Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,154,491 T→C 20.2% L159P (CTC→CCC)  mdtA → multidrug efflux system, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,154,4910TC20.2% 121.5 / 4.3 72L159P (CTC→CCC) mdtAmultidrug efflux system, subunit A
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base C (9/7);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
aTTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGttt                                                                                                                                                                                                                                     >  2:330767/1‑238 (MQ=255)
         gcTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTACCCAAAGCCCAAGGCCACCTGGCACAAGATAAAGCCACGCTTGACAACGCCCGCCGGGACCTGGCGCCGTATCAAACACCGGCAAAAACCAATCCCGTTTCCCGCCCGGa                                                                                                                                                                                                                           >  1:237821/1‑239 (MQ=255)
                   ttACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCCCGCTTGCCCACGCCCGCCGTGACCTGGCGCGTTATCAACAAATGGCAAAAACCACACCCGTTTCCCGCCGGGAGCTGGATGc                                                                                                                                                                                                                  >  1:689588/1‑238 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTTTCAACAACTGGCAAAAACCAATACCGTTTCCCCCCAGGAGCTGGATGCCCAAAAGgc                                                                                                                                                                                                         >  1:292347/1‑239 (MQ=255)
                           cgcAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGgc                                                                                                                                                                                                         >  2:314236/1‑239 (MQ=255)
                             cAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCAAAAGCCCAGGGCCAACGGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGCCCTGGCGCGTTATGAACACACGGAACAACACAACCCCGGTCCCCGCCAGGAGCCGGAGGCCCAACaagggc                                                                                                                                                                                                       >  1:563080/1‑234 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACACCTGGCAAAAACCAACCCCCTTTCCCGCCCGGGGCGGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  1:716453/1‑239 (MQ=255)
                                ccGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTgg                                                                                                                                                                                                    >  2:690556/1‑239 (MQ=255)
                                     tGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGTAGCCCAACAGGCGCTGGTCAGt                                                                                                                                                                                               >  2:247081/1‑239 (MQ=255)
                                        aCGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGCTAAAGCCACGCTTGCCAACGCCCGCCCTGACCTGGCGCGTTATCAAAAACTGGCAAAAACCAATCCCGTTTCCCGCCCGGGGCTGGATACCCAAAAggacatggacaaggaa                                                                                                                                                                                            >  1:858767/1‑225 (MQ=255)
                                                 gaatggttttatttttCCAGGAAGGCCGGCAGGAAAAGGAAGGTGTTTTACTGGAAAAAATTGCCCCAAGCCAGTTAAAAGTTGCATGAGCACAAGCCCGGGGCCACCGGGCAAAAGATAAACCCCCGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGc                                                                                                                                                                                  <  1:314236/226‑1 (MQ=255)
                                                      gCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCATGCGCGTTATCAACAACTGGCAAAACACAATCTCGTTTCCCCCCCGGACATGGGTGCCCAACGGGCCGTGCTCACTGAAAcacacgggcacaa                                                                                                                                                                             >  1:121728/1‑227 (MQ=255)
                                                      gCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAACCCACGCTTGCCAACGCCCACCGTGCCCTGGCGCGTTATCAACAACTTGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACACGCGCTGGACAGAGAACCCGAAGGCAcc                                                                                                                                                                               >  2:159241/1‑237 (MQ=255)
                                                          tACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCATTGAAACCGAAGGCAACATTAAg                                                                                                                                                                         >  2:184469/1‑239 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAg                                                                                                                                                                         >  2:863597/1‑238 (MQ=255)
                                                           aCATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCaa                                                                                                                                                                                                              >  2:757148/1‑202 (MQ=255)
                                                                 ccgagAAGGCCAGAGGGACAAAGCAGGCGTTTTACTGGCAAAAATTGCCCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCAAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCtgatg                                                                                                                                                                  <  2:226057/235‑1 (MQ=255)
                                                                 ccAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCCAAAACCAATCTCGTTTCCCGCCAGGAACTGGATGCCCAACAGCCGCTGGGCAGTGAACCCGAAGGCACCCTTTATGCtgaag                                                                                                                                                                  >  2:801966/1‑237 (MQ=255)
                                                                      aaGGCCGGCAGGTCAAGGCAGGCGTTTACCGGGAAAAATTGGACCCCAGCCAGTCCAAAGTTCAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAAAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaag                                                                                                                                                               <  2:428537/237‑1 (MQ=255)
                                                                       gggCCAGGAGGTAAAAGCAGTCGTTTTACGGAGAGAATGGCACCCACCCCTGTTCAAAGTTGCATTAGCCAAACCCCGGGGCCACCGGGCAAAAGAAAAACCCACGTTTGCCACCGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  1:516880/237‑1 (MQ=255)
                                                                       aGGCCAGCAGGTCAAAGCGGGGGATTACCGGGCAAAAATTGCCCCCAGCCAGTTAAAAGTTGAATTAGCACAAGCCCAGGGCCAACGGGCAAAAGAAAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGaagca                                                                                                                                                             <  2:716453/238‑1 (MQ=255)
                                                                        ggCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCCCTGGTCAGTGAAACCGAAGGCCCCATTAAAGCTGATGaagaaag                                                                                                                                                           >  1:193844/1‑239 (MQ=255)
                                                                         gCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCACGGTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGGGAAACCCAAGGCACCATTAAgagtgagaaagaaaac                                                                                                                                                          >  1:366414/1‑224 (MQ=255)
                                                                                   cAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAATCCACGCTTGCCAACGCCCGCCGTCACCTGGCGCGTTATCAACCACTGGCAAAAACCAATCTCGGTTCCCGCCAGTGGCCGGATGCCCACAAGCCCCTGGCCCGGGAAACACCAGACACCATTAAGGACAAAGaagcaagagattcaaagg                                                                                                                                                >  1:388806/1‑228 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGCAGGAACCATTAAGGCTGATGAAGCAACCGTTTCCTGCGCGCAGcct                                                                                                                                        >  1:158874/1‑237 (MQ=255)
                                                                                           gCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCCGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCCAAGGCCCACTTAGGGCTGCTGAAGCCACCGCTGCCAGGCCGCAGCt                                                                                                                                         >  1:55720/1‑238 (MQ=255)
                                                                                                                        cacgTTGAAATTTGCATTGGCAAAAGCCAGGGGCCAACGGGAAAAAGATAAACCCAAGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCAc                                                                                                            <  2:767962/236‑1 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACa                                                                                                                                                                                                            >  2:330064/1‑130 (MQ=255)
                                                                                                                                      cATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGCCTGGAGCCGGATTACCGCACCAGCCGAGGGTcgc                                                                                              >  2:391665/1‑239 (MQ=255)
                                                                                                                                        ttACCACCACCCCGGGCCCATCTGGAAAAAAAAAACCCCACCTTTGCCAACCCCCGCCGTGCCCTGGCGCGTTACCAACAACGGGCAAAACCCAATCTCGGTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGt                                                                                            <  1:242013/239‑1 (MQ=255)
                                                                                                                                        tgagcccaggccaggcgCCACCTGGAAAAAGATAAAGCCACGCTTGCCAACCCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGt                                                                                            <  2:292347/223‑1 (MQ=255)
                                                                                                                                           gCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGACGAAGCAAGCGTTGCCAGCCCGAAGCTCCAACTCGACCGGAGCCGGCTTACCGCACCAGCCCATggctgccttg                                                                                          >  2:425021/1‑230 (MQ=255)
                                                                                                                                             ccaACCCCCGGGCCCATGGGGAAAAGATAAAACCCCCGCTTCCCACCCCCCGCCGTGACCGGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGt                                                                                        <  1:690556/238‑1 (MQ=255)
                                                                                                                                                aGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGACGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGGCGCACCCCAACGGGAGCCGGATTACCGCCCCAGCCGATGGTCGCGTTgggccaa                                                                                    >  1:499818/1‑234 (MQ=255)
                                                                                                                                                 gCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGGCAGCGCGCAGCTGCAACTCGACTGGGTCCGGATTAACGGCCCAGCCGACggggcggttgggcccaa                                                                                   >  1:73127/1‑224 (MQ=255)
                                                                                                                                                   ccAGGCCAAACTGGAAAAAAAAAAAGCCACGGTTGCCAAGCCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAg                                                                                  <  2:700888/238‑1 (MQ=255)
                                                                                                                                                      gggCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAgg                                                                              >  2:169768/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGTGCTCAAGCAGGTTGATg                                                                        >  2:752410/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATg                                                                        >  2:231589/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCAGCAACTCGACTGGAGCCGGATAACCGCACCAGCCGATGGTCGCGTTGGGCTCAAGCAGGTTGATg                                                                        >  2:880907/1‑239 (MQ=255)
                                                                                                                                                            aCTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGCTGCCAGCGCGCCGCTGCAACCCGACTGGGGCCGGGTTACCGCCCCAGGCGATGGGCCCGTGGGGCTCACGCCGGTTGagt                                                                        >  1:66664/1‑237 (MQ=255)
                                                                                                                                                                        taaaaCCCACTTTTGCAAACGCCCGCCGTGACCTGGCGCTTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  2:657492/237‑1 (MQ=255)
                                                                                                                                                                        ctacccccccttttccccccccccgccGGGACCGGGCGGTTTAACAAAAACGGGAAAAAACCAATCTCGTTCCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  1:169768/218‑1 (MQ=255)
                                                                                                                                                                        ataaaccccccttgcccAACGCCCGCCGTGACCTGGCGCGTTACCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  2:158874/224‑1 (MQ=255)
                                                                                                                                                                        acaaccgcctctttGCCACCGCCCGCCGCGACCTGGGGCTTTAGCAACACTGGGCAAACACCAATCTCTGTCCACGCCAGGAGTGGGATGCCCAACAGGCGCTTGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAAATCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCaa                                                             <  1:656662/227‑1 (MQ=255)
                                                                                                                                                                           aaGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAGTCAGGTTGATGTTGGTAACCAAAt                                                           >  2:252960/1‑238 (MQ=255)
                                                                                                                                                                                  gCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGACGGTGGTAACCAAAACTCCAatg                                                   >  2:185925/1‑239 (MQ=255)
                                                                                                                                                                                      gCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGACCCGGATTACCGCACCAGTCGACGGCCGCGTCGGTCCCAAGCAGGTTGATGTTGGTAACCAAATCACCAGGGGGGa                                               >  1:700823/1‑239 (MQ=255)
                                                                                                                                                                                       ccccccccccccgggcacTGGAGCGTTACCAACAATGGGCAAAAACCAACCTCGGTTCCCGCCAGGGGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCAGTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGAt                                              <  1:250878/222‑1 (MQ=255)
                                                                                                                                                                                       ccactgcccgccGTGACCTTGGGGTTTAACAAAAACCGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCTCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGAt                                              <  1:180080/234‑1 (MQ=255)
                                                                                                                                                                                        cAACGCCCGCCGGGACCTGGCGGGTTAGCAACAACGGGAAAAAACCAATCGCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATa                                             <  2:827476/239‑1 (MQ=255)
                                                                                                                                                                                         cccccccgccGTGTCCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATac                                            <  1:391665/235‑1 (MQ=255)
                                                                                                                                                                                             ccccccTTGCCCTGGCGCTTTTTTAACAACTGGAAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGACCAACAGGCGCTGGTCAGTGAAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATaccac                                         <  1:330767/239‑1 (MQ=255)
                                                                                                                                                                                                    tgcccTGGCGCGTTATCAACAACCGGCAAAAACCATTCTCGTTTCCCGACAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCg                                 <  2:402075/236‑1 (MQ=255)
                                                                                                                                                                                                     gcccTGGAGCTTTATCCACAACTGGCAAAAACCAAACTCGTTGCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGAAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  2:699507/237‑1 (MQ=255)
                                                                                                                                                                                                      aCCGGGCGCGTTAACAAAAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgt                                <  2:699506/238‑1 (MQ=255)
                                                                                                                                                                                                        cgggCGCGGTATAAACAACTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggt                             <  2:143650/237‑1 (MQ=255)
                                                                                                                                                                                                         tGGGGCGTTACCAACACCTGGCAAAACCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  2:182002/239‑1 (MQ=255)
                                                                                                                                                                                                         tGGCGGGTTACCAACAACTGGCAAAAGCCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCgtggtg                            <  2:138913/239‑1 (MQ=255)
                                                                                                                                                                                                          ggggcgTTATCAACAACTGGCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGa                           <  2:22090/236‑1 (MQ=255)
                                                                                                                                                                                                          ggCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAACCGTTGCCAGCGCGCAGCTGCACCTCGCCTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCCCAAGCAGGTTGATGTTGGTAACCAAAACTCAAGTGGTGATAAAACCGGGATCggggtgc                           >  1:574553/1‑238 (MQ=255)
                                                                                                                                                                                                            cgcgTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGAt                          <  2:66664/238‑1 (MQ=255)
                                                                                                                                                                                                               gTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  2:168757/239‑1 (MQ=255)
                                                                                                                                                                                                                ttaacaaacacTGGAAAAACCCAAGCTCTTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCAcc                      <  1:247081/229‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGGAAAAACCAATCCGGTTTCCCGCCAGGAGATGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCATCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATTGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCTcc                      <  1:159241/238‑1 (MQ=255)
                                                                                                                                                                                                                ttATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGTTCGTGGGGATCAccc                     >  2:207520/1‑239 (MQ=255)
                                                                                                                                                                                                                         acgtgaaaaaaCCAATCTCTTTTCCCCCCAGGGGCTGGATGCCCAACAGGGGCTGGTCGGGGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATc            <  1:8451/233‑1 (MQ=255)
                                                                                                                                                                                                                            ggCAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACGGGCGCTGGTCAGTGAAACCGAAGGCCCCCTTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTa         <  2:121728/239‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAACCCTATCCCGTTTCCCGCCAGGGCGTGGACGCCACACAGGCGCTGGTTAGTGAAACCGGAGGCACCATTAAGGCTGAAGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  1:131415/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  2:689588/238‑1 (MQ=255)
                                                                                                                                                                                                                              cAAAAACCAATCCCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTAt        <  2:578393/238‑1 (MQ=255)
                                                                                                                                                                                                                                   aCCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGAttt  <  2:574588/239‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
ATTACCGCCGCTAATACCGTTACGGTGCGCAGCCGCGTGGACGGCCAACTGATAGCGTTACATTTCCAGGAAGGCCAGCAGGTCAAAGCAGGCGATTTACTGGCAGAAATTGACCCCAGCCAGTTCAAAGTTGCATTAGCACAAGCCCAGGGCCAACTGGCAAAAGATAAAGCCACGCTTGCCAACGCCCGCCGTGACCTGGCGCGTTATCAACAACTGGCAAAAACCAATCTCGTTTCCCGCCAGGAGCTGGATGCCCAACAGGCGCTGGTCAGTGAAACCGAAGGCACCATTAAGGCTGATGAAGCAAGCGTTGCCAGCGCGCAGCTGCAACTCGACTGGAGCCGGATTACCGCACCAGTCGATGGTCGCGTTGGTCTCAAGCAGGTTGATGTTGGTAACCAAATCTCCAGTGGTGATACCACCGGGATCGTGGTGATCACCCAGACGCATCCTATCGATTT  >  NC_000913/2154259‑2154722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: