Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	NC_000913	4,001,644	Δ5 bp	100%	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001644	4001648	5	85 [0]	[0] 85	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001643	0 (0.000)	79 (1.480)	58/414	NT	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001649 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAA  >  NC_000913/4001422‑4001871                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                tcccggtcatgctgagcccattgcaacgggaaaataaccgactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgacccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatc                                                                                                                                                                                                                                 <  2:494867‑M2/1‑1 (MQ=255) tcccggtaatggtgagcgcatttcacctggaaaataaccgactggcccggcgggaagtcgaagagtcacaacccctggtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatc                                                                                                                                                                                                                                 <  2:624859‑M2/1‑1 (MQ=255)    cggtcatgctgagcgcatttcaactggaaaataaccgacttgaccggcttgaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatct                                                                                                                                                                                                                                <  2:493022‑M2/2‑1 (MQ=255)          tgctgactgcatttcagatggaaaattaccggctgaccggggtggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttctt                                                                                                                                                                                                                         <  2:291773‑M2/9‑1 (MQ=255)           gctgagcgcatttcaactggaaaataaccgactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcataggcacggttctttgaagacgaggacggcctgcatAtccttctttt                                                                                                                                                                                                                       >  2:617358‑M2/218‑226 (MQ=255)                    atttcaactggaaaataaccgtctgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAg                                                                                                                                                                                                              <  2:592810‑M2/20‑1 (MQ=255)                      ttcaactggaaaataaccgactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgccgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagactcggcacgtttctttgaagacgacgacggcctccatCTCCTTCTTCTTCTTTGAAGa                                                                                                                                                                                                             >  2:278537‑M2/207‑227 (MQ=255)                         aatgggaacataaccgattgacccggcgggaaatcgaagggtcacaaccccttggaaatgccggatggattgatcttgtcgaaccggacggcgacgggcggctgcgcgtacaatctgaacttggccagagccttgcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCg                                                                                                                                                                                                         <  1:432940‑M2/25‑1 (MQ=255)                         aactggaaaatacccggctgacccggctggaagtcgaagagtcacaaccccttgtaaaggcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCg                                                                                                                                                                                                         <  2:605571‑M2/25‑1 (MQ=255)                              gaaaataaccgactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaccccgcccggacatggacgacatcgaagcttcggaacgtttctttgaagacgccgacggcctgcaacccctccttcttcttttgagagtgggaaga                                                                                                                                                                                                    >  1:625744‑M2/199‑214 (MQ=255)                                aaaaacccgggtgacccgggcggaattcgaaggtgcactaccccttgtaaatgcgttatggattgctcttgtcgaaccggaacacgacggcggcctgcgcgtacaatctgaacttggccagagccttgcaccccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATc                                                                                                                                                                                                  <  1:359348‑M2/32‑1 (MQ=255)                                   taacccgtcgccccggggggacgtcgaagagtcacaaccccttggaaatgcagtatggattgatcttgccgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACg                                                                                                                                                                                               <  1:618233‑M2/35‑1 (MQ=255)                                        gactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagctcggcaacccgcccggaactggaagacatcgaaggatcggcacgtttctttgaagccgacgaaggccggcacCTCCTTCTTCTTCTTTGaagatgcggaaggtacagccggt                                                                                                                                                                                          >  2:324897‑M2/189‑217 (MQ=255)                                        gactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggacgacatcgaagcatcggcacgtttctttgaagccaacgacggccggcctCTCCTTCTTCTTCTTTGAAGTTGCGGAAGAGCACCCcgga                                                                                                                                                                                          >  1:213421‑M2/189‑227 (MQ=255)                                        gactgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGt                                                                                                                                                                                          <  2:421558‑M2/40‑1 (MQ=255)                                         actgacccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggaccgtttctttgaagacgacgacggcctgcatCTCCTTCTCCTTCTTTGGATGTGCGGAAGGTCAATccggga                                                                                                                                                                                         >  2:600007‑M2/188‑226 (MQ=255)                                             acccggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcccgtttttttgacgacgacgacgccctgcatCTCCTTCTCTTTCTTTGAAGATGCGGAAAATACCCCCGGTAACTc                                                                                                                                                                                     >  1:447265‑M2/184‑228 (MQ=255)                                                 ggctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgggcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACt                                                                                                                                                                                 <  2:88909‑M2/49‑1 (MQ=255)                                                  gctggaagtcgaagagtcacaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTTTTCTTTGAAGATGCGGAAGATCACGCCGGTAAACCcaccg                                                                                                                                                                                >  1:542253‑M2/179‑226 (MQ=255)                                                           cgaagagtccacaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTa                                                                                                                                                                       <  2:338309‑M2/59‑1 (MQ=255)                                                              agggtccaaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgccgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTAcc                                                                                                                                                                     <  2:431310‑M2/61‑1 (MQ=255)                                                               ggttacccaccccttggagattgattagggattgatctggtcgaaccgggcgacggcgagcacctgcgggtacaatctgaaattggccaggacctggcaacccgcccggaactggaagacatcgaatcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAGGATCACGCCGGTAACTCCACTGTGGCATTTACCa                                                                                                                                                                    <  1:257321‑M2/62‑1 (MQ=255)                                                                    acaaccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATc                                                                                                                                                                  >  2:400398‑M2/161‑224 (MQ=255)                                                                       accccttggatattgagtatggattgatcttggtgaaccggacgacgacgggcgcctgcgtgtacaatcggaagttggccagagccgggcaacccgcccggaactggaagacaacgaagcatcggcaagtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                                                                                                           <  1:278537‑M2/71‑1 (MQ=255)                                                                        ccccttgtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATg                                                                                                                                                           <  2:542253‑M2/71‑1 (MQ=255)                                                                            ttgtaaatgcagtctggattgaccttggcgaaccggacgacgacgagcgcctgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcg                                                                                                                                                       <  2:343665‑M2/75‑1 (MQ=255)                                                                              gtaaatgcagtatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcgtc                                                                                                                                                     <  2:204809‑M2/77‑1 (MQ=255)                                                                                     caggagggattgaccttgccgaaccggacgacgacgagggactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTAct                                                                                                                                             <  2:436636‑M2/85‑1 (MQ=255)                                                                                        tatggattgatcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATAACGCCGGTAACTCCCCTGTGGCATTTACCATCCGGGATGTTCGTCTGTTTActct                                                                                                                                           >  2:518067‑M2/141‑227 (MQ=255)                                                                                           ggattgatcttggcgacccggacgacgacgagcgactgcgcgtacaatcggaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcg                                                                                                                                        <  2:328915‑M2/90‑1 (MQ=255)                                                                                                  tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcctCTCCTTCTTCTTCTTTGAAGATGCGGAAAATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGGATGGCCGCTGGTTTCCTctccctgaggg                                                                                                                                  >  1:52743‑M2/131‑224 (MQ=255)                                                                                                  tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAGGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTactctccgtgacgctg                                                                                                                                >  2:654960‑M2/131‑223 (MQ=255)                                                                                                  tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGCTCACGCCGGTAACTCCACTGTGGCCTTTCCCATCCGTGATGGTCGTCTGTTTTctctgcgtgagaggg                                                                                                                                >  1:366493‑M2/131‑224 (MQ=255)                                                                                                  tcttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcgtgagcgtg                                                                                                                                >  2:129653‑M2/131‑228 (MQ=255)                                                                                                  tcttgtcgaacaggacgacgacgagcgactgcgcgtaaaatctgaacttggccagagcctggcaacccgcccgtaactggaagacatcgcagcatcggcacgtttctttgaagacgaggacggcctgcatCTCCTTCTTCTTCTTTGAAGAAGCGGGAGATCACGCCGGTAACTCCCCTGTGGCATTTACCATACGTGCTGGGCGTCTGTTTACGCTgcgtgagcgtg                                                                                                                                >  1:76342‑M2/131‑228 (MQ=255)                                                                                                      gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGTTGCGGAAGTTCCCGCCGGTAACTCCCCTGTGGCATTTACCCTCCCTGCTGGCCGCCTGTTTCTCCTCCGTGGGGGTGAacc                                                                                                                            >  1:587687‑M2/127‑227 (MQ=255)                                                                                                      gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcg                                                                                                                                        <  2:202158‑M2/90‑1 (MQ=255)                                                                                                      gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTgcg                                                                                                                                        >  1:202158‑M2/127‑216 (MQ=255)                                                                                                      gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGGAACTCCACTGAGGCATTTACCATCCGTGATGGTGGTCTGTTTACTCTCGCTGAGCGGGAACt                                                                                                                            >  2:428643‑M2/127‑228 (MQ=255)                                                                                                        ccaaccggacgacgacgagccactgcgcttacaatctgaacttggccagggcctggcaccccgcccggacctggaagacatcgaagcatcggcccgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTg                                                                                                                           <  2:402961‑M2/103‑1 (MQ=255)                                                                                                         gaaccggaccgcgacgggcggctgcgtgtacaatcggaacttgcgcagagcctggcaccccgcccgagactggaagacatcgaatcatcggcaagtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGcc                                                                                                                         <  1:427723‑M2/105‑1 (MQ=255)                                                                                                           accggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTCCTCTGCGTGAGCGTGAACTg                                                                                                                           >  2:159329‑M2/122‑224 (MQ=255)                                                                                                            ccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTTTGGCATTTACCATCCGGGCTGGTCGTCTGTTTATTCTCCGTGAGCGGGAACtgccccc                                                                                                                      >  1:151770‑M2/121‑226 (MQ=255)                                                                                                              ggacaacgacgagcgacggggcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTTTTTACTCTGCGTGAGCGTGAACTGCCCGCtt                                                                                                                    <  2:587687‑M2/110‑1 (MQ=255)                                                                                                               gacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGGTAGTGTCGTCTGTTTACTCTGCGTGCGCGTGAACTGGCCGCttt                                                                                                                   >  1:467428‑M2/118‑228 (MQ=255)                                                                                                                acgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGGCGTTTTACTCTGCGTGGCCGGGAACTGCCCCCtttt                                                                                                                  >  1:620545‑M2/117‑228 (MQ=255)                                                                                                                    cgccggccgcttgcgcgtaacatctgaatttggccacggcctggcaacccgcccggacctggaagacatcgaagcatcggcacgtttctttgaagccgacgacggcctgcttCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc                                                                                                              <  1:324897‑M2/116‑1 (MQ=255)                                                                                                                                           ctgaacttggccgggcccttgccccccgcccggaactggaagacaacgaagcaccggcacttttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTGTAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGc                                                                                      <  1:518067‑M2/139‑1 (MQ=255)                                                                                                                                           ctgaacttggccagggcctggccacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGc                                                                                      <  2:54699‑M2/139‑1 (MQ=255)                                                                                                                                           ctgaacttggccagagcctggcaacccgcccggaactgaaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGc                                                                                      <  2:568115‑M2/139‑1 (MQ=255)                                                                                                                                              aacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcgcgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGCAGATCACGCCGGCAACTCCACTGTGGCCTTTCCCATCCGTGATGGTCGTCTGTTTACCCTGCGTGAGCGTGACCTGCCCGCTTTTCTTCT‑TTCTGGTATGGGTGCCCGGAGCCAg                                                                                   >  1:444663‑M2/87‑228 (MQ=255)                                                                                                                                               aggtggccggaccctgggagcccggccggaacgggaagaactccaaccttcggcacctttctttgaaggcgacgccggcccgcatCTCCTTTTTTGTCTTTGAAGATGGGGAAGATCACGCCGGTAACTCCTCTGTGGCATTTACCATCCGTGATGGTCGTTTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGt                                                                                  <  1:428643‑M2/143‑1 (MQ=255)                                                                                                                                               acttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTCCCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTACCGTATGCGTGCCCGTAGccaat                                                                                  >  1:566747‑M2/86‑226 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTcc                                                                                                                                                                                    >  1:211764‑M2/81‑126 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCCTCCGTGATGGTCGTCTGTTTACTCTGCGGGAGCGTGAACGGCCCGCTTTTCGTCG‑GTATCGTATGCGTGCCCGTAGCCCTTCGAt                                                                              >  1:152353‑M2/81‑227 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTCCTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGAt                                                                              >  2:476468‑M2/81‑227 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGGCT‑GTATCGTATGCGTGCCCGTAGCCAGTCgag                                                                              >  2:166761‑M2/81‑226 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGCCT‑GTATTGAATCCGTGCCCGTGACCAGTCGAt                                                                              >  2:576818‑M2/81‑227 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGCCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGAt                                                                              >  2:281732‑M2/81‑227 (MQ=255)                                                                                                                                                    gccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGCGCTGGTCGTCTGTTTACTCTGCTTGGGCGTGAACTTCCCGC‑TTTCGCCTGgtatcgtatacgggccctaaaccagccacag                                                                             >  1:326595‑M2/81‑214 (MQ=255)                                                                                                                                                     ccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCa                                                                                                                                                                                   <  2:211764‑M2/47‑1 (MQ=255)                                                                                                                                                         agcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTATTCTGCGTGAGCGGGAACTGCCCGCTTTTCGGCT‑GTATCGTATGCGTgcccgtgcccagacactgggcac                                                                        >  1:532898‑M2/76‑217 (MQ=255)                                                                                                                                                              ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTa                                                                                                                                                                                         >  2:526882‑M2/71‑111 (MQ=255)                                                                                                                                                              ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCATGCCCGTAGCCAGTCGATGGTAGACGGTa                                                                   >  2:301990‑M2/71‑228 (MQ=255)                                                                                                                                                              ggcaacccgcccggaactggaagacaccgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTa                                                                   <  2:467428‑M2/158‑1 (MQ=255)                                                                                                                                                               gccccccccccggaactggaaagacttgaagcctcgcccggtttctttgaagacggcgccggccggcatCTCCTTTGTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGGGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTaa                                                                  <  1:600007‑M2/159‑1 (MQ=255)                                                                                                                                                               gcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                                                                                                                        <  1:526882‑M2/42‑1 (MQ=255)                                                                                                                                                                   cccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGc                                                               >  2:34216‑M2/66‑227 (MQ=255)                                                                                                                                                                          gggactggaagacatcgaagcatcggcacgtttttttgaaggcgtcgccggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCCCTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGt                                                       <  1:617358‑M2/170‑1 (MQ=255)                                                                                                                                                                            aactggaagccatccaagcatcggctggtttctttgaagacgccgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGtt                                                      <  2:45627‑M2/171‑1 (MQ=255)                                                                                                                                                                            aactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCCCGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGtt                                                      <  2:447265‑M2/171‑1 (MQ=255)                                                                                                                                                                             actggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGGATGCGGTCCCGTAGCCAGGCGAGGGTAGAGGGTAACCCCTACGAGttgt                                                    >  1:457076‑M2/56‑227 (MQ=255)                                                                                                                                                                                ggaagcctccgaagcatcggcacgtttctttgaaggcgacgacggcctgcatCTCCTGCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTtgctgc                                                 <  1:181964‑M2/176‑1 (MQ=255)                                                                                                                                                                                       atcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACCGTGGCCTTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCTTATGCGTGCCCGTAGCCAGTCGCGGGTAGCCGGTAACGCCTACGCGTTGCTGCTGGGTCt                                          >  1:342435‑M2/46‑228 (MQ=255)                                                                                                                                                                                                  ggcgcttttctttgaagacgacgacggcctgaatCTCCCTCTTCTTCTTGGAAGATGCGGAAGAGCACGCGGGTAACCCCACGGGGGCATTTACCATCCGTGATGGTCGTCTGTTTTCTCTGCGTGAGCGTGAACTGCCCGCTTGTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCa                               <  1:476468‑M2/194‑1 (MQ=255)                                                                                                                                                                                                         ttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTATACGGTAACGCCTCACAGTTGCTGCTGGATCTGTTCGAaaccaaaaagga                        >  1:64813‑M2/28‑224 (MQ=255)                                                                                                                                                                                                            tttgaaagcgacgccggcctgcatCTCCTTTTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACa                     <  2:213421‑M2/204‑1 (MQ=255)                                                                                                                                                                                                                  gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGc               <  2:151770‑M2/210‑1 (MQ=255)                                                                                                                                                                                                                          cggcctgcatTCCCTTCTTCTTCTTTGAAGGTGCGGAAGATCGCGCCGGTAACTCCACGGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGaaa       <  1:576818‑M2/218‑1 (MQ=255)                                                                                                                                                                                                                            gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAAt      <  2:620545‑M2/219‑1 (MQ=255)                                                                                                                                                                                                                             cctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtc                                                                                                                                                        >  1:596379‑M2/8‑81 (MQ=255)                                                                                                                                                                                                                              ctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgtcg                                                                                                                                                       <  2:596379‑M2/75‑1 (MQ=255)                                                                                                                                                                                                                               ttcatCTCCTTCTTCGTCCTTGAAGCTGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGaa  <  2:271357‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                               tgcatTTCCTTCTGTTTCTTTGAAGATGCGGAAGGTCCCGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGaa  <  2:221369‑M2/223‑1 (MQ=255)                                                                                                                                                                                                                                 catCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCTTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGCGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAAAAGTTGGAAGGTAAAATTGa   >  1:291038‑M2/4‑225 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                GTCATGCTGAGCGCATTTCAACTGGAAAATAACCGACTGACCCGGCTGGAAGTCGAAGAGTCACAACCCCTTGTAAATGCAGTATGGATTGATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCT‑GTATCGTATGCGTGCCCGTAGCCAGTCGATGGTAGACGGTAACGCCTACGAGTTGCTGCTGGATCTGTTCGAAACCAAAATCGAACAGTTGGCAGATGAAATTGAA  >  NC_000913/4001422‑4001871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑