Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,849,863	T→G	18.6%	intergenic (‑16/+109)	hycB ← / ← hycA	hydrogenase 3, Fe‑S subunit/regulator of the transcriptional regulator FhlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,863	0	T	G	18.6%	173.6 / 4.7	92	intergenic (‑16/+109)	hycB/hycA	hydrogenase 3, Fe‑S subunit/regulator of the transcriptional regulator FhlA
Reads supporting (aligned to +/- strand):  ref base T (42/28);  new base G (17/5);  total (59/33)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGGTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGA  >  NC_000913/2849638‑2850089                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    ggtggTGATGGCTTTAACCGGGGCGACCCCCGGCGC‑GGGTGTATCTTCCCAGGTGTGGCAGCGCTTCGGCGCTGATTCTTTTTCATTCAGCATCCCCCGCAGGGGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGa                                                                                                                                                                                                                                   <  1:469270/225‑1 (MQ=255)     gTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGt                                                                                                                                                                                                                               >  2:129758/1‑228 (MQ=255)                     ggCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAAc                                                                                                                                                                                                              >  2:225627/1‑228 (MQ=255)                     ggCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGG‑GGC‑AGCCGATACAGAGCGTGGAGGCAGCAATTACAAAACGATTACACAGGCA‑TTCCCCAGGGGAATGT‑CCTTTTTGACGaaaaa                                                                                                                                                                                                              >  1:48850/1‑227 (MQ=255)                      gCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑AATTTTTGACGAAAACa                                                                                                                                                                                                             >  2:649866/1‑228 (MQ=255)                           ccacc‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGT‑GGC‑AGCCGGTACAGAGCGTGGAGTCAGCAATTCCAAAAACATT‑ACCCCGCACACCCCCAGGTGATTGTACAtttttacaaaaaaaagcca                                                                                                                                                                                                         >  1:242914/1‑214 (MQ=255)                               c‑gcgCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCTCTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACACGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCTCCCGGCA‑TTCCTCAGGGAATTGT‑CATTTTTGACGCAAACACGCCGATGa                                                                                                                                                                                                    >  2:523899/1‑228 (MQ=255)                               c‑gcgCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGCGCGTGGAGTCCGCAATTACAAAACGATTCACCAGGCA‑TTCCTCCGGGGATTGT‑CCTTTTTTACGAAAACATGCCGATg                                                                                                                                                                                                     >  1:237758/1‑227 (MQ=255)                                 gcgcAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGACCGTGGAGTCGGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTTACGAAAACAAGCCggagaa                                                                                                                                                                                                   >  1:418407/1‑223 (MQ=255)                                     aGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTAAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGCCGAAAACATGCCGATGAAATga                                                                                                                                                                                               >  2:619175/1‑227 (MQ=255)                                     aGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGT‑GGC‑AGCCGGTACAGAGCGCGGAGTCAGCAATTACAAAACGATTCACCCGGCA‑TTCCCCGAGGATTTGG‑AATTTTTGGCAAAAAAATACCGAAGaaagt                                                                                                                                                                                                >  1:66340/1‑225 (MQ=255)                                       ggTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGG‑CATTTTTGACGAAAACATGCCGATGAAATGTc                                                                                                                                                                                              >  2:496492/1‑227 (MQ=255)                                         tGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTTACGAAAACCTGCCGATGAAATGTCAt                                                                                                                                                                                            >  2:192261/1‑227 (MQ=255)                                              ctgcacaGTGGTGCCAGGGCGGGGGCGAGGATTCTTTTTCATTCAGACTCCCTCTCGGGCGCGGCATTGATTGCCGGGCGGGCGGGCGAGGCGTCTCTGACAAGGCGGCCTCAAAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGa                                                                                                                                                                                      <  1:473586/225‑1 (MQ=255)                                              cTTCACAGGGGGGACAGAGCTGCGGGGCAGATTCTTTTTCATTCAGCATCCCTCTCAGGCGCGGCATTGATTGGAGGCCGTGCTGGGGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGa                                                                                                                                                                                      <  2:180115/228‑1 (MQ=255)                                                 cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACCGAGCGGGAAGTCAGCAATTACCAAACGATTCAACAGGCC‑TTCCGCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCgcaac                                                                                                                                                                                   >  1:426679/1‑224 (MQ=255)                                                 cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTACTCAGTGGATTGT‑CATTTTTGACGAAAACATTCCGATGAAATTCAATTTTGGaca                                                                                                                                                                                    >  2:270889/1‑227 (MQ=255)                                                 cacaGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTAAGCAATTACAAAACGATTCACCAGGCA‑TCCCTCCGGTGATTGT‑CAATTTTGACGAAAACAATCCCCAAAAACGTCATTTTCGacc                                                                                                                                                                                    >  1:162813/1‑226 (MQ=255)                                                      tggtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCac                                                                                                                                                                               >  2:557230/1‑226 (MQ=255)                                                       ggtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCCCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGGGT‑GGC‑AGCCGATACAGAGCGTGGGGTCAGCAATTACAAAACGATTCACCAGGCA‑TCCCTCAGGTGATTGT‑CCTTTTTAACGAAAACATGCCGATGAAATGTCAATTTCGCCCCTCATc                                                                                                                                                                              >  2:395242/1‑227 (MQ=255)                                                        gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGAATGT‑CATTTTTTACGAAAACATGCCGATGAAAAGTCATTTTCGAAACACATCg                                                                                                                                                                             >  1:548944/1‑227 (MQ=255)                                                        gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGATGCAAACATGCCGATGAAATGTCATTTTCGACACTCATcc                                                                                                                                                                             >  2:499117/1‑226 (MQ=255)                                                        gtgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGG‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGCTTACCCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAAAATCCCGAAGAAATGTCATTTTCGAAACTCATcc                                                                                                                                                                             >  1:600090/1‑226 (MQ=255)                                                         tgACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAAAATGCCGATGAAATGTCATTTTCGGCACCCATCGac                                                                                                                                                                           >  2:292688/1‑228 (MQ=255)                                                          ggaagagCTGCGGCGCAGATTCTTTTTCATTCAGCATCCCTCGCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGaca                                                                                                                                                                          <  2:98828/225‑1 (MQ=255)                                                           aCAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCCCAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGCAACTCATCGaaac                                                                                                                                                                         >  1:234472/1‑228 (MQ=255)                                                              ggcgtGCGGCGCAAGTTCTTTTTCATTAGGCCGCACCCGCCGGCGGGGCATTGATTGCAGGCTGTGCTGGCGATTCGTCTCTGAACAGGCGGCCTCACAAGTGG‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGA‑CATTTTCGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGcc                                                                                                                                                                      <  2:187568/224‑1 (MQ=255)                                                                gCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATCCAGAGCGTGGAGTCCGCGATTACAAAACGATTCACCAGGCA‑TTCCTACGGTGATTGT‑CATTTTTTACGAAAACATGCCGGTGCAATGTCATTTTCGacacctactgaacccc                                                                                                                                                                       >  1:187568/1‑213 (MQ=255)                                                                  tGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGCCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAACACATGCCGATGAAATGTCATTTTAGACCCTCATCGACACCCCCCTc                                                                                                                                                                  >  2:561100/1‑228 (MQ=255)                                                                    cggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACCGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTAATTTTCAACACTCATCGCcacccccacccc                                                                                                                                                                >  1:625051/1‑228 (MQ=255)                                                                    cggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGGGTCAGCAATTACCAAACGATTCACCAGGCA‑TTCCTACGGTGATTGT‑CATTTTTGTCGAAAACATACCGATGAAATGTCATTTTTGACACTCATcacaccacccaaccc                                                                                                                                                                >  1:218652/1‑214 (MQ=255)                                                                    cggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTAACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATccc                                                                                                                                                                >  2:264649/1‑228 (MQ=255)                                                                     ggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAACATCCCGATGAAATGTCATTTTCGACACCCATCGACacccccaccccc                                                                                                                                                               >  1:445743/1‑228 (MQ=255)                                                                     ggcgCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGG‑CATTTTTTACGAAAACATGCCCATGAAATGTCCTTTTCGACACCAATCGAAACCCCCAAcccc                                                                                                                                                               >  1:98828/1‑228 (MQ=255)                                                                            aTTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGGAAGGTCATTTTCACCACCCAACGAAACGCCCACCCCCAAACaag                                                                                                                                                        >  1:232664/1‑226 (MQ=255)                                                                                            catccacttcAGGCGGGGCCTTGATTGCGGGCCGTGCTGGCGATGCGGCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCaaaa                                                                                                                                        <  2:442192/220‑1 (MQ=255)                                                                                                  tcacAGGGGCGGCATTGATTGCAGGCCGTGCGGGCGAAGCGTATCTGAAACGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCa                                                                                                                                  <  2:627734/225‑1 (MQ=255)                                                                                                  cctcAGGCGCGTCATTGATGCAAGGCGGGGCTGGCAAAGCGGCTCAGAACAGGCGGCGTAACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCa                                                                                                                                  <  2:626342/227‑1 (MQ=255)                                                                                                       ggCGGGGCATTAGTGGCAGGCCGGGGTGGCGATGGGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGGGGGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc                                                                                                                             <  1:446981/228‑1 (MQ=255)                                                                                                        gcgcGGAATTTATTGCAGGCCGTGCTGTCGAGGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCt                                                                                                                            <  2:334497/228‑1 (MQ=255)                                                                                                        gcgcGGAATGGAGTGCAGGCCGTGGTGGCGATGCGTCTCTGAACAGGCGGCCGCACAAGTTT‑GGA‑CGCCGATACAGAGCGGGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGcc                                                                                                                             <  2:607181/227‑1 (MQ=255)                                                                                                           cgGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAg                                                                                                                         >  2:404092/1‑228 (MQ=255)                                                                                                           cgGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGCAATTTTCGACACTCATCGACACGCCCATCCCCAAAAAGACGTAACGCCTGCAAAACGGGCAAAGCCTCAg                                                                                                                         >  1:198107/1‑228 (MQ=255)                                                                                                            ggCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCCAAACGGGCAAAGCCTc                                                                                                                           >  2:182341/1‑225 (MQ=255)                                                                                                            ggCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTAAAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGG‑CATTTTTGACGAAAACATGCCGATGAAATGCCTTTTTGTAAACTCCTTGACCCCCCCATCCCCAACAAGCAGCAACGCCTGCAAAACGGGAAAAACCCCaca                                                                                                                        >  1:610298/1‑226 (MQ=255)                                                                                                                 gtattgggtgCCTTGCTGGCGAGGCGTCTCGAAACGGGCGGCCTCACAAGT‑T‑GGCAAGCCGATACAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGGA‑TTCCGCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCAtg                                                                                                                   <  1:296966/219‑1 (MQ=255)                                                                                                                      gggggcgggggcggCGATGCGCTTCGGAACAGGCGGCCTCAAAAGTGT‑GGC‑AGCCGATACAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGcc                                                                                                              <  1:496492/216‑1 (MQ=255)                                                                                                                        aGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGG‑CATTTTTGACGAAAACATGCCGATGAAATTTCCTTTTAGACACCCATTGAAACCCCCATCCCCAAAACGGCGGAAAGCCTGCAAAACGGGCaaaacacaaccccaaaccccc                                                                                                              >  1:444794/1‑208 (MQ=255)                                                                                                                          gCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTTGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAACCCCAGCCCactccgcggggc                                                                                                          >  1:299834/1‑217 (MQ=255)                                                                                                                             gTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCCCCTGATGCCggggct                                                                                                        >  1:312115/1‑224 (MQ=255)                                                                                                                                 gggggATGGGTCCCTGAAAAGGCGCGCTCA‑AAATGTGGGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTcc                                                                                                   <  2:584574/224‑1 (MQ=255)                                                                                                                                       ttgtttcttGAACGGGGGGCCCCAAAAGTGG‑GGC‑CGCCGATACAGGGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTAc                                                                                             <  1:619175/220‑1 (MQ=255)                                                                                                                                          gcctccGAACAGGCGGCCCAACAAGTGT‑GGC‑AGCCGATACAGGGCGTGGAGTCAGCAATTAAAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAg                                                                                          <  2:66340/226‑1 (MQ=255)                                                                                                                                           tctcTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATTCCGATGAAATGTCATTTTCGACACTCATCGa                                                                                                                                                                            >  1:631513/1‑145 (MQ=255)                                                                                                                                            ctctGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGGGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGac                                                                                                                                                                           <  2:631513/145‑1 (MQ=255)                                                                                                                                             tctGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGGGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGCCGAAAACATGCCGCTGAAATGTCCTTTTCGACACTCATCGACACGCCAAGCCCCAAACCGCCGTAACACCTGCAAAACCGGCAAACCCTCACCCCCTCCTGCCGGGCTTTTTCCCCTTCCCAGtt                                                                                        >  1:434340/1‑227 (MQ=255)                                                                                                                                             tctGAACAGGCGGCCTAACAAGTGG‑CGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGAGTGT‑CATTTTTGAAGAAAACATGCCGATGAAATGTCATCTTCACCACTCATCGAACGGCCAAACCCCAACAAGGCTTAATCCCGGCACAACGGGCAAAGCCTCAGTCAATCATTGCGGGATTTTTCCCTTTAcccatt                                                                                        >  1:184721/1‑223 (MQ=255)                                                                                                                                                 aaCAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCACCACGCCCATCCCCAAACAGGCGTACAGCCTGCAAAACGGGCAAAGCCTCAGCTCATCCTGCCGGCCTTGTTCCCTTTACCAGTTGGCtt                                                                                   >  2:174365/1‑228 (MQ=255)                                                                                                                                                      gcggcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCCAACAGGCGTAACCCCTGCCACACGGGCAAATCCTCAGCTCATGCTGCCCGGCTTTGTCCCTTTCCCAgacgtctcttat                                                                               >  2:161122/1‑216 (MQ=255)                                                                                                                                                      gcggcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAACCTCAGCCCATGCTGCCGGGCTTGTTCCCTTTACCCGTTGGCTTAAAtt                                                                              >  1:171136/1‑228 (MQ=255)                                                                                                                                                         gcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGCTGAAATGTCATTTTCGACACTCATCGACACCCCCATCCCCAAACAGGCGTAAACCCTGCACAACGGGCCAAGCCTCCGCTCATGCTGCCGGGCTTTAGCCCTTTACCATTTGCCTTAAAATccc                                                                           >  1:380100/1‑226 (MQ=255)                                                                                                                                                         gcCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCTACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGATGGCTTAAATTCAc                                                                           >  1:324197/1‑228 (MQ=255)                                                                                                                                                             cacaAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGCCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGc                                                                       >  2:153810/1‑228 (MQ=255)                                                                                                                                                                 aaggggggC‑AGCCGATTCAGGGCGTGGAGTCAGCAATTACAAAACGATTCCCCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGcaca                                                                    <  2:445743/222‑1 (MQ=255)                                                                                                                                                                   gttgggC‑AACCGATACCGCGGGGGGAGTCAGCAATTACAAAACGATCCCCCAGGCA‑TTCCTCAGGTGATGGC‑CATTTTTGACCAAAACATGCCGATGAAATGTCATGTTCGACAACCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACAt                                                                   <  1:371581/223‑1 (MQ=255)                                                                                                                                                                    gggggC‑AGTCGATGCAGATGGGGGAGTCAGAAATTACAAATCCATTCCCCAGGAA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACAtt                                                                  <  2:560838/224‑1 (MQ=255)                                                                                                                                                                     t‑gGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCCCATTGtt                                                               >  2:77265/1‑228 (MQ=255)                                                                                                                                                                        gC‑AGCCGATACAGAGCGTGGAGTCAGAAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTATTCTAAACACCCATCCCCAACCAGCCGAACAGCAGGCAAAACGGCTAAAACCCACGCCCACGATACCGGGCTTTTTCCCTTTACAAGTTGCTTTAAATTCACCGTCACATGGtttc                                                             >  1:126357/1‑227 (MQ=255)                                                                                                                                                                               gatacgtggCGTGTAGTCAGCAATTACAAAACGATTCCCCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg                                                       <  2:242914/220‑1 (MQ=255)                                                                                                                                                                                atccggagggtggggttacacaTTAAAAAACGATTCCCCAGTCC‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACCGGCCGATGAAATGTCATGTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc                                                      <  1:254415/207‑1 (MQ=255)                                                                                                                                                                                 tccAGAGCGTGTGGTGAGCAATTACAAAACGATTCGCCGGGCA‑TGCCTCAGGTGATTTT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCAGCGACACGCCCATCCCCAAACAGGCTTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCg                                                     <  1:404092/226‑1 (MQ=255)                                                                                                                                                                                 tataaggcttGGAGTCAGAAATTAAAAAACGATTCCCCAGGCT‑TTCCTCGGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg                                                       <  1:192261/217‑1 (MQ=255)                                                                                                                                                                                 tacaggggggggggccaccAATTAAAAAAACATTCCGCGAGCA‑TTCCTCCGGGTATTGT‑CATTTTTGACGAAAACCTTCCGATGAAATGTCATTTTCGACACTCAGCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc                                                      <  1:77265/209‑1 (MQ=255)                                                                                                                                                                                 tACTGAGCGGGGAGTCAACAATTACAAAACGATTCACCCGGCA‑TTCCTCAGGTTATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGGCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc                                                      <  1:129758/227‑1 (MQ=255)                                                                                                                                                                                 tACAGGGTGTGGATTCCGCAATTACAAACAGATTCCCCAGGCA‑TTCCTCAGGGTATTGT‑CATTTTTGACGAAAACATGCCGATGAACTGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAg                                                       <  2:312115/226‑1 (MQ=255)                                                                                                                                                                                 tACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGTTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGc                                                      <  2:234472/227‑1 (MQ=255)                                                                                                                                                                                        cGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGtata                                              >  1:179395/1‑228 (MQ=255)                                                                                                                                                                                        cGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGtat                                               >  2:477526/1‑227 (MQ=255)                                                                                                                                                                                          tGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGc                                                                                                                              >  2:223967/1‑146 (MQ=255)                                                                                                                                                                                          tGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCtttt                                                                                                      >  2:544241/1‑169 (MQ=255)                                                                                                                                                                                           ggAGTCAGGAATTACAAAACGTTCCCCCGGGCT‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAg                                            <  2:162813/227‑1 (MQ=255)                                                                                                                                                                                           ggAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACCTTGTTTTCAATAGCGGTGTATAAg                                            >  2:241865/1‑227 (MQ=255)                                                                                                                                                                                           ggAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCCCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGt                                                                                                     <  1:544241/170‑1 (MQ=255)                                                                                                                                                                                                  gCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCCGTTGGCTTAAATTCACCGGGCCATTGTTTTTAATACCggggacaaagctggcgaa                                    >  1:582970/1‑212 (MQ=255)                                                                                                                                                                                                         cAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCATTAGCGGCGTCTAAACTGTCGTAAACGCCa                             >  2:100108/1‑228 (MQ=255)                                                                                                                                                                                                                 ttCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTTTAAGCTGTCGTAAACGCCAGCGAtttt                     >  2:56721/1‑228 (MQ=255)                                                                                                                                                                                                                 ttCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACCCCAGCGAattt                     >  1:39689/1‑228 (MQ=255)                                                                                                                                                                                                                        ggCA‑TTCCCCAGGTTATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCgaga              <  2:548944/228‑1 (MQ=255)                                                                                                                                                                                                                           a‑TTCCTCAGGGTATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAg           <  2:179395/228‑1 (MQ=255)                                                                                                                                                                                                                             gtCCTCAGGGTGTTGA‑CATTTTGGACGAAAACATGCCGATGAAATGGCATTTTCGACCCTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAgt          <  2:380100/227‑1 (MQ=255)                                                                                                                                                                                                                                cTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCCGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAATGtt       >  1:531625/1‑228 (MQ=255)                                                                                                                                                                                                                                     gTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGCTTTTTTCGAGATAGGGTTcccaa  >  1:358374/1‑225 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    GCGGGTGATGGCGTTAACCGGGCAGACCACC‑GCGCAGGGTGCATCTTCACAGTGGTGACAGAGCTGCGGCGCAGATTCTTTTTCATTCAGCATCACTCTCAGGCGCGGCATTGATTGCAGGCCGTGCTGGCGATGCGTCTCTGAACAGGCGGCCTCACAAGTGT‑GGC‑AGCCGATACAGAGCGTGGAGTCAGCAATTACAAAACGATTCACCAGGCA‑TTCCTCAGGTGATTGT‑CATTTTTGACGAAAACATGCCGATGAAATGTCATTTTCGACACTCATCGACACGCCCATCCCCAAACAGGCGTAACGCCTGCAAAACGGGCAAAGCCTCAGCTCATGCTGCCGGGCTTTGTCCCTTTACCAGTTGGCTTAAATTCACCGGCACATTGTTTTCAATAGCGGTGTATAAGCTGTCGTAAACGCCAGCGATTTTTTCGAGATAGTGTTCCAGA  >  NC_000913/2849638‑2850089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑