Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,479,687 G→A 15.6% L307F (CTC→TTC)  kefB ← potassium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,479,6870GA15.6% 138.1 / 3.8 61L307F (CTC→TTC) kefBpotassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (32/19);  new base A (2/8);  total (34/27)
Fisher's exact test for biased strand distribution p-value = 1.71e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCG  >  NC_000913/3479482‑3479922
                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
cgttctacaaGCATCGGCTGGGTCATAATGTCAAGCGCTCCCGTCACCAGCACCACGCCCATTGGGTTCCCCTGAAATAAGTTTTTTGAAGACGCGGTAAAAAAGAGGACAAAGGCAACCTCACCACCCGGTCTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATTCAACAGCGCCACCAGTCACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTAt                                                                                                                                                                                                            <  1:558703/230‑1 (MQ=255)
       tcaGCAACGGGGTGGTCAACAGGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCGGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACCCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTAcc                                                                                                                                                                                                     <  2:768163/239‑1 (MQ=255)
       cgagcacggggttGGTCTTCTGGGAAAGCGTACCCTTCCCCAGCAACAACGCCATCTGGTCCCCCGGGAAAAAGCGTTGGGAAGAGGGGGAAAAAAAGGGAACAAAGGCAAACCCCCCCCCCTGCCTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTAcc                                                                                                                                                                                                     <  1:421476/227‑1 (MQ=255)
           cAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAAAATTTTCACCGCCACCAGCCCAACCACCCTTATCACTACCcaca                                                                                                                                                                                                 >  2:149954/1‑239 (MQ=255)
              cGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTTACCTCCGCACGCCATCCAATCCCGCCAGCAGAACCAGCCCCAAAATTTTCACCCCCCCCACCCCAACCACCCCTAacacccacccaaaaa                                                                                                                                                                                              >  1:581869/1‑228 (MQ=255)
                 cttGGTCATAATGGAAAGCGTCACCGTCACCAGCAACAACGCCATGTGGTCGCCCTGGAATAAGCGTTTGGAAAAAGCGGTAGAAAAGAGGACAAAGGCAAACTCCCCACCCTGCCTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTCCGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGa                                                                                                                                                                                            <  2:47845/236‑1 (MQ=255)
                     gTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATACCTACCCccaaccaatgct                                                                                                                                                                                        >  2:421476/1‑231 (MQ=255)
                      gctccacgaacagctgccccgtcacaACAAACAACGGCATCTGGGCGCCAGGAAAAAAGCTTGGATAAAAACCGATAGAAAAGAGGAAAACGGCAACCCGCCCACCCTGGCGCAACCGTCAACAAAACTGCACCCGCTCTTGGTTACGCACGCCTAACAATCGCGCTAGCACTTACAGCACGAGAATTATCGCCGCCACCAGCAAAACCCCGCTTATCACTACCCACAACAGATGGGt                                                                                                                                                                                       <  1:621020/220‑1 (MQ=255)
                           atgcaaggctccacctcccccAGCAACACCTCCTTGTGGTCGCCCGGGAAAAAGCTTGGAAAAGAGGCGGTGTAAAGAAGGACAAAGCAAAACCACCCCCCTGCACCAACACGCCCGCAAACCTCCATCCGCCTTAAGCCACGCACGCCATACAACCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAg                                                                                                                                                                                 <  1:304394/220‑1 (MQ=255)
                           atGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCAATACAATCGCGCCAGCAGATACAGCCCGAGACTTTTCACCGCCCCCACCACAACCACGCTTATCACCACCCccaaaaaaagggaaaaaa                                                                                                                                                                                  >  1:446437/1‑224 (MQ=255)
                                aaGCGTCACCGTCACCAGCAACAACGCCATCTGGTCCCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCCCCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCAcc                                                                                                                                                                             <  2:206042/238‑1 (MQ=255)
                                   cgtcaccgtcacCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCAATCCAATCGCGCAAGCAAACACAGCACGAGAATTTCAACCGCCACCAGACCCACCCAGCTTATCACCACCAACAAAAAAAGGGCCAAAAACAcccccc                                                                                                                                                                         >  1:222948/1‑237 (MQ=255)
                                    gtcacggtcaccACAAACAACGACTTCGGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGAAAAAGGCAAACTCCCCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGATCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGa                                                                                                                                                                         <  2:563624/238‑1 (MQ=255)
                                    ctcccgccccccacacaacaccccATCTGGTCCGCTGTGAACAAGCGTTGGAAAAAAGCGGTAAAAAAAGGGAAAAAGGCAACCCCCCCCCCCGGCCCCAACCCCCCAGCAAACTGCTCCCGTTCTGAGCTCCGCACGCCATCAAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGa                                                                                                                                                                         <  1:687335/216‑1 (MQ=255)
                                       accgtcaccAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGCGCTACTCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCCCAACCCCGCTTATCACCAACCCCAAAAGATGGGAATAAAGCACCCAGaagat                                                                                                                                                                     >  1:155767/1‑235 (MQ=255)
                                       accgtcaccAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAAATGGGTATAAAGCACCCCGCGGtt                                                                                                                                                                     >  2:889343/1‑239 (MQ=255)
                                                  caacaaCGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATg                                                                                                                                                           >  2:214638/1‑238 (MQ=255)
                                                   aacaacGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATGc                                                                                                                                                          >  2:518981/1‑238 (MQ=255)
                                                     caacGCCAGCTGGTCCCCCTGGAAAAAGCGTTGTAAAGAAGCGGTAAAAAAGAGGACAAAGGCAAACTCACCACCCTGACCCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGa                                                                                                                                                       <  2:515008/239‑1 (MQ=255)
                                                        ccgcagtgggTCGCCCTGAAAAAAGGTTTGTAAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCCCCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACag                                                                                                                                                    <  2:196396/231‑1 (MQ=255)
                                                            aTCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCCCAACCACGCTTATCACTACCCACAACATATGGTCATAAAGCACCCCGAGGTTGAGTGCCATGCCGACagaaga                                                                                                                                                >  2:850535/1‑236 (MQ=255)
                                                             tCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTGTAAAGCACCCCGAGGTTGAGTGACAGTCCGACAGAGATa                                                                                                                                               >  2:736052/1‑239 (MQ=255)
                                                              tgggccccacTGGAATAACGTTTTTGAAGAGGCGTTAGAAAAGAGGACAAAGGAAAACCCCCCCCCCTTACTACACAGCCCAGAAAACTCCATCCGCTCTGAGCTCAGCACCCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATaa                                                                                                                                              <  1:436610/230‑1 (MQ=255)
                                                                  tCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACCGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAAAAGGGACAAAAGCAACCCGCGGTTGAGTGACATCGCGCCAGGAAAAAAGaa                                                                                                                                          >  1:887849/1‑239 (MQ=255)
                                                                   cGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGAACGAGGTAAAGAAc                                                                                                                                         >  2:734816/1‑239 (MQ=255)
                                                                                     tgtgAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGATGGGTGACATGCCGACCGAAATAAAGAACAAACCGAGCAACAAg                                                                                                                          >  1:807220/1‑237 (MQ=255)
                                                                                         aaaaaggggaaaaaaaGAGGAAAAAGACAAACCCACCCCCCTGACTCAACCCGCCAGCAAACTGCACCCCCTCTGAGCTACCCAAGCCATACACTCGCGCCAGCAGATACAGCACGAGAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTg                                                                                                                    <  1:149954/227‑1 (MQ=255)
                                                                                          agaagCGGAAAAAAAGAGACAAAACGCAACCCCACCCCCCTTCACCAAACCCCCAGCAAACTCCATCCGCTCGGACCCTCCGACCCCAGAAAATCGCCCCATCAGATACAGCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACATAGATAAAGAACAAACCGAGCAGCAAGCCTTTg                                                                                                                    <  1:26104/238‑1 (MQ=255)
                                                                                            aagCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGAATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGCGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                 >  2:717181/1‑239 (MQ=255)
                                                                                             gggggaaGAAAAGAGGACAAAGGCAAACTCACCACCCTGCCCCAACACGCCAGCAAACTGCATCCGCACTGAGCTACGCACGCCATACAAGCGCGCAAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAg                                                                                                                 <  2:891294/232‑1 (MQ=255)
                                                                                                 gTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCCCCCCTATCACTACCCACAACAGATGGGTATAAAGCACCCCTGCGTTGAGTGACATGCCGACACGGATAAGAAACAACCCCAACAGCAAGACTTGAAAggggcg                                                                                                            >  1:764562/1‑236 (MQ=255)
                                                                                                 gTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAGAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATc                                                                                                            >  2:810445/1‑239 (MQ=255)
                                                                                                               aaaGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTAACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGAACGCGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGACCGATACCCGTTTccg                                                                                              >  2:316042/1‑238 (MQ=255)
                                                                                                                             caccCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGCCATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGAAAAACGATTCCAGATCATGGCGaaaa                                                                                >  1:304654/1‑236 (MQ=255)
                                                                                                                             caccCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGCCATGCCGACAGAGATAAAGAAAAAACCGAGCAGCAAGCCTTTGAAGAGATCGATAACCGTTTCCAGTTCATTGCGctat                                                                                >  2:11671/1‑239 (MQ=255)
                                                                                                                                  tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc                                                                                     <  2:228795/229‑1 (MQ=255)
                                                                                                                                  tGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGc                                                                                     >  1:228795/1‑229 (MQ=255)
                                                                                                                                   gACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCCACCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGACCAGCAAGCCTTTGAAGGGATCAATAGCAGTTTCCAATTCACGGCGATAGTCAAtt                                                                          >  1:564126/1‑239 (MQ=255)
                                                                                                                                   gACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAACGAACAAACCGAGCAGCAAGCCTTTGCAGGGATCGATAGCCGTTTc                                                                                                >  2:476303/1‑217 (MQ=255)
                                                                                                                                         acacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGcc                                                                    >  2:501297/1‑239 (MQ=255)
                                                                                                                                          cacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACa                                                                                                                                                     >  2:180681/1‑157 (MQ=255)
                                                                                                                                          cacGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGCTCGAAAACCGTTTCCAGTTCATGGCGAAATTCCCTTTCCCCca                                                                   >  1:588571/1‑239 (MQ=255)
                                                                                                                                                  cAAACTGCATCCGCTCTGAGCTACGCACGCCATAAAATCGCGCCAGCAGATACAGCACGAGAATTTTCACTGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCAGTTTCACATTCATGGCGATCTTCCCTTTCCGCCAGCAGCAc                                                            >  2:439926/1‑238 (MQ=255)
                                                                                                                                                     aaCTGCACCCGCCCAGAGCTCAGCCCGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTCCACCGCCAACAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGcc                                                         <  2:417663/239‑1 (MQ=255)
                                                                                                                                                         tGCATCCGCTCTGAGCAACGCACGCCACACAAGCGCGCCCGCAGATACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc                                                      <  2:764562/239‑1 (MQ=255)
                                                                                                                                                         tGCACCCCCTCTGAGCTACGCCCGCCATACAATCGCGCCAGGAGAAACAGCCCGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGc                                                      <  2:588571/239‑1 (MQ=255)
                                                                                                                                                                        ccacgcccgcCTTACAATCGCGCCAGCATATACAGCCCGAAAATTTCCACCGCCACCAGCACAACCACGCTTAACACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAg                                       <  2:807220/237‑1 (MQ=255)
                                                                                                                                                                        caccgcccgccAAAAAAACGCGCAAGCAGATAAACCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGa                                        <  2:328297/235‑1 (MQ=255)
                                                                                                                                                                                          cgcgCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGGTTCCAGTTCATGGCGATCTTCACTTTCCGCCAGCAGCACGCCCGCCATAAATGCACCAGACGCCAACAACAGCCCCac                     >  1:42780/1‑238 (MQ=255)
                                                                                                                                                                                            cgcCAGCAGATACAGCACGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCCCTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  2:581869/239‑1 (MQ=255)
                                                                                                                                                                                            cgcCAGAAGATACAGCAGGAAAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  2:887849/239‑1 (MQ=255)
                                                                                                                                                                                            cccgacgataaaacgCAAGAAAATTTTCACCGCCACCAGCGCAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  2:436761/225‑1 (MQ=255)
                                                                                                                                                                                            cccccgcagTTACACCACGAAAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgcg                   <  1:892888/234‑1 (MQ=255)
                                                                                                                                                                                              ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCTCCAGCAGCACCCCCCCCAAAAAAGTACCAAGCGCAAACACCAGCCCCAacga                  >  1:314156/1‑237 (MQ=255)
                                                                                                                                                                                              ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAgccc                  >  2:892888/1‑236 (MQ=255)
                                                                                                                                                                                              ccAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAACACGCCCGCAATCAAGGAACCGAACGCCCTCGCCACccccccccc                  >  1:557975/1‑233 (MQ=255)
                                                                                                                                                                                                      atcactccGAGAATTTCCCCCGCCACCAGCCCAACCCCGCTTATCTCTACCCACAACAGATGGGTATAAAGCACCCCGTGGTTGAGTGACATGCCGAAAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATaaa         <  2:564126/232‑1 (MQ=255)
                                                                                                                                                                                                           cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCACGCCTTTGAAGGGATCTATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCCTCGCCAGCCCCAGCGCATCCATAAACAAt     >  2:193221/1‑238 (MQ=255)
                                                                                                                                                                                                           cACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGAGCCATCGACAGCCCCCGCCAATCCATaaaacaa     >  1:130536/1‑234 (MQ=255)
                                                                                                                                                                                                             cGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg  >  2:772197/1‑239 (MQ=255)
                                                                                                                                                                                                             cGAAAATTTTCCCCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCg  <  2:557975/239‑1 (MQ=255)
                                                                                                                                                                                                                |                                                                                                                                                                                                                                           
AGCTTCATCAGCAACGGCGTGGTCATCATGGAAAGCGTCACCGTCACCAGCAACAACGCCATCTGGTCGCCCTGGAATAAGCGTTGTGAAGAAGCGGTAGAAAAGAGGACAAAGGCAAACTCACCACCCTGACTCAACACGCCAGCAAACTGCATCCGCTCTGAGCTACGCACGCCATACAATCGCGCCAGCAGATACAGCACGAGAATTTTCACCGCCACCAGCACAACCACGCTTATCACTACCCACAACAGATGGGTATAAAGCACCCCGAGGTTGAGTGACATGCCGACAGAGATAAAGAACAAACCGAGCAGCAAGCCTTTGAAGGGATCGATAGCCGTTTCCAGTTCATGGCGATATTCACTTTCCGCCAGCAGCACGCCCGCAATAAATGTACCGAGCGCCATCGACAGCCCCAGCGCATCCATAAACAATGCG  >  NC_000913/3479482‑3479922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: