Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT100.0% 122.2 / ‑6.7 38R395C (CGT→TGT) icdisocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (18/19);  minor base G (1/0);  total (19/19)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTAG  >  NC_000913/1196084‑1196529
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
gtatcgCCCCGTGGGCAAACATGCGTTGCGAATGGGTCCGGGTTGAAAGCACCCACGGTACGTGCG‑CGAAATATGCCGGTCAGGACAAAGTAAATCCTGG‑‑‑‑‑‑‑‑‑‑‑‑CTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTATCCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                                                                                                                <  1:430846/228‑1 (MQ=16)
          tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCGACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGGTTGGAAGGGGCATACAAACGGAAAAACCAAACCTAAGACTCTGGGTGTTGGATGGATGgcgc                                                                                                                                                                                                                  >  1:463425/1‑228 (MQ=255)
          tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgcgc                                                                                                                                                                                                                  >  2:391243/1‑228 (MQ=255)
           ggTGCAAACATCGGAGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGCTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGGTGGTCTCATGGATGgcgca                                                                                                                                                                                                                 >  1:107108/1‑227 (MQ=255)
             tggaaaCATCGGTGAAGAATGCGGCCTGTTTGAAGCCACCCACGGTAC‑GGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                                                                                                                <  2:140438/224‑1 (MQ=255)
                      cGGTGACGAATGGGCCCTGTTTGAAGCCACCCACGGTAA‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                                                                                                                                                                                      <  1:57708/228‑1 (MQ=255)
                      cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                                                                                                                                                                                      >  1:638526/1‑228 (MQ=255)
                                            gAAGCCACCCACTGATC‑TGGGCCGAAATATGGCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGACACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                                                                                                                <  1:406519/228‑1 (MQ=255)
                                                    ccacGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa                                                                                                                                                                        <  2:638526/228‑1 (MQ=255)
                                                      cctgTAC‑TGCGCCGAAATATCCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa                                                                                                                                                                      <  2:323888/225‑1 (MQ=255)
                                                              tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGGGACGCGATCATCGAAAACATGTa                                                                                                                                                               >  2:92621/1‑228 (MQ=255)
                                                                   cGAAATATTGTGGTCAGGACAAAGTAAATCCTGGTTCTATTATTCTCTGCGGTGAGATGATGCTGCGACACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc                                                                                                                                                          <  1:245210/228‑1 (MQ=21)
                                                                    gAAATATGCCTGTCAGGACAAAGTAAATCATGGCTCTAGTATGCCCTGCGCTGATATGATGCTGAGCCACAGGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCg                                                                                                                                                         <  2:521614/228‑1 (MQ=21)
                                                                          tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGATTTTGGGGACGCGATCATACAAAACAATTAATGCCGTAGttt                                                                                                                                                   >  1:359035/1‑228 (MQ=255)
                                                                           gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg                                                                                                                                                  <  1:391243/228‑1 (MQ=255)
                                                                                 cAGGACAAAGTAAATCCTGGCTCTATTATTCTGTCCGGTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt                                                                                                                                            <  2:88796/228‑1 (MQ=255)
                                                                                   ggACAAAGTAAATCCTGGCTCTAGTATTCTCGCCGCGGAGAGGATGATGAGCCACATGGGTTGGACCGAAGCGGGTGAATTAATTGTTAAAGGTATGGAAGGCGCAATAAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAttt                                                                                                                                          <  2:245621/228‑1 (MQ=21)
                                                                                    gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                                                                                                         <  2:128013/228‑1 (MQ=255)
                                                                                         tgTAAATCCGGTCTTTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAc                                                                                                                                    <  2:348378/227‑1 (MQ=255)
                                                                                                   ggCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTaa                                                                                                                          <  2:463425/228‑1 (MQ=255)
                                                                                                                  tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAAGGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTGTTTAAATTTATTAACGGGAGCGTAACGCTCCCgttg                                                                                                               >  2:386269/1‑224 (MQ=255)
                                                                                                                    cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGTTCCCGTTGtttttt                                                                                                         >  2:587451/1‑228 (MQ=255)
                                                                                                                      cTGAGATGATGATGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCAGCGAAAAAATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGTGTacgccgacaggtggttttttg                                                                                                       >  1:79997/1‑208 (MQ=21)
                                                                                                                          gattgtgctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg                                                                                                   <  2:107108/223‑1 (MQ=255)
                                                                                                                              atgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATGGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                                                                                                        >  2:563571/1‑203 (MQ=255)
                                                                                                                              atgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATCGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACg                                                                                                                        <  1:563571/203‑1 (MQ=255)
                                                                                                                                         caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTAAGAGTTTGGGGACGCGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATc                                                                                    >  2:348721/1‑228 (MQ=255)
                                                                                                                                              gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGCGCGTAACGCTCCCGTTGTTTTTTGTTAGGTTTCTAAAGGTTATAAAAAt                                                                               >  1:207009/1‑228 (MQ=255)
                                                                                                                                                 ggACCGAAGCGGCTGACTTACTTGTTAAAGGTATGGAAGGCGCAATCAACGAGACAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGCTTGGTGACGCGATCATCGCAAACATGTAATGCCGTAGTTTGTTAACTTTATTAACGGGAGCGTAACGCTCCCGTGTTTTTTTGTTTGGCTGTTAACGGTTATCAAAActtt                                                                            >  1:125643/1‑228 (MQ=255)
                                                                                                                                                                          tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAAAGTTAAGAGTTTGGTGACGCGATCATCGAAAAAATGTAATGCGGTAGTTTGTTAAATTTATTAACGGTAGCGTATCGCTCCCGGTGTTTTTTGTTAGGCTGCAAACGGTTATCAAAATTTTATCAACAAAAGTTATCAAAACCCCt                                                   >  2:52338/1‑228 (MQ=255)
                                                                                                                                                                                  tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAg                                             >  2:338887/1‑226 (MQ=255)
                                                                                                                                                                                         cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgg                                                                                                                                                                                                                         >  1:109121/1‑47 (MQ=255)
                                                                                                                                                                                          gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                                                                                                        <  2:109121/47‑1 (MQ=255)
                                                                                                                                                                                                                 cTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGtt            <  2:79997/228‑1 (MQ=255)
                                                                                                                                                                                                                  tttGACTTCGGGTGTCTTATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGGGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTc           <  1:92621/226‑1 (MQ=255)
                                                                                                                                                                                                                          cGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTctactacta   >  2:493863/1‑228 (MQ=255)
                                                                                                                                                                                                                           gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAGCATGTACTGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTATGCTGGCCGGTCAGGCGGTATGTATACTACTAg  >  2:486008/1‑228 (MQ=39)
                                                                                                                                                                                                                           gAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGGTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTCGTTTTAGGGTAGGCTGGCCGTTCAGGGGGAAGTTctaccaaaaa  >  1:57737/1‑222 (MQ=38)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
GTATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTAC‑TGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTAG  >  NC_000913/1196084‑1196529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑