Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,293,765 | C→G | 19.5% | A123G (GCG→GGG) | lpoA → | OM lipoprotein stimulator of MrcA transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,293,765 | 0 | C | G | 19.5% | 115.5 / 5.8 | 75 | A123G (GCG→GGG) | lpoA | OM lipoprotein stimulator of MrcA transpeptidase |
Reads supporting (aligned to +/- strand): ref base C (14/42); new base G (6/9); total (21/54) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.33e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.28e-01 |
AGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTC > NC_000913/3293528‑3293998 | agggttgtttggCCTTTTTTCTTCAGGGAGTGCAGCAAAGCTCGGAGGAATCCAGGATCAACTGGCAATTACTCGCCATTCGGGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑Gc < 1:197467/227‑1 (MQ=255) ttCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGGGAAAA‑CA‑CTGCTGGCGGGAAGGATTAAAC‑TGGCGCCAGAAGAATTTTCTGGCG‑CGCAAAACATGCTT‑GGGGAAAtta > 2:137933/1‑237 (MQ=255) ccTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTAACGCAAGAACCGAAACATGCGCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGAGAAAACACGGCGC‑GAAAGATTTTGCTGTGG‑CGCCAAAAATGTTG‑CGGAAAAAA‑ACAccc > 1:721472/1‑238 (MQ=255) tttttctttCAGCATATTCAGAAAAGCTTGTATGTTACAAGGATCACCTGGAAATTACTCGCCTTTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGc < 1:216468/232‑1 (MQ=255) tCTTCAGCAGATGCAGGAAAGCTCTGATGATACCAGGATCAACTGGCAATTCCCCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGAtt < 2:843895/239‑1 (MQ=255) tCTTCAGCAGATGCAGCAAAGCTTTGATGATCCCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGAtt < 2:8246/239‑1 (MQ=255) tCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGc < 2:743126/234‑1 (MQ=255) aaaGCTTTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGc < 2:207008/237‑1 (MQ=255) aGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGGGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAgc < 2:233271/239‑1 (MQ=255) ctctGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCCCCGAAAGATTTTGATGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACCAAACC‑ACCAAgccc > 2:449753/1‑237 (MQ=255) tgatgaTACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCCGGCGGTTGAGTTGTTTAA‑ACCACAACCGCAAGAACTGAACGATGCTCAC‑AGTCGCGCGAAAA‑CA‑CTGCTGGCGGTAGGGGGTAAAC‑TTGCGCCGAAAAAGTTTGTGTGCT‑CGCAAAACATGCTGAGGCAAAAAC‑A‑ACCAGCCCATTTAAAACaaaaaaacaaaaacccgt > 1:372113/1‑225 (MQ=255) atcccAGGACCAACTGGAATTGTCCCCCCATTCGGGCACGGGTAAAAGAGGGTAAAACCGGGCAGGGTGGTGAGGTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCCG‑CGTCGCGAGAAAA‑CC‑CTGCGTGCGGTAGAGATTAACC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCAAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTgg < 1:792803/236‑1 (MQ=255) gATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTAACGCAAGAACTGAACGATGCTCAG‑CGTTGCGGGAAAA‑CA‑ATGCTGGCGGTAAAGATTTAAC‑CTGCGCCAAAAGAATTTTGTGGCG‑CGCCAAACTTTCTT‑GGGGAAATC‑CCACACCCCCGTTTTGAAAAAAAAC‑AACAAACGCGCTAATgggaagaaa > 1:224150/1‑232 (MQ=255) aaCTGGCAATTTCTCGCCATTCGTGCACTGGTGAAAGAAGGTAAACCCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAAt < 2:712039/239‑1 (MQ=255) aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGCTGCTCAC‑CGTCGCGAGAAAA‑CA‑ATGCTGGCGGGAGAGATTAAAA‑TAGGGCACAAAGATTTTGTTCGCGCCGCAAACATCGGTG‑G‑GCAAAAC‑ACACCCGCCCGTTTTGAAAAAAACCAAAAAAGCAGGTAAATGGCAGGaaaaaaa > 1:805455/1‑238 (MQ=255) aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGGCCCGAGAAAA‑AACCTCCTGGGGGAGGAGTTAAAAC‑GGGCAC‑GAAAGAATTTTGTGCGC‑CGCAAAACATGCTG‑GCGAAAAAA‑CCACCCGCCGATTTAGAAAAAAAAC‑CACAAACGCGGTACTggggaggaaaaaaa > 1:458674/1‑227 (MQ=255) aCTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAAATGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAA‑CTGCGCCGAAAGATTTTGCTGGCG‑CGCAAAAATTTCTG‑GGGAAAAACAACCCCTCCCATTTTAAAAAAAAACC‑AACAAACGCGTAACGGGAGGGCaaaaaa > 1:743806/1‑238 (MQ=255) cAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑Tg < 2:106803/238‑1 (MQ=255) cgcGCCATTGGTGCACGGGTAAAAGAAGGTAAAGCCGGGCAGGGGGTTGAGTTGTTTAA‑CAAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CC‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑Tgccagcca < 2:872951/237‑1 (MQ=255) tCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAAAGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGGGGAGGATAAAA‑TTGCGAAGGAAAATTTTGTTTGGC‑CCCAAAACCTGCTG‑GGGAAAAAA‑ACAACTGCCGATTTATAACAAAAAC‑AAAAAGCCGGGTACTGGCAGGCaaaaaaaagaaacaaccag > 1:644844/1‑225 (MQ=255) ttaTTCGGGCACGGGGGAAAGAAGGAAAAAGCGGGGAGGCGGTTGAGTTGTTTAA‑CCACCTCGCGAAAAAACTGAAGGATGCCCGG‑CGTCCCGAAGAAA‑CC‑TTGCTGGCGGGAGAGTTTAAAC‑TGGCGGAGAAAGATTTTGCTGGCG‑CGAAAAACTTGCTG‑GGGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAgggg < 1:641815/237‑1 (MQ=255) ccttgcGTGCCCTGGTAAAAGAAGGTAAACCCGGGCAGGCGGTTGGGTTGTTTAA‑CCAACTACCGCAAGAACTAAACGATGCCCGG‑GGTCGCGAAAAAA‑CC‑CTGCGGGGGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGAAAAACTTGCTG‑GGGAAAATC‑CCACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAgggg < 1:20834/234‑1 (MQ=255) tCGTGCACTGGTGAAAGAAGGTAAAGCCGGGCAGGCGGTTGAGTTTTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTc < 2:863684/239‑1 (MQ=255) tCGTGCAATGGTGAAAGAAGGTAAAACCGGGCAGGGGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTc < 2:321545/239‑1 (MQ=255) gCATTGGTGAAAGAAGGTAAAACCGGGAAGGCGGTTGAGTTTTTTAA‑CCAACTACCGGAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTc < 2:843954/239‑1 (MQ=255) ttggggtaaaaagggTAAAACCGGGAGGGGGGTGGGGGTTTTTAA‑ACAATTTCCGCAAGAACGGAACGATGCGCAG‑CGTCGCGAGAAAA‑CC‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GGGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCa < 1:71170/226‑1 (MQ=255) tgtGGTGAAAAAAGGTAAAAGGGGGCAGGGGGTTTGGTTTTTTAA‑CCATCTACCGCAAGACCTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGAGTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCa < 1:253996/237‑1 (MQ=255) ccgggTGAAAAAAGGTAAACCCGGGCAGGCGTTTGGGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTAAG‑CGTCGCGAGAAAA‑CA‑CGGCTGGAGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GGGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCa < 1:300176/236‑1 (MQ=255) cTGGTGAAAGGAGGTAAAACCGGGCCGGGGGGTGGGTTGTTTAA‑CCAACTACCGGAAGAATTGAAGGATGCTCAG‑CGTAGCGAGAAAA‑CA‑GTGCTGGGGGTAGAGATTAAAC‑TGGCGCAGAAATATTTTGCGGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCa < 2:737096/238‑1 (MQ=255) gTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAATTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGTTGGCGGTGGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAAc < 2:511995/180‑1 (MQ=255) gTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCCGCCGATTTAGAACAAAACC‑AGCAAACGCGTTACTCGCAGGCAAAAAACGA‑TGCCCCCCAAGGGCGTCCTTCCactttttttctacc > 2:843835/1‑225 (MQ=255) ttaaaCGGGGCAGGGGGTTGAGTTTTTTAA‑CCAACTCCCGAAAGAACTGAACGGTGCCACG‑TGTCCGGAAAAAA‑CG‑CTGCGGGCGGAAGAGATTAAAC‑GGGCGCAGAAAGATTTTGCTGGCG‑CGAAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg < 1:648640/235‑1 (MQ=255) caaaaCCGGGCCGGCGGTTGGGTTTTTTAA‑CCACATACCGCAAAAACGGAACGATGC‑CAGAGGTCCCGAGAAAA‑CC‑CGGCTGGCGGTAGAGATTAAAC‑TGGCGCAAAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg < 1:777828/236‑1 (MQ=255) aaaaaCCGGGGAGGGGGTTG‑GTTTTTTAACCCAACTGCCGGAAGAAATGAACGGTGCCCAG‑GGCTGGAAGAAAA‑CC‑CTGCTGGCGGTAGGATTTAAAC‑TGGGGCAGAAAGATTTTGCTGGCG‑AGAAAAACTTGCTG‑GGGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg < 1:748880/236‑1 (MQ=255) aaaCCGGGCAGGCGGTTGAGTTGGTTAA‑CCAACTCCCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CC‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcgcgc < 2:774167/238‑1 (MQ=255) gcggcaggcttttttgtttgttAA‑CCAAACTCCGGAAGAACTGAACGAGTCCGCG‑CGTCCGAAAAAAA‑CT‑CTTGTGGGAGTCGATCTTAACC‑TGGCGCAGAAAGTTTTAGTTGGGC‑CGTAAACTTTGGTG‑GAAAAAAAC‑GCACCTGCCGATTTAGACCAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTaa < 1:180424/219‑1 (MQ=255) gggCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGACCTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTAGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCGGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAt < 2:566816/239‑1 (MQ=255) ggCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCCG‑CGTCGCGCGAAAA‑CA‑CTGCTGGCGGTAAAGATTAAAA‑TTGCGCAGAAAAATTTTGCTGGGC‑CGCAAAAACTTCTG‑GCGAAAATT‑CAACCTGCCCGTTTAGAACAAAAAC‑AACCAACGCGTTACTGGCAAGCAAAAAACGATTCCCAACCCAGGGGGCCCTTCCAtttttttcttcgtccggtaatt > 1:506773/1‑219 (MQ=255) tggtttgtaaccccaCTCCCGCAAGAACGGAACGGTGCCCGG‑CGTGGGGAAGAAA‑CA‑CTGATGGGGGGAGAGTTGAAAC‑GGGCGCAAAAAGATTTTGCTGGCG‑CGAAAAACTTGCGG‑GGGAAAAGC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAAcc < 1:351362/225‑1 (MQ=255) aaCTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg < 2:716029/205‑1 (MQ=255) aaCTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTgcg > 1:716029/1‑205 (MQ=255) cccccGCAAGAGATGAACATGGCTCGG‑GGGCGCGAGAAAA‑CC‑CCGCGGGCGGTAGAGTTAAAAC‑TGCCGCAGAAAGATTTTGCGGGCG‑CGCAAAACTTGCTG‑GAGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCgaa < 1:75633/236‑1 (MQ=255) aaaaCTGAACGATGCCCAG‑CGTCGGGAGAAAA‑CC‑TGGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCGG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGaaaaa < 2:447540/236‑1 (MQ=255) aaaCTGAACGTGCCTCAG‑CGT‑GCGAAAAAACCC‑CGGCGGGCGGTAAAGATTAACC‑TGGCGCAAAAAGTTTTTGCTGGCC‑CGCAAAACTTGCTG‑GCGAAAAGC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAca < 1:89338/238‑1 (MQ=255) aactaaaggagtgtccg‑CGTCGCAAAAAAA‑CC‑GGGCTGGCGGGAAGGATTAAAC‑TGGCGCAGAAAGATTTTGCGGGCG‑CGCAAAACTGGCTG‑GTAAAAATC‑CCACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGGCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAAcag < 1:573144/223‑1 (MQ=255) ccaggggcgagagaAAC‑CC‑TTGCGGGCGGTAGAGATTAAAC‑TGGCGCAAAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGAt < 2:886240/228‑1 (MQ=255) cAG‑CGTCGCGAGAAAA‑CC‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGAAAACCTGGCTG‑GGGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGc < 2:89285/239‑1 (MQ=255) ggTCGCGAGAAAA‑CC‑CGGCGGGCGGTAGAGATTAAAC‑TGGCGGAGAAAGATTTTGCGGGCG‑CGAAAACCTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCa < 1:584370/162‑1 (MQ=255) cgcgAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAAATGCTCAGGAACCGCTGCTTGGCGCGCAAGAAAAAAAGAAGAatt > 2:532991/1‑225 (MQ=255) gaAAA‑CC‑CTGCTGGCGGTAGAGTATAAAC‑TGGCCCAGAAAGATTTGTCTGGCG‑CCCAAAACTTGCTG‑GCGAAAACC‑CCACCTGCCGATTTAGAGCAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAg < 2:781085/238‑1 (MQ=255) gaAAA‑CC‑CTGCGGGCGTGTGATATTAAAC‑TGGCGAAGAAAGATTTTGCTGGCG‑CGAAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAg < 1:137933/238‑1 (MQ=255) aaa‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGGAAAAATCGA‑TGCCAGCCAGGGGCGGCCTTCCATTGATTTACt > 2:584370/1‑163 (MQ=255) aa‑CC‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGgcgc < 2:763244/239‑1 (MQ=255) a‑ca‑cTGCTGGCGGTAGAGATTAAAC‑TGGCGAAGAAAGATTTTGCCGGCC‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGct < 2:707558/239‑1 (MQ=255) ccctgctgGCGGTAGAAATTAAAC‑TGGCGCAGAAAGATTTGTCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGctc < 2:857912/237‑1 (MQ=255) tgctgGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCCAAAATCGA‑TGCCACCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCCACGGGACCGCGCCTTGTGCCGGACAAAAAACCACAGAAAATTGTTGCCACCCGGccagggccccc > 1:309136/1‑228 (MQ=255) ctgGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACCGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGGAAGAAAAACAGCAAAATATTGATGCCACCTGGCAGGCGCTcccct > 2:266227/1‑238 (MQ=255) tgGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCGGCTTGGCGCGAAAGAAAAACAGCCAGATATTGATGCCACCTGGCAGGCGCCCtccccc > 2:359972/1‑239 (MQ=255) gggttAGAGATTAAAC‑TGGCGAAAAAAGTTTTTGCTGGCG‑CGAAAAATTTGCTG‑GGGAAAACA‑CCCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt < 1:266227/235‑1 (MQ=255) ggttAGAGATTAAAC‑TGGCGAAGAAAGATTTTGCTGGCG‑CGCAAAGCTTGCTG‑GCGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt < 2:636199/235‑1 (MQ=255) cGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAt < 2:116256/238‑1 (MQ=255) gTAGAGTTTAAAC‑GGGCGCAGAAAGATTTGGCTGGCC‑CGCAAACCTTCATG‑GCGAAAATC‑ACCCCTGCCGATTTAAAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGa < 2:876308/238‑1 (MQ=255) aagagATTAAAC‑TGGCGAAGAAAGATTTTGCTGGCG‑GGAAAAACTTGCGG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGAc < 2:506773/237‑1 (MQ=255) ttAAAC‑GGGCGCAGAAAGATTTGGCTGGCG‑CGAAAAACTTGCTG‑GCCAAAATC‑ACCACGTCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAgg < 2:825350/237‑1 (MQ=255) aC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTTATTTACTGCGCGCGTTAATTTCCCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCAtaaacaaagaacaag > 1:281651/1‑225 (MQ=255) acgggagcAAAAAGA‑TTTGCTGGAG‑AGAAAACCTTGCTG‑GCGAAAATC‑ACCCATGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGGCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGc < 2:9137/233‑1 (MQ=255) gccggaaaacttttGCTGGAG‑CACAAACCGTGCTG‑GCAAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCTTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa < 2:186299/228‑1 (MQ=255) cgcAAAAAGATTTTGCTGGCG‑CGAAAAACTTGCGG‑GCAAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGaa < 2:644844/238‑1 (MQ=255) gAAAGTTTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACg < 2:594326/238‑1 (MQ=255) tGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGAATCAGGAACAGGCGACTAAGCTGGGGTTCa > 2:831162/1‑239 (MQ=255) tGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGAAGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCCGGGGATCa > 2:14414/1‑239 (MQ=255) gggCG‑CCGAAAACTTGCTG‑GCAAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACg < 2:721472/238‑1 (MQ=255) cg‑cgcAAAACTTGCTG‑GCGAAAATC‑CCCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGcc < 2:743806/238‑1 (MQ=255) cg‑cgaAAATCTTGCGG‑GGAAAAACC‑CCCCCTGCCGATTTAAAAAAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAAGCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGcc < 1:843835/238‑1 (MQ=255) cTTGCTG‑GCGAAAATC‑CCCCCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAata < 2:224150/239‑1 (MQ=255) gCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTTATGCCACCTGGCAGGCGCTCCCCCCCATGACTCAGGAAAAGGGGGAAAACCGGGGGATAAAAGCCGACCaaaaaaatt > 1:644498/1‑234 (MQ=255) | AGGCTGATTCTGCCTTTTATCTTCAGCAGATGCAGCAAAGCTCTGATGATACCAGGATCAACTGGCAATTACTCGCCATTCGTGCACTGGTGAAAGAAGGTAAAACCGGGCAGGCGGTTGAGTTGTTTAA‑CCAACTACCGCAAGAACTGAACGATGCTCAG‑CGTCGCGAGAAAA‑CA‑CTGCTGGCGGTAGAGATTAAAC‑TGGCGCAGAAAGATTTTGCTGGCG‑CGCAAAACTTGCTG‑GCGAAAATC‑ACACCTGCCGATTTAGAACAAAACC‑AGCAAGCGCGTTACTGGCAGGCAAAAATCGA‑TGCCAGCCAGGGGCGTCCTTCCATTGATTTACTGCGCGCGTTAATTGCTCAGGAACCGCTGCTTGGCGCGAAAGAAAAACAGCAGAATATTGATGCCACCTGGCAGGCGCTCTCCTCCATGACTCAGGAACAGGCGAATACGCTGGTGATCAACGCCGACGAAAATATTC > NC_000913/3293528‑3293998 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |