| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,416,444 | A→T | 15.5% | I472F (ATC→TTC) | acrF → | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,416,444 | 0 | A | T | 15.5% | 146.3 / 2.4 | 66 | I472F (ATC→TTC) | acrF | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (9/43); new base T (9/2); total (21/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCT > NC_000913/3416214‑3416667 | tGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTAAGCGGTATTTATTCCGATGGCATCCTTCGGCGGTTCTCA‑TTGGGCCATTTTTCGCCCGTTTTCTTTCACCAt > 2:813032/1‑238 (MQ=255) ttCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGGGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TTGGGGCATTTTTCGCCAGTTCTCTTTCCCCCTCGt > 2:627305/1‑239 (MQ=255) ggTGCTTGCCATCGGGCTGCTCGTGGATGATGCGATTGTGGTGGTGGAGAACGTCGAGCGGGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCa < 2:515514/238‑1 (MQ=255) ggTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTTTAC‑TGGGGCCATTTATTGCCCGTTCTCTTTTTCCATCGTTTTGGCaa > 2:513067/1‑239 (MQ=255) tGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGTTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTGTTAC‑TGGGGGAATTTTTCTCCCGTCTTCTTTCCCCACACTTTCTGCAA‑tt > 2:715145/1‑238 (MQ=255) tGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TTGGGCAATTTTTTCCCAGGTTTCTATCACCATCGGTTCGGCAA‑Tg > 2:515593/1‑239 (MQ=255) tGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGAACTGGCGGGGATCGCGATGGGGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGTCGGTTCTAC‑TGTGGGAATTTATCGCCCGGTCTCTCTCACCCTCGCTTCGGCAA‑Tg > 2:511962/1‑239 (MQ=255) ttGCCATCGGGCTGCTCGTCGATGGTGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGgc < 2:642387/238‑1 (MQ=255) tGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGGGCTGTCAGCGGTATTTATTCCGATGGCATTTTTCGCCGGTTCTTC‑TGGGGCAACTTTTCTCCCGGTCTCTTTCACCCACGTTTCGGCAA‑GGgc > 2:349129/1‑237 (MQ=255) cGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGTTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TTGGGGAATTTATCGCCAGGTTTCTACCTCCCTCGTTTCGGCAA‑TGGGGCCttcgg > 2:214895/1‑237 (MQ=255) cGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TTGGGCAATTTATCGCCAGTTCTCTTTTACCATCGTTTTGGCAA‑TGGCCCTttctt > 2:760356/1‑238 (MQ=255) cGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGGGGGTATCGCGATGGGGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTTGGGGAATTTTTTCCCCAG‑TCTCTCTCACCACCGGTTCGGACA‑AGGCGCtt > 2:570780/1‑235 (MQ=255) ctCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTTTAC‑CGGGGGACATTTTCGCCCGTTTTTTTTCACCCCCGTTTTGGCaaaaggggcttttggtttggt > 1:185062/1‑220 (MQ=255) tggagatgggtaaaaggaccagggAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCa < 2:421165/217‑1 (MQ=255) cgATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAt < 2:746048/239‑1 (MQ=255) gttgaggCGATAGTGGGGGTGGAGAACGTCGAGCGAGGGATGAGGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAt < 2:79136/236‑1 (MQ=255) gattgagCGATAGGGGTGGGGGAGAACGTCGACCGAGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAt < 2:792468/232‑1 (MQ=255) agggtgCGATAGTGGTGGTGGAGAACGGCGAGCGCGTGATGAGGGGGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAt‑t < 2:93608/234‑1 (MQ=255) tgatgCGATAGTGGTGGTGGAAAACGTGGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑Tg < 2:603243/238‑1 (MQ=255) aTAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATTGCCCGGTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTTTTCTGGTGGCCT‑TGATTCTTa > 2:204801/1‑238 (MQ=255) tggtgggggggGAAAACGTCGAGCGCGTGAGGATGGAGGATAAGCCCCCGCCAAAAGAAGCGGCGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTTGTGGGTTTCGCGGTGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTAcc < 1:794450/237‑1 (MQ=255) ggtggtgggcGAGAACGTCGCGCGCGGTAGGATGGAGGATAATCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTAccc < 2:722174/238‑1 (MQ=255) tggAGAACGTCGGGCGCGGGATGATGGAGGATAAGCCCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑Gtt < 2:135021/238‑1 (MQ=255) gggAAAACGTCGAGCGCGTGAGGATGGAGGAAAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑Gtt < 2:129670/237‑1 (MQ=255) cGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGGATCGCGATGGAGCTGTCAGCGGTATTTATTCCGATGGAATTCTTCGGCGGTTCTAC‑TGGGGCAATTTTTCTCCCGTTTCTTATCCCCCTCCTTTCGGGAAGTGGCCGTTTCTTTTCT‑GTGGCAT‑TGTGTTTTAccccccgtgtttggggaa > 1:651967/1‑225 (MQ=255) cgggggcgTGATGATGGAGGATAAGCCCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACg < 2:310440/233‑1 (MQ=255) cggggcggtgtTGAGGGGGGCTAAGCCCCCGCCAAAAGAAGCGACGGAAAAAGCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCGGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACgc < 1:620799/232‑1 (MQ=255) cggagggggtgtgatgGAGGATAAGCCCCCGCCCAAAGAAGCGAAGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACg < 2:839638/227‑1 (MQ=255) cgcacgggTGAGGATGGGGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAAACGAGGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACg < 2:682627/231‑1 (MQ=255) cGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCCGTTTTCTATCACCATCGTTTTGGCAA‑TGGCGCTTTCTGTTCTGGTTGCAT‑TGATTCTTACCCCCGC‑Gtttttggcaaac > 2:478238/1‑228 (MQ=255) ggttggcgataaaactctCGCCAAAAGAGGCGCCGGAAAAATTGTTGTCGCAAATTCT‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTCCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAAcc < 1:349129/224‑1 (MQ=255) tAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTCC‑GTTATGTTCAACGCTGCTTAAACCCCtccttgcgt > 2:230836/1‑232 (MQ=255) accgcccGCCAAAAGAGCGGAAGGAAAAATTGCGGCCGCAAATTCA‑GGGCGCGCTGGTGGGTATCGCGATGGGTCTGGCAGCGGTATTTATTCCGATGGCATTCCTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCTTCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAAGCCGTCTCTGCTGAg < 1:290245/235‑1 (MQ=255) ccccccccAAAAGGGGCCGCGGAAAAATCGATGTCGCAAATGCA‑GGGCGCACTGGTGGGTTCCGCGATGGTGCTTGCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCa < 1:513067/237‑1 (MQ=255) cGCCAAAAGGAGCCACGGAAAAAGCGATGTCGAAAATTCA‑GGGCGCACTGGTGGGTATCGGGTTGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAc < 1:575515/239‑1 (MQ=255) aagaagCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCt < 2:223786/181‑1 (MQ=255) cgcGGAAAAATCGGTTTCGCAAATTCAGGGGCGC‑CTTGTGGGTATCGGGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCg < 1:459623/237‑1 (MQ=255) aCGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCg < 2:185062/238‑1 (MQ=255) cGATGGCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt < 2:366720/239‑1 (MQ=255) gATGTCGCAAATTCA‑GGGGCCACTGGTGGGTGTCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑GGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATATGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggtt < 1:541229/239‑1 (MQ=255) tctgggCGCCTTGGTGGGTTTCGGGATGGTGTGGTGCGGGGTATTTTTTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca < 1:515593/236‑1 (MQ=255) tccgggCGCACTGGTGGGTAGCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca < 2:450826/236‑1 (MQ=255) aCTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATTCACCGTTTTCTTTCACCCACGTTTCGGCCA‑TGGCGCTTTCTGTTCTTGTGGCATGTGTTTTTTCCCCCT‑C‑CTTCTGTTCCACGCCGCTTAAAACCCGCTTTTCTTGGACTCCCGGAAAAAAGGGGGGGGTCTTTGGGTGTTTTATTACCAACTTccgtc > 1:824548/1‑235 (MQ=255) tATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATAACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCCT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCCGCTCTTCTGAGCATCACGGAAATAAAGGCGGGTTTTTCGGTTGGTTTAATACCACCTTCCATCAAAACGTTa > 2:647125/1‑239 (MQ=255) cggtgtggtgcttgcatgtgtttttactCCCATGGCATGCTTGGGGGGTTCTGC‑GGGGGCCATTTACGTGCAGTTCTCTTTCACCTTCGGTTCGGAAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTTCCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAcc < 1:536674/217‑1 (MQ=255) cgcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTGTAC‑TGGGGCAATTTTTTGCCAGTTCTCTATCACCATCGTTTCGGCCA‑TGGCGCTTTTTTTTTTGGTGGAGT‑TGATTCTTTACCCCGC‑GTTGTTTTCCACACTGCTTATACCCGTCTCTTCTGAGCATCACGCAAATAAAGGCGGGTTTTTCGGTGTGTTTATTAACACCCTCgctataagcggtaaac > 1:378732/1‑224 (MQ=255) cgcgATGGTGCTGTCAGCGGAATTTATTCCGATGGCATTCTTCGGCGGTTTTAC‑TTGGGCCATTTTTCGCCCGTTCTCTCTCCCCATCCGTTCGGCAA‑TGGCCCTTTCTGTTCTGGTGGGCT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCCTTTCTGCTTAGCATCACGGAAAAAAGGGGGGGTTCTTCTGTTGGTTTTATA‑AACCTTCGACCaaaagggtaacac > 1:488214/1‑227 (MQ=255) cgtttgtgtTGTCAGTGTTTTTTTTTTCGTTGGCTTTCTTCGGCGGTTCTAC‑TGGGGCAATTTAACGCCAGTTCTCTAGCACCATCGTTGCGGCAA‑TGGCGTTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAc < 1:46433/234‑1 (MQ=255) cTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTTTCACCATCGTTTCGGCAA‑TGGGGCTTTCTGTTCTGTGGGCAT‑TTATTTTTTCCCCTGC‑GTTATTTGCAACGCTGCTTAAACCCGTCTCTGCTGGGCATAACGAAAATAAGGGGGGTTTTTTCGGTTGGTTTAATACAACCTCTGATCAAACGGTTAACcaccaccacaa > 1:226068/1‑234 (MQ=255) ttttCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaa < 1:627305/237‑1 (MQ=255) ttgcGATGGCATTCTTCGGGGGTTTGTC‑TGGGGCAATTTAGCGCCCGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTCTTTTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAGCCACTACACCAACAGCGTCGGCAAAATc < 2:442955/236‑1 (MQ=255) ttCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCCCCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATc < 2:287814/239‑1 (MQ=255) gtgtctttcttcGGCGGTTCTTC‑GGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTGCTGGTGGCAT‑TGATTCTTTCCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCgg < 1:487691/233‑1 (MQ=255) gtGGCATTTTTCGGCGGTTCCAC‑TGGGGCAATTTATCGGCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCgg < 2:719605/238‑1 (MQ=255) gtttttgttgtgTTTTAC‑TGGGGAAATTTATTCCCGGTTCCCCATCCCCTTCGTTCTGGCAA‑GGGCGCTTTCTGTTCTGGTGGCAA‑TTATTCTTACCCCTGC‑CGTATGGTCAACGCTGCTTAAACCCTGCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCca < 1:354344/228‑1 (MQ=255) aTTTTTCGGGGGTTTTAC‑TGGGGCAATTTATCGCCCGTTCCTTATCCCCCGCCTTTGGGCAA‑TGGCGCTTCCTGTTCTGGTGGCAT‑TGATTCTTCCCCCTGC‑GTTATGTGCAAGGGTGCTTAAACCCGCCTCAGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCACCAGCGTCGGCAAAATCCTCGGATCca < 1:97348/239‑1 (MQ=255) ttcGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAgg < 2:226068/239‑1 (MQ=255) ttcGGCGGGTCTTC‑TGGGGCAATTTTACGGCAGTTCTCTATCCCCATGGTTGCGGCAA‑TGGCGCTTTCCGTCCTGGTGGCAT‑TGATTGTTACCCCTGC‑GGTATGTGCAACGCGGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAgg < 1:511057/239‑1 (MQ=255) tcGGGGGTTTTCC‑TGGGGCAATTTATCGCCAGTTCTTTAGCCCCCTCGTTTCGGCAA‑TGGCGTTTTCTGTTCCGGTGGCAT‑TGATTCTTTCCCTTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGa < 1:230836/239‑1 (MQ=255) ggTTCTAC‑TGGGGCAATTTTGCGTCAGTTCTCTAGCGCCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCTT‑TGATTCGTACCACTGC‑GTTATGTGCAACGCTTCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATCCCACCTTCGATCATAGCGTTAACCACTACTCCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGat < 1:570780/238‑1 (MQ=255) tc‑TGGGGCAATTTAACGGCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACt < 2:106994/238‑1 (MQ=255) tc‑TGGGGAAATTTATCCCCTGTTTTTTTTCACCGTCGTTTCGGCAA‑GGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACt < 1:776904/238‑1 (MQ=255) aC‑TGGGGCAATTTACCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACt < 2:880800/239‑1 (MQ=255) gCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTa > 2:251762/1‑239 (MQ=255) tttctccccctttttctATCCCCATCGTTTGGGCTA‑TGGCGCTTTCTGTTCTGGGGGCAT‑TGAGTCTTCCCCCGGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATgcg < 1:251762/225‑1 (MQ=255) ttgcGCCATTTCTCTATCGCCCGCGTTTGGGCAA‑TGGCTCTTTCTTTTTTGGGGGCAT‑TGAGTCTTACCCCTGCTGTTA‑GTGCAACTCTGGTTCACCCCGTGTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCt < 1:476841/236‑1 (MQ=255) | TGTTCGGGATGGTGCTTGCCATCGGGCTGCTCGTCGATGATGCGATAGTGGTGGTGGAGAACGTCGAGCGCGTGATGATGGAGGATAAGCTCCCGCCAAAAGAAGCGACGGAAAAATCGATGTCGCAAATTCA‑GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTAC‑TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAA‑TGGCGCTTTCTGTTCTGGTGGCAT‑TGATTCTTACCCCTGC‑GTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGGACGATATTTACTGATCTATGCGCT > NC_000913/3416214‑3416667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |