Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,383,730 A→C 17.1% G179G (GGT→GGG mdh ← malate dehydrogenase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,383,7300AC17.1% 117.5 / 3.3 68G179G (GGT→GGGmdhmalate dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (34/19);  new base C (2/12);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAG  >  NC_000913/3383495‑3383966
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
cgtcccgcgcaccGTGGGCACATTCGACACCCGCTTGTGCTCCCTGCTGTGACCGACCCAGAGCAAACGCAGAACTGGCAGTGGCCTGGCCCACAGACAGGGTCGCGGACCCGCCTCCGGCCTTCGCTTCAACCTCTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCa                                                                                                                                                                                                                                           <  1:631870/227‑1 (MQ=255)
cGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCCCTTCAGTACCCGCGTTCTGGATGCGGTTGTCCAGATCAGCCACTTCCTTCTCGGTAAAACTAACCCCCGGAACCTTTGACAGGCGCGGCAGAATGGGAACCCCaa                                                                                                                                                                                                                                          >  1:538108/1‑238 (MQ=255)
            cGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCCAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTAGCTTCAACCACTTCAGCACACGCGTTCTGGATGCGTTTGGTCGAATCAGACACGTCATGCTCGGAAAAACCTACGCAAGGAACCTGTACAACCAGGGGACAAAAGGGAACACAAGAGTGAcacaca                                                                                                                                                                                                                               >  1:247659/1‑234 (MQ=255)
               aGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATCCGTTTGGTCAGATCAGCCACTTCTTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCATCGGCAGAATGCAAACACCAAAGGGACCGCCATTa                                                                                                                                                                                                                            >  2:474515/1‑239 (MQ=255)
                ggCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCCGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGACCCCTGGACATCAACGGAGGAAAGGTAAAACAAAACGGAACGCCaaaa                                                                                                                                                                                                                            >  1:563101/1‑236 (MQ=255)
                        cGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAAATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCGGTGCCACCAGCGGCAGAATGGGAACCCCAGAAGGACCCCCAATAACAGGGAc                                                                                                                                                                                                                    >  1:259341/1‑238 (MQ=255)
                              cGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACt                                                                                                                                                                                                             >  2:326182/1‑239 (MQ=255)
                              cGCCTTGTCCGCCCGGCAGTGCACGACCCAGAGACAAACCAAAACGTGCAGCTGCCTGGCCCATAGCCGGGGTTGCAGCCCCGCCCCCGGCCTTCGCTTCACCCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACt                                                                                                                                                                                                             <  2:588565/239‑1 (MQ=255)
                               gCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATCCGTTTGGTCAGATCAGCCCCTTCCTGCTCGGTAAAACTAACCCCAGGAACCTGTGACAGCACGGCCAGAATGGTACAACCAAGAGGACCGCCAATAACCgcaccttcacct                                                                                                                                                                                                             >  1:838785/1‑227 (MQ=255)
                                    gTTCCCCCTGCGGTGCACGAACCAGAGACAGACCAAACCGGGCAGTTGCCTGGCCAATAGAAGGGGTTGCAGCCCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCt                                                                                                                                                                                                       <  2:3039/239‑1 (MQ=255)
                                          ccTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGGACCTGTGACAGCAGCGGCAGAATGGTAACACCAGGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGt                                                                                                                                                                                                 >  2:436808/1‑239 (MQ=255)
                                          ccTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGt                                                                                                                                                                                                 >  2:398077/1‑239 (MQ=255)
                                          ccTGCAGTGCACGAACCAGAGACAGACCAAAACATGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCTATTCAGTACCCGCGTTCTGGATGCGTTTACTCAGATCAGCCACTTCCTGCTCGGTCAAACTAACGCCAGGTACCGTCGACGACAGCGCAGAACTGGTAAAACACGCGCCACCACCCCGAACCGAGACTTCAACTTCGCCTGGATGt                                                                                                                                                                                                 >  1:78627/1‑239 (MQ=255)
                                                   cACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTc                                                                                                                                                                                        >  2:216364/1‑239 (MQ=255)
                                                        aCCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAACCTAACGCCAGGAACCTGTGACCGCAGCGCCAGAATGGTAACACCAGATGGACCCCCAATAACCGGCCCTTCAACTTCGCCTGGCCGTTTGCCTTTCAGTTc                                                                                                                                                                                   >  1:585644/1‑239 (MQ=255)
                                                        aCCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGAGACCGCCACTAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGtt                                                                                                                                                                                    >  2:612557/1‑238 (MQ=255)
                                                        aCCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAAGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGtt                                                                                                                                                                                    >  2:159943/1‑238 (MQ=255)
                                                        aCCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTCGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAAAGGTAACACCAGAGTGACCGCCAAAAACCGGCCATTCAACTTCGCCAGGCTGTTTGCCTTTCAGGTc                                                                                                                                                                                   >  1:395551/1‑239 (MQ=255)
                                                                caagccAAACCGGGCCGCTGCCGGCCCCATAGCAGGGGTTGCAGCCCCGCCACCGGCCTTCGCTTCAACCACCTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGACCCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGcaacaa                                                                                                                                                                           <  2:717150/235‑1 (MQ=255)
                                                                  gacCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGAGGCAGAATGGTAAAACCAGAGTGACCGCCAATAACCGCCCCTTCAACTTCGCCTGGCTGGTTCCCTTTCAATTCCCGAAAAAAg                                                                                                                                                                         >  1:403793/1‑239 (MQ=255)
                                                                   acCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAAAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCTGCAACAAAgg                                                                                                                                                                        >  2:658291/1‑239 (MQ=255)
                                                                   acCAAAACGGGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCAACCGGCCTTCGCTTCAACCACTTCAGGACCCGAGTTCTGGATGCGTTTAGCCAGATCAGCCACTTCCTGCCCGGTAAAACTAACGCCCGGCACCTCTGTCGTCACCGCCCGAATGGTACCACCAGACTGACCGCCAATAACCGGCCGTTCAACTTGGCCCTGATCTTTGCCTTTCAGTACCGAAACAAAga                                                                                                                                                                        >  1:715395/1‑238 (MQ=255)
                                                                             gggcggtgctcgtcctatggACGGGGTTGAGGACCGCCCCCGGGTGTCGCTTTCAACCCATCCAGCACCGGCGTGCGGGATGCGTTTGGTCAGAGCAGCCACTGCCGGCTCGGTAAACCTAACCCCAGGAACCGGTGACAGCAGCGGCAGAATGGTAACCCCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTGTCAGTTCCGCAACAAAGGTGTTGGAAc                                                                                                                                                               <  1:62891/220‑1 (MQ=255)
                                                                             ggcggCTGCCTGGCCCAAAAACGGGGTTGCACCCCCGCCCCCGGCCTTCCCTTCAACCCCTTCAGTACCCGCGTTCTGGATGCGTTTGGCCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCCGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc                                                                                                                                                               <  1:812901/235‑1 (MQ=255)
                                                                              gcgcctcctgggcccaagaccggggTTGCAGGCCCCCCACCGGCCTTTCCTTCACCCCCTTCAGTCCCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc                                                                                                                                                               <  2:542010/216‑1 (MQ=255)
                                                                              gaccgtGCCGGCCCCATAGCCAGGGTTCAAGACCCCCCCCCGGCCTTCCCTTAAACCACTTAATTCCCCGCGTTCTGGATGCGTTGGGCCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAAc                                                                                                                                                               <  2:610159/233‑1 (MQ=255)
                                                                                  cggCCTGCCCCATAGACGGGGTTGCAGACCCGCCCCCGGCCTTCGCTTCAACCCCTTCAGTACCCGGTTTCTGGATGCGTTTGGTCAGACCAGCCACTTCCTGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAAt                                                                                                                                                           <  2:623783/236‑1 (MQ=255)
                                                                                     ccTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAAAAAAGGAGTTGGAACAAAAGaaa                                                                                                                                                       >  1:541570/1‑237 (MQ=255)
                                                                                                   agggtttgcgACCCCCCCCCGGCCCTTCCCTCAACCCCCTGCAGTCCCCGCGTTGGGGGTGCGTTTGGCCAGATCCGCCACTTCCGGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACCCCAGGGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTa                                                                                                                                           <  1:704003/229‑1 (MQ=255)
                                                                                                    gggTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCACAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGAAATCCAGCGTGGTAAc                                                                                                                                         >  2:418185/1‑238 (MQ=255)
                                                                                                             aCCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAgt                                                                                                                               >  2:586815/1‑239 (MQ=255)
                                                                                                                                       ccctttCAGTCCCCGTGTTCTGGAGGCGTTTGGTCAGATCATCCTCTTCCTGCGCGGTAAAACTAACGCCAGGACCCTGTGACAGCGCCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCt                                                                                                       <  1:578784/235‑1 (MQ=255)
                                                                                                                                                tcccgtgttttgtggctgtttttGTCACGTCCGCCCCTTCGTGCGCGTAAAAACTAGAGCCAGACACCGGGGAAGGCAGGGGAAAAATGTGAACCCAAGGTTGTCCGCCAATAACCGGCCCTTCAACTTCACCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAg                                                                                               <  1:418185/219‑1 (MQ=255)
                                                                                                                                                tACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACCCCGAACAGTTTGTTTTTGTCATAAAAACCGACctttttaaac                                                                                              >  1:25602/1‑235 (MQ=255)
                                                                                                                                                     gcgTTCTGGATGCGTTTGGTCAGATCATCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATACCCGGCCCTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCCACAAAGGTGATGGAACGAATGTTATCCAGCCGGGGAACGCCGAACAGTTTTTTTTTTTCATAAAAACCGGCTTTTTTACGCACTTc                                                                                         >  1:868824/1‑239 (MQ=255)
                                                                                                                                                     gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACACAGGAGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTAGTTTTTGTCATAAACACCGGCTTTCTTCAGCCCTTc                                                                                         >  2:645213/1‑239 (MQ=255)
                                                                                                                                                     gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTGTTTGTCATAAACACCGGCGTTTTTCAGCACTTc                                                                                         >  2:70818/1‑239 (MQ=255)
                                                                                                                                                     gcgTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGCCAGAATGGTAACAACAAAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTACCTTTCAGTCCCCAAACAAAGGTGTTGGAACGCATGACATCCACAGTGGTAACGCCGAACAGTTTGTTTTTGCGATAAACACCTCACTTTTTCATCACTTc                                                                                         >  1:62585/1‑239 (MQ=255)
                                                                                                                                                       gTTCTGGATGCGTGTGGGCAGATCAGCCACTTCCTGCCCGGTAAAACTAACGCCAGGAACCGGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcag                                                                                       <  2:739240/239‑1 (MQ=255)
                                                                                                                                                         tcgggATGGTTTGGGTCAGTTCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACCCCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcagca                                                                                     <  2:541570/236‑1 (MQ=255)
                                                                                                                                                          cgggAGGCGTTTGTCCAGATCAGCCACTCCCTGATGGGTAAAACTACCGCAAGGAACCTGTGACAGCAGCGGCAAAATGTGAACCCCAGAAGGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTcagcag                                                                                    <  2:563101/237‑1 (MQ=255)
                                                                                                                                                           tGGATGCGTTTGGGCAGACCCACCACTTCCTGCCCGGTAAAACTAACGCCAGGAACCTTTGACCGCAGCGGCAGAATGGTAACCCCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCagcagc                                                                                   <  2:374022/239‑1 (MQ=255)
                                                                                                                                                               tccctttGGTCAGATCAGCCCCTTCCTGCTCGGTAAACCTACCGCCAGGAACCTGTGAAAGCAGCGGCAGAATGGTAACCCCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAAt                                                                                <  2:577570/234‑1 (MQ=255)
                                                                                                                                                                     tGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACCCCGGCTTTTTTCAGCACTTCAGCAGCAATTGCa                                                                            >  2:383974/1‑236 (MQ=255)
                                                                                                                                                                     tGGCCAGACCAGCACCTGCCTGCCCGGTAAAACTAACGCCAGGAACCGGGGACAGCAGGGGCAGAAGGGTAACCCCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACt                                                                         <  2:259341/239‑1 (MQ=255)
                                                                                                                                                                      tgtcgggtcggCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAAAAGGGAAACACCGGAGTGACCGCCAATACCCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg                                                                        <  2:402345/229‑1 (MQ=255)
                                                                                                                                                                      tgtccgtcccgCCACTTCCTGCACGGAAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCATAATGGTACCCCCGGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg                                                                        <  1:630573/229‑1 (MQ=255)
                                                                                                                                                                      ggTCAAATCAGCCCCTTCCGGCTCGGTAAACCTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg                                                                        <  2:395551/239‑1 (MQ=255)
                                                                                                                                                                        tCAGATCCGCCCCTTCCTGCGCGGTAAAACTAACGCCAGGAACCTGTTACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtgt                                                                       <  2:316323/238‑1 (MQ=255)
                                                                                                                                                                            aTCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGgtgt                                                                  >  2:419146/1‑239 (MQ=255)
                                                                                                                                                                                gCCCCTCCCTGCTCGGTAAAGCCAACGCCGGGACCCTGTGACGGACTCGGCAGAATGGTACCGCCAGAGTGACCGACAATAACCGGCACTTTAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAAc                                                              <  2:796236/239‑1 (MQ=255)
                                                                                                                                                                                  cACTTCCGGTTGGGAAAAACGAACGCAAGGAACCGGTGACACCGGCGGCAGAAGGGGAACACCAGAGGTGCCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAAc                                                              <  2:715395/237‑1 (MQ=255)
                                                                                                                                                                                      tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGCATGATATCCAGCGTCGTAACGCCGGACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAACAGCAATTGCAACTGGGGTTGTAACCgggat                                                        >  1:823386/1‑237 (MQ=255)
                                                                                                                                                                                      tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGtt                                                        >  2:196276/1‑239 (MQ=255)
                                                                                                                                                                                      tCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTAGACAGCAATTGCAACTGGGGTGTTAACCGGGtt                                                        >  2:830485/1‑239 (MQ=255)
                                                                                                                                                                                       ccTGCACGGAAAAACTAACGCCAGAAACCTGTCACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTa                                                       <  2:762373/239‑1 (MQ=255)
                                                                                                                                                                                               gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCCTAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGataat                                               >  2:546404/1‑239 (MQ=255)
                                                                                                                                                                                               gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCATCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGGGataat                                               >  2:355237/1‑239 (MQ=255)
                                                                                                                                                                                               gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACtg                                                                        <  2:350284/214‑1 (MQ=255)
                                                                                                                                                                                               gTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGACACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGAAACtg                                                                        >  1:350284/1‑214 (MQ=255)
                                                                                                                                                                                                        aCGCCAGGACCCTGCGACAGAGGCGGCAGAATGGTACCACCGGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCa                                      <  2:842230/239‑1 (MQ=255)
                                                                                                                                                                                                           ccaggaactgtcgccccccgcGGCAAAATTGACACCCCAGAGTGACCGCCAATAACCGGCCCTTTAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGc                                   <  1:398077/220‑1 (MQ=255)
                                                                                                                                                                                                              ggAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTTGCTGTGTGCCTTTCAGTTCCGAAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCCGCACTTCGGCAGCAATTACAACTTGGGGGTGAACGGGGTTAATGTGAATACCAATGCACGCtt                                 >  1:864465/1‑238 (MQ=255)
                                                                                                                                                                                                                  cctggaaCAGCAGCGGCAGAATGGTAACACCAGAGTGCCCGCCAATAACCGGCACTTCAACCTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCggg                            <  2:495096/233‑1 (MQ=255)
                                                                                                                                                                                                                             agcGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCCCTTCAGCAGCAATTGCAACTGTGGGGTTAACCGGGTTAGTGATAATCCAAATGCACGCTTTCGGGCAGGGTTTCga                 >  1:174196/1‑238 (MQ=255)
                                                                                                                                                                                                                                ggCAGAATGGAAACACCAGATTGACCGCCAATAACCGGCACTTCAAGTTCGCCTGGCTGGTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGGTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAGCTGGGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCa                <  2:390848/237‑1 (MQ=255)
                                                                                                                                                                                                                                 gCAGAAGGGTAACCCCAGGGGTACCGACAATAACCGGCACTTCAACTGCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAAc              <  2:382933/238‑1 (MQ=255)
                                                                                                                                                                                                                                      aTGGTACCCCCAGAGTGCCCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTg           <  2:538108/236‑1 (MQ=255)
                                                                                                                                                                                                                                             acacCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAg  >  2:103439/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             
CGTCGCCTTCAACGTAGGCACATTCGACAACGCCTTGTTCGCCCTGCAGTGCACGAACCAGAGACAGACCAAAACGTGCAGCTGCCTGGCCCATAGACAGGGTTGCAGACCCGCCACCGGCCTTCGCTTCAACCACTTCAGTACCCGCGTTCTGGATGCGTTTGGTCAGATCAGCCACTTCCTGCTCGGTAAAACTAACGCCAGGAACCTGTGACAGCAGCGGCAGAATGGTAACACCAGAGTGACCGCCAATAACCGGCACTTCAACTTCGCCTGGCTGTTTGCCTTTCAGTTCCGCAACAAAGGTGTTGGAACGAATGATATCCAGCGTGGTAACGCCGAACAGTTTGTTTTTGTCATAAACACCGGCTTTTTTCAGCACTTCAGCAGCAATTGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGCAACTTGCTGTACCAG  >  NC_000913/3383495‑3383966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: