Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,676,479 G→C 14.0% G115G (GGC→GGG yphD ← putative sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,676,4790GC14.0% 184.7 / 2.5 77G115G (GGC→GGGyphDputative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (43/21);  new base C (5/8);  total (48/29)
Fisher's exact test for biased strand distribution p-value = 6.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGAT  >  NC_000913/2676251‑2676714
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
gaccacaAACAGCGCAAACAACCAAATCTGTACTAGCGCGTATCCCGGCACCCCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGc                                                                                                                                                                                                                                    <  1:65884/235‑1 (MQ=255)
     caAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGGGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCTCTCCACAGCCCCAGTGTGGCAACGAAACTTGCTACATTAAACACGCCCCGCAGCACTCCGGCCAGGGTTCCCATCACCGCCCCTAACACCacaacc                                                                                                                                                                                                                              >  2:618247/1‑234 (MQ=255)
                    acacAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACAACAGCAGGCACGcccc                                                                                                                                                                                                                >  2:161571/1‑236 (MQ=255)
                             tGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCACAACCAGCAGGCACGCCACCGCCAGCGGa                                                                                                                                                                                                      >  2:132419/1‑239 (MQ=255)
                             tGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCCCGCAGCACCCCCGCCAGCGTTCCCCTCCGCGCCCCTAACAGCAACACCAGCAGGCACGACACCGCCAGCgac                                                                                                                                                                                                      >  1:496997/1‑237 (MQ=255)
                             atgtccagccgggaacccggccacCGAGAAAATTGCCCGCCCGCCACGTCCAGCACCTCGTTTTCATCAACCGGCCCTGGCAGGGCGTTCGTCAAAAACAGCCCCATTCCGCGCAGGGCGCTCCCCAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCgg                                                                                                                                                                                                       <  1:132419/218‑1 (MQ=255)
                                    cgcgGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACCTTAAACACGCCGAGCAGCACCCCGGCCAGCGTTCCCCTCACCGACCCAAACACAAAAACCACCCGGACCACCACCGCCACGGGAACTTcca                                                                                                                                                                                               >  1:66354/1‑237 (MQ=255)
                                    cgcgGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGAAGCAACGCCACCGCAAGCGGAACTTCaa                                                                                                                                                                                               >  2:352080/1‑239 (MQ=255)
                                    cgcgGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCACCAGGCCCGCCACCGCCAGCGGAACTTCaa                                                                                                                                                                                               >  2:779850/1‑239 (MQ=255)
                                    cgcgGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCACCAGGCACGCCACCGCCCGCggcagtccaa                                                                                                                                                                                               >  2:20517/1‑231 (MQ=255)
                                    cgcgGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCCCGCAGCACCCCGGCCAGCGTTCCCACCACCCACCCTAACAGCCCCACCACCCGGCCCGcccacccccccggcaattcaa                                                                                                                                                                                               >  1:657078/1‑220 (MQ=255)
                                       ggATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAACCTTGGAACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCCCCTAAACCCAAACCCCACCGGCACCCCCCCGCCCCCGGACATTCaaagt                                                                                                                                                                                            >  1:733991/1‑237 (MQ=255)
                                                   acCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCCGCAGGCACGCCACCGCCACCGGAACTTCAAATTGCAGCAAAAAt                                                                                                                                                                                 >  2:881691/1‑238 (MQ=255)
                                                   acCGAGAAATTGCCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAAGCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACCCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATg                                                                                                                                                                                <  2:513933/239‑1 (MQ=255)
                                                         aaaaTTGCCCCCCCGGCCACTCCCGCACCTCGTTATCAAAAACGGGCACGGGCAGGGCGTTCGTAAAAAACAGCACCATTCCGCGCCGGGCGCGTCACAGCCTCAGTGTGACAACGAAACTGGGCACATAAAACACGCCCCGCAGCACCCCGGCCAGCTTCCCCACCACCGCGCCTAACAGCAACACCAGAAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAgg                                                                                                                                                                           <  1:874080/238‑1 (MQ=255)
                                                              tGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCACCGGAACTTCAAATTGCAGCAAAAATGCCAGGcaca                                                                                                                                                                       >  2:747632/1‑238 (MQ=255)
                                                                tCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAAATTCAAATTGCAGCAAAAATGCCAGGCACACCg                                                                                                                                                                    >  2:482167/1‑239 (MQ=255)
                                                                    cccAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAACGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAACGGAAATTCAAATTGCAGCAAAAATGCCCGGCACACCGAGAc                                                                                                                                                                >  1:823476/1‑239 (MQ=255)
                                                                               ccggctccTCGTTTTCATCAACCGGCACTGGCAGTGCGTTCGACATAACCAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGcc                                                                                                                                                         <  2:276024/230‑1 (MQ=255)
                                                                               ccAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCCCCGCCCGCGGAACTTCCAATTGCAGCAAAAATGCCAGGCACACCGAGAAAAAAAccaca                                                                                                                                                      >  1:756880/1‑238 (MQ=255)
                                                                                        cGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAACATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCa                                                                                                                                             >  2:107041/1‑239 (MQ=255)
                                                                                        cGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATCCCAGGCCCACCGAGACAAAAGCCACCATCGGccc                                                                                                                                              >  2:567934/1‑238 (MQ=255)
                                                                                        cGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCCCGCCTAACAGCACCACCAGCAGGCACGCCACCCCCAGCGGAACTTCCAATTGCAGCAAAAATGCCAGGCACACCGAGCCAAAAGCCACCATCGGCCCa                                                                                                                                             >  2:395658/1‑239 (MQ=255)
                                                                                            ttCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAACCCACCATCGGCCCAACGc                                                                                                                                         >  2:115068/1‑239 (MQ=255)
                                                                                                   aTCGGCACTGGCAGTGCGTTCGTCATAATCAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCCAAAATGCCAGGCACACCGAGACCACAGCCACCATCGGCCCACCGATGACATc                                                                                                                                  >  2:370977/1‑239 (MQ=255)
                                                                                                             gcggggCGTTCGTCAAAAACGCCCCCATTCCCCGCAGGGCCTCCCACAGCCCCAGTGGGGCAACAAAACTGGGCAATTAAAACCCCCCCGGCCGCCCCCCGGCCAGCGTTCCCACCAGCCGCCCTAACAGCAACACCAGCAGGCCCGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCAcc                                                                                                                         <  1:180360/233‑1 (MQ=255)
                                                                                                              cccttctttctgcaaaacccccccaATTCCGCGAAGGGCCCCCACCAGCCCCATTTTGGCAACGAAACTTGTTACATTAACCCGCCCGCGTAGCACCCCGGCCAGGTTCCCCACCAGCCCGCCGAACAGCACCCCCAGCCGGCCCGCCACCGCCAGCGGAACTTCAAATTTCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCTCCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  1:732366/218‑1 (MQ=255)
                                                                                                               tgTGCGTTCGTCAAAAACCCCCCCATCCCGCACAGGGCTCCCCAACACCCCAGGGTGGCAACGAAACTTGGTCAATTAACCACGCCGCGCACCCCCCCGGCCACCTGCCCCACCAGCGCGCCTAACAGCAACCCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  1:179731/237‑1 (MQ=255)
                                                                                                               cgTGGGTTCGTAAAAAACAGCCCCATGCCCGGCAGGGCCCCCCCCAGCCCCAGTGGGGCAACGAAACTTGGTCAATTAAACCCGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACCCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  2:112107/237‑1 (MQ=255)
                                                                                                               aGTGGGTTCGCCATAAAACGCCCCATTCCGCGAAGGGCGCTCCACAGCCCCAGGGTGGCAACGAAACTGGGTACATTAAACACGCCGCGCAGCCCCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  2:256709/238‑1 (MQ=255)
                                                                                                               aGTGCGTTCGTCAAAAACAGCCCAATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  2:828467/238‑1 (MQ=255)
                                                                                                               aGTGCGGTCGTAAAAAACAGCCCCATTCCGCGCAGGGCCCTCCACAGCCCCAGTGGGGCAACGAAACTGGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  2:733991/238‑1 (MQ=255)
                                                                                                                agtgctctcctaaaaaCACCCCCAATGCCGCCAAGGGCCGCCCACAGCCCCAGTGTGGCAAAGAACATTGGTACATAAACCCCGCCGCGCAGCCCCCCGGCCGGCCTTCCCTACACCGCCCCTAACAGAAACACCAGCAGGCCCCCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCgg                                                                                                                       <  1:523207/225‑1 (MQ=255)
                                                                                                                  gCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCAAGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGTCAAACCGAGACAAAAGCCCCCACCGGCCCAACCCTTCACTCAATTTCACCGGAGat                                                                                                                   >  2:860190/1‑239 (MQ=255)
                                                                                                                        gTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATaataat                                                                                                             >  2:330567/1‑239 (MQ=255)
                                                                                                                                  gCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGCGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAAACaaggccat                                                                                                    >  2:675156/1‑231 (MQ=255)
                                                                                                                                   ccccATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCCGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGcc                                                                                                                                               >  2:493970/1‑194 (MQ=255)
                                                                                                                                   ccccATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGcca                                                                                                                                                        >  2:131140/1‑185 (MQ=255)
                                                                                                                                   ccccATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATACCAGGCACACCGAGACAAAAGCCACCCACGGCCCAACCCTACCAACAATTTCACAGAGGAaaataaaaaagataaaac                                                                                                  >  1:22548/1‑227 (MQ=255)
                                                                                                                                         ttCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAg                                                                                             >  2:483691/1‑239 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTCAACACGCAGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCaact                                                                                                                                                                                             >  2:724893/1‑136 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCGAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCCGGCACGCCACCGCCAGCGGAACTTCAAATTGCCGCAAAAATGCCAGGCACACCGGGACAAAAGACCCCACCCGCCCAACGCCCACCACAATTTCACCGGAGATAAAAAACACCTCCATCACCCAgggagg                                                                                        >  1:813343/1‑235 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCCGGCACACCCAGACAAAAGCCACCATCGGCCCAACGCTCACATACATTTCCCCGGCGATAATAAACAGCGCCCACACCCAggccgc                                                                                        >  1:257379/1‑239 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCCCCGGGGATAATAATCAGCGTCATCGCCCAggcggc                                                                                        >  2:344215/1‑239 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAAAGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAggcggc                                                                                        >  2:797082/1‑239 (MQ=255)
                                                                                                                                              cgcAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAACCACCATCAGCCCAACGCTGACATCAATTTCACCGGAGATAAAAATCAACGTCACCGCCCAggcggc                                                                                        >  1:9287/1‑239 (MQ=255)
                                                                                                                                              cgcAGGGCGCCCCACAGCCCCGTTGGGGCAACGAAACTTGGTACATTAACCACGCCGCGCAGCCCCCCGGCCAGCGTTCCCATCAGCGCCCCTAAAAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAggcggc                                                                                        <  2:496997/239‑1 (MQ=255)
                                                                                                                                                   ggCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAACATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCCATTTCACCGGAGATAAAAATAAACGTCATCGCCCAGGCGGCAATcc                                                                                   >  1:111415/1‑239 (MQ=255)
                                                                                                                                                   ggCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATACTCACCGTCATCGCCCAGGCGCCAATcc                                                                                   >  2:102861/1‑239 (MQ=255)
                                                                                                                                                       cTCCACCGCCCAGTGGGGACAACGATATTTGTGACATCAAACCCGCCGCGCAGCACCCCGGCCAGCGTCCCCATCAGCCCGCCTAACGACAACGCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGAAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAt                                                                               <  1:206653/239‑1 (MQ=255)
                                                                                                                                                                  cAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATACTAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCa                                                                     >  2:6095/1‑238 (MQ=255)
                                                                                                                                                                         gCAACGAAGCTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCAAACGCCGAAATCAATTTCACCGGAGATAATAATAAGCGTCATCGCCAAGGCGGCAATCCCAAGGGGggcggaaccggcagc                                                             >  1:676077/1‑229 (MQ=255)
                                                                                                                                                                         gCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGGGGCAATCCCAACGGTGGCGGCATccccca                                                              >  2:860060/1‑233 (MQ=255)
                                                                                                                                                                         gCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGAAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCAGCGCgca                                                              >  2:541910/1‑238 (MQ=255)
                                                                                                                                                                            aaCGAACCGTGGCACATAAAACCCCCCCCGCAGCCCCCCGGCCGGCGTTCCCATCAGCGCCCCTAACAGCAACACCAGCAGGCACCCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCgcagca                                                           <  2:657078/239‑1 (MQ=255)
                                                                                                                                                                                aaccTTGGTACATTAAACCCCCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACTCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACg                                                         <  2:875707/234‑1 (MQ=255)
                                                                                                                                                                                  cctgggTACATTAAACCGCCCGCCCAGCCCCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCa                                                     <  2:756880/235‑1 (MQ=255)
                                                                                                                                                                                  acctGGTACATTAAACCCGCCGCGCAGCACACCGACCAGCGTTCCCATGCGCGCGCCTAACAGCAACCCCAGCAGGCCCGCCACCGGCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCa                                                     <  2:104460/236‑1 (MQ=255)
                                                                                                                                                                                  aCTTGGTACATTAAACCGCCCGGGCAGCCCCCCGGCCAGCGTCCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCa                                                     <  2:209023/239‑1 (MQ=255)
                                                                                                                                                                                   cTTGGTACATTAAACACGCCGCGCAGCCCCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCAt                                                    <  2:9287/239‑1 (MQ=255)
                                                                                                                                                                                                cccgccccccccgccccccGGCCGGCTTCCCCACCCGCCCGCCTAACAGACACCCCAGCGGGCCCCCCACCGCCAGCGGAACTTCAAATTTCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAt                                       <  1:409849/224‑1 (MQ=255)
                                                                                                                                                                                                cccccccgcccccccccccGGCCCGGCTTCCCTACACCGCCCCAAACAACAACACCGACAGCCCCCCCCCCGCCAGCGGAACTTAAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCAGCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAt                                       <  1:370096/224‑1 (MQ=255)
                                                                                                                                                                                                acccgccgcGCAGCACCCCGGCCAGCGTTCCCACCAGCCCGCCTAACAGCAACCCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAt                                       <  2:72836/236‑1 (MQ=255)
                                                                                                                                                                                                acacGCCCCGCCTCTCCCCGGCCAGGCTTCCCAACAGCGCCCATACCACCAACACAAGCCGGCACGCAACAGCCAGCCGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAAt                                       <  1:107041/239‑1 (MQ=255)
                                                                                                                                                                                                  acGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCCGGCGGCAATCCCAATGGGGGCGGACACGCCAAGCACCATCATCCGGGTATTCAATga                                     >  1:734945/1‑239 (MQ=255)
                                                                                                                                                                                                  acGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGCCGGCAATCCCAATGGTGGGGGCAACGCGCCGAACGTTCCTCTGGTTATTCactca                                     >  2:10655/1‑235 (MQ=255)
                                                                                                                                                                                                    gccgcGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCCACGCTAACATCAATTTCACCGGAGATAATAACCAGCGTCACACCCCAGGCCGCAAACCCAACTGGGGCGGCATCGCGCAGCCCGTTACTATGGTTATTCAAtagga                                   >  1:431213/1‑235 (MQ=255)
                                                                                                                                                                                                    gccgcGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCCTCGCCCAGGCGGCAATCCCAATGGGGGCGGCATCGCGCCGCACGTTCATCTGGTTATTCaaataga                                   >  1:228430/1‑234 (MQ=255)
                                                                                                                                                                                                    gccgcGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGACGGCAATCCCAATGGTGGCGGCATCGCGCAGACCGTTCCCCTAGGTATTCAATaagg                                   >  2:539873/1‑238 (MQ=255)
                                                                                                                                                                                                      cgcgCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCACTTTCACCGGAGCTAATAATCAGCGGAATCGCCCAGCCGGCAATCCCAATGGTGGCGCCATCGCGCAGACCGGTCGCCTGGGTTTTCAATgagaga                                 >  1:219785/1‑237 (MQ=255)
                                                                                                                                                                                                      cgcgCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAAtg                                                                              >  2:640563/1‑194 (MQ=255)
                                                                                                                                                                                                       gcgcAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTCATTCAATGAGaaaa                                >  2:273731/1‑239 (MQ=255)
                                                                                                                                                                                                              ccccgggcactcGTTCCCACAACCGCCCCAAACAACAACCCAAGCTGCCCGCCCCCCGCCACGGGAACTTCAAATTGCAGCAAAAATGCCAGGCACAACGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                         <  1:6095/228‑1 (MQ=255)
                                                                                                                                                                                                              cccccGGCGACGGTTCCAATCAGCGCGCCTAACAGCAACCCCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAgg                         <  2:813343/238‑1 (MQ=255)
                                                                                                                                                                                                                     ccAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTc                  >  2:94303/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccggcttCCCATCCCGGCCCCTACCCGCAACCCCGCCGGGCACGCAACCACCCGCGGAACTTCCAATTGCAGCAAAAATGCCAGGCACCCCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAAGCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCa                 <  1:483691/234‑1 (MQ=255)
                                                                                                                                                                                                                            tCCCATCAGCGCGCCTAACAGCCACACCAGCAGGCCCGCCCCCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGgag            <  2:629913/238‑1 (MQ=255)
                                                                                                                                                                                                                               ccccggccagcCCAACACCACCCCCAGCAGGCCCCCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCAACGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGagaaga        <  1:94303/230‑1 (MQ=255)
                                                                                                                                                                                                                                     cgcgcCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCCGGCGGCAATCCCCATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATCAGATAAAGCCAgggcggttaaggaggaaaacacaaa  >  1:445330/1‑216 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           
GAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACAGCCCCATTCCGCGCAGGGCGCTCCACAGCCCCAGTGTGGCAACGAAACTTGGTACATTAAACACGCCGCGCAGCACCCCGGCCAGCGTTCCCATCAGCGCGCCTAACAGCAACACCAGCAGGCACGCCACCGCCAGCGGAACTTCAAATTGCAGCAAAAATGCCAGGCACACCGAGACAAAAGCCACCATCGGCCCAACGCTGACATCAATTTCACCGGAGATAATAATCAGCGTCATCGCCCAGGCGGCAATCCCAATGGTGGCGGCATCGCGCAGCACGTTCATCTGGTTATTCAATGAGATAAAGCCAGGCGCGTTCAGGGAGAAGACCAGAT  >  NC_000913/2676251‑2676714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: