Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,682,535 A→C 20.2% V70G (GTG→GGG)  yphG ← DUF4380 domain‑containing TPR repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,682,5350AC20.2% 104.1 / 3.7 61V70G (GTG→GGG) yphGDUF4380 domain‑containing TPR repeat protein
Reads supporting (aligned to +/- strand):  ref base A (30/15);  new base C (4/9);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 2.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCC  >  NC_000913/2682305‑2682750
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                          
ccccccccGTCTGGGCCCCGTCTTCAGGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGAGGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATg                                                                                                                                                                                                                   <  1:79006/233‑1 (MQ=255)
     acCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTc                                                                                                                                                                                                              >  2:635557/1‑239 (MQ=255)
         tCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACGGCCCAGTTCCGGCAGGTTAATAACTTTGATGTATTCGtt                                                                                                                                                                                                           >  2:723423/1‑238 (MQ=255)
         tCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGttt                                                                                                                                                                                                          >  2:86913/1‑239 (MQ=255)
                 ccGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCCCCCGACCCCCCAGTTCCGGCAGGCTCATACCTTTGATTGAGTCGTTTTCACGCca                                                                                                                                                                                                  >  2:169203/1‑239 (MQ=255)
                 ccGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGGTGTAGTCGTTTTCCAGcc                                                                                                                                                                                                   >  2:73349/1‑238 (MQ=255)
                 ccGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGAACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGcc                                                                                                                                                                                                   >  2:629312/1‑238 (MQ=255)
                  cGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCCGGATCATCACTTTGATGTAGTCGGTTTCCAGCcac                                                                                                                                                                                                 >  2:79358/1‑239 (MQ=255)
                  cGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGATTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGTGCGATGCCACCCACCGCCCAGTTCCGGCCGGATCATCCCTTTGGTTGAGCCCTTTTCCAGCca                                                                                                                                                                                                  >  1:625376/1‑238 (MQ=255)
                   gTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCCGTTCCGGCAGGACCATCACTTTGTTGTGTTCGTTTTCCAGCcac                                                                                                                                                                                                 >  2:71752/1‑238 (MQ=255)
                    tCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAACGCTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGCAAATCCACAGCCCCCGCAGCCCCACCAGCGCAGGTTTACTGACTTCATTGTGCTAAACCAAATCGCGCTGGTTCCCTTTATCCCACGCGCGTGCCATCCACACGCCCAGTTCCCGCAGGACCATCACTTTGATGAAGACGTTTACCACCcaca                                                                                                                                                                                                >  1:791217/1‑238 (MQ=255)
                       tCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCACCACCg                                                                                                                                                                                             >  2:234092/1‑238 (MQ=255)
                             gttccggggggGAAAACAAGGGGCATAAAGGTGGTCGGGGGAGTGTGTTGGGCACAGGTAAACTCAATCCCACCGGAAACCCACGGCCCCAGCAGCCCCCCCAGGGCAGGTTTAATGACTGCATTGGGAAAACCAAAATCGCGCTGTTTCACTTTATCCCACCCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACCCCGCCGGCCa                                                                                                                                                                                      <  1:446272/232‑1 (MQ=255)
                              cTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCCCTTTGATGCAGTCGTTTTCCAGCCACACCGCCTGCCAg                                                                                                                                                                                     >  2:446272/1‑239 (MQ=255)
                              cTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAg                                                                                                                                                                                     >  2:205540/1‑239 (MQ=255)
                                               aCGGGCATAAAGGGGGTCGGGCGATGATGTTGCGGCCAGTTAAACCCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCAGTGTGATAAACAAAATCGCGGTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTg                                                                                                                                                                    <  2:520264/239‑1 (MQ=255)
                                                             ggtCGGGCGATGATGTTGCGGCCAGTTAAACTCCATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTCATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGAACTCCCAGTTCCGGCAGGCTCATCACTTTGTAGTAGGCGTTTTCCAGCCACAACGCCTCCCCGCGTTTCAGGGttttttacccgcccacggaat                                                                                                                                                      >  1:39833/1‑222 (MQ=255)
                                                             ggtCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCTGTGCACCCGACCGCCCAGATCCGGCAGGATCATCCATTTCATGTAGTCGTTTTcacgccccccccc                                                                                                                                                                                            >  1:668173/1‑189 (MQ=255)
                                                             ggtCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCACCGTa                                                                                                                                                       >  2:144777/1‑238 (MQ=255)
                                                             ggtCGGGCAATGATGTTGCGGCCAGTTAAACCCAATCCCCCCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTa                                                                                                                                                       <  2:136156/238‑1 (MQ=255)
                                                              gtCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAATCGTTTTACAGCCAAACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCCCAGCGTATc                                                                                                                                                     >  2:215608/1‑239 (MQ=255)
                                                                    gggtggtgTTGCGGCCAGTAAACCCCAATCCCCCCGAATATCCACGGCCCAAGCAGCCCCACCAGCCGAGGTTTAATGGTTTAATGGTGATAAACAAATTCGCGTTGTTTCACTTTACCCCACGGGCGGTGCCCCCGACCGCCCAGTTCCGGCAGGATAATCCCTTTGAGGTGGTCGTTTCCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCa                                                                                                                                                <  1:164597/232‑1 (MQ=255)
                                                                    caatgatgTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCCCCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCa                                                                                                                                                <  2:211175/236‑1 (MQ=255)
                                                                        tttttttcGGCCAGTTAATCTCAATCCCTCCGGAAACCCACGGCCCCAGTACCCCCCCCAGCGCAGTTTTAAGTACTTCATTGGGATAAACAAAACCGCGTTTTTTCACTTTATCCCTCGCGCGATGCCTCCGCCCGCCCAGTTCCGGCAGGATCATCCCTTTGATGTATGCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCg                                                                                                                                           <  1:79358/232‑1 (MQ=255)
                                                                        ttgtttGCGGCCCCTTTAACCCCACTCCACCCGAAATCCCCGGCCCCAGCCCCCCCCCCCCGGGAGGGTTAATGACTTCATTTTGAAAAACAAAAGCGCGCTTTTTCACTTTAGCCCACGCGCGATGCCCCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGGTGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCg                                                                                                                                           <  1:264967/235‑1 (MQ=255)
                                                                        tcgttggCGGCCATTTAAACCCAACCCCCCCGGAATCCCCCGGCCCAAGCACCCCCCCCCGCGCAGGTTTAATGTCTTCTGTTTTATAACCAAAACCCCGCTGTTTCCCTTTCCCCCACGCCCGATGCCCCCGACCGCCAAGTTCCGGCAGGATCATCCCTTTGATGTAGTCGTTTCCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCg                                                                                                                                           <  1:149061/233‑1 (MQ=255)
                                                                         atgTTGCGGCCATTTAACCCCAACCCCACCGGAAATCCCCGGCCCCAGAGGCCCCACAAGCGCAGGTTGAATGACTTCATTGTGATCAAAAAAATCGCGCTGTTTCACTTTCTCCCACGCGGGATGCCCCCGACCGCCAAGTCCCGGCAGGATCATCCCTTGGGTGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCg                                                                                                                                           <  1:98076/238‑1 (MQ=255)
                                                                           tttccGGCCAGTAAACCTCAATCCCACCGAAAAGCCAGGGCCCCGGCGGCCCCACCAGCGCTGGTTTAATGAATTCGTTGTGATAAACAAAATCCCGATGTTTACCTTTATCCCACGCGCGATGCACCCGCCCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACCCCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTa                                                                                                                                         <  1:732106/234‑1 (MQ=255)
                                                                             tGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCACCGTATCGGTCACGCCGGAggg                                                                                                                                      >  2:558948/1‑239 (MQ=255)
                                                                                       taaccccAACCCCCCCGGAAACCCACGGCCCAAGCAGCCCCACCGGCGCAGGTTTAATGACTTCATTGTGAAAAACAAAACCCGGTTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACcg                                                                                                                              <  2:863471/231‑1 (MQ=255)
                                                                                       tAAACCCAACCCCCCCGAAAATCCACGCCCCCAGACCCCCCACCAGGCCAGGTTTAATGCCTTCATTGTGATAAACAAAATCGCGCTTTTTCACTTTATCCCACGCGGGATGCCCCCGCCCGCCCAGTTCCGGCCGGATCATCACTTTGATGTGGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACcgcg                                                                                                                            <  1:86913/239‑1 (MQ=255)
                                                                                            tCAATCCCCCCGGAAACCCACTGCCCCAGAAGCCCCACCAGGGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGATGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGa                                                                                                                       <  2:17030/239‑1 (MQ=255)
                                                                                            tCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCGCGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGa                                                                                                                       <  2:437551/239‑1 (MQ=255)
                                                                                            tCAACCCCCCCGGAAATCCCCGGCCCCGGCAGCCCCACCAGGTCAGGTTTAATGACTTCATTGTGATAAACAAAACCGCGCTGTTTCACTTTACCCCACGCGCGATGCCCCCGACCGCCCAGTTCCGGCAGGATCATCCCTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGa                                                                                                                       <  2:149658/239‑1 (MQ=255)
                                                                                                tCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGa                                                                                                                    >  2:862947/1‑238 (MQ=255)
                                                                                                tCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCACCGTATCGGTCACGCCGTAGGGATAAACCGCGCGGGCCGa                                                                                                                    >  1:748787/1‑238 (MQ=255)
                                                                                                tCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGGTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCTCGCCGGACgg                                                                                                                    >  2:527635/1‑237 (MQ=255)
                                                                                                                       agcagcCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAACCGCGCTTTTCCAg                                                                                            >  2:702250/1‑239 (MQ=255)
                                                                                                                               cccccgcgtatttttAATGACTTTATGGTGAAAACCAAAATCCCGCTTTTTCACTTTAACCCACGGGCAAGGCCCCCGCCCCCCCAGTTCCGGCAGGATCTTCCTTTGGATTGAGTCGTTTTCCAGCCCCACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATgg                                                                                    <  1:635557/227‑1 (MQ=255)
                                                                                                                               ccccaGCTCAGGTTTAATGCCTGCTTGGGTATAAACAAAAGCGCCTTGTTTCCCTTTGCCCCCCCGGCGATGCCCCACACCGCACAGTTCCGTCAGGATCATCACTTTTATGTAGCCGTTTTCCAGCCACACCGCCTGCCAGGATTGCAGGGTTTTCTGCGCGCTCAGCGTATCGGCCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACTCGATTTTCCAGGAACATgg                                                                                    <  1:558948/237‑1 (MQ=255)
                                                                                                                               caccaGCGCAGTTTTAATGCCTTCATTGTGATAAACAAAACCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATgg                                                                                    <  2:748787/239‑1 (MQ=255)
                                                                                                                                                 gccttcttTGTTAAAAAAAAACTCCCGTTTTTTCCTTTTTCCCCCCGCGGGATCCCCCCGCCCGCCAAGTCCCGGCCGGTTCATCACTTTGATGTAGTCGTTTCCCAGCCACCCCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGcc                                                                  <  1:144777/237‑1 (MQ=255)
                                                                                                                                                   cTTCATTGGGAAAAACAAAACCGCGCTGTTTCACTTTATCCCACGGGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTGGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGccc                                                                 <  2:706533/238‑1 (MQ=255)
                                                                                                                                                    ttCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCCCACCGCCTGCCAGGTTTTCAGGGTTTTCTGCTCGCTCCACGTATCGGTCACGCCGTATGTGTCAACCTCGCCGGCCCATCCCGGATAAACCCGATTTTCCCGGAACATGGGGTGTAAATCCTGCGGCCCgg                                                               >  1:454009/1‑239 (MQ=255)
                                                                                                                                                    ttCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGCTAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCgg                                                               >  2:39889/1‑239 (MQ=255)
                                                                                                                                                    ttCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCGGTAAAACGCGATTTTCCAGGAACAAGGGAGGGATATCCTCCGGCCCgg                                                               >  1:1436/1‑239 (MQ=255)
                                                                                                                                                      cATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGCTAAACCGCGCCGGACGATCCCTGCTAAACGCGATTTTCCGAGACCATGGGAGGTAAATCCTGCGGCCCggct                                                             >  2:777301/1‑237 (MQ=255)
                                                                                                                                                                     aaTCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGCTCCCTGATAAACGCGATTTTCCAGGAACATGGGAGGTATATCCTGCGGCCCGGGTTACTAGGCCGGGaa                                               >  1:679603/1‑237 (MQ=255)
                                                                                                                                                                     aaTCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAAAATGGGAGGGAAATCCTGCGGCCCGGTTTCATAGGTggggga                                               >  1:885710/1‑236 (MQ=255)
                                                                                                                                                                          cgcTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAACCGCGATTTTTCAGGGACATGGGAGGCGTATATATCGGCCCGGTTTccatagtagggatccaaaa                                         >  1:357846/1‑221 (MQ=255)
                                                                                                                                                                                       ttcccccCGCGGGTTGCCCCCGCCCGCCCAGTCCCGGCAGGGTCGTCTTTTTGATGGCGGCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCCCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCcac                          <  1:39889/236‑1 (MQ=255)
                                                                                                                                                                                         tCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGTTAGCTCAGCGTATCGGTCACGCCGTAGGGTTAAACCGCGCCCGACGATCCCTGATAAACGCGCTTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGCTGTCATCGGTCGGGACTCTCACGCGATCTTGCcac                          >  2:349612/1‑237 (MQ=255)
                                                                                                                                                                                         tCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGCGGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCcac                          >  2:425231/1‑237 (MQ=255)
                                                                                                                                                                                         tCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGAGGTAGTCGTTTTCCAGCCACACCGCCGGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACCCCGCAGGGATAACACGCGCCGGACGCTCCCTGATAAACGCGTTTTTCCATGAACATGGGACGCATATCCTGCGGCCCGGTTTCCTAGGTCGGGGTCTCAACGCGCCCTTGCcac                          >  1:421448/1‑237 (MQ=255)
                                                                                                                                                                                               gcgcctcacccccTACCCCCCAGTCCCGGACTGATCATCCTTTTGAGTGTAGCGGTTTCCACCCACACCCCCTGCCCGGGTTTCAGGGTTTTCTGCTCGCTCAGCGTTACGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACGCTTTTAc                  <  1:862947/229‑1 (MQ=255)
                                                                                                                                                                                                    ttGCACCCGTCCCCCCCTTTCCGCCATGATAATCTCTTTGATGGTGTCGTTTTCCAGCCACCCCGCCTGCCAGGATTTCAGGGTTTTCTGCCCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAg             <  1:205540/238‑1 (MQ=255)
                                                                                                                                                                                                          ccGACCGCCCAGTTCCGTGAGGTGTTTCTCTTGGTGGTAGTCGTTTTCCAGCCACCCCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATgg       <  2:830219/239‑1 (MQ=255)
                                                                                                                                                                                                           ccccccccacttcccggggggATCGTCCTTTTGATGGAGTCGTTTTCCAGCCGCCCCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGGTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGt      <  1:757788/220‑1 (MQ=255)
                                                                                                                                                                                                             aCCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGCCGATCCCTGCTAAACGCGATTTTCCAGGAACATGGGATGAATTTCCTCCGGCCCGGTTTCCTTGGTCGGGATCTCAACCCGCTCTTGCCCCACTTTTTCTGGGGTctggggt     >  1:559524/1‑232 (MQ=255)
                                                                                                                                                                                                             aCCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGGTAAACGCGATTTTCACGGAACATGGGAGGTATATCCTGCGGCCCGGTTTATGAGGCCGGGATCTCAACGCCGCTTTGCCCCACTTTTACTGGGGACgtggtg     >  1:864429/1‑238 (MQ=255)
                                                                                                                                                                                                               cGCCCAGTTCCGGCAGGATCATCCTTTTGATGTAGTTGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGccc  <  2:885710/239‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                          
CCCACACCGTCTGTGCACCGTCTTCATGGGCTTCGAGGGTGAAATCAACGGGCATAAAGGTGGTCGGGCGATGATGTTGCGGCCAGTTAAACTCAATCCCACCGGAAATCCACGGCCCCAGCAGCCCCACCAGCGCAGGTTTAATGACTTCATTGTGATAAACAAAATCGCGCTGTTTCACTTTATCCCACGCGCGATGCACCCGACCGCCCAGTTCCGGCAGGATCATCACTTTGATGTAGTCGTTTTCCAGCCACACCGCCTGCCAGGATTTCAGGGTTTTCTGCTCGCTCAGCGTATCGGTCACGCCGTAGGGATAAACCGCGCCGGACGATCCCTGATAAACGCGATTTTCCAGGAACATGGGATGTATATCCTGCGGCCCGGTTTCATAGGTCGGGATCTCAACGCGCTCTTGCCACACTTTTACTGGAGTCATGGTGCCC  >  NC_000913/2682305‑2682750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: