BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,373,356	C→T	11.5%	K73K (AAG→AAA)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,373,356	0	C	T	11.5%	247.0 / 2.6	109	K73K (AAG→AAA)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base C (27/64); new base T (11/3); total (42/67)
Fisher's exact test for biased strand distribution p-value = 6.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 109 total aligned reads displayed.

ATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATG  >  NC_000913/3373125‑3373593

atcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCACGTTGTTGGCTTTCGGCGGGGCTGACGCAACCGC‑CGTGGGCGGTGA‑G‑GTTA‑ATCTTACCtt                                                                                                                                                                                                                                              >  2:626904/1‑239 (MQ=255)

atcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGGTGCGACAACCCA‑CGGGGGGGAGGG‑G‑TTTA‑AATTAACCtt                                                                                                                                                                                                                                              >  1:488698/1‑239 (MQ=255)

      ggTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGTACCCAACCCG‑CGGGGGGGGAGG‑G‑TTTA‑ATTTTACCttttccc                                                                                                                                                                                                                                         >  1:69700/1‑234 (MQ=255)

          aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCCTCGAAGCCATAACGATTAGCCGATGCCGCAGGTTGTTGGCTTTCGGCGGTGCTGTGCCCACCGA‑CGTGGGCGATGC‑G‑TTTA‑ATTTTCACTTTTG‑CCTCTTc                                                                                                                                                                                                                                   >  1:95398/1‑239 (MQ=255)

          aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAGGTTGTTGGCTTTCGGCGGGGCGTACGCAACCGA‑CCGGGGCGATGG‑G‑TTTA‑ATTTTTCCTTTCG‑cgcctcc                                                                                                                                                                                                                                   >  1:586864/1‑233 (MQ=255)

          aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGGCGCAACCCA‑CGTGGGCGATGA‑G‑GTTA‑ATTTTACCTTTGC‑CCTCTTc                                                                                                                                                                                                                                   >  1:207950/1‑239 (MQ=255)

           gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAACGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGAGGCACACCA‑CACGGGCGCAGG‑G‑GTTT‑ATATTTCCTTTCT‑CCCCTTCg                                                                                                                                                                                                                                  >  1:677546/1‑239 (MQ=255)

           gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCg                                                                                                                                                                                                                                  >  2:714240/1‑239 (MQ=255)

           gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTGAGTCAACCAC‑CTGGGGCGATGA‑G‑GTTA‑ATCTTTACTTTCG‑CCCTTTCg                                                                                                                                                                                                                                  >  2:472201/1‑239 (MQ=255)

                  cccccctggtggtgaaaTGTTTAACCCCACCATCGGCAAACCAGCCGCAGAATTAATAATTGCCCGAGTTTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGATAC‑GGCAACTAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGAGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGcg‑acga                                                                                                                                                                                                                          <  1:564948/224‑1 (MQ=255)

                      cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑TGCATCGAAGCCATAACGTTTATCCGCTGCCGCAAGATGTTGGCTTTCGGCGGGGCGTGCGCCACCAA‑CGATGGCGATTAGG‑TTTA‑ACGTTACC‑TTCG‑CCTCTTCCGCG‑GCGG‑ttt                                                                                                                                                                                                                      >  1:526028/1‑238 (MQ=255)

                      cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGTCGGTGCTGACGCAACCGC‑CGTGGGCGAGGA‑G‑TTTA‑ATCTTACCTTTTG‑CCTCTTCTGGG‑CCGA‑ttt                                                                                                                                                                                                                      >  2:86027/1‑238 (MQ=255)

                      cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGCCGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGGG‑ACGA‑ttt                                                                                                                                                                                                                      >  2:546053/1‑238 (MQ=255)

                      cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACCGTGTGC‑ATGG‑G‑TTTA‑AACTTACCTTTTC‑CCCCTTCGGCG‑GCGA‑ttt                                                                                                                                                                                                                      >  2:24793/1‑238 (MQ=255)

                      cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CCTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑ttt                                                                                                                                                                                                                      >  2:466058/1‑238 (MQ=255)

                       cccggtcttgattgttttCCACCCCCAGCGGACAACCACCCTCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCGTCAAAGCGGAAAGGATAGTAGAACGGCGTGACGGCGGAGCC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑cc                                                                                                                                                                                                                   <  1:679919/227‑1 (MQ=255)

                           ggCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGATTCAATAATTGCCCGCTAGTGATCGCAGTGTTCTTCAAAGCTGATAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GACATCGAAGCCCTAACCTTTAGCCGCTGCCGCAAGTTTTTGGCTTTCGGCGGCGCAAACGACACCGA‑CATGGGGGATGT‑G‑GTAA‑ATATTTCCTTTCG‑CCTCTTCGGCG‑AGGA‑GTT‑cacaaa                                                                                                                                                                                                               >  1:630886/1‑234 (MQ=255)

                             cTGGAATGTTGTCACCCACCGTCGGCAAACCATCGCCCGAATCAATATTTGCCCGATCGGGATCGGAGTGTTCTTCAAAGCTGAAAGGATAGTAAAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTTTTGGCTTGCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTAc                                                                                                                                                                                                              <  2:224000/238‑1 (MQ=255)

                              tGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGCCGCAACCCC‑CGTGGGGGAGGA‑G‑TTTA‑ATTTTACCTTTTG‑CCCCTTCGGGC‑ACGA‑ttttccagaccc                                                                                                                                                                                                             >  1:181298/1‑230 (MQ=255)

                                gAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTGACGCAACCGC‑CGTGGGCGATGG‑A‑TTTA‑ATTTTACCTTTCT‑CCTCTTCGGCG‑ACGA‑TTT‑cacgaaccttt                                                                                                                                                                                                          >  2:18837/1‑229 (MQ=255)

                                  attttttcctcccccaatgcaaaaCCACCCCCCGAAACAATAATTGCCCGAAAGGGATCGGAGTGTTCTTAAAAGCTGAAAGGAAAGGAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGtt                                                                                                                                                                                                         <  1:291987/217‑1 (MQ=255)

                                     ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGTCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGGTTCGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGGTGCACCAACCGA‑CAAGGGGGATCG‑G‑TGTACACTTTACCTTTC‑‑CCACTTCGGCG‑CCCA‑TTT‑TCACTaaccggtagc                                                                                                                                                                                                      >  1:597620/1‑229 (MQ=255)

                                     ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACCCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTTAc                                                                                                                                                                                                      >  2:67502/1‑238 (MQ=255)

                                     ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTCGTGGCGGAACCCA‑CGTGGGGGATGA‑G‑GTTA‑ATTTTCCCTTTTGCCCTCTCCGGCG‑‑CGA‑TTT‑GCAGTGCCTGtttaa                                                                                                                                                                                                      >  1:743489/1‑235 (MQ=255)

                                            cccccaTGGGAAAACCCTCCCCCGAATCAATAATTGCCGCATAGTGATCGAAGGTTTTTTAAAACCTGAAAGGATAGTAGAACGGCGTGACGGGGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa                                                                                                                                                                                              <  1:804973/236‑1 (MQ=255)

                                            cccccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa                                                                                                                                                                                              <  2:744358/236‑1 (MQ=255)

                                            cccccaACGGCAAACCATCCGCCAAATCAAGAATTGCCCGATAGTGAACGCAGGGTTCTTCAAAGCTGAAAGGGTAGTAGAACGGCGGGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa                                                                                                                                                                                              <  2:834581/236‑1 (MQ=255)

                                             caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGGGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaaa                                                                                                                                                                                             >  2:65267/1‑239 (MQ=255)

                                             caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAATGGTTGGCTTTCGGCGGTGCTGAAGCCACCAC‑CGTGGGGGGTGA‑G‑TTTA‑ATCTTTCTTTTCC‑CCCTTTCGG‑G‑TCGAGTTT‑CCAAAACACGGTCACGCTCGGaaa                                                                                                                                                                                             >  1:375582/1‑239 (MQ=255)

                                                 ctccgCAAACCCACCCCCGAATAAATAATTGCCCGATAGTGATCGGAGTTTTCTTAAAAGGTGAAAGGATAGTAGAACGGCGGGCCGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCt                                                                                                                                                                                         <  1:714240/235‑1 (MQ=255)

                                                  tCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTTTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCtt                                                                                                                                                                                        <  2:444327/239‑1 (MQ=255)

                                                  tCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCtt                                                                                                                                                                                        <  2:69700/239‑1 (MQ=255)

                                                    ggCAACCCATCCGCCGAATTAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCt                                                                                                                                                                                         <  2:692165/236‑1 (MQ=255)

                                                         aCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑G‑GTTA‑ATCTTTCCTTTCG‑CCTCTTCGGCG‑AAGC‑TTT‑CCAGTACCCGTTTACGCCCGGAAAGGCTTTTT‑TCaa                                                                                                                                                                                >  2:92589/1‑239 (MQ=255)

                                                                 ccgAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCg                                                                                                                                                                        <  2:207950/239‑1 (MQ=255)

                                                                  caaataaataTTTGCCCGTTAGTTATGGCAGTTTTTTTCAAGGGTGAAAGGATGGTAGCAGGGCGGGACGGGGGGGCA‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGc                                                                                                                                                                       <  1:18837/237‑1 (MQ=255)

                                                                    aaTCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTTACGCCACCCA‑CGTGGGCCGTGG‑G‑TTTA‑ATCTTACCTTTTG‑CCTCCTCCGCG‑CCGG‑TTTCCCAGAACCT‑CTAACGCCCGCAAAGGCTTTTT‑CCAAAAGcccccccc                                                                                                                                                                     >  1:100020/1‑233 (MQ=255)

                                                                             ttGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAacca                                                                                                                                                            <  2:607518/239‑1 (MQ=255)

                                                                               ggccgggtgggagcgcgtgtttcatcAAAGCTGAAAGGAGATGAGACCGGGGGGACGGGGGAGAC‑GGCATCGAAGCGATAACGTATAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCGTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAaccacc                                                                                                                                                          <  1:5761/219‑1 (MQ=255)

                                                                               gCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGC‑CGTGGGCGATGG‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCCGTACCTGTTCACGCCCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAaccac                                                                                                                                                           >  2:673047/1‑238 (MQ=255)

                                                                               gCCCGATAGTGATCGCAGGTTTCTTAAAAGCGGAAAGGTTAGTAGAACGGGGTGACGGCGGAGAC‑GGGATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAaccacc                                                                                                                                                          <  2:612504/239‑1 (MQ=255)

                                                                               gCCCGATAGTGAGCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAaccacc                                                                                                                                                          <  2:208906/239‑1 (MQ=255)

                                                                                cccGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCCA‑CGGGGGCGATGA‑G‑GTTA‑ATCTTCCCTTTCG‑CCCCTTCGCCG‑CCGA‑TTT‑CCCGTACCCTTTCAACCCCGGAAAAGCTTTTG‑AAAAAGGCCTCCCCCGGGGacccaccc                                                                                                                                                         >  1:56058/1‑239 (MQ=255)

                                                                                 gacaggtggaccGCAGGGTTCTTAAAAGTTAAAAGGAGAGTAGAAGGGGGTGACGGGGGAGAC‑GGCATGGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAaccacc                                                                                                                                                          <  2:320836/226‑1 (MQ=255)

                                                                                    aTAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGGATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGG‑CGGGGGCGAAGA‑GTTTTA‑ATTTTCACTTT‑G‑CCTCTGCGGGGCACG‑‑TTT‑TCAGTACCAGTTTACCCCCCGAAAGGCATTTT‑CAAAAGGACTCACCggcgagacaccccccca                                                                                                                                                     >  1:434053/1‑224 (MQ=255)

                                                                                    aTAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTTACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCCCCGGTCGAACCACCCCCgc                                                                                                                                                     >  2:145886/1‑238 (MQ=255)

                                                                                         gATCGGAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑aaa                                                                                                                                               <  2:885530/239‑1 (MQ=255)

                                                                                          gcgcggtggtTTTTCAAAGCTAAAAGGTTAGTAGAACGGCGGGGCGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAAc                                                                                                                                              <  1:92589/231‑1 (MQ=255)

                                                                                          accGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑aaa                                                                                                                                               <  2:686138/236‑1 (MQ=255)

                                                                                                ggttttttaaaagggaaaaGGATTGTAAACGGGGGGGGCGGCGG‑GACGGGCATGGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCg                                                                                                                                         <  1:351751/222‑1 (MQ=255)

                                                                                                  gttttccAAAGAGGAAAGGTTATTAGGACGGGGTGGCGGGGGAGAC‑GGCACCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATg                                                                                                                                      <  1:181611/233‑1 (MQ=255)

                                                                                                   ttcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCt                                                                                                                                                                                                                                                    >  2:644338/1‑134 (MQ=255)

                                                                                                   ttcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCt                                                                                                                                                                                                                                                    <  1:644338/134‑1 (MQ=255)

                                                                                                    tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGCCGGCGGAGAC‑GGCCTCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGcc                                                                                                                                    <  2:488698/239‑1 (MQ=255)

                                                                                                    tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGGGGAGAC‑GGCAGCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGcc                                                                                                                                    <  2:518501/239‑1 (MQ=255)

                                                                                                      ttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGGGGGCGGTGG‑G‑TTTA‑ATCTTTACCTTCG‑CCCCTTCGGCC‑ACGA‑TTT‑CCAGTACCCGTTCAAGCCCCGGAAGGGTTTGTCACAAA‑GCCCCGCCCGGCGAAacaccccccaaaaaaaccccgaatgccg                                                                                                                                   >  1:814534/1‑217 (MQ=255)

                                                                                                        cAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTT‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGGCGAACCACCCCCGTAT‑AAACCGCCGATGCCctgc                                                                                                                                >  2:522729/1‑239 (MQ=255)

                                                                                                        aaaaGCGGAAAGGATATTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgc                                                                                                                                <  2:56058/238‑1 (MQ=255)

                                                                                                         aaaGGTTAAAGGATGAGAAAAGGGCTTGTCGGAGGAAGC‑GGCCGCGAAGCCATAACGGTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGCCCCAACCGA‑TGTTGGCGGTGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  1:595882/239‑1 (MQ=255)

                                                                                                         aaaGCTGAAAGGATTGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  2:586864/239‑1 (MQ=255)

                                                                                                         aaaGCTGAAAGGATAGTAGAACGGGGTGACGGCGGGGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  2:396617/239‑1 (MQ=255)

                                                                                                         aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  2:887813/239‑1 (MQ=255)

                                                                                                         aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  2:596406/239‑1 (MQ=255)

                                                                                                         aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCctgct                                                                                                                               <  2:544866/239‑1 (MQ=255)

                                                                                                           aGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGGAGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGGTCTCGCTCGGAAAGGCCTTTT‑TCAAAAGCCCCCCCGGTCGACACACCCCCGGAA‑AAAACCCCGATGCCCCGCTGa                                                                                                                             >  1:483000/1‑239 (MQ=255)

                                                                                                                       agtagAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGGGATGA‑G‑TTTA‑ATCTTCCCTTTCG‑CCTCTTCCCCG‑AGGA‑TTT‑CCCGTACCTGTTCCCGCCCCGGAAGGGTTTTT‑TCAAAGGCCCCCCCCGGCGAACCCCCCCCCCAA‑AAACCGTCCAAGCCCCTCTGaaaaataaccaaa                                                                                                                 >  1:610319/1‑228 (MQ=255)

                                                                                                                                 cGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑G‑TTTA‑ATCTTACCTTTTG‑CCTCTTCGGGG‑CCCG‑TTT‑CCAGTACCTGTTCCCGCTCGGGAAGGCTTTTT‑ACAAAGGACTCGCCGGTCGGACCCACCCCGTAT‑AAACCGCCGAGGCCCTGCCGAAAATTGAACAAAAACAGGGGAc                                                                                                       >  1:234979/1‑239 (MQ=255)

                                                                                                                                   tGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGCTGA‑G‑TTTA‑ATATTACCTTTGG‑CCTCTTCCGCG‑ACTG‑TTT‑TCACTACCTGTTCACCCTCGGGAAGGCTTTGT‑ACAAAAGCCCCGCCGGGCTCA‑CACCCCCGCATAAAACCCCTGATGCCCTTCTGAAAATtgaatgtaacaaaggcacaa                                                                                                     >  1:713551/1‑223 (MQ=255)

                                                                                                                                   tGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACg                                                                                                      <  2:69779/238‑1 (MQ=255)

                                                                                                                                       ggcggAGCC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGAc                                                                                                 <  2:332560/239‑1 (MQ=255)

                                                                                                                                        gggggggggggtgtCGAGGCAATGACGGTGAGGCGATGCCGGAAGTTGTTGGGCTCCGGCGGTCCGGGCGCAACCGA‑CGGGGGCGAGGA‑G‑TGTA‑ATCGTACCTTGCG‑CCTCGTCGGCG‑AGGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACGACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGAc                                                                                                 <  1:545231/226‑1 (MQ=255)

                                                                                                                                        gggggggacgcgttggaggtcttAATGTTTAGAGCATGCCGCAAGTTGTTGGCGTTCGGCGGTGCTGGCGCAACCGC‑AGTGGGCGAGTA‑G‑TTGA‑AGCTTACCGTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGATCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCACTGCTGAATATTGAACTGAACGAGACGACGCAGAc                                                                                                 <  1:273625/217‑1 (MQ=255)

                                                                                                                                        gcggGGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGAc                                                                                                 <  2:640743/238‑1 (MQ=255)

                                                                                                                                             gaC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCt                                                                                            <  2:181298/238‑1 (MQ=255)

                                                                                                                                             gaC‑GGCATCGAAGCCATAACGTTGAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCt                                                                                            <  2:526028/238‑1 (MQ=255)

                                                                                                                                                    aTCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTTTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCa                                                                                     <  2:787057/239‑1 (MQ=255)

                                                                                                                                                    aTCGAACCCATAACTTTTAGCCGGTGCCGCAAGTTTTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCa                                                                                     <  2:674619/239‑1 (MQ=255)

                                                                                                                                                               aaCGTTTCGCTGAGGCCGTAATTTGTTGGGTTTCGGCGGTGATGGCGTAACCGA‑CGGTGGCGATGA‑G‑TTTA‑ATCTTAACTTTCG‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTACATGTTCACGCTTGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCttgttgt                                                                          <  1:323486/239‑1 (MQ=255)

                                                                                                                                                               aaCGTTTAGCCGATGCCGCAAGTTGTTGGTTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTGCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCttgttgt                                                                          <  2:24223/239‑1 (MQ=255)

                                                                                                                                                                      gggcGATTCCGCAGGTTGTGGGCTTTGGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTTCCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCaa                                                                   <  1:466058/236‑1 (MQ=255)

                                                                                                                                                                        cctgtGCCGCAAGTTTTTGGCTTTCGGGGGTTCTGCCGCAACCGG‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTGCCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCaaaa                                                                 <  1:558242/235‑1 (MQ=255)

                                                                                                                                                                           aTGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTACTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGGGCTTGTTGTTGGTCAAAAGGa                                                              >  2:145069/1‑239 (MQ=255)

                                                                                                                                                                                gcggtttgttgGTTTTCGGGGGTGCTTGCGCAACCGC‑GGTGGGCGATTA‑G‑TTTA‑AGCTTACCTTTCG‑CTTCTTCGGCG‑CAGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGACAg                                                         <  1:185154/234‑1 (MQ=255)

                                                                                                                                                                                      ggttgGCTTCGGGCTGTGCTGGCGGAACCGA‑CGTGGGCGATGA‑G‑GTTA‑ATCCTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CAAGTCCCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGc                                                   <  2:434053/238‑1 (MQ=255)

                                                                                                                                                                                       gttgGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCa                                                  <  2:787815/239‑1 (MQ=255)

                                                                                                                                                                                         tgGCTTTCGGCGGTGCCGGCGCAAACGA‑CGGGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGAG‑GCGA‑GTT‑CCTGTACCCGTTCAACCTCGGACAGGCGTTGT‑TCCAAGGCCTCGCCGGTCGCCACACCCACGTAT‑ACAACGACGGTGCCCTCCTGCATATTGAACTCGACCCGGCGACGCAGAATCGCCTGATCCCCGCCTCGTTGTTGGACAAAAAGaagccggggtgaacc                                                >  1:118063/1‑225 (MQ=255)

                                                                                                                                                                                                cgggggTGTTGGCGCACCCGG‑TGGGGGCGGTGA‑G‑TTTA‑ACCTTTCCTTTCG‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTACCCGTTCCCGCCCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACg                                         <  1:145886/239‑1 (MQ=255)

                                                                                                                                                                                                cggcggTGCCGACCCAACCGG‑GCGGGGGGTTTG‑G‑TTTA‑ACCTTACCTTTCG‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTTCCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACg                                         <  1:170807/239‑1 (MQ=255)

                                                                                                                                                                                                             ccacccGA‑CGGGGGCGGTGG‑A‑TTTA‑ATTTTCCCTTTCG‑CCCTTTCGGGG‑GCGA‑TTC‑CCAGTACCTGTTCACGCCCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCg                            <  1:67502/235‑1 (MQ=255)

                                                                                                                                                                                                               aaCCGA‑CGTGGGCGTTTG‑T‑TTTA‑ACCTTTCCTTTCC‑CCTCTTCGGCG‑CCGA‑TTC‑CCGGACCCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGtt                          <  1:65267/239‑1 (MQ=255)

                                                                                                                                                                                                                         tggCGATGA‑G‑TTTA‑ATCTTGCCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CGAGTACCTATTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCGCGGCGGTCGAACCACCCACGTAT‑AAACCGTCGATGGCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGCCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  2:746759/237‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGTTGT‑G‑TTTT‑ATTCTACCTTTCG‑CCTCTTCGGGG‑ACGA‑GTT‑CCCTGTCCGGTTCCCCGCGGGAAAGGCTTTCT‑ACAAGGGCCTCGCCGGGCGAACCACCCCCGTAT‑AAACCGGCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCTACTACGCCACGTAAATTCGTTGCCATaaa                  <  1:142757/238‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGATTA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCC‑ACGA‑TTT‑CCAGTCCCTGTTCCCGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  2:814534/238‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGATGG‑T‑TTTA‑ATTTTTCCTTTCG‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCCCGCCCGGAAAAGCTTTTT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  1:626904/238‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGATGA‑T‑TTTA‑ATCTTTCCTTTCG‑CCTCTTCGGCG‑TCGA‑TTT‑CCAGTCCCTTTTCACGATCGGAACGGCTTTGT‑CCAAAGGCCTCGACGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  2:489400/238‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  2:483000/238‑1 (MQ=255)

                                                                                                                                                                                                                         gggCGATGA‑G‑TTTA‑ATCTTACCTTGCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa                  <  2:637991/238‑1 (MQ=255)

                                                                                                                                                                                                                          ggCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGcc                                                                                                                                    >  2:454709/1‑123 (MQ=255)

                                                                                                                                                                                                                          ggCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGcc                                                                                                                                    <  1:454709/123‑1 (MQ=255)

                                                                                                                                                                                                                               ttgtg‑TTTA‑ATCTTTCCTTTCC‑CCTTTTCGGCG‑ACGA‑TTT‑CCAGTTCCGGTTCCCGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACct             <  1:168764/234‑1 (MQ=255)

ATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGAC‑GGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑G‑TTTA‑ATCTTACCTTTCG‑CCTCTTCGGCG‑ACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGT‑ACAAAGGCCTCGCCGGTCGAACCACCCACGTAT‑AAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAATACCTCTGAAGTGATG  >  NC_000913/3373125‑3373593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`