Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,383,720 G→A 15.4% S81F (TCT→TTT)  ycjW ← LacI family putative transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,383,7200GA15.4% 129.4 / 2.1 66S81F (TCT→TTT) ycjWLacI family putative transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base G (43/11);  new base A (3/8);  total (47/19)
Fisher's exact test for biased strand distribution p-value = 1.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAATAGTA  >  NC_000913/1383488‑1383953
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCCTTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCCGCTTTGGCGGTGATTGCATGCAAAACTTCAGGaaaaa                                                                                                                                                                                                                                         >  1:615087/1‑239 (MQ=255)
ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTTTAATATACCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCAAGCAAAACTTCAGGaaaaa                                                                                                                                                                                                                                         >  1:779213/1‑239 (MQ=255)
ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaa                                                                                                                                                                                                                                          <  2:109612/239‑1 (MQ=255)
ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAAGGaaaa                                                                                                                                                                                                                                          >  2:101414/1‑239 (MQ=255)
ggCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaaaa                                                                                                                                                                                                                                         >  2:576300/1‑239 (MQ=255)
 gtaatttggcgcgcAAATTTGGCAAACCCCCACACCGTAAAAACATATTTAACGGGTTGGTGAAAAAATGACTCACCCGGTCGCGAACTGAGCAAAATAATGCCGTTAATCATTGTCTGCTTGATTTTGCTTTCGCATTATTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAAGATCACGTCGAAACCTTTTCCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGaaat                                                                                                                                                                                                                                          <  1:876428/225‑2 (MQ=255)
           cTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGGGGTGTTGGCATGCAAAACTTCCGTGAAAAACGGATTAcc                                                                                                                                                                                                                               >  2:860136/1‑238 (MQ=255)
           cTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGCGAAAAACGGATTAcc                                                                                                                                                                                                                               >  2:75375/1‑238 (MQ=255)
           cTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGCTTAccc                                                                                                                                                                                                                              >  2:895305/1‑239 (MQ=255)
           cTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATAACACATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATAATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTTTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGGTGGCATGCAAAAATTCAGGAAAAAACGGATAAcc                                                                                                                                                                                                                               >  1:580351/1‑238 (MQ=255)
              cAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCATCCAAAACTTCAGAGAAAAACGGATTAcccccc                                                                                                                                                                                                                           >  2:36968/1‑239 (MQ=255)
               aaCTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTTTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCATGCAAAACTTCAGAGAAAAAAAGATTACccgccg                                                                                                                                                                                                                          >  1:691890/1‑239 (MQ=255)
                   ttGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCCAAACTTCAGGGAAAAACGGATTACCCGCCGTAGt                                                                                                                                                                                                                      >  2:641509/1‑239 (MQ=255)
                    tGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGGGGTGGCATGCAAAACTTTCAAAAAAAACGGGTTACCCGCCGGAGtt                                                                                                                                                                                                                     >  1:678136/1‑239 (MQ=255)
                     gCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCAATCAAAACTTTAGAGAAAAACGGTTTACCCCCCGCAGttt                                                                                                                                                                                                                    >  1:80512/1‑239 (MQ=255)
                                ccaccGGAATATCATTTTTAACGAGTTGGGAAAAAAATGCCTCACCCGCTGGCGACCTGAGCATATTAATGCTTTTAATCATTTTCTGTTTAATTTTGCTTCCGCATTTTGGCAGATTTTCGGCCGGATTGGGGGACGTTTTTAATATCACGTCGAACCCTTCTTCTTCAGCTTTGGCGGTGATGGAAGGAAAAACTTAAGAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg                                                                                                                                                                                                        <  2:863133/239‑1 (MQ=255)
                                      gAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGTTCGAACGGGCAGaa                                                                                                                                                                                                  >  2:43203/1‑239 (MQ=255)
                                      gAATATAATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGCACTGAGCATAATAATGCCTTTAATCATTTTCAGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTACTATCCAGTCGAAAACTTCTTCTTCAGCTTTGTCGGTCAGGCACTTCAAAATCTCGTGGCAAAACGGACTACACCCCGTAGTTTTGGTGGacacggcagaa                                                                                                                                                                                                   >  1:289040/1‑229 (MQ=255)
                                         atattatttttttgggTTTGGAAAAAAATGCACTCTCCCGTGGGGAACCTGGGCATAAAAATCCTTTTAATTTTTTTCTTTTTATTTTTTCTTTCGCTTTTTTGCAGATCTTCTGCGGGTTTGTGGGCTGTTGGTAATATCACGTCGAACCCTTCTTCTTCAGCTTGGGCGGTGATGGCATGCAAAACTTCAAAAAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAAt                                                                                                                                                                                                <  1:101414/224‑1 (MQ=255)
                                           tCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATTACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGTTTAACCGCCGGAGTTTTGGTAGAAAGGGGACAAATCAc                                                                                                                                                                                             >  1:626806/1‑239 (MQ=255)
                                           tCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTGGAAATcc                                                                                                                                                                                              >  2:895955/1‑237 (MQ=255)
                                              tatTCATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGGAGTTTTGGTCGAACGGGTAGaaacaggc                                                                                                                                                                                            >  2:594041/1‑232 (MQ=255)
                                                 ttATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTACTTAATTTTGCTTTCGAATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATAACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGTTGGCATGCAAACTTTCAGAGGAAAACGGATACACCACCGTTGTTTTGGGGGAAAGGGGAAAAAACaaccaaaa                                                                                                                                                                                       >  1:320842/1‑232 (MQ=255)
                                                   aTCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGGGATGGCATGCAAAACTTCAAAGAAAAACGGATTACCCGCCGCAGTTTTGGTCGAAAGGGTAAAAAacacaaaaaaag                                                                                                                                                                                     >  1:208990/1‑231 (MQ=255)
                                                         ttGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATAACCATAATGGCATCaa                                                                                                                                                                               >  2:650533/1‑239 (MQ=255)
                                                            ggCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGGGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaaa                                                                                                                                                                             >  2:758791/1‑238 (MQ=255)
                                                                        aCTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGCAGAGGTc                                                                                                                                                                 >  2:67328/1‑239 (MQ=255)
                                                                        aCTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCCCCGTAGTTTTGTTCGAACGGGTAAAAATCACCCTAATGGCAACAAAACCCCAAAAggga                                                                                                                                                                 >  1:784748/1‑237 (MQ=255)
                                                                            aTCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGCTTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAACATTCAGAGAAAAACGGATTACCCTCCCGAGTTTTGGTCGACCGGGTGGCAATAACCATAAATGCACAAAAACCCaaaagggaaaaca                                                                                                                                                             >  1:491077/1‑229 (MQ=255)
                                                                                      gAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGACCGGGGTAGAAATACCAAAATGGCCTCAAAACCCCAAAAGGCCAACGCGGGGGCCAg                                                                                                                                                 >  1:741725/1‑239 (MQ=255)
                                                                                                       tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGCTGGCATGCAAAACTTCAGAGAAAAACCGATTACCCGCCGGCGTTTTGGTCGAACCGGTAGAAATCAACACAATGGCTCCAAAAACCCAAGAGGTACGCGGGGGGGCAACCTTGGTTAGCTgcaca                                                                                                                                 >  1:30213/1‑235 (MQ=255)
                                                                                                       tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTGGGTTTTGTCGAACGGGTAGAAATCACCCTAATGGCATCAAAAACCGAAGAGGGCGCGGGGCGGGCCAGCGTGTTGGGCGGATaa                                                                                                                                 >  1:755104/1‑238 (MQ=255)
                                                                                                       tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCCTAAAGGCATCAAAACCCGAAGAGGCAAAGGCGCGGGACAACTTGTTTGGCTGAAAc                                                                                                                                 >  1:463026/1‑239 (MQ=255)
                                                                                                       tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAAAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTgct                                                                                                                                   >  2:294638/1‑235 (MQ=255)
                                                                                                       tGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAAAGGTCAGGCGACAGAGCACCTTCTTTGGCTGATAc                                                                                                                                 >  2:826559/1‑239 (MQ=255)
                                                                                                         cttttAAACTTTTCTTGTTTATTTTTTGTTTCGTTTTTTTGAAGTTTTTCTGCGGGTTTGTGCGTCTTTTTAAAAATCACGGCGAAACCTTCTTCTTCAGTTTTGGCGGTAATGGCATGAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTg                                                                                                                               <  2:691890/237‑1 (MQ=255)
                                                                                                         ccTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCCTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAACAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGTTACTg                                                                                                                               >  2:69128/1‑239 (MQ=255)
                                                                                                            ttAATTATTCTGTGGTAAATTTTTCTTTCGCTTTTTTGCAGATTTTCTGCGGGTTTGGGCAACTTTTTAAATACCACGTCAAACCCTTCTTCTTCAGCTTGGGGGGTGATGGAAGGCAAACCTTAAGAGAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTaa                                                                                                                            <  2:741725/239‑1 (MQ=255)
                                                                                                            ttAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTAAGCGCGCGGGCCAGCTTGTTTGGCTGAAAcaggaa                                                                                                                            >  1:68303/1‑234 (MQ=255)
                                                                                                              aaTCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCCCGGGCCAGCTTGTTTGGCTGTAACAGTAAtt                                                                                                                          >  2:319407/1‑239 (MQ=255)
                                                                                                                   ttttCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACATCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATAACCATAATGGCATCAAAACCCGAAGAGGCCAGCGGGCGGGCCAGCTTGTTTGGCTGAAACTTGAATTTTTCa                                                                                                                     >  1:326086/1‑239 (MQ=255)
                                                                                                                          cTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCCGAGTTTTGGTCGAACGGTGAGAAATCACCACAATGGCCTCAAAACCCCAAAAGGTCACGCACCGGGCCCACTGTGTTGGCTGGAACTGAAATTCTTACATGGccc                                                                                                              >  1:409180/1‑239 (MQ=255)
                                                                                                                            tAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTTTAATTCTTCAATTGCCCgg                                                                                                            >  2:153124/1‑238 (MQ=255)
                                                                                                                                       tCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCACAACCCGAAGAGGTCAGCGGCCGGGCCCGCTTGTTTGGTGGATACTGTAATTCTTCAATGGCCCGGAAAACtttttt                                                                                                >  1:664902/1‑238 (MQ=255)
                                                                                                                                                                 aTTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGCTTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGCAAAGGGCAGAGCGCGGGCCCGCTTGTTTGGGTGATACTGTAATTTTTCAATGGCCCGGAACACATTTTCCCGCGCTTCCGGGGAGGTATTAAttt                                                                      >  1:642940/1‑239 (MQ=255)
                                                                                                                                                                     tgCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGCAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTACCACTTTTTCGCGCGCTTCCGGGGAGGTATTAGTTTGCtt                                                                  >  2:8559/1‑239 (MQ=255)
                                                                                                                                                                      gCGACGTTTGTAATATCGCGGCAAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAACCTGCAGAAAAAAACGGTTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTa                                                                 <  2:678136/239‑1 (MQ=255)
                                                                                                                                                                             tggtaaacacACGGCAAACCCTTTTTCTACGGCTTGGGGGGGGTTGGAATGAAAAACTAAAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGc                                                           <  2:784748/229‑1 (MQ=255)
                                                                                                                                                                                     tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGt                                                                                                                                                                  >  2:39231/1‑130 (MQ=255)
                                                                                                                                                                                        cGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCaa                                                                                                                    >  2:686554/1‑171 (MQ=255)
                                                                                                                                                                                        cGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGGGATACg                                               >  2:815219/1‑239 (MQ=255)
                                                                                                                                                                                        cGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGGAACACTTTTTTTGCGCCTTCCGGGGGGATATTAATTTGGTTTTTCAGCAcgcgggcaagg                                               >  1:80482/1‑232 (MQ=255)
                                                                                                                                                                                           cGAACCTTTCTTCTCCTCTTTGGGGGGTGATGGCAGCAAAAATTGAAAAGAAAGACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGt                                             <  2:615087/238‑1 (MQ=255)
                                                                                                                                                                                                     ttctccAGCTTGGGGGTTGAGGGTATCAAAACCTACAGAGAAAACCGTATGACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGGTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACCCTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATa                                  <  2:715918/239‑1 (MQ=255)
                                                                                                                                                                                                           aGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCCCCGTAGTTTTGGTCGAACCGGTTGAAATCACCATAATGGCATCAAAACCCGCAGAGGTCACCGCGCGGGCCAGCTTGTTTGGCTGGTACTGTAATTCTTCAATGGCCCCGACAACTTTTTCGCGGGCTCCCGGGGGGGTATTAAGTTGCTTATTCAGCACGCTGGAAACCGTGGTTTTTGGTAAGCCt                             >  1:197398/1‑238 (MQ=255)
                                                                                                                                                                                                            gCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTACCACCTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGGGATACGGGGGATTTTGGTACGCCTGc                           >  1:213304/1‑239 (MQ=255)
                                                                                                                                                                                                                tgggggggaggtcATGCAAAACTGCAAAAAAAAACGGATTCCCCGCCGTAGGTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAACCCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACTCTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAAcc                       <  2:352831/227‑1 (MQ=255)
                                                                                                                                                                                                                     gTGATGGCATGAAAAACTTCAGAAAAAAGCGGTTTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTgg                   <  2:326086/238‑1 (MQ=255)
                                                                                                                                                                                                                       gagggaaTGAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa                 <  2:554025/232‑1 (MQ=255)
                                                                                                                                                                                                                       gATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCCGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTTGCGCGCTTCCGGGGGGGTTTTAGTTTGCTTATTCCGACCGCGTGATACGGGGGATTTTGATAACCCTGCAACCCTGGCaa                >  1:554025/1‑239 (MQ=255)
                                                                                                                                                                                                                        gtggccaagaaaaaCTTCAGAAAAAACTGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa                 <  2:580351/228‑1 (MQ=255)
                                                                                                                                                                                                                        ggggaaattgaaaaCTGAAAAGAAAAACGGTTGCCCCGCCGGAGGTTTGGTCGGACGGGTCAAAATACCCATAATGGAATCAAACCCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa                 <  1:100592/228‑1 (MQ=255)
                                                                                                                                                                                                                         gatggaaggaaaatcgttagaaAAACACGGTGTACCCGCCTTAGTTTTGGCCGATCGGGTAGAAATCACCCTAATGGCATCAAAACCCGAGGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa                 <  2:409180/220‑1 (MQ=255)
                                                                                                                                                                                                                         gatggaagaaaaaCTTAAAAAAAAAAGGGTATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCa                 <  2:68303/229‑1 (MQ=255)
                                                                                                                                                                                                                             aataaaaaaCTTCAAAAAAAAACGAATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATc            <  2:80512/233‑1 (MQ=255)
                                                                                                                                                                                                                                 acaaaatcgcAGAAAAACACGGATCTCCCGCCTTAGTTTTGGGCGAACGGGTAGGAAGCCACATAATGGCCTCAAAACCCGAAGATGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATTCTGTAATTTTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATa         <  2:851291/229‑1 (MQ=255)
                                                                                                                                                                                                                                      tttCAAAAAAAAAGGGATTACCCGCCGTAGTTTTGGTGGAACGGGTAGAAATCGCCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAAtagta  <  1:576300/238‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              
GGCATATTGACCTTCAACTTTGCCAATCACCACCACCGGAATATCATATTTATCGAGTTGGGCAAAAAATGACTCATCCGCTGGCGAACTGAGCATAATAATGCCTTTAATCATTTTCTGCTTAATTTTGCTTTCGCATTTTTGCAGATCTTCTGCCGGATTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTTTGGCTGATACTGTAATTCTTCAATGGCCCGTAACACTTTTTCGCGCGCTTCCGGGGAGATATTAGTTTGCTTATTCAGCACGCGTGATACGGTGGATTTTGATACGCCTGCAACCCTGGCAATATCATAAATAGTA  >  NC_000913/1383488‑1383953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: