtgtaaaa                                                                                                        >  1:550196/1‑220 (MQ=255)
                                                                                                                               ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGGAAATTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTTCCTTTAGCTTTTGTTTGCCCCCGATGATGGCaaa                                                                                                        >  2:15690/1‑237 (MQ=255)
                                                                                                                               ggCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGATTTACCGGTCTGATACTTTTTTGCAATGGGGGGGACTGGCAATAATGTCGGCTATTCTTTTCTGGTACTTGGGTCACGTTGGCTTGGGCGGGCTGGGTCTGGGTGGGGGGATGTTTTTTTTTATATCCTGTGTTTTTTCTCTTTTTTTTTTTTCCCCATGGGCTTTTGTTTTccccgcgttttgtcca                                                                                                         >  1:738162/1‑225 (MQ=255)
                                                                                                                                gttttttttCGGATGGAGAATTTTTATCTGCGGTGTTAAAAGTTTTCGTTTTACCGGTTTGATTTTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGc                                                                                                       <  1:424254/235‑1 (MQ=255)
                                                                                                                                gCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTGTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGc                                                                                                       <  2:364897/238‑1 (MQ=255)
                                                                                                                                  ttttttAGGGATGCAGAAGTTTTATCTGCCGCGTAAAAAGTTTTGGTTTTACAGGTCTGATTCTTTTTTGCAATGGGGTGGACGGGCAATTGTGGCGGTCATTCTTTACGGTAACTTGAGTACTGTATGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTGTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCt                                                                                                     <  1:119589/237‑1 (MQ=255)
                                                                                                                                        tcgtattaaGAAATTTTATTGGTCGGGTAAAAAGTTTTCGTTTTACCGGTCTGATGCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTa                                                                                              <  1:173307/231‑1 (MQ=255)
                                                                                                                                              gacgAAATTTTTTCTGGCGGGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCgt                                                                                         <  1:8777/235‑1 (MQ=255)
                                                                                                                                                         agcTGCCGGGTAAAAAGGTTTCGTTTTACCGGTGTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACt                                                                             <  2:748131/237‑1 (MQ=255)
                                                                                                                                                         aTCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACt                                                                             <  2:413676/239‑1 (MQ=255)
                                                                                                                                                                          tCTTCGTTTTACCGGTTTGATTCTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGAt                                                            <  2:529134/239‑1 (MQ=255)
                                                                                                                                                                           cTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGAt                                                            <  2:274124/238‑1 (MQ=255)
                                                                                                                                                                                       ggTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTg                                               <  2:130515/239‑1 (MQ=255)
                                                                                                                                                                                          cttgttcTTTTTGGCAATGGGGTTGACTGGCAATTTTGGCGGTCCTTCTTTACGGTAACTTGAGTACTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTAGTATGATCTGCTTGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGc                                             <  2:632583/232‑1 (MQ=255)
                                                                                                                                                                                                   ttttGCAATGGGGTGGACTGGTAATATTGGGGGTCATTCTTTGCGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATtct                                   <  1:540469/239‑1 (MQ=255)
                                                                                                                                                                                                     ttGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTCACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTTGTGGGCGATCTGTTTATTATGATCTGGTGGGTTATTCCTTTTTTTTTTTTTTTCCCTTAGCTTTTTTTTGTCCCCGGTGATTACAAGCCTTTGGTTTATCCTGTTTTTATTCCTTTTTTTTTTCCACAGTTTTTTTTAGGCGGGGGCttttttttcc                                  >  1:868295/1‑230 (MQ=255)
                                                                                                                                                                                                            ggggTGGACTGGAAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATc                          <  2:14169/239‑1 (MQ=255)
                                                                                                                                                                                                                    cTGGAAATTTTGGGGGTTATTCTTTTCGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTTTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCg                   <  1:35320/238‑1 (MQ=255)
                                                                                                                                                                                                                     tGGGAATTTTGGCGGTCAATCTTTACGGTAACTTGAGTCCTGTAGGGTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCgg                  <  2:423365/238‑1 (MQ=255)
                                                                                                                                                                                                                         aaTTTTGGGGTTTATTTTTTTCGGTAATTTGAGGCCTTTAGGGTGGGCAGGAATGGGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATg             <  1:780355/239‑1 (MQ=255)
                                                                                                                                                                                                                          atatTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATg             <  2:868295/238‑1 (MQ=255)
                                                                                                                                                                                                                            ttttgCGGTCTTTTTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTAt          <  1:372410/235‑1 (MQ=255)
                                                                                                                                                                                                                            atTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTAt          <  2:738162/239‑1 (MQ=255)
                                                                                                                                                                                                                                   gttaTTTTTTTCGTTAACTTGTTTCCTGTAGGCTGGGCAGGAATGATTCTGGTGGGCGATATGTTTATTATGATCTTCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCtttt    <  1:805379/235‑1 (MQ=255)
                                                                                                                                                                                                                                    tCATTGTTTACGGTAACGTTAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTa   <  2:216897/238‑1 (MQ=255)
                                                                                                                                                                                                                                     taTTGTTTGCGGTAACTTGAGTCCTGTAGGCTGGGCATGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc  <  2:468653/237‑1 (MQ=255)
                                                                                                                                                                                                                                     cATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAc  <  2:300537/238‑1 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                      
GTGTTAATGAAGGCGCGACCAGAAATAATGCGGTATTACTGCCCGCAGAGCAGTTATTACTGGAAGCGTTATTTCGTAAAGGCGATAAGGTCGTTCTTACGGGGAGACGCAACAGAGTCTTACGCAGGGCATTTTTACGGATGCAGAAATTTTATCTGCCGCGTAAAAAGTCTTCGTTTTACCGGTCTGATACTTTTTTGCAATGGGGTGGACTGGCAATATTGGCGGTCATTCTTTACGGTAACTTGAGTCCTGTAGGCTGGGCAGGAATGAGTCTGGTGGGCGATATGTTTATTATGATCTGCTGGATTATTCCTTTTTTATTTTGTTCCCTTGAGCTTTTGTTTGCCCGCGATGATGACAAGCCTTGCGTTAATCGTGTAATCATCACTTTGTTTTTACCACTGATTTGTTCAGGCGTGGCCTTCTATTCTCTCTACATCAATGTCGGAGATGTATTCTTTTAC  >  NC_000913/1325743‑1326209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: