| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,151,689 | G→T | 53.1% | intergenic (+43/+22) | yegJ → / ← yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,151,689 | 0 | G | T | 53.1% | ‑5.1 / 3.8 | 13 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (5/2); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01 | |||||||||||
ACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGC‑GCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGC > NC_000913/2151486‑2151926 | atgtaaaatggggCAAGGAGTGAAAAAACAAAAAAGAAAAT‑TTTCAGGCTGGTGGTTAACACGGGGGGGAAAAATTTTGCGGGGT‑TTTAACCTTTAATCTTTTATTGGTCCCCTTTCAAAAAAAAGAAGCCGCCGAGCAAAGAGAAAAATTAGTTCGCTAATCAGCGTTTATTTTTATTGTTAAAACG‑GGCGTTTAAAT‑TGTTGTCTT‑AAACCGCCATGAGAAACTCACTCTGCCcaca < 2:184374/228‑1 (MQ=255) agaggtgtAAAAAAAAAAAAAAAAATTTTTTAGGCTGTATTTTTTCGC‑GGGGGGAAAAAATTTGCCGTGGTTTATACCTTTGATATTTTGGTGTTCACCAACAAAAAAGAATAACCCGAGGAGCAAAGGAAAAAATTGTTGCTCTAA‑CAACCGTTTTTTTTTTTTTTTAAAGTGGGGGGTTGATT‑GTTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa < 1:693269/234‑1 (MQ=255) ttGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTTTCTT‑CAACCGCCCTTGGAAAATCACCCTTGCCCCAGCGCCCAGGCCCACGGTTTTTACACATCCCTACCCCCGGTTT‑CCGCCCGGCTGGGCAAAAACATCTTTAACCATTTAGGGaaaaaaatcatgt > 2:614384/1‑232 (MQ=255) tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TGTTGTCTT‑TCACCGCCATGAGAAAACCACTCTTTCCACAGCGCCACTGCCCAAGCTTTATCATCTTCCGTACGCTCCCTTA‑CCTCCCGGGCGGGCACAAATACATTTAACAATTCAGGTAACAAATCgc > 2:827086/1‑234 (MQ=255) aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGTGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGGAT‑TGTTTTCTT‑TAAACGCCATGGGAAACTCACTCTTCCCACAGCGCCAGTTCCAACGTCTTATCATTATTCTGACCCCCGTTTT‑TCGCCGGGGTGTGCAAAAACAGCTTTTACAATTCAGGCAATAAAtatcctttg > 2:832377/1‑230 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTGAA‑TGTTGTCTT‑CAACCCCCCTGAGAAACTCACTCTTCCCCCAACGCCAGTGCCAACAGTTTTTTATCATCCGTACACCCGTTTACCCCCCGGGTGGGACAAAAACTCTTTTAACAATTCGGGAaaaaaaaacgagtttcccgc > 1:226889/1‑225 (MQ=255) aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTTTCTT‑CAACCGCCATTAGAAAATCACTCTGCCCCCAGCGCCCGTGCCAACGGTTTATTCTCATCCCTACGCCCGTTTA‑CCGCCCGGCCGGGCAAAAACTGGTTTTACAATTCAGGTAATAAAACGAGGTGTTTCTCGGTtgctgc > 2:718140/1‑238 (MQ=255) ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TTTTTTCTT‑CAACCGCCATTAGGAAATCACTCTGCCCCCAACGCCAGTGCCCAAACTTTATCATCATCCCTCCGCCCGCTTT‑TCGCCACGGCGGCAAAAAAATACTTTTACAAATACTGGAAAAAAACGAGATGTTCATACGTTGTTgccg > 1:130752/1‑239 (MQ=255) cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCCTTAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGGTTA‑CCGCCGGGCTGGGCAAAAACTGCTTTTACAATTTAGGTAATAAATAGAGTTGTTTCTGCGGTTCTgccg > 2:135487/1‑238 (MQ=255) tgttcgcaaaatcgcctttttttttattTTTTAAAGT‑GGGGGTTTAAT‑TGTTTTTTTAAAACCCCCATGAAAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag < 2:778136/217‑1 (MQ=255) tCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TGTTTTCTT‑CAACCTCCCTGAGAAACACACACTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACCCCCGCTTA‑CCTCCGGGCTGGCCAAAAAATTCTTTAACAAATCACGTAATAAAAAGCGTTGTTCCTGCGTTTCTGCGCCCAGTCCCTTGAAAAAAGGGGGaaaaaaaaaaaaaaagggaga > 1:856347/1‑225 (MQ=255) cTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TTTTGTCTT‑CCACCGCCATGAGAAAATCACTCTTCCCACAGCCCCCAGGCCAAACTTTTTTCATCATCCGCAAGCTCGTTTT‑CCGCCCGGCTGGCCAAAAAATGCTTTTAAAAATCACGTAATAAAACCGGTTGTTCCTGCGTTGGTGCCCCCCGCCCATTGAAAAAAGGGGGAAAAAAAGGAACAATAGGGGaaaa > 1:778136/1‑237 (MQ=255) tgttAAACT‑GGGCGTTTATTGTGTTTTTTG‑CAACCGGCATGGGAAACTCACTCTGCCCACAGCCTCAGTTCCAACGTTTTATCCACATCCGTACGCTTGGTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 2:856347/237‑1 (MQ=255) ggtttaattttttgtGTC‑CACCCGCCATGGAAAACTCCCTCGGCCCCCAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc < 2:596470/227‑1 (MQ=255) gtCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGCGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGTTACCATCCGTAAAtgc > 2:413218/1‑238 (MQ=255) | ACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGC‑GCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGC > NC_000913/2151486‑2151926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |