| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,536,735 | A→C | 15.0% | V192G (GTG→GGG) | narW ← | nitrate reductase 2 (NRZ), delta subunit (assembly subunit) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,536,735 | 0 | A | C | 15.0% | 185.5 / 5.3 | 78 | V192G (GTG→GGG) | narW | nitrate reductase 2 (NRZ), delta subunit (assembly subunit) |
| Reads supporting (aligned to +/- strand): ref base A (42/20); new base C (3/12); total (45/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATA > NC_000913/1536499‑1536973 | gaggagGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGCCACACGCCGTGGCGTTATCTTCATTAAA‑CTTCA‑cc > 2:300985/1‑239 (MQ=255) ggtccgcCAGGTGTACTGCCCGTAGTCGTAGAGCAGCCAGCTGCCGAGGAAAAACCCCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt < 2:482283/234‑1 (MQ=255) ggCGCGCCAAGTGTCTCGCCCGTAGGCGTAGCCCCGACAGCCGGCGAGGAAAAACACCAGCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCCCCTCCCGCCCTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt < 1:355738/238‑1 (MQ=255) ggCGCCACAGGTGTACTGCCCGTAGTTGTAGCGGCGCCAGGTGCCGAGGAAAAACCCCGTCGCAAAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCACCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGtt < 1:625843/238‑1 (MQ=255) ccggcaaggggttcgtcccGGAGTCGTAGGCCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATAACGTAAAAAAAGACGTTCAGATACTGAAGCATTCCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTcc < 1:105794/222‑1 (MQ=255) ccAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGAAATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCCGCGAGCTGTCACACCCCGTGGGGTTATCTTCAAACAA‑CTTCC‑CCTGTTCCTCtt > 1:585875/1‑238 (MQ=255) gCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTAC‑CCTGTTCCTCTTCCCAAACTGCa > 2:798223/1‑238 (MQ=255) gCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CCTCA‑CCTGTTCCTCTTCCCACACTGCAt > 2:114106/1‑239 (MQ=255) cccgttgtgTAGCCCAGCCAGCTGGCAAGGAAAACCCCCCTCGCACAAATGTACGGGTATATATCGGAAAAAAAGCCGGTCAGATCCTGAATCATTTCCCTCCTCCCGCACTGATGTCGACATATCGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTACGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCa < 1:444387/231‑1 (MQ=255) tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGCCATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACCCTGCATCAAGCGCCTgg > 2:297144/1‑239 (MQ=255) tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGCTATTGATTAAGCGGCGAGCTGTCACACGCCCTGGCGTTATCTTCAATAAA‑CTTCA‑CCTTTTCCTCTTCCCACCCCCCCTCAAGCCCCTgg > 1:56443/1‑239 (MQ=255) tAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACCCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAgccgcggg > 1:433688/1‑233 (MQ=255) gccagcTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGGGCTGTCACACGCCGGGGGGTTATTTTCAATAAA‑CTTAA‑CCTGTTCCTCTTCCCACCCTGCATCAAGACCCCGGCggggg > 1:768700/1‑236 (MQ=255) gccagcTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGGGCCGTTATCTTAAATAAA‑CTTAA‑CCTGTTCCTCTTCCCCCACGCAATCAAGCGCCTgcgggggt > 1:863045/1‑231 (MQ=255) tGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGCCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTGC‑CCTGTTCCCTCTCCCCCACTGCATCAACCCCCTGCCGGGGGGCAAcgc > 1:649360/1‑239 (MQ=255) aaaaaCACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATTCTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCtt < 2:357845/237‑1 (MQ=255) aaacccccGTTGCAAAAATGTGCGGGGAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCC‑CCTGTGCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATAGCGCTCTTCg < 1:96499/232‑1 (MQ=255) gTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCCCCCCCTTCCCTGttt > 1:146848/1‑236 (MQ=255) gTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGGCATCTTCCCTGTTTACt > 2:747577/1‑239 (MQ=255) aaTGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGGCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTtgt < 2:146848/239‑1 (MQ=255) ttcgggtaatatgcGTAAAAAAAGACTTTGAGAAAGGGGATGATTGCCCACCACCCGAGAGGATGGCGACATATTGCGGCGCGACAGCATGGCTAAAGCGTCTCTGATAATGAGTAAGCGGCGAGCTGGCACACGCCGAGGCGTTATCTTCAATAAA‑CTTCG‑CCGGTACCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGaca < 1:300985/226‑1 (MQ=255) aCGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGacac < 2:650605/239‑1 (MQ=255) cGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTTCATCAAGCGCCTGCCGGGTGTCATCCCCCCCTTCCCGGTTTCCTTTTTTtgggca > 1:143335/1‑232 (MQ=255) tAGATATCGTAAAAAAATGCGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGGCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc < 2:863045/238‑1 (MQ=255) tAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc < 2:56443/238‑1 (MQ=255) tAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTc < 2:768700/238‑1 (MQ=255) atatCGTAAAAAAAGCGCTTCAGATACTGAATCATTTCCCTCCCCCCGCATGGATGTCGAAATATTGCGGCGCGACATCCGGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACt < 1:482517/238‑1 (MQ=255) atatCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAACCCCTGGCGGGGGTCATCCCCCCTTTCCCCGTTTTCTtttttttggcaccgttcactt > 1:97106/1‑224 (MQ=255) tatCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCCCGCTCTTCGCTGTTTACTTGTTTTTTGAACCTGTCCCTTg > 2:248314/1‑239 (MQ=255) tAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTTGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTACCTTGAAAgg > 2:637698/1‑239 (MQ=255) tAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCATCTTCCCACACTGCATCAAGCGCCTGCCGGGGGGCATCGCGCTCTTCGCGGTTTCCTTGTTTGTTGGTACTGTCACTTGCAAgg > 2:121509/1‑239 (MQ=255) cTGAATCATTTCCCGCCTCCCGACCTGAGGTCGACATTTTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcag < 2:711108/239‑1 (MQ=255) tGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCAAACTGCATCAATCGCCTGGCGGGTGTCATCGCGCACTTCACTGTTTACTTGTTTTTTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTGgcccc > 2:526802/1‑236 (MQ=255) cgAATCTTTTCCCCCCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGGGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTG‑TTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca < 2:612538/238‑1 (MQ=255) gAACCATTTCCCCCCCCCCGCCCTGATGCCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCTCTGTTATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTgcagca < 2:756382/239‑1 (MQ=255) aaTCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag > 2:53205/1‑239 (MQ=255) aaTCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTAACTTGAAAGGGAGCTTCCCGCCAGTTGcagcag > 2:167487/1‑239 (MQ=255) tCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGGGCTTCCCGCCAGTTGcagcagg > 2:217432/1‑237 (MQ=255) ccttttCCCCCCCCCCGCTCTGTTTTCTATATTTTGCGGCCTACCATCCTGGCTAACGGGTGTGTGATATTGATTAAGCGGCGAGCTGTCCACCGCCGGGGCTTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCaga < 1:297144/235‑1 (MQ=255) aTTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACCCCGCACCTTGAAAGGGGACTTCCCCCCCGtttccacaaggat > 1:711108/1‑228 (MQ=255) tttCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGACCCTTTCACTTGAAAGGGAGCTTCCCGCCAGTTgcaccaaaacac > 1:144688/1‑233 (MQ=255) tgtgttcgaaattgtgcggccgtaCATCTTGGCTAAAGCGTGCATGATTTGGTTTAAGGCGCGTGTCGCCACACGCCGTGGCTTTATCTTCAATAAA‑CTTCC‑CCTTTTCCTCTTCCCCCACTGCATCAAGCGCCTGGGGGGTGTCATCGCGCTCTTCGCCGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCAc < 1:433053/216‑1 (MQ=255) aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTTTGCCACTGTCACTTGAAAGAGAGCTTCCCGCCAGTTGCAGCAGACAGTCAAACACAGCGTACCACgg > 2:97498/1‑239 (MQ=255) aTGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTTACCCTTTCCCTTGCAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTCCCACgg > 2:433053/1‑239 (MQ=255) gTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTTCAGCAGAGCATCAAACAACGCGTACCACGGt > 2:310036/1‑238 (MQ=255) atgtgggccCGAAATCCGGGCTAAAGCGTGGCTGATTTTGATTAGGCGGCCAGCTTTCCCAGTCCGGGGCGTTATCTTCAATAAA‑CTTCC‑CGTGTTCCTCTACCCTCCCGTCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt < 1:637698/234‑1 (MQ=255) atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgt < 2:610299/239‑1 (MQ=255) atTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGGGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGTGGCCTCGCgt > 2:493599/1‑239 (MQ=255) tGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCCGTTGCAGCAGAGCATCAAACAAAGCGTACCACGGGGCCTCGCgttg > 2:44138/1‑239 (MQ=255) cGCTGATATTGCTTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCCCTCTTAGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTGCCCGCCAGTTGCACCAGAGCAGCAAAAAAACCGTCACAAGGGGCCCCCCGGTGTTTTAAGCGCCCGCCAAAAAGGGCggg > 1:403962/1‑237 (MQ=255) cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGCAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACCCGTAACACGGTGCCTCGCGTTGTTTTAAGCGACCCCCAACCAGGGCggg > 1:725150/1‑237 (MQ=255) cGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGGGCCTCGCGTTGTTTTAAGGGACGGCCAAGCAGGGCGAg > 2:203447/1‑239 (MQ=255) gATTTTGATTAAGCGGCGAGCTGTCCCGCGCCGGGGCGTTAACCTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGAt < 2:433688/238‑1 (MQ=255) atTGATTAAGCGGCGAGCTGTCCCACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTCCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgc < 2:97106/239‑1 (MQ=255) ttGATTAAGCGGCGAGCTGTCACACGCCGTGGCTTTATCTTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACCCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg < 2:725150/239‑1 (MQ=255) gtgtttAACCGGGGTGGTGTCCCACGCCGTGGGGTTATTTTCAATAAA‑CTTCC‑CCTGTTCCGCTTCCCACCCTGCTTCAAGCGCCTGGGGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGGGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg < 1:53205/235‑1 (MQ=255) aGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAAAGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGCCGCAACCtt > 2:514087/1‑239 (MQ=255) ggcgccTGTCCCACCCCGTGGGGTT‑TCCTCAAAAAACCTTCA‑CCTGTTCCCTTTCCCCCACTGCAGCAAGCGCAGGGCGGGTGTCATCGCGGTCTTCGTTGTTTTCTTGTTTTGTGACACTGTCGCTTGAAAGGGAGCGTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc < 1:805193/234‑1 (MQ=255) cgggCTGCCCCACGACGTGGCGTTATCTTAAAAAAA‑CTTCC‑CCTTTTCCTCTCCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACCCTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc < 2:138537/235‑1 (MQ=255) cgAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCCGTTGCAGCAGAGCATCAAACAACGCGTCCCACGGGGCCTCGCGTTGTTTTAAGCGACCGCCAACAAGGGCGGGGGTCGGCGGAAAATTGTGCa > 1:745577/1‑239 (MQ=255) gAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACAGTGCCTCGCGTTGTTTTAAGCGACCGCCACGCAGGGCGAGGGGCGGCGCAAAATGGGGCaa > 1:479343/1‑239 (MQ=255) gAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACCCGTACCACGGTGCCTCGCCTTGTTTTAACCGACCGCCAAACCGGGCGAAGAACGGCGCAAAATTGAGCa > 1:351182/1‑238 (MQ=255) cggtgtgTTTTCTGCAATAAC‑CTCCC‑CCTGTCCCTCTTCCTCCACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTTTGTTGTGACACTGTCCCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTGCCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt < 1:273419/233‑1 (MQ=255) cGTGGCTTTTTCTTGAA‑ATA‑CTTTACCCTGCTCCTCTTCCCCCCCTGCATCAAGCCACTGAGGGGTGCGCTGGGTCTTTTCGCGGTTTACTTGTTTTGTGACACTGACACTTGAAAGGGAGTGTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt < 1:741210/239‑1 (MQ=255) cGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCCGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCg > 2:464219/1‑236 (MQ=255) cGTGGCGTTATCCTCAATAAA‑CTTCC‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTTTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt < 2:21462/239‑1 (MQ=255) ttgCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTAATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGGGAGGATCGGGGCAACATTGAGCAATCCTTCTTTCCCCTgg > 1:756366/3‑238 (MQ=255) tGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTTCAGCAGAGCATCAAACAACGCGTCCCACGGTTCCTCGCGTTGTTTTAAGCGACCGCCCAACAGGGCTGGGATCGGCGCAACCTTGGGCAATCCTTTTTTcccccac > 1:806246/1‑236 (MQ=255) tGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCCCGTTGTTTTAAGCGACCGCCAAGCAGGGCGGGGATCGGGGCAACATTTGGCAAACCTTCTTTTGCCTg > 1:378232/1‑238 (MQ=255) tCTTCAATAAA‑CTTAC‑CCTGTTCCTCTTCCCACACTTCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCTGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAgg < 2:585875/239‑1 (MQ=255) ttCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGAATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGGCGGGATCGGCCCAACATTGAGCAATCCTTCTTTCGCCTGATCggcaggca > 1:115121/1‑239 (MQ=255) aaTAAC‑CTTCC‑CCTGTTCCCTTTCCCACCCTGCATCAAGGGCCTGGCGGGTGGCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTa < 1:479776/239‑1 (MQ=255) aaTAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGCGCTTCCCGCCAGTTGCAGCAGAGCATCACACAACGCGTACCACGGTGCCTCGCGTTGTTTTAATCGACCGTCAAGCAGGGAGAGGCTCGGCGCAACATTGAGCAATCCTCCTTCCGCCTGATCGCCCGGCAgca > 1:602283/1‑237 (MQ=255) aaTAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTTAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGt > 2:54737/1‑238 (MQ=255) a‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCCCGTTGTTTTAAGCGCCCGCCAAGCAGGGCGAGGATCGGCGCAACATTGGGCAATCCTTCTTTCGCCTTATCGTCAGGCAGGACGCtt > 1:387922/1‑239 (MQ=255) tCA‑CCTTTTCCCCTTCCCACACTGAATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa < 2:144688/239‑1 (MQ=255) cA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAAt > 2:479776/1‑239 (MQ=255) cA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGAACGCTTaaaa > 1:494366/1‑238 (MQ=255) a‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCCACATTGAGCAATCCTTCTTTCCCCTGGTCGTCAGGCAGTAAGCTTaaaaa > 1:746035/1‑237 (MQ=255) | GAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCCACCTCCCGCACTGATGTCGACATATTGCGGCGCGACATCCTGGCTAAAGCGTCGCTGATATTGATTAAGCGGCGAGCTGTCACACGCCGTGGCGTTATCTTCAATAAA‑CTTCA‑CCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATA > NC_000913/1536499‑1536973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |