Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,507,675 T→C 12.4% E73G (GAG→GGG)  fryC ← putative enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,507,6750TC12.4% 191.7 / 2.1 77E73G (GAG→GGG) fryCputative enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (32/33);  new base C (5/6);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCGA  >  NC_000913/2507442‑2507910
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTCTTAAGAGCATCCATGAAGCCAATCAcacca                                                                                                                                                                                                                                        >  1:261909/1‑239 (MQ=255)
tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGACCTcagca                                                                                                                                                                                                                                        >  2:211146/1‑239 (MQ=255)
  cTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGCAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGAGGCCGATCTcacaagg                                                                                                                                                                                                                                      >  2:299335/1‑234 (MQ=255)
   tGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATACCGAGCTCAGCAGGa                                                                                                                                                                                                                                     >  1:23418/1‑239 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGGCTGCGACAGCCAGGCAGATTTAAGTAAGGAGGGGTCGAAACCGGAGACTTTCCCGCTATTAAAGGCATCCCTGATGCCGACACCAGCAGGa                                                                                                                                                                                                                                     >  2:492924/1‑237 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGGGAGGTCGAAACCGGGAAATTTCCCGCTATTAAGAGCATCCATGAGGCCGAACTCAGCAGGAAt                                                                                                                                                                                                                                   >  1:219870/1‑239 (MQ=255)
     ggATCGAAGTTCAGCAGTTGCGTCGGCTGGGAAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAACTTAAGTAAGGAAAGGTGGAAACCGACGAATTTCCCGCTATTAAGATCATCCATGATGCCGATCCCAGACGGAAt                                                                                                                                                                                                                                   >  2:162969/1‑239 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAGACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCCCCATTAAGAGCATCCATGATGCCGATCCCGGCAGGAATTTTa                                                                                                                                                                                                                               >  2:162027/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACACAACGCCCCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGACAGGCCGAAAACGGCAAATTTCCCGCTCTTAAAAACCACCAAGATGGCGATCCCCCCAAGAATTTTa                                                                                                                                                                                                                               >  1:317340/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCTCCGAAGGGTTGCGACAGCCAGGCAAATTTAAGTACGGAGAGCGCGAAACCCGAGCATTTCCCGCTATTAAAAGCCTCCCTGCTTCCGCTCTCAGCAGGGAttttt                                                                                                                                                                                                                               >  1:67676/1‑237 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTa                                                                                                                                                                                                                               >  2:715285/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCCCAGCAGGAATTTTa                                                                                                                                                                                                                               >  2:681009/1‑238 (MQ=255)
          gAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAAAGCATCCATGATTCCGATCTAAGCAGGAATTTTaa                                                                                                                                                                                                                              >  2:823414/1‑239 (MQ=255)
                       ggggggggggggaaaacaacaaaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGc                                                                                                                                                                                                                 <  2:298666/218‑1 (MQ=255)
                           tCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCAATAATGCCGATCTCAGCAGGAATTTTAAGCCAACAATaaaaaaaa                                                                                                                                                                                                             >  1:585500/1‑233 (MQ=255)
                                tggtaacccccaaccccGCCGAAAAAACCAGCCGGGAGCGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACGCGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAgct                                                                                                                                                                                                         <  1:227983/224‑1 (MQ=255)
                                tGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAAAAACACGCCGCCGAAGGACTGCGACAGCCCGGCAAATTTAAGTAAGGAGAGGACGCAACCGGAGAATTTACCGCTATTAAGAGCATCCCTGATGCCGATCTCAGCAGGGATTTTAAGCCCGCAATAAGCAAACAgctg                                                                                                                                                                                                        >  1:528265/1‑239 (MQ=255)
                                       cccccccccccccGAAAAACCCAGCCGGGAACCCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGaaa                                                                                                                                                                                                 <  1:756020/232‑1 (MQ=255)
                                         cacccccccccGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  1:211146/234‑1 (MQ=255)
                                         accAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  2:105935/237‑1 (MQ=255)
                                         aaccccccGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  2:351618/234‑1 (MQ=255)
                                         aTCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               >  2:53835/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGGTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCACCTGGCTGAAAACGAg                                                                                                                                                                                            >  2:881497/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAg                                                                                                                                                                                            >  2:302706/1‑239 (MQ=255)
                                            aaCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTAAAAGCAATAAACGGGCTGAAAGCGAg                                                                                                                                                                                            >  1:278969/1‑239 (MQ=255)
                                             ccccGCCGAAAAAACCAGCCGGGACCGCCAGTTTGCCCCCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAGACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGc                                                                                                                                                                                               <  1:162027/234‑1 (MQ=255)
                                                   caaaaaacCCAGCCGGGAACCCCCGTTTGCCCCCGAAGGAGTTCGCCCCAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATcaca                                                                                                                                                                                     <  1:823414/235‑1 (MQ=255)
                                                        aaaCCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGcc                                                                                                                                                                                <  2:627704/239‑1 (MQ=255)
                                                             aGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATaa                                                                                                                                                                           >  2:296681/1‑239 (MQ=255)
                                                               ccctgAACCCCAGTTTGCCACGGATGAGGTTCGCCACAAAACCAGAACACGTCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACCGGCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTCCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACCCCGCCCATAAtt                                                                                                                                                                         <  1:753031/235‑1 (MQ=255)
                                                               cccggAACCCCAGTTTGCCACCGATAGAGTTCGCCCCAAAAGCAGCAAACAGCGGAATGGAAAAACCAAACAACCCGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         <  1:53835/236‑1 (MQ=255)
                                                               ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         <  2:219870/239‑1 (MQ=255)
                                                               ccGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAAtt                                                                                                                                                                         >  2:39570/1‑239 (MQ=255)
                                                                 ggaaccGCCCGTTTGCCACCGATAAAGGTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGGAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTaa                                                                                                                                                                       <  1:704998/234‑1 (MQ=255)
                                                                         cAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGACGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCAAGCTATAAGAAATCAGCTGGCTGAAAGCAAGGATCCAACCCACACTAATTAAGGTCGGc                                                                                                                                                                >  1:383444/1‑238 (MQ=255)
                                                                                ccACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACCCGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCg                                                                                                                                                        <  2:334649/239‑1 (MQ=255)
                                                                                  cccGACAGAGTTGCCACCAAAAGCACCAAACATCTGAATGGGAAAACCAAACAACACGCCGTCGAAGGGATGCGACAGCGAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTg                                                                                                                                                      <  1:259306/238‑1 (MQ=255)
                                                                                             tCGCCAAAAAAGCAGCAAACAGCGGAATGGAAAAACCAAACAACCGGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTAATAAGAGAATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGAc                                                                                                                                           <  2:67676/239‑1 (MQ=255)
                                                                                              cGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGCTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCCCCCCTAATTAAGGTCGGCACCATGCGTGAAATCCCTGAc                                                                                                                                           >  1:474302/1‑238 (MQ=255)
                                                                                                    aaaaaGGAGAAAACAACGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGAGGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATcaca                                                                                                                                     <  1:539261/238‑1 (MQ=255)
                                                                                                            gcaAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATTCGTGAAATCCCTGACATCACATGCTCCgg                                                                                                                             >  2:594057/1‑239 (MQ=255)
                                                                                                              aaaCATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACAtgctgc                                                                                                                               <  2:261909/235‑1 (MQ=255)
                                                                                                                 cATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGtt                                                                                                                        <  2:420273/239‑1 (MQ=255)
                                                                                                                  cgcgggttggacaaaCCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATGAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTc                                                                                                                       <  1:107431/227‑1 (MQ=255)
                                                                                                                     ggAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTACGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCAAAATTAAGTCCGGCCCCAGACGTGAAATCCCTGACAtacaagactgaggggggtaac                                                                                                                     >  1:156102/1‑218 (MQ=255)
                                                                                                                                         cacGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTagag                                                                                                 >  2:117510/1‑238 (MQ=255)
                                                                                                                                              cggcgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCTGCTATTAAGAGCAACCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAAGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGc                                                                                           <  2:838112/236‑1 (MQ=255)
                                                                                                                                               gccgAAGGGCTGCGACAGCCAGGCAAATTTAAGTAAGGATAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCg                                                                                          <  2:309811/239‑1 (MQ=255)
                                                                                                                                                ccgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCAAGGATAACACCGCCCATAATTAAGATCGGCACCCTCCGGGAAACCCCTGACATCACATGCTGCGGGAGTTCCCCCCAGAACCTGGTTTTAGAGGGCTgggg                                                                                         >  1:233367/1‑239 (MQ=255)
                                                                                                                                                 cgAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGGCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGa                                                                                        <  2:11115/239‑1 (MQ=255)
                                                                                                                                                           cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAAct                                                                               >  2:713459/1‑238 (MQ=255)
                                                                                                                                                           cGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAAct                                                                               >  2:54727/1‑238 (MQ=255)
                                                                                                                                                               cgCCAGGTCAATTGAAGTAAGGAGAAGTCCAAACCGGAAAATTTCCCCTTTTTAAGGACAACCATTATGCCGGTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCCGCTGGCTGAAAGCAGGGATCACACCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAActgccg                                                                           <  1:57352/237‑1 (MQ=255)
                                                                                                                                                                gCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGCCATCACATGCTCCGGGCGTTCAACCCAGAAGCTGGTTTTAGAGGCCTGCGGGTTCTTAActgcg                                                                            >  1:709838/1‑235 (MQ=255)
                                                                                                                                                                               gtaagGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGACCTGCGGATTCTTAACTGCCGCTCCCGCACCgggagc                                                           >  1:447138/1‑234 (MQ=255)
                                                                                                                                                                                 aagggagggTCAAAACCGGAAAATTTCCCCCTATTAAGGGCATCCATGATGCCGATCCCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGcc                                                        <  1:715285/232‑1 (MQ=255)
                                                                                                                                                                                 aagGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGcc                                                        <  2:54248/239‑1 (MQ=255)
                                                                                                                                                                                        ggTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  2:278969/238‑1 (MQ=255)
                                                                                                                                                                                        ggTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCAGGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  2:84930/238‑1 (MQ=255)
                                                                                                                                                                                        agTCGAAACCGGAGAATTTCCCGCTATTAAGAGCAGCCATGAGGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGcac                                                  <  2:195116/237‑1 (MQ=255)
                                                                                                                                                                                         gTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACGGCCGCTGCCGCACCGGATCCGCCGGGCCCaa                                                >  2:680075/1‑239 (MQ=255)
                                                                                                                                                                                              aaCCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAGGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCCCTGCCCCACCCGagtgcccggggcaaaaggtt                                           >  1:345021/1‑220 (MQ=255)
                                                                                                                                                                                                 cGGAGAATTTCCCGGTATTAAGAGGATCCATGATGCCGATCTCAGCAGGAATTTGAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCa                                        <  2:632487/239‑1 (MQ=255)
                                                                                                                                                                                                   gagaATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCCCCCCAGAACATGGGTTTAGAGGCCTGCGGGTTCTTAACTGCCCCTGCCGCACCGGATGCGCCAGGCACAACGGGTGCAca                                      >  1:801656/1‑238 (MQ=255)
                                                                                                                                                                                                    agaATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACt                                      <  2:23418/238‑1 (MQ=255)
                                                                                                                                                                                                             cgttATTAAGAGCATCCATGAGGCCGATCCCAGGAGGAATTTTAAGCCAGCTAAAAGCAATCAGCTGGGTGAAAGCGAGGATCACACCGCACATAATTAAGGTCGGCCCCAGGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGGTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTa                            <  1:302706/236‑1 (MQ=255)
                                                                                                                                                                                                                     agagacaccATGATGCCGATCCCAGCAGGAATTTTAAGCCAGCTAAAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCAt                    <  2:596677/232‑1 (MQ=255)
                                                                                                                                                                                                                     agagCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCAt                    <  2:624845/239‑1 (MQ=255)
                                                                                                                                                                                                                        gcctCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATa                   <  2:183164/234‑1 (MQ=255)
                                                                                                                                                                                                                          aTCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCCCCAGGCACAACGGTTGCCCTGCGTTTTTCAATGGCCATAAcca               >  2:456433/1‑237 (MQ=255)
                                                                                                                                                                                                                          aTCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCCCCAGGCACAACGGTTGAACTGCGTTTTTTCATGGCCATAaaaa               >  2:379435/1‑239 (MQ=255)
                                                                                                                                                                                                                            ccATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGGTGCGCCAGGCACAACGGTTGCACTGGGTTTTTTAATGGCCATAaaaaaa             >  1:387222/1‑237 (MQ=255)
                                                                                                                                                                                                                                gtcgCCGAGCCCAGGAGGAATTTTAAGCACACTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGTCCATAATTAAGGTCGGCACCAGGCGTGAAACCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATcc         <  1:681009/236‑1 (MQ=255)
                                                                                                                                                                                                                                   gCCCATTTCAGCAGGAATTTTAAACCAGTTTTAAGCAAACAGCGGGCCGAAAGCGAGGATCACCCCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTg      <  1:296681/239‑1 (MQ=255)
                                                                                                                                                                                                                                     cGATCCAAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTg      <  2:796467/237‑1 (MQ=255)
                                                                                                                                                                                                                                      gATCCCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCCCCCCGCCCATAATTAAGGTCGGCCCCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCg   <  2:795627/239‑1 (MQ=255)
                                                                                                                                                                                                                                       aTCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCCGAAGCTGGTTTTACAGGCCTTCGGATTCTTAACTGCCCCTGCCGCCACGGATGCCCCCGGCCCCACGGTTGGCATGCGCTTTTTCAATGCCATCACAAATATCCCAGTcca  >  1:411647/1‑237 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                           
TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCAAAACCAAACAACACGCCGCCGAAGGACTGCGACAGCCAGGCAAATTTAAGTAAGGAGAGGTCGAAACCGGAGAATTTCCCGCTATTAAGAGCATCCATGATGCCGATCTCAGCAGGAATTTTAAGCCAGCTATAAGCAATCAGCTGGCTGAAAGCGAGGATCACACCGCCCATAATTAAGGTCGGCACCATGCGTGAAATCCCTGACATCACATGCTGCGGGAGTTCACCCCAGAAGCTGGTTTTAGAGGCCTGCGGATTCTTAACTGCCGCTGCCGCACCGGATGCGCCAGGCACAACGGTTGCACTGCGTTTTTTAATGGCCATAACAATTATCCCTGTCGA  >  NC_000913/2507442‑2507910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: