Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,145,056 T→G 20.0% G597G (GGT→GGG yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,0560TG20.0% 95.4 / 5.3 51G597G (GGT→GGGyegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (12/27);  new base G (10/2);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 2.11e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGAATAAAGAAGGCGAAGTCGAACGTCTCCTCGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCA  >  NC_000913/2144835‑2145282
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
aggAATAAAGAAGGCGAAGTCGAACCGTTCCTCGGCATAAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCg                                                                                                                                                                                                                                <  1:72928/226‑1 (MQ=255)
            ggCGAAGTCGAACGTCTCCTCGGCATCAATATGGATATGGCCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAAGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGgtg                                                                                                                                                                                                                   <  2:173339/228‑1 (MQ=255)
             gCGAAGTCGAACGTCTCCTCGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGTGAAGATGGGCGGCTGGAAGCAGGAAGAAACGTTAGTGGTTcc                                                                                                                                                                                                                                 >  2:72928/1‑214 (MQ=255)
             gCGAAGTCGAACGTCTCCTCGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAAAGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGTTATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAAATGAGCGGCTGGACGCAGGAAGAGACGTTTGGTGTTCCGCTCCTGCCGgtgt                                                                                                                                                                                                                  >  2:133217/1‑228 (MQ=255)
               gAAGTCGAACCTCTCCTCGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTg                                                                                                                                                                                                               <  2:542050/228‑1 (MQ=255)
                           ctcctcGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTTCGCTCCTTACGGTGTTGCATATTACttt                                                                                                                                                                                                    >  2:506086/1‑227 (MQ=255)
                                 ggCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGCGAAGATGAGCGGCTGGCCGCAGGAAGACGCGTTAGGGGGTCCGTTCCTGACGGGGTTGCATATTACTTTTgtggca                                                                                                                                                                                             >  2:71643/1‑223 (MQ=255)
                                   caataaTATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTTTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACaa                                                                                                                                                                                           <  1:444887/224‑1 (MQ=255)
                                   cATTAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACaa                                                                                                                                                                                           <  2:247304/228‑1 (MQ=255)
                                     tCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGTGAAAACGGGCGGCGTGACGCCGGAAAAAAACGTGGGGGTTCCGGCCCTCACGGGGTTGCAAAATAATTTTGGCGCAAACg                                                                                                                                                                                         >  1:268028/1‑228 (MQ=255)
                                      cAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACgg                                                                                                                                                                                        >  2:611484/1‑228 (MQ=255)
                                      aaaTATGGATATGGCCGAAGTGAAACAGCTTAAAGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTTCGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACgg                                                                                                                                                                                        <  1:621125/227‑1 (MQ=255)
                                             gATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGGAGCAGGAAAAAGCGTTAGGTGGTCTGCTCCTGACGGGGTTGCAAATTAATTTTTGCGGCAAAgggaaacta                                                                                                                                                                                 >  1:247304/1‑221 (MQ=255)
                                                 tGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTTCCCTCCTGGCGGGTGTTCAATGTACTTTTTGCGACCACGGAACATTAATgg                                                                                                                                                                             >  2:282066/1‑228 (MQ=255)
                                                 tGACCCGAGTGAAACCGGTTAACGGGGCATTGTTTCAGGAAAAAGAGCGTCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATgg                                                                                                                                                                             <  1:127592/228‑1 (MQ=255)
                                                   aCCGAAGTGGAACAGGTTAAGGTGGGTTTGGTTCAGGAAAAAAGGCGCCTGGACATTACGCTTGCTTCCATCGGTGAAGCCGTGGTCTGGAGTGAGATGGCGATGAAAATTACCTGTATGAATCCAGTGGGGTAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGTCGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGgag                                                                                                                                                                           <  1:225211/228‑1 (MQ=255)
                                                   aCCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGGGTTCCGCCCCTGACGGGGTTGCAAATTACTTTTGGCGACAACGGACCCTTAATgggg                                                                                                                                                                           >  2:438579/1‑226 (MQ=255)
                                                       aaGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGGCTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGaaaa                                                                                                                                                                       >  2:65693/1‑226 (MQ=255)
                                                              aCAGTTTAACGAGGCAGTGTTTTAGGAAAAAGGTCGCCTTCAAATTACGGTTGATTCCATCGGTGAAGCCGTGGTCTGTAGTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTAc                                                                                                                                                                  <  2:606750/226‑1 (MQ=255)
                                                                  cTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAAATGAGAGGGTGGACGCAGGGAGAAGAGTTAGTGGTTCCGCTCCCGGCGGGGTTGCCTCATACTTTTTGCGACACAGGACCCTTAAAGGAGAACATTTTCaaagcc                                                                                                                                                            >  1:326143/1‑223 (MQ=255)
                                                                            cATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAATAAGCGTTAGGGGTTCCGCTCCTGACGGTGTTGCATATTACTTTTTGCGACAACGGACCATTAATGGAGAAAATTTAAAGTGCCGACACCTCAc                                                                                                                                                  >  2:481668/1‑228 (MQ=255)
                                                                             aTTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGGGGCCGGACCGCGGAAGAATCGTTTGGGGTTCCGCTCCCGCGGGGGTTCGCAATTTCTTTTTGGGCCAACGGGCCATTAATGGGGAGCAATTACAATGCCGAACCCTccac                                                                                                                                                 >  1:489214/1‑225 (MQ=255)
                                                                                   tCAGGAAAAAGGTGGCCTGCACATTACGCTTGATTCCAGCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCcgc                                                                                                                                           <  1:479666/228‑1 (MQ=255)
                                                                                           aaGAGCGCCTGCACATTTCGCTTGATTCCAGCGGTTAAGCCGTTGTCTGTATTGATATGGCGATGAAAATTTCCTTTATGAATCCAGTGGCGGAGAAGATGTGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGc                                                                                                                                   <  1:71643/228‑1 (MQ=255)
                                                                                           aaGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCCGTGGCGGGAAAGATGAGCGGCTGGAAGCAGGAAGAAGCGTTAGGTGTTCCCCTCCTTGCGGGGTTGCAGATTACTTTTTGCGACAACGGACCATTACTGGAGGACATTTACAGTTCCGACCCCCCAcactacccgataccgc                                                                                                                                   >  1:464469/1‑213 (MQ=255)
                                                                                                   cTGCCCATTACGGTTGATTCCATCGGTTAAGGCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTAGGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGAtg                                                                                                                             <  1:438579/226‑1 (MQ=255)
                                                                                                   cTGCATATTCCGCTTTATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTAGGAAGCCAGTGGCGGAGAAGATGGGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGAtg                                                                                                                             <  1:481668/226‑1 (MQ=255)
                                                                                                                                  ccGTGTTTTGTTTTGATATGGGGATGAAAAATACCTTTATGAATCCAGTGGCGGAGAAGATGAGGGGCTGGACGCAGGAAGAAGGGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTacg                                                                                            <  1:611484/228‑1 (MQ=255)
                                                                                                                                     ttgTCTGTTTTGATATGGCGATGAAAATTTCCTTTAGGAAATCAGTGGCGGGGAAGGTGGGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTacgacg                                                                                         <  1:96415/226‑1 (MQ=255)
                                                                                                                                       gtctgtttttgaatggggcttgaaaTTACCGTTATGAAGTCAGGGGCGGAGAAAGTGAGCGGCTGGCAGCCGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTg                                                                                       <  1:44030/206‑1 (MQ=255)
                                                                                                                                       ggttggatatgatggcggttGAAAATTGACTTTATGAATCCAGGGGGGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTg                                                                                       <  1:529937/209‑1 (MQ=255)
                                                                                                                                        tCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTg                                                                                       <  2:434049/227‑1 (MQ=255)
                                                                                                                                              ttGATATGGCGATGAAAATTCCCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTa                                                                                  <  2:505210/226‑1 (MQ=255)
                                                                                                                                              ttGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTTCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTa                                                                                  <  1:291121/226‑1 (MQ=255)
                                                                                                                                              ttGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTa                                                                                  <  2:489214/226‑1 (MQ=255)
                                                                                                                                                 atatGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGGGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGc                                                                                                                                                                                                >  2:153243/1‑113 (MQ=255)
                                                                                                                                                  tatGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCg                                                                                                                                                                                               <  1:153243/113‑1 (MQ=255)
                                                                                                                                                    tgggtataaaaaTTACCTTTATGGATCCAGGGGCGGGGAAGATTGGCCGTTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCGTGACGGTGTTGCATATTCCTTTGGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTa                                                                          <  1:133217/220‑1 (MQ=255)
                                                                                                                                                      gagATGAAATTTTCCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACg                                                                        <  1:282066/226‑1 (MQ=255)
                                                                                                                                                      gCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTTCAGTGCCGCCACCTCCCGTTCCGCGATCGGGCAAGATATGGTGTTGACATTTCGGCGCGGGGGCAGGTTCAACGTGCATAATAGAATTAc                                                                         >  1:34215/1‑227 (MQ=255)
                                                                                                                                                      gCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGGGTTCCGCTCCTGACGGGGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGGGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGGGCAAGATGTGGGGTTGCACTGTCGGAGCGCCGGCAGTTtcgcgctgccttcaagatttac                                                                         >  2:366618/1‑208 (MQ=255)
                                                                                                                                                      gCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGAGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGCCACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAACGGAGGCAGTTACGACGTGCCTTACAGtcattc                                                                         >  2:495717/1‑222 (MQ=255)
                                                                                                                                                      gCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAAGAAAAAGCGTTAGGGGTTCCGCTCCTGGCGGGGTTGCATATTACTTTTTGCGACAACGGGCCATTAATGGAGAACATTTACAGTGCCGGCACCTCACGTTGCGCGATCGAGAAAGAGGTGGGGTTGGACTGGCGGAGCGGGGGAGGTTacacggcgcagtacagagatac                                                                         >  1:624742/1‑207 (MQ=255)
                                                                                                                                                              aaTTTCCTTTTGGAATCCCGGGGCGGAGAAGAGGGGCGGCGGGGCGCAGGAAGAAGCGTTAGGGGTTCCGCTCCTGACGGTGTTGCATATTTCTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAg                                                                <  1:366618/228‑1 (MQ=255)
                                                                                                                                                                       tATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGa                                                       <  2:296251/228‑1 (MQ=255)
                                                                                                                                                                                        ggaataggggggggtGGACGGAGGCAGGAGCGTTAGGGTTTCCGGTCCTGACGGTGTTGCATATTGCTTTTGGCGGCAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTggtt                                      <  1:506086/214‑1 (MQ=255)
                                                                                                                                                                                          aagaTGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTggtt                                      <  2:89774/226‑1 (MQ=255)
                                                                                                                                                                                                        gACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGGCGTGCATTAAAGTATTACGCCGTTAAGTACCCTGGACGGCAGCAATATTTGTTCGGTTCTGGTGGTTCa                      >  1:500709/1‑228 (MQ=255)
                                                                                                                                                                                                        gACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCAACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGGTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCACAATTgagtgcgttctgtcgcttcc                      >  2:136089/1‑209 (MQ=255)
                                                                                                                                                                                                            gaGGAAGTAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCAATAATGGAGAACATTTACAGTGCCGACGCCTCACGGTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGa                   <  2:138079/226‑1 (MQ=255)
                                                                                                                                                                                                                          aGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGCTGTGGTGTTGCACTGTCGGAGCGGCGCCCGTTACGACGTGCCTTCCAGTATTACGCCGTTAAGTACTCTGGCCTGCCGCAATCTTGGTTCGGTTCTTGTGATTCAGGCCGTTACCGAATCAc     >  2:221538/1‑227 (MQ=255)
                                                                                                                                                                                                                            gtgtTCCGCTCCTGACGGTGTTGCATATTACTTTGGGCGACAACGGACCATTAATGGAGAACATTTGCAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCGGGACGTTACCGAATCACGCa  <  2:326143/228‑1 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                                    
CTGAATAAAGAAGGCGAAGTCGAACGTCTCCTCGGCATCAATATGGATATGACCGAAGTGAAACAGCTTAACGAGGCATTGTTTCAGGAAAAAGAGCGCCTGCACATTACGCTTGATTCCATCGGTGAAGCCGTGGTCTGTATTGATATGGCGATGAAAATTACCTTTATGAATCCAGTGGCGGAGAAGATGAGCGGCTGGACGCAGGAAGAAGCGTTAGGTGTTCCGCTCCTGACGGTGTTGCATATTACTTTTGGCGACAACGGACCATTAATGGAGAACATTTACAGTGCCGACACCTCACGTTCCGCGATCGAGCAAGATGTGGTGTTGCACTGTCGGAGCGGCGGCAGTTACGACGTGCATTACAGTATTACGCCGTTAAGTACTCTGGACGGCAGCAATATTGGTTCGGTTCTGGTGATTCAGGACGTTACCGAATCACGCA  >  NC_000913/2144835‑2145282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: