| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,265,358 | T→C | 18.4% | G223G (GGA→GGG) | tdcB ← | L‑threonine dehydratase, catabolic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,265,358 | 0 | T | C | 18.4% | 161.6 / 3.6 | 75 | G223G (GGA→GGG) | tdcB | L‑threonine dehydratase, catabolic |
| Reads supporting (aligned to +/- strand): ref base T (45/13); new base C (4/12); total (50/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.79e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT > NC_000913/3265123‑3265593 | gAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCg < 2:753453/239‑1 (MQ=255) aaTATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGTTTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGGTA‑TTTCCCCga > 1:555507/1‑238 (MQ=255) tatatTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAg < 2:466326/239‑1 (MQ=255) atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGCCCAATTCACTAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGCGCCATCCCAACCAT‑CCGCCAGGGGGCCGGTAGTACGGGGCGTGGTTA‑TTTCTCCGGAgggg > 1:421188/1‑239 (MQ=255) atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTgg > 2:455260/1‑239 (MQ=255) atTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGGGCGTGGTTA‑TTTCTCCGGAgggg > 2:341152/1‑239 (MQ=255) tGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGGGTGGaa > 2:709339/1‑239 (MQ=255) tttattttCCCGTTTAAAAAGGCGGCCCAGGCCAG‑GCGCCCGGGCCTTTCGGTGACGACTTTATTGCGCGGAATTAAGGCAAACATTCTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAaaga < 1:433810/234‑1 (MQ=255) tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCCAGAACGATTTCTTAAGTTACATT‑ACCCGGGCGGGAGCAATCACAACCAA‑CCGCCAGGGGGCCGTAAGTTCGG‑GCGGGGGTATTTTCTCCGGAGTGGaaaaa > 1:829867/1‑237 (MQ=255) tCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTCCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCCCAACCAT‑CCGCCAGGGTGCCGGTAGTTAGGTGCGTGGTTA‑TTTTTCCGGAGTGGAaaga > 2:402298/1‑239 (MQ=255) ccGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGGGCTGGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCAt > 1:610376/1‑239 (MQ=255) cGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑CTGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTAGGTGCGTGGTTA‑TTTCTCCGGAGTGGAGAGAAGCCGCCAt > 2:522973/1‑238 (MQ=255) cGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCCCAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGGGTGGAAAGAAGCCGCCAt > 2:349611/1‑238 (MQ=255) cTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGCGCCATCACAACCAT‑CCGCCAGGGGGCCGTTAGTCCGGACCGAGGGTA‑TTTATCCGCCGTGGCAAGAGGCCGCCATGcc > 2:474228/1‑239 (MQ=255) aataatGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCCCAACCAT‑CCCCCAGGGTGACGGTAGTTCGGTGCGTGGTTA‑TTTCACCGGAGTGGAAGCAAACCGCAAAGCCggg > 1:40498/1‑237 (MQ=255) aataatGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGGGCCGGTAGTTCGGGGCGTGGTAT‑TTTTCCCGGGGGGGAAAAAAGCCGCCATGcgcgc > 1:275416/1‑235 (MQ=255) tGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGATTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCTTAAGTTAAATT‑ACCCGGGCGGGAGCCATCCCAACCAT‑CCTCCAGGGTGCCGGTAGTTCGGGGCTTGGTTA‑TTTTTCCGGAGGGGAAAGAAGCAGCCTTGCCGGGAACGt > 2:77570/1‑239 (MQ=255) agcaCATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGCGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCTGGAACGtttt > 1:197663/1‑239 (MQ=255) agcaCATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACACGGGCGGGAGACATCACAACCAT‑CCGCCAGGGGGCCGGTAGTTCGGGGCGTGGTTA‑TTTATCCGGGGTGGAAAGAACCCGCCCTGCCGTGGACGttt > 1:13755/1‑238 (MQ=255) cacaTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCACTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGGGCGTGGTTC‑TTTCTCCGGGGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa > 2:654462/1‑239 (MQ=255) cacaTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTTACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGCGCCGGTAGTTCGGCGCGTGGTTA‑TTTCTCCGGTTTGGAAAGAAGCCGCCATGCCGTGAACGTTTTc > 2:767042/1‑238 (MQ=255) cacaTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGCGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGAGGGTGGTTA‑TTTTTCCGGAGTGGAAAGAAACCGCCAGTCCGGGAACGttttaa > 1:713937/1‑237 (MQ=255) cacaTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCCCAACCAT‑CCGCCAGGGTGCCGGTGGTTCGGTGCGGGGTTA‑TTTCCCCGGAGGGGAAAGAAGCCGCCATGCCGGGACCGTTTTCa > 2:394392/1‑239 (MQ=255) cacaTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa > 2:370422/1‑239 (MQ=255) tcaTGCCGGAGCGCCTTCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATTCTGTTTCTTATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa < 2:708628/237‑1 (MQ=255) acaTGCCAGAGCGCCTGCGCC‑TTCGGGGACGACTTTAGTGAGCTTAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑AGGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCCCAACCAT‑CCGCAAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCa < 2:72659/238‑1 (MQ=255) caTGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAAACAT‑CACCCAGGGTGCCGGTGGTTCGGTGCGTGGTTA‑TTTGCCCGGAGCGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGa > 2:497866/1‑239 (MQ=255) agCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGc > 2:296652/1‑239 (MQ=255) cttgcttc‑tTCGGTGCGCATTTTTTTGCGCGAAATTAAGGCAATCAATATTTTTCTGATTTTGTCTTCGGTGCCCAGCAGGTGTGTCA‑CGAATATTTCAGGAACGTTTTGGAAAGTTAAATT‑ACCCGGGCGGGAGACATCAAAACCAG‑CCCCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAAt < 1:493257/232‑1 (MQ=255) ctgcgcc‑tTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCTGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATa > 2:306737/1‑239 (MQ=255) ctgcgcc‑tTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCCAATa > 2:870416/1‑239 (MQ=255) gaacgacTTTATTGCGATGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGt < 2:668946/236‑1 (MQ=255) cgcTGAATTAAGGCAATCATACTGTTTCTGTTTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAGGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACACCAATAACACGGATGGTCGGGTTAATGAAttt > 2:294068/1‑239 (MQ=255) cgcTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCACATAACACGAATGGTGGGGCTAAGAGAttt > 2:448064/1‑239 (MQ=255) aTTAAGGCATTTATTCTTTTTCTGATTTCTTGTTCGCTTACCAGCCCG‑ATTTCATCGACAATTTCACGAACGTTTTCGTAAGTTAAATT‑CCCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCCCCGGAGGGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGc < 1:520224/239‑1 (MQ=255) aTTAAGGAAATCATACTGTTTCTGATTTCGGCTTCGCTGACCAGCACG‑ATGTCAACGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGc < 2:611046/239‑1 (MQ=255) aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCCCaa > 2:462290/1‑238 (MQ=255) aTCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAAACCGCATGGGTGGGTTAAAAGATTTAAtttcacccccaaa > 1:790026/1‑228 (MQ=255) tttCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACAAGCAAt > 2:559793/1‑239 (MQ=255) ttgtttttcggtgcccgccACG‑AGGTGAGCCACTAGTTCAGGACAGATTTTGAAAGTTAAATT‑CCCGGGGCGGGAGACCTCACAACCAT‑CCGACGGGGCACCGGTAGTTCGGGGGGTGGTTA‑TTTACCGGGAGTGGAAAGAAGCCGAAATGCCGGGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATGGCCACCGCAATACCAGCAATTAAACCGc < 1:586601/221‑1 (MQ=255) cGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGCCTGTACGCCAATAAAACGAATGGTCGGGTTAAAAGATTTAATTGCCACCGCAATAACAGCAAATAAACCGcca > 1:181948/1‑239 (MQ=255) cGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGca > 2:158942/1‑237 (MQ=255) aCCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACGACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCACTAACACGCATGGCCGGGTTAAAAGATTTAATTGCCACCGAAAACACACCAATTAAACCGcccccacacatggg > 1:337625/1‑232 (MQ=255) cacttGTCATCGAATTATTCCCGAAAGATTTCGTAAGTTAAATT‑CCCGGGGGGGGACAAATCCAAACACA‑CCGACGGGGTGCCGGTGGTTGGGTGCGTGGTTT‑TTTCCCGGGAGGGGAAAGAACCCGCCATGCCGTTAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAAt < 1:709339/235‑1 (MQ=255) tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCg < 2:205225/107‑1 (MQ=255) tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCg > 1:205225/1‑107 (MQ=255) tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCCCCACCAATTGGCACAAACACg > 2:151813/1‑239 (MQ=255) tGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACg > 2:281516/1‑239 (MQ=255) ttcagcggattaTTCACGAACGATTTCGAAAGTTAAGTT‑CGCCGGGGGGGAGACCGCACAAACCA‑CCGCGGGGGTGCCGGGAGTTCGGTGCGTTGTTA‑TTGCCCCGGGGTGGAAAGAGGCCGCCACGCCGTGAACGTTTTCAGACTGTACGCCAATACCACGAATGGTAGGGTTAATAGATTTAATTGCCCCCGCAATCCCAGCAATTAAACCGCCACCACCAATTGGCACAATCACg < 1:754442/227‑1 (MQ=255) aTCGACTAATTCACGACCGTTTTCGAAAGTTAAATT‑CCCCGGGCGGGAGACCTCCAACCCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTAt < 2:610376/239‑1 (MQ=255) tCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACAACCAATTGGcaacaaaaccttat > 1:403639/1‑226 (MQ=255) tCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCACCCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAACTACGGTATc > 2:418916/1‑239 (MQ=255) tCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGGGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGCGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAAAAGATTTAATTGCCAACGCAAAACCAGCAATATAACCCCCACCACCAATTGGccaaaaaccgttata > 1:759444/1‑225 (MQ=255) gtctaattccgaaacattttGGTAATTTAATTT‑CCCCGGGCGGGAGACATCACAACCAT‑CCGCCGGGGTGCGGGTAGTCCGGTGCGTGGTTA‑TTCCTCCGGAGGGGAAAAAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGa < 2:762585/222‑1 (MQ=255) gtatAATTCACGAACGTTTCCTTAAGTTAAATG‑CCCCGGGCGGGACAATTCACACCCCT‑CCGCCAGGGTGCCGGTAGTCCGGTGCGTGTTTA‑TTTCCCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCGGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGa < 2:56291/236‑1 (MQ=255) gcaaaTTCAGGAACGTTTTCGTAAGTAAAATT‑CCCCGGGCGGGAGAAATCACACCCAG‑CCGCAAGGGGGCCGGTAGTGCGGTGCGGGGTTA‑TTTCTCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGa < 2:790026/235‑1 (MQ=255) ttatttCACGAAGGATTTCGTAGGTTAATTT‑CCCGGGGCGGGAGACATCAAAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGAc < 2:713937/234‑1 (MQ=255) tAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCCCCAATTGGACAAATCACGTTACCGaaa > 1:513294/1‑236 (MQ=255) aTTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGAcatca > 2:229874/1‑239 (MQ=255) aTTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGAAATTAAACCCCCACAACCAATTGTCACAATCACGTTATCTCcatct > 2:560664/1‑238 (MQ=255) tttCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGAATTAATTGCAACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGCCACAAAAAAGATCtcca > 1:589306/1‑236 (MQ=255) ttagaGTTGAATTGACCGGGGGGGGAAACATC‑CAACCCTGCCGAGGGGGGGCCGGGAGTTCGGTGCGGTGTTT‑TTTGCCGGGGGGGGAAAGAAGCCGCCATGCCGGGAACGTTTTCAGACTGTACGCCAATAACACAAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCAt < 1:281516/235‑1 (MQ=255) cGTAAGTTAAATT‑CCCCGGGGGGGAGACATCACAACCAT‑CCGCCGGGGTGCCGGTAGTTGGGTGCGTGGTTA‑TTTCCCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATa < 2:275416/239‑1 (MQ=255) gTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCACTAAttt > 2:84423/1‑238 (MQ=255) gTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACACCCAATTGGCACAATCACGTTATCGACATCATAGAGACCTCCCAAAATTTc > 1:624078/1‑239 (MQ=255) cccGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCAACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCAAAATCACGTTATCGACATCATAGAGATCTTCCAAAATTTCccaaccaa > 1:414964/1‑238 (MQ=255) tCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAAAGATCTTCCATAATTTCCAAACCAATCGTCCCCTGGCCAGc > 1:612259/1‑239 (MQ=255) caacccct‑CCGCCAGGGGGCCGGTGGTTCGGTGGGTGGTTA‑TTCCCCCGGAGGGAAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCaa < 2:181948/232‑1 (MQ=255) t‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACtt > 2:45779/1‑238 (MQ=255) t‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCAACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAACCGTTCCCTGGCCAGCAATCACttc > 2:793343/1‑238 (MQ=255) ggttttttgggTGCGTTTTTT‑TTTCCCGGGGGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGTCTGTACGCCAATAACCCGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTaa < 1:45779/230‑1 (MQ=255) ttttgggggggtgttttttttCCCCGGGGAGAAAAGAAGCCGCCATGCCTTAATCGTTTTAAGACTGTACCCCAATAACACGAATGGACGGTTTAATAGATTTAATTGCCACCGCAATACCAGCAATAAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAgg < 1:84423/220‑1 (MQ=255) gttgggggggtgtTTA‑TTTCTCCGGAGGGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAAATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTgg < 1:158942/230‑1 (MQ=255) gggTGGGGGGTTA‑TTGCCCGGGAGGGAAAAGAGGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGAt < 2:13755/238‑1 (MQ=255) tttCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGCCATCATAGAGATCTTCCATAATTTCCAGACACATCGTTCCCTGGCCAGCAATAACTTTCGGATCACCGTAAGGTGGAGTAAAAATACGGcc > 2:763565/1‑238 (MQ=255) tctcCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCtt > 2:595886/1‑238 (MQ=255) | GAATATATTGGTCTAATTTACCGCTTAATAATGCAGCACATGCCAGAGCGCCTGCGCC‑TTCGGTGACGACTTTATTGCGCTGAATTAAGGCAATCATACTGTTTCTGATTTCGTCTTCGCTGACCAGCACG‑ATGTCATCGACTAATTCACGAACGATTTCGTAAGTTAAATT‑ACCCGGGCGGGAGACATCACAACCAT‑CCGCCAGGGTGCCGGTAGTTCGGTGCGTGGTTA‑TTTCTCCGGAGTGGAAAGAAGCCGCCATGCCGTGAACGTTTTCAGACTGTACGCCAATAACACGAATGGTCGGGTTAATAGATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTT > NC_000913/3265123‑3265593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |