| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,149,958 | T→C | 34.7% | E343G (GAG→GGG) | yegI ← | protein kinase‑related putative non‑specific DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,149,958 | 0 | T | C | 34.7% | 30.8 / 3.3 | 25 | E343G (GAG→GGG) | yegI | protein kinase‑related putative non‑specific DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (3/13); new base C (7/2); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.95e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAT > NC_000913/2149739‑2150163 | cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCCACTGTCGCCCTCACCACCGCCCCAACTTTCGCCAGCCCCAAATTCCCG‑CCGGCGGTTATAACCCCCTCGccc > 2:486493/1‑227 (MQ=255) gcgtGAAGGCCGAAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACGGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATa < 2:20122/225‑1 (MQ=255) ccTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCCACTGGCGCCCTCACCATCGCCCCGAACTTTGCCCGACCAAAATTTCCC‑CCGGGGGGAATGACCCCTTccccccgttcacta > 2:633893/1‑217 (MQ=255) taaaGGCCGAAAAGCAGCAGCAAAAATGCTGAAAGGGGGTTCTAAAGCAAAAGGATGATTCCGCGCCGAAACAGGCCTAA‑AGGAAGGGGCCTGCCAGGCGGTTGGAAATGATCGGTTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa < 2:137902/225‑1 (MQ=255) cagcGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCCACTGACCCCATCACCATCGCCCCGACTTTCGCCAGCCACAAATTCCCC‑CCCGGGGGAAGGACCCCCTTCCCGCGACCAATAAAATAAATAaaacacttata > 1:659438/1‑221 (MQ=255) aGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATTACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCC‑CCGGTGGGAATGACCTCTTCCCCGGGATCAATAAAAAAGGAAAAACCtgtctcttttacaaatttccga > 2:9306/1‑207 (MQ=255) aGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGGGCTACCTACGCCATCACCAACCCCCAGACTTTCACCCGCACAAAATTTCCG‑CCGGGGGGAATGACCCCTTCGCCCGGATCAATAAAAAAGATAAAACCCTTATTTTTCAGCCCAcacccacggcagacaagg > 1:655252/1‑214 (MQ=255) tgatgTATTCGCGCCGTAACAGGCCTAA‑GGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTGGCAATGCTGGCGGTGCTACGGACGCCATCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 2:86614/228‑1 (MQ=255) tgaggTTTTCGCGCCTTAACGGGCGTAA‑AGGAAGCGGCCGGCCAGTCGGTTGGAAAGGATCGGGTAATGGCAGTTGCAATGGTGGCGGTGCTACGGACGCCACCACCATCGCCCAGACTTTCGCCAGCAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 2:127331/228‑1 (MQ=255) tgagtgttcagagcGTAAAAGGCCCAAGAGGGAGCGCCCTGCCAGGGGGTGGAAAATGACGGGGTAATGGCATTTGCGATGTGGGCGGTGCTCCTGACCCCATCACCCCCGCCAGGTCTTCCGCCAGCAAAAATTTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAg < 1:372019/215‑1 (MQ=255) ggttttgtggcgcCGTAACAGGCCTAA‑AGGAAGCGGCCGGCCAGTCGTTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGGGGTGCTACTGACGCCATCACCATCGCCCAGACTTCCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGc < 2:167344/219‑1 (MQ=255) ccGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCCTCGCCCAGACCTTTGCCCGCACAAAAATCCCG‑CCCGGGGGAATGACCTCTTCGCCCAGATCAATAAAAAAAGAAAAACCCTGTTTTACCAGCGCACACCACCGGGCGA‑CCATCAAAAAAGCGGGGGAAACaac > 1:565792/1‑226 (MQ=255) tAACAGGCCAAA‑AGGAAGCGGCCTGCCGGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa < 2:354793/159‑1 (MQ=255) gacggggCCTGCCGGTCGGTTGGAAATGATCGGGAAAGGGCAGTTGAAAGGCTGGGGGTGCAACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc < 1:216940/223‑1 (MQ=255) gcgcgccctgcgtggggTTGGAAATGACGGGGTAATGGCGGTGGCAAGGCTGTGGGGCCTACGGCCCCATTCACCATCCCCCAGACTGTCGCAGGAAAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt < 1:633893/213‑1 (MQ=255) aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGCGCTCCTGACCCCCCCCCCATCCCCCCAACATTCGCCCGCACAAAATTCCCC‑CCGGGGGGAATGACCCCCTCGCCGCGGTCAACAAAAAAAAAAAAACCCCGCTTATCCCGCCCCCCCCACCGGCAATCCAGACAAA‑GATCCGGGGAAACAAGCAATGcccacaacac > 1:136864/1‑215 (MQ=255) tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGAAGCCATCACCATCCCCCAGACGTTCGCCCGCACAAAAATTCCC‑CCGGTGGTAATGACCCCCTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCACCGCCCACCCCAGGCCGG‑CAGTCAAAAAAAAGGGGGAAAAATTAATTGCCGAAAACAAACATTTT‑TTTTAcatgtgggggagaaaagcc > 1:423868/1‑210 (MQ=255) ggtgctgtgcggttacagtcgcggCTGTGCTATGGCCGCTATCCCCCTCGCCCAGACTTCCGCCAACAAAAATTCCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCa < 1:14099/205‑1 (MQ=255) aGTTGCAAGGCTGGCGTTGCTACTGCCGCCATCACCATCGCCCAGACTGTCGCCAGCCAAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTAcc < 2:229356/228‑1 (MQ=255) gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGGGGTAATGACCCCTTCGCCGAGGTCAAAAAAAAAAATAACACCTTGATTATCCCACGCCCACCACGGGCAGA‑CAGGCAAAAAAGCGGGGGAAAAATGCCTTGCTGAAACGGTAACTTTCTTTTAATTTGTGGCTAAGAGAGTCC‑TCGCGGCACCCCACCCTTTAGCCCTCgg > 1:375351/1‑227 (MQ=255) tCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCTTGaagt > 2:349581/1‑226 (MQ=255) aaaTTCCCG‑CCGGTGGTAATGACCCCTTCGCCGAGATCAATAAAATAGATAACACCGTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAAc < 2:136864/228‑1 (MQ=255) aaatCCCGCCCGGTGGTAATGACCTCTTAGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAAc < 2:655252/225‑1 (MQ=255) aTTCCCG‑GCGGTGGTAATGACCCTTGCGGCGAGATCAATAAAATAGATAACCACTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACGGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAt < 2:295944/228‑1 (MQ=255) aTTCCCG‑GCGGGTGTATAGCCCTTCTCGCCGGGAACAATAAAATAGATAACCCCTGGATTAACCAGGGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACAAGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAt < 1:486493/228‑1 (MQ=255) | CGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAAGCAGAATGATGTATTCGCGCCGTAACAGGCCTAA‑AGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCG‑CCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGT‑CAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTC‑TTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAT > NC_000913/2149739‑2150163 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |