Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,383,730 | A→C | 17.1% | G179G (GGT→GGG) | mdh ← | malate dehydrogenase, NAD(P)‑binding |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,383,730 | 0 | A | C | 17.1% | 117.5 / 3.3 | 68 | G179G (GGT→GGG) | mdh | malate dehydrogenase, NAD(P)‑binding |
Reads supporting (aligned to +/- strand): ref base A (34/19); new base C (2/12); total (36/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.76e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAG > NC_000913/3383495‑3383966 | cgtcccgcgcaccGTGGGCACATTCGACACCCGCTTGTGCTCCCTGCTGTGACCGACCCAGAG‑CAAACGCAGAACTGGCAGTGGCCTGGCCCACAGACAGGGTCGCGGACCCGCCTCCGG‑CCTTCGC‑TTCAACCTCTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑a < 1:631870/227‑1 (MQ=255) cGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑C‑AAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCCCTTCAGTACCCGCGTTCTGGATGCGGTTGTCCAGATCAGCCACTTCCTTCTCGGTAAAACTAACCCCCGGAACCTTTGACAGGCGCGGCAGAA‑TGGGAACCCC‑aa > 1:538108/1‑238 (MQ=255) cGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CCAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTAGC‑TTCAACCACTTCAGCACACGCGTTCTGGATGCGTTTGGTCGAATCAGACACGTCATGCTCGGAAAAACCTACGCAAGGAACCTGTACAACCAGGGGACAAA‑AGGGAACACA‑AGAGTGAcacaca > 1:247659/1‑234 (MQ=255) aGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATCCGTTTGGTCAGATCAGCCACTTCTTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCATCGGCAGAA‑TGCAAACACC‑AAAGGGACCGCCATTa > 2:474515/1‑239 (MQ=255) ggCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCCGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGACCCCTGGACATCAACGGAGGAA‑AGGTAAAACA‑AAACGGAACGCCaaaa > 1:563101/1‑236 (MQ=255) cGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAAATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCGGTGCCACCAGCGGCAGAA‑TGGGAACCCC‑AGAAGGACCCCCAATAACAGGGAc > 1:259341/1‑238 (MQ=255) cGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACt > 2:326182/1‑239 (MQ=255) cGCCTTGTCCGCCCGGCAGTGCACGACCCAGAGACAAAC‑CAAAACGTGCAGCTGCCTGGCCCATAGCCGGGGTTGCAGCCCCGCCCCCGG‑CCTTCGC‑TTCACCCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACt < 2:588565/239‑1 (MQ=255) gCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATCCGTTTGGTCAGATCAGCCCCTTCCTGCTCGGTAAAACTAACCCCAGGAACCTGTGACAGCACGGCCAGAA‑TGGTACAACCAAGAG‑GACCGCCAATAACCgcaccttcacct > 1:838785/1‑227 (MQ=255) gTTCCCCCTGCGGTGCACGAACCAGAGACAGAC‑CAAACCGGGCAGTTGCCTGGCCAATAGAAGGGGTTGCAGCCCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCt < 2:3039/239‑1 (MQ=255) ccTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGGACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGt > 2:436808/1‑239 (MQ=255) ccTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGt > 2:398077/1‑239 (MQ=255) ccTGCAGTGCACGAACCAGAGACAGAC‑CAAAACATGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCTATTCAGTACCCGCGTTCTGGATGCGTTTACTCAGATCAGCCACTTCCTGCTCGGTCAAACTAACGCCAGGTACCGTCGACGACAGC‑GCAGAACTGGTAAAACA‑CGCGCCACCACCCCGAACCGAGACTTCAACTTCGCCTGGATGt > 1:78627/1‑239 (MQ=255) cACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTc > 2:216364/1‑239 (MQ=255) aCCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAACCTAACGCCAGGAACCTGTGACCGCAGCGCCAGAA‑TGGTAACACC‑AGATGGACCCCCAATAACCGGCCCTTCAACTTCGCCTGGCCGTTTGCCTTTCAGTTc > 1:585644/1‑239 (MQ=255) aCCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGAGACCGCCACTAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGtt > 2:612557/1‑238 (MQ=255) aCCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AAGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGtt > 2:159943/1‑238 (MQ=255) aCCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTCGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑AGGTAACACC‑AGAGTGACCGCCAAAAACCGGCCATTCAACTTCGCCAGGCTGTTTGCCTTTCAGGTc > 1:395551/1‑239 (MQ=255) caagc‑cAAACCGGGCCGCTGCCGGCCCCATAGCAGGGGTTGCAGCCCCGCCACCGG‑CCTTCGC‑TTCAACCACCTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGACCCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGcaacaa < 2:717150/235‑1 (MQ=255) gac‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGAGGCAGAA‑TGGTAAAACC‑AGAGTGACCGCCAATAACCGCCCCTTCAACTTCGCCTGGCTGGTTCCCTTTCAATTCCCGAAAAAAg > 1:403793/1‑239 (MQ=255) ac‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AAAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCTGCAACAAAgg > 2:658291/1‑239 (MQ=255) ac‑CAAAACGGGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCAACCGG‑CCTTCGC‑TTCAACCACTTCAGGACCCGAGTTCTGGATGCGTTTAGCCAGATCAGCCACTTCCTGCCCGGTAAAACTAACGCCCGGCACCTCTGTCGTCACCGCCCGAA‑TGGTACCACC‑AGACTGACCGCCAATAACCGGCCGTTCAACTTGGCCCTGATCTTTGCCTTTCAGTACCGAAACAAAga > 1:715395/1‑238 (MQ=255) gggcggtgctcgtcctatggACGGGGTTGAGGA‑CCGCCCCCGG‑GTGTCGCTTTCAACCCATCCAGCACCGGCGTGCGGGATGCGTTTGGTCAGAGCAGCCACTGCCGGCTCGGTAAACCTAACCCCAGGAACCGGTGACAGCAGCGGCAGAA‑TGGTAACCCC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTGTCAGTTCCGCAACAAAGGTGTTGGAAc < 1:62891/220‑1 (MQ=255) ggcggCTGCCTGGCCCAAAAACGGGGTTGCACCCCCGCCCCCGG‑CCTTCCC‑TTCAACCCCTTCAGTACCCGCGTTCTGGATGCGTTTGGCCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCCGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc < 1:812901/235‑1 (MQ=255) gcgcctcctgggcccaagaccggggTTGCAGGCCCCCCACCGG‑CCTTTCC‑TTCACCCCCTTCAGTCCCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc < 2:542010/216‑1 (MQ=255) gaccgtGCCGGCCCCATAGCCAGGGTTCAAGACCCCCCCCCGG‑CCTTCCC‑TTAAACCACTTAATTCCCCGCGTTCTGGATGCGTTGGGCCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc < 2:610159/233‑1 (MQ=255) cggCCTGCCCCATAGACGGGGTTGCAGACCCGCCCCCGG‑CCTTCGC‑TTCAACCCCTTCAGTACCCGGTTTCTGGATGCGTTTGGTCAGACCAGCCACTTCCTGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAAt < 2:623783/236‑1 (MQ=255) ccTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAAAAAAGGAGTTGGAACAAAAGaaa > 1:541570/1‑237 (MQ=255) agggtttgcgACCCCCCCCCGGCCCTTCCC‑TCAACCCCCTGCAGTCCCCGCGTTGGGGGTGCGTTTGGCCAGATCCGCCACTTCCGGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACCCC‑AGGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTa < 1:704003/229‑1 (MQ=255) gggTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑ACAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAAATCCAGCGTGGTAAc > 2:418185/1‑238 (MQ=255) aCCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAgt > 2:586815/1‑239 (MQ=255) ccctttCAGTCCCCGTGTTCTGGAGGCGTTTGGTCAGATCATCCTCTTCCTGCGCGGTAAAACTAACGCCAGGACCCTGTGACAGCGCCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCt < 1:578784/235‑1 (MQ=255) tcccgtgttttgtggctgtttttGTCACGTCCGCCCCTTCGTGCGCGTAAAAACTAGAGCCAGACACCGGGGAAGGCAGGGGAAAAA‑TGTGAACCCA‑AGGTTGTCCGCCAATAACCGGCCCTTCAACTTCACCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAg < 1:418185/219‑1 (MQ=255) tACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACCCCGAACAGTTTGTTTTTGTCATAAAAACCGACctttttaaac > 1:25602/1‑235 (MQ=255) gcgTTCTGGATGCGTTTGGTCAGATCATCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATACCCGGCCCTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCCACAAAGGTGATGGAACGAATGTTATCCAGCCGGGGAACGCCGAACAGTTTTTTTTTTTCATAAAAACCGGCTTTTTTACGCACTTc > 1:868824/1‑239 (MQ=255) gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACACAGGAGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTAGTTTTTGTCATAAACACCGGCTTTCTTCAGCCCTTc > 2:645213/1‑239 (MQ=255) gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTGTTTGTCATAAACACCGGCGTTTTTCAGCACTTc > 2:70818/1‑239 (MQ=255) gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGCCAGAA‑TGGTAACAAC‑AAAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTACCTTTCAGTCCCCAAACAAAGGTGTTGGAACGCATGACATCCACAGTGGTAACGCCGAACAGTTTGTTTTTGCGATAAACACCTCACTTTTTCATCACTTc > 1:62585/1‑239 (MQ=255) gTTCTGGATGCGTGTGGGCAGATCAGCCACTTCCTGCCCGGTAAAACTAACGCCAGGAACCGGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcag < 2:739240/239‑1 (MQ=255) tcgggATGGTTTGGGTCAGTTCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACCCC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcagca < 2:541570/236‑1 (MQ=255) cgggAGGCGTTTGTCCAGATCAGCCACTCCCTGATGGGTAAAACTACCGCAAGGAACCTGTGACAGCAGCGGCAAAA‑TGTGAACCCC‑AGAAGGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcagcag < 2:563101/237‑1 (MQ=255) tGGATGCGTTTGGGCAGACCCACCACTTCCTGCCCGGTAAAACTAACGCCAGGAACCTTTGACCGCAGCGGCAGAA‑TGGTAACCCC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCagcagc < 2:374022/239‑1 (MQ=255) tccctttGGTCAGATCAGCCCCTTCCTGCTCGGTAAACCTACCGCCAGGAACCTGTGAAAGCAGCGGCAGAA‑TGGTAACCCC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAAt < 2:577570/234‑1 (MQ=255) tGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACCCCGGCTTTTTTCAGCACTTCAGCAGCAATTGCa > 2:383974/1‑236 (MQ=255) tGGCCAGACCAGCACCTGCCTGCCCGGTAAAACTAACGCCAGGAACCGGGGACAGCAGGGGCAGAA‑GGGTAACCCC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACt < 2:259341/239‑1 (MQ=255) tgtcgggtcggCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAAAA‑GGGAAACACC‑GGAGTGACCGCCAATACCCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg < 2:402345/229‑1 (MQ=255) tgtccgtcccgCCACTTCCTGCACGGAAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCATAA‑TGGTACCCCC‑GGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg < 1:630573/229‑1 (MQ=255) ggTCAAATCAGCCCCTTCCGGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg < 2:395551/239‑1 (MQ=255) tCAGATCCGCCCCTTCCTGCGCGGTAAAACTAACGCCAGGAACCTGTTACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtgt < 2:316323/238‑1 (MQ=255) aTCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGgtgt > 2:419146/1‑239 (MQ=255) gCCCCTCCCTGCTCGGTAAAGCCAACGCCGGGACCCTGTGACGGACTCGGCAGAA‑TGGTACCGCC‑AGAGTGACCGACAATAACCGGCACTTTAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAAc < 2:796236/239‑1 (MQ=255) cACTTCCGGTTGGGAAAAACGAACGCAAGGAACCGGTGACACCGGCGGCAGAA‑GGGGAACACC‑AGAGGTGCCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAAc < 2:715395/237‑1 (MQ=255) tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGCATGATATCCAGCGTCGTAACGCCGGACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAACAGCAATTGCAACTGGGGTTGTAACCgggat > 1:823386/1‑237 (MQ=255) tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGtt > 2:196276/1‑239 (MQ=255) tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTAGACAGCAATTGCAACTGGGGTGTTAACCGGGtt > 2:830485/1‑239 (MQ=255) ccTGCACGGAAAAACTAACGCCAGAAACCTGTCACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTa < 2:762373/239‑1 (MQ=255) gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCCTAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGataat > 2:546404/1‑239 (MQ=255) gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCATCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGGGataat > 2:355237/1‑239 (MQ=255) gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg < 2:350284/214‑1 (MQ=255) gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGACACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGAAACtg > 1:350284/1‑214 (MQ=255) aCGCCAGGACCCTGCGACAGAGGCGGCAGAA‑TGGTACCACC‑GGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCa < 2:842230/239‑1 (MQ=255) ccaggaactgtcgccccccgcGGCAAAA‑TTGACACCCC‑AGAGTGACCGCCAATAACCGGCCCTTTAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGc < 1:398077/220‑1 (MQ=255) ggAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTTGCTGTGTGCCTTTCAGTTCCGAAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCCGCACTTCGGCAGCAATTACAACTTGGGGGTGAACGGGGTTAATGTGAATACCAATGCACGCtt > 1:864465/1‑238 (MQ=255) cctggaaCAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGCCCGCCAATAACCGGCACTTCAACCTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCggg < 2:495096/233‑1 (MQ=255) agcGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCCCTTCAGCAGCAATTGCAACTGTGGGGTTAACCGGGTTAGTGATAATCCAAATGCACGCTTTCGGGCAGGGTTTCga > 1:174196/1‑238 (MQ=255) ggCAGAA‑TGGAAACACC‑AGATTGACCGCCAATAACCGGCACTTCAAGTTCGCCTGGCTGGTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGGTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAGCTGGGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCa < 2:390848/237‑1 (MQ=255) gCAGAA‑GGGTAACCCC‑AGGGGTACCGACAATAACCGGCACTTCAACTGCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAAc < 2:382933/238‑1 (MQ=255) a‑TGGTACCCCC‑AGAGTGCCCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTg < 2:538108/236‑1 (MQ=255) acacC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAg > 2:103439/1‑239 (MQ=255) | CGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGAC‑CAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGG‑CCTTCGC‑TTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAA‑TGGTAACACC‑AGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAG > NC_000913/3383495‑3383966 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |