| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,590,063 | A→G | 16.8% | E824G (GAG→GGG) | acrD → | aminoglycoside/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,590,063 | 0 | A | G | 16.8% | 155.0 / 3.5 | 71 | E824G (GAG→GGG) | acrD | aminoglycoside/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base A (23/33); new base G (11/4); total (34/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTA > NC_000913/2589831‑2590289 | acacTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGAttgt > 2:643167/1‑239 (MQ=255) acTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGAttgttg > 2:54364/1‑239 (MQ=255) acTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGAttgttg > 2:697399/1‑239 (MQ=255) tGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTCTTCTGCGG‑TTGGGATTGTTggg > 2:764676/1‑239 (MQ=255) tGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGGCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGTTTGTTggg > 2:435318/1‑239 (MQ=255) gCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGGGCCCTTCTCTGCTTTCGCGACCTCCCGCTGGGGAACAGG‑CTGCCCGCGTCTGGAAGCCCATAACGGTTTTTTTGGGG‑TTGGGGTTTTTGGGGAAGccc > 1:736728/1‑237 (MQ=255) ccTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGc > 2:790878/1‑238 (MQ=255) ggggTTTGGGTTTTGTGAATGACTTTTTGGATCGCGGTCGCGTGAAAAAAGTCTATGTGCGGGCAGCTGCGCCGTACCGCATGCTGCCGGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 1:435318/239‑1 (MQ=255) ggggTTCGAGCTATGTGAATGGATTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTAGCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 2:443053/239‑1 (MQ=255) gggTTCGGGTTTTTTGAATGTCTTTATGGATGGCGGTCGCGTGAAAAAAGTCTTTGTGCAGGCACCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCGTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 1:591438/238‑1 (MQ=255) gggTTCGAGTTTTGTGAATGACTTTTTGGATCGGGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 1:581401/238‑1 (MQ=255) ttcgtgtgtgtgAAGGACTTTAGGGATCGCGGGGGCGTGAAGAAAGTCTGTGTGCAGGCAGCTGGGCCGTATCGCATGCTGCCAGATGACATCAATCCCTGGTATGTCCGAAATAAAGAGGGCGGCAGGGTGCCCTTCTCTGGTTTCGCGACCTCCCGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 1:774073/231‑1 (MQ=255) tCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 2:579939/239‑1 (MQ=255) tCGAGCTATGTGAATGACTTTAGGGATCGGGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACCTCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 2:361013/239‑1 (MQ=255) gCTATGTGAATGGCTTTAGGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGAATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAg < 2:533260/238‑1 (MQ=255) aTGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTa > 2:581401/1‑237 (MQ=255) tgtgAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCg > 2:4191/1‑239 (MQ=255) gtgAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAACTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCCCCGGGGGTTCGTACCgg > 2:341343/1‑239 (MQ=255) gAATGACTTTTTGGATCCGGGTCGCGGTGAGAAAATCTTGTTGACGGCAGCCGCCCCGTTTCGCAGGCTGCCAGATGACAACAATCTCTGGTGTGTCCGAAATAAAGAGGGCGGCATGGTGCCCTTTTCTGCTTTCTCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTa < 1:544297/239‑1 (MQ=255) gAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTTCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTa < 2:876759/239‑1 (MQ=255) tttgtggtgtCGCGGTTGGGTGAAGGAAGTTTTTGGTAAGGCGGATGCGCCGTATCGCATGCTGCCAGATGACACCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTGCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCg < 1:535562/229‑1 (MQ=255) ttttgggttCGGGGTCGCGTGAATAAAGTTTATGTGAAGGCGGCTGCGCGGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt < 1:63872/231‑1 (MQ=255) tttATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAACCAGG‑CTCCCCGCGTCTGGAACGCTATACCGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCGGCACCGGGGGCCAGGACAGGGACggg > 1:211370/1‑235 (MQ=255) gttgtgtcggtggtcgTTAAAAAAGTTTCTGTGCGGGCAGTGGCACCGTATCCCATGCTGCCAGATGACATCAACCTCTGGTATGTCCGAAATAAAGAGTGCGGCATGGGGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACATG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGTTCAGTACCGGTACGGCGATGGa < 1:420787/225‑1 (MQ=255) tCGCGGTCCCGTGAAGAAAGGCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGTAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt < 2:27174/238‑1 (MQ=255) cgcgGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTGGGGGAAGCCGACCCGGGGGTCCGTACCGGCACGGCGATGGAttttat > 1:125655/1‑239 (MQ=255) cgcgGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCCGTACCGGTACGGCGATGGAtattat > 2:776575/1‑239 (MQ=255) cgcgGTCCGGTGAAAAAAGCTTTTGTGCGGGAAGCTGCGCCGTATCGCAGGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCCCGGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtattat < 1:357758/239‑1 (MQ=255) ggTCGCGTGAAGAAAGTCTATGTGGAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGa < 2:124073/239‑1 (MQ=255) gcgTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCCGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATc < 2:460676/238‑1 (MQ=255) tgtgCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCGGGAACCCTATAACGGTTCTTCTGCGG‑TTGGGATTGTTGGGGAAGCGGCACCGGGGGTCGGAACCGGTACGGGGAGGGAGATTTTGGAATAGGTAGTGAAGAAGGTGc > 1:777889/1‑239 (MQ=255) catggcgCTTCGCCGTTTCGCAGTGTGACAAATGACAACAATCTCTGGTATGTCCTAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGTAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGTTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCaaa < 1:597565/233‑1 (MQ=255) caggcatgggcgcGGAATCGAACGCTGCCAGATGACCTCAATCTGTGGTAGGGCCGAAATAAAGATGGCGGCAGGGGGCCCATCTTTGCTTTCGGGACCGCCCGCGGGGAAACAGG‑CTCGCCGCGTCTGGAACGGTATAACGGTTATTCTGCGG‑TTGAGAGTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCaaa < 1:822913/230‑1 (MQ=255) gcagCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTTCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGAGCGGACCGGTCAGGCGATGGGTATTTGTGAATCGTTTGAGAAGCAGCCGCCAAACg > 1:442949/1‑238 (MQ=255) ctctggcgcgtagtgcttgctgcCAGAGGACATCAATCTCTGGTAGGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGCCCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCt < 1:170261/222‑1 (MQ=255) cccctgttCGCCTGCTGCCAGATGACCTCAATCTCTGTTACGGCCGAAATAATGACGGCGGCAGGGCGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGt < 1:780238/232‑1 (MQ=255) aTGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGa < 2:736728/238‑1 (MQ=255) gctgcCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACgg < 2:777889/239‑1 (MQ=255) tgcCAGATGACATAAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCg < 2:207000/239‑1 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt < 1:186601/158‑1 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt > 2:186601/1‑158 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGAAATCGTTAGTGACGCAGGTGCCAACGGGCTTTGGTGTGGGTGGGCCGGCAGTGTGGTATCAGGAGGGGCt > 1:763537/1‑239 (MQ=255) ggTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGCTTGGAGGGGACGGCGGTTTCGTATCAGGACCGGCtt > 2:385780/1‑239 (MQ=255) tGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGCTGGGTATTATGGAATCGTTAGTGAAGCAGCTGCCAAAAGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCCGGAGCGGCTTTCCg > 2:293966/1‑239 (MQ=255) gTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGCTATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCg > 2:35595/1‑238 (MQ=255) aaaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt > 1:546997/1‑154 (MQ=255) aaaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt < 2:546997/154‑1 (MQ=255) aaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGAGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCACACGGCTTTGGACTGGAGTGGACGGCGATTTGGTATAGGGAGGGGCTTTACGgggcgcc > 1:481076/1‑238 (MQ=255) gcggcATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTTTGGAGTGGACGGCGATGGCGGAACAGGAGAGGCTTTCCGGCGCGCAGGCCCGGgcg > 1:336931/1‑239 (MQ=255) tGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGTTCCGGCGCTGtttg > 2:758724/1‑234 (MQ=255) tgtCGCGACCCTACGTTGGGAAACCGGCCGCGCGGCG‑GTGGAACGCTTGCACGGTTATTCTGTGG‑TGGAGATGTTGGGGGAAGCCGCACCGGGGGTCAGTCCCGGTACGGCGATGGATTTTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGTTCCGTCGCTGTACGCCATTTCCTTGCtgg < 1:529589/237‑1 (MQ=255) tCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCCTTTCCTTGCtgggg > 2:119343/1‑237 (MQ=255) gcgACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTAACGGTCCGGCGATGGATATTTTGGAATCGTTAGTGAAGCAGCTTCCAAACGGCTTTGGGCTTGAGGGGAGGGCGAGGTCGAATCAGGAGCGGCTTTCCGGCGGGGGGGCACCGGGGCGGGCCGCCAATTCCtttgggggggg > 1:618243/1‑230 (MQ=255) gACCTCACGCTGGGAAGCGGG‑CTCGCCGCGTCGGGAACGCTATATCGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCTTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTa < 2:723449/238‑1 (MQ=255) cTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTTCGCCATTTCCTTGCTGGGGGGAtt > 2:134035/1‑237 (MQ=255) cTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATAAGGAGCGGCTTTCCGGCAACAAGGCTCCGGCGCTGTAGGCCATTTCCTTGCTGGTGGTCtt > 2:589535/1‑237 (MQ=255) ggggaacgcggcgcgcggctttgggAACGCTATAACGGTTATTAGGCGG‑TGGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTc < 1:756062/215‑1 (MQ=255) ggAAACAGG‑CTCGCTCGGTGTGGAACCCTTTAACGGTTTTTTGGCGG‑TTGAGATTGTTGGGGAAGCCGCCCCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTgg < 1:854153/239‑1 (MQ=255) gAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTgg < 2:200040/238‑1 (MQ=255) gggtctgccgcGTCTGGAACGCTATAACGGTTATTCTGGGG‑TTGGGATTGTTGGGGAAGCCGGCCCGGGGGTCAGTTCCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGGCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGt < 1:119343/233‑1 (MQ=255) ggggtccgtgtttgtGGAACGCTATGAGGGTTATTTGGGGG‑TGGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTgtgt < 1:776575/225‑1 (MQ=255) g‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGGATTCCTGGGTCTGGCTGCggtt > 2:756062/1‑236 (MQ=255) cgggccgcGTCGGGAACTGTATAAGTGTT‑TTCTGGGGTTTGGGATTGTGGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAAGCGGTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGGGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGt < 1:568912/233‑1 (MQ=255) tCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTa > 2:577139/1‑239 (MQ=255) ggAACGCTATAACGGTTAGTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGt < 2:211370/239‑1 (MQ=255) cTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGGTGTATGAAAGCTGGTCGGTg > 2:885136/1‑238 (MQ=255) caCGGTTATTGTGGGG‑GTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGtt < 1:54364/238‑1 (MQ=255) aaCGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGtt < 2:618243/239‑1 (MQ=255) cGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGGGTGGACGGCGAGGTCGTATCAGGAGCGGCTTTCCGGCGCCCGGGGCCCGACGCGTTACGCACTTTCCTTGCTGGTGGTCTTCCTGTGCTTGGCGGCGTGTTATCAAACCTGGTCGGTGCCGt > 1:619238/1‑236 (MQ=255) aTTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGGTGGCGGATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGGATTCCGGTGTCTGGCTGTGTTGTATGAAAGCTGGTCGGGGGCGTTCTCggga > 1:372315/1‑237 (MQ=255) aTTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTa > 2:825896/1‑239 (MQ=255) | ACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTA > NC_000913/2589831‑2590289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |