Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,782,276 G→C 6.8% A150G (GCG→GGG)  ypjA ← adhesin‑like autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,782,2760GC 6.8% 238.8 / 2.0 82A150G (GCG→GGG) ypjAadhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (40/34);  new base C (4/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTA  >  NC_000913/2782049‑2782503
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
gtgtttccaccgcggctttttaatatTCTCGTTGCTCCCCGTACTTTTTAAGGGTTGGTTTTTCTGCTTTGTTCTGGTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTCCCACCATCAATGAGTATTCCATTCg                                                                                                                                                                                                                                     <  1:214809/206‑1 (MQ=255)
     tCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCTTCCGCTTGCCCTTGAACTTTTACTATTTGGTTACAACCACCAATGAGTTTTCCATTCCCAccc                                                                                                                                                                                                                                >  1:6391/1‑226 (MQ=255)
     tCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCActc                                                                                                                                                                                                                                >  1:345869/1‑228 (MQ=255)
           gcACGGCTCTTATTCACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCACAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTccctccct                                                                                                                                                                                                                         <  2:310934/228‑1 (MQ=255)
                          tctttttGCCCACCGTCATTTTTAATGGTTGTTGTTTCTACTGTTCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCa                                                                                                                                                                                                           <  2:491935/224‑1 (MQ=255)
                           ctctTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTCCTACCTGGCTACCCCCACCAATGAGCATTCCATTCGCACTCCCTCCCTCTCCGCTTGaatccc                                                                                                                                                                                                          >  2:643439/1‑224 (MQ=255)
                            tttttGCTCACCGACATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCAc                                                                                                                                                                                                          <  2:607810/225‑1 (MQ=255)
                              tttGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGCATTCAATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGc                                                                                                                                                                                                       >  2:140311/1‑228 (MQ=255)
                              tttGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGAATTCCATTCGc                                                                                                                                                                                                                                    >  2:306627/1‑199 (MQ=255)
                              tttGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCAGGCCCTTGAACTTTTATTATCTGGCTACCACCATCAATTAGGATTCCATTCCCCCCCCCTCCCCCTAccactgaacccccgc                                                                                                                                                                                                       >  1:159127/1‑214 (MQ=255)
                               tttcTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCa                                                                                                                                                                                                                                   <  1:306627/196‑1 (MQ=255)
                                tGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACTTCCTGCGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATTAGTATTCCATTCGCACTCCTTCCCTCTAcccccaaacacccccc                                                                                                                                                                                                      >  1:329409/1‑212 (MQ=255)
                                tGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTCCCATCAGCATTCCCTTGAACTTTTATTATCTGGCAACCACCATCAATGAGTTTTCCATTCGACATCCTTCCCTCTaaccatcatgaccctcc                                                                                                                                                                                                      >  1:152990/1‑211 (MQ=255)
                                tGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCCCACTCCCTCCCTCTACGCGTGAAGCACCGccc                                                                                                                                                                                                     >  2:49313/1‑228 (MQ=255)
                                      ccGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGAATTCCATTCGCACTCCCTCCCTCTACCCGTGAAGCACCGCCCTTaaat                                                                                                                                                                                               >  1:398357/1‑226 (MQ=255)
                                               ttAATTGTTTTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCa                                                                                                                                                                                      <  2:398357/228‑1 (MQ=255)
                                                tAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGGCCACCATTTATGGTTGTTGTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTCCTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCAt                                                                                                                                                                                     <  2:483074/228‑1 (MQ=255)
                                                        gttgttTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGACCTTTTACTATCTGGCTACCACCATCAATGAGTATGCCATCACCACTCCCTCCCTCTCCCCTTGAATCACCACCCTTATTTTCCCTTCCATTGCtcaca                                                                                                                                                                             >  1:52970/1‑224 (MQ=255)
                                                          tgttTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATAcc                                                                                                                                                                           <  2:345869/228‑1 (MQ=255)
                                                           ttttCTACTGTGTCCTGTTCAACATACTTTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTAGCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATAccc                                                                                                                                                                          <  1:32677/227‑1 (MQ=255)
                                                                tACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGCCTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGCCTACCACCATCAATGAGTATTCCCTTCGCACTCCCTCCCTCTACGCGTGAACAACCGCCCTTAATTGTCGTTcacttacgtagcaccccttt                                                                                                                                                                     >  1:583549/1‑209 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTTTACTACGTCCTGAGAGCCAGACTTATTTATCGTTCTTCCATCACCTTGCCCTTGAACTTTTCCTTTCTGGCTACCACAATCAATGAGTATTCCATTTGCACTCCCTCCCACTACTCGTGCAGACCCGACCTTAATGTGCTTTCCATCCTTCATCCCCCCTTTTTaa                                                                                                                                                                 >  1:21505/1‑227 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTCCCACCATCAATAAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTTTCTTTCCATTGCGATTACCCCCCTTTTaa                                                                                                                                                                 >  1:132801/1‑227 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCACTGATTATTCCATTCGCACTCCCTCCCTCTACCCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGTTACCCCCTTTATaa                                                                                                                                                                 >  2:321268/1‑227 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGACGCACCCCCCTTAATTGTCGTTCCATTGCTGTAACCCCCTTTATaa                                                                                                                                                                 >  1:635907/1‑227 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATaa                                                                                                                                                                 >  2:166268/1‑227 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGAATTCCATTCGCACTCCCTCCCCTTACCCTTCACGCACCGCCCTTATTTGTCGTTCCTTTACTGATACCCCCTTtaaa                                                                                                                                                                  >  1:399446/1‑224 (MQ=255)
                                                                     tgCTCTGTTCAACATACTGTAGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTAGTACTACGTACTGAGAGCCAGTATTATTTATCGTTGTACCATCATACTGGCCTTGACATTTTACTATCTGGCTACAACCATCAATGAGTATACCAGTCTAACTCCCTCCCTCTCCCCGCGAAGCACCGCCCTTAATTGTCGTTCCATTGCTCTTACCCACTTTATaa                                                                                                                                                                 >  1:118557/1‑227 (MQ=255)
                                                                      gCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGATAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCt                                                                                                                                                                       <  2:399446/220‑1 (MQ=255)
                                                                       ctctGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTCATTGTCGTTCCATTGCTGATACCCCCTTTATAAAc                                                                                                                                                               >  2:221147/1‑227 (MQ=255)
                                                                                     ttGTCGTCCACCATTTGTGGTTGTGTTCTTTGCCAGCCTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTTCCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCCCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCAct                                                                                                                                                <  1:49313/227‑1 (MQ=255)
                                                                                     cTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGGACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCAct                                                                                                                                                <  2:124702/228‑1 (MQ=255)
                                                                                      tGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTTTCGTTCCATTGCTGATACCCCCTTTAAAAACGTCCTGATTGCCActc                                                                                                                                               >  1:286941/1‑228 (MQ=255)
                                                                                      tGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGTCGTTACCCCCTTTAAAAACGTCTCGATTGCCActc                                                                                                                                               >  1:182828/1‑228 (MQ=255)
                                                                                                caTTTATGGTTGTGTTCGTTGCCAGACTGCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGAACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCCTCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCAACGCCCTTAATTTTGGTTCCATTGCTGTTACCCCCTTTATAAACGCCCTGCTTGCCACTCCAGATTATCg                                                                                                                                     >  1:658601/1‑228 (MQ=255)
                                                                                                  tttATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTTATACCCCCTTTTAAAACGTCATTTTTGCCAACCTCGATTGTCgga                                                                                                                                   >  1:615790/1‑226 (MQ=255)
                                                                                                    ttgtgtttttttCGTTGCCAGACTTCCTTGTCCTACGTCCTGAAGTCCAACCTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTCCTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTAcc                                                                                                                                 <  1:140647/218‑1 (MQ=255)
                                                                                                      tgggtgttgtCGTTTCCAGACTTACTTGTACTACGTCCTGAATGCTAGACTTATTTATCGTTGGACCATCAGCATGCCCTTTAACTTTTCCTATCTGGCTCCCTCCATCAATGAGTATTCCATTCGCTCTCCCTCCCTCTTCGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCtg                                                                                                                               <  2:638184/219‑1 (MQ=255)
                                                                                                      tGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAAGTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCtg                                                                                                                               <  1:429309/228‑1 (MQ=255)
                                                                                                          tgtgTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAACCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTgg                                                                                                                            >  2:543707/1‑227 (MQ=255)
                                                                                                           gtgtTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGCCCTGATTGCCACTCTCGATTGTCGTACCtgtgtgg                                                                                                                          >  1:424719/1‑225 (MQ=255)
                                                                                                              ttCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGGCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTTACGCACCGCCCTTCATTTTCTTTCCCTTGCTGATACCACCTTTTTAAAAGTCCTAATTGCCACTCCCGCtttttcgccctgtggcaaac                                                                                                                       >  1:458689/1‑210 (MQ=255)
                                                                                                              ttCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGTTTGTCGTACCTGTGGAAATa                                                                                                                       >  2:542976/1‑228 (MQ=255)
                                                                                                                 gTTGCCAGACTTCCTTGTACTACGTCCTGAGCGCCAGGCTTATTTACCGTTGTACAATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCAGTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccc                                                                                                                    <  2:52970/228‑1 (MQ=255)
                                                                                                                 gTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccc                                                                                                                    <  2:635907/228‑1 (MQ=255)
                                                                                                                 gTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATAccc                                                                                                                    <  2:132801/228‑1 (MQ=255)
                                                                                                                   tGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGGACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAGATAccccc                                                                                                                  <  2:424719/228‑1 (MQ=255)
                                                                                                                        gACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTAGCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCa                                                                                                              <  2:583549/227‑1 (MQ=255)
                                                                                                                               ttGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTAGCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGa                                                                                                      <  2:483088/228‑1 (MQ=255)
                                                                                                                                       cttcttGAGAGCCACACTTTTTTATCGTTGTACCATCAGCCTGCCCTTGAACTTTTTCTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCGACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGc                                                                                              <  1:166268/223‑1 (MQ=255)
                                                                                                                                       cGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATTTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAACCGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTgcatgt                                                                                                  >  1:155458/1‑219 (MQ=255)
                                                                                                                                       cGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGc                                                                                                                                                                                                            >  1:42088/1‑118 (MQ=255)
                                                                                                                                       cGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTCATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGACCTGATTGCCACTCTCGATGTTCGTACCCGTGGAAATACCCCCGTCCTGACTTCACTTTCttc                                                                                              >  1:327427/1‑226 (MQ=255)
                                                                                                                                       cGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGcc                                                                                                                                                   >  1:251444/1‑175 (MQ=255)
                                                                                                                                       cGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTAGTACCTACGGAAATAACCCCGTCATGAATTGACTGTCTGc                                                                                              >  1:137687/1‑228 (MQ=255)
                                                                                                                                        gTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCa                                                                                                                                                                                                           <  2:42088/118‑1 (MQ=255)
                                                                                                                                        gTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCa                                                                                                                                                  <  2:251444/175‑1 (MQ=255)
                                                                                                                                         tCCTGAGGTCCAGAATTTTTTATCGTTGTACCTTCAGCATGCCCTTGAACTTTTTCTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCa                                                                                            <  1:643439/228‑1 (MQ=255)
                                                                                                                                            tGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTCCCTGTGTAAATACCCCCGTCATGAATTGACTGCTTGCCAACg                                                                                         >  1:70319/1‑228 (MQ=255)
                                                                                                                                                atccAGTCTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTCCTATCTGGCTACCACCACCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                     <  1:221147/226‑1 (MQ=255)
                                                                                                                                                agCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCTTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTaa                                                                                     <  2:159127/228‑1 (MQ=255)
                                                                                                                                                      tcTTTTTTATCGTTGGACCATCAGCATGCCCTTGATCTTTTACTATCTGGCGACCACCATCAATGAGGATTCCATTCGCACTCCCTCCATCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCTTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGTAATGCCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                               <  1:632571/227‑1 (MQ=255)
                                                                                                                                                      gttttttttttgTTGTCCCATCAGCATGCCCTTTAACTTTTCCTATCTTGCTACCACCATCAATGACTAATCCATTCGCACTCCCTCCCTCTACGCGTGAAACACCGCCCTTAATTGTCGTTCCATTGCTGATCCCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTg                                                                               <  1:140311/217‑1 (MQ=255)
                                                                                                                                                                  ttGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGAACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTTTAACCGTCCTGATTGCCACTCTCAATTGTCGAACCTGTGGAAATACCCCCGCCTTGAATTGCCTGTCTTCCACCGTTAATGttttatttataatcct                                                                   >  2:263689/1‑215 (MQ=255)
                                                                                                                                                                          tCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCTTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTGGACTTATTAGCCTGCCCcaca                                                           >  2:534124/1‑228 (MQ=255)
                                                                                                                                                                             gtaTGCCCTTGAACTTTTCCCAGCTGGCTCCCCCCATCAATGATTATTCCATTCGCACTCCCTCCCTCCACGCGTGAACCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACaaaa                                                        <  1:511974/226‑1 (MQ=255)
                                                                                                                                                                                 tcccTTGTACTTTTTCTATCTTGCTACCCCCGTCAATTATTCTCCCCTTCCCACTCCCTCCCTCTACCCGTGAAGCACCGCCCCTAATTGTCGTTCCATTGCTGATACCCCCTTTAGAAACGTCCTGATTGCCCCTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGt                                                    <  1:534124/227‑1 (MQ=255)
                                                                                                                                                                                   ccTTGATCTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                  <  2:615790/228‑1 (MQ=255)
                                                                                                                                                                                   ccTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTa                                                  <  2:327427/228‑1 (MQ=255)
                                                                                                                                                                                       gAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                              <  2:286941/228‑1 (MQ=255)
                                                                                                                                                                                        aaCTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGACATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTATCCTGCCCCCCAAAATTGTTATGACt                                             >  2:135647/1‑228 (MQ=255)
                                                                                                                                                                                          cTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGAc                                              <  2:6391/225‑1 (MQ=255)
                                                                                                                                                                                                       gCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCAACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGat                               >  2:346170/1‑227 (MQ=255)
                                                                                                                                                                                                                ctCAATGAGTATTCCATTCCCCCTCCCTCCCTTCACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGa                     <  1:321268/227‑1 (MQ=255)
                                                                                                                                                                                                                 tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatt                                                                           >  2:43858/1‑173 (MQ=255)
                                                                                                                                                                                                                 tCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTTTATCTTGATTTCAACCAGa                     >  2:58501/1‑227 (MQ=255)
                                                                                                                                                                                                                  cAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGtatta                                                                          <  1:43858/173‑1 (MQ=255)
                                                                                                                                                                                                                           ttCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTCATGGTTGTATTATTAGCCTGCCCCACAAAATTGtt                                                   >  2:309828/1‑187 (MQ=255)
                                                                                                                                                                                                                           ttCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGAc          <  2:21505/228‑1 (MQ=255)
                                                                                                                                                                                                                            tCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACCAAATTGTTATGACTTCCTATATCTTGATATCCCCCAGATTCAATaaaaa          >  1:252315/1‑224 (MQ=255)
                                                                                                                                                                                                                                ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCTACAGTTTCTGGTTGTATTATTAGCCTGCCCCACCAAATCGTTATGACTTCCTATAGCTTGATATCCACCCACTCCAATAACACTTcc      >  1:233738/1‑227 (MQ=255)
                                                                                                                                                                                                                                ttCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATAGTTATGACTTCCTTAATGTTGCTATCACCCAGTTTCAAAAAAAATTca      >  1:210208/1‑226 (MQ=255)
                                                                                                                                                                                                                                 tCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTCTTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACAAGATTAAATAAAATTTCCa     >  1:393085/1‑227 (MQ=255)
                                                                                                                                                                                                                                   gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATACACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCCTTa  >  2:93741/1‑228 (MQ=255)
                                                                                                                                                                                                                                   gCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCCCCACATTCAAAAAGACATCCATTa  >  1:648324/1‑228 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   
TCGTGTCCAGCGCACGGCTCTCATATACTCTTTGCTCACCGCCATTTTTAATGGTTGTTGTTTCTACTGTGCTCTGTTCAACATACTGTCGACCACCATTTATGGTTGTGTTCGTTGCCAGACTTCCTTGTACTACGTCCTGAGAGCCAGACTTATTTATCGTTGTACCATCAGCATGCCCTTGAACTTTTACTATCTGGCTACCACCATCAATGAGTATTCCATTCGCACTCCCTCCCTCTACGCGTGAAGCACCGCCCTTAATTGTCGTTCCATTGCTGATACCCCCTTTATAAACGTCCTGATTGCCACTCTCGATTGTCGTACCTGTGGAAATACCCCCGTCATGAATTGACTGTCTGCCACCGTTAATGGTTGTATTATTAGCCTGCCCCACAAAATTGTTATGACTTCCTATATCTTGATATCCACCAGATTCAATAAGACTTCCATTA  >  NC_000913/2782049‑2782503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: