Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,053,513 T→C 21.4% S2G (AGC→GGC) 
*442W (TGA→TGG
ubiH ←
pepP ←
2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
proline aminopeptidase P II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,053,5130TC21.4% 75.2 / 2.3 45S2G (AGC→GGC) 
*442W (TGA→TGG
ubiH
pepP
2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
proline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (13/21);  new base C (9/2);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 1.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAT  >  NC_000913/3053290‑3053739
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     
aTGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGCCTTAACCGGGAAATAGCCAGAGCCCGCGTCGCGCCCGCCCTGCCGCCACCGACCATGATTACGCCCAtt                                                                                                                                                                                                                                   >  1:115327/1‑228 (MQ=255)
aTGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAACGCCAGCGCCGGGCCCGCCATGCCGCCACCGACGATGATTACGCTCAt                                                                                                                                                                                                                                    >  2:183737/1‑227 (MQ=255)
     aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCCTGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATCCCGCCACCGACGATGATTACCCCCCTTGCtt                                                                                                                                                                                                                               >  1:414270/1‑227 (MQ=255)
     aGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATACCCACCGCCAGCGCCCCGCCCGCCCTGCCCCCACCCGCGGTAATTACCCCCAttcgct                                                                                                                                                                                                                               >  1:104070/1‑223 (MQ=255)
       ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGCTGATTACGCTCCATGCGTtc                                                                                                                                                                                                                             >  2:226932/1‑227 (MQ=255)
       ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGAATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGCCTTAACCGGGAACTAGCCAGCGCCAGCGTCGCGCCCGCCATGCCCCCACCGACCATGATTACGCCCATTGCTTtct                                                                                                                                                                                                                            >  2:95320/1‑228 (MQ=255)
               tCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGCCTTAACCGGGAAATAGCAAGCGCCAGCGTCGCGCCCGCCATGCCGCCCCCGCCGATGATTACGCTCATTGCTTTCCCccagca                                                                                                                                                                                                                    >  2:314571/1‑227 (MQ=255)
                           tGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATCCCGCCACCGCCGATGATTACCCCCCTTGCTTTCTCGCACCAccccccacccctt                                                                                                                                                                                                       >  1:658106/1‑218 (MQ=255)
                                       aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTcc                                                                                                                                                                                            >  2:520606/1‑227 (MQ=255)
                                       aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAACAACCCTCAACGCCTCGGTTACTTCCg                                                                                                                                                                                           >  2:282698/1‑228 (MQ=255)
                                                       gACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCCGCCCGGCCCTGCCTCCACCGCCGATGGTTCAGCCCATTCCTTTTCGCCGCCAACCACCAACCCTTCGATTTCTTCCGTCTGTTCCCCCACACt                                                                                                                                                                           >  1:5883/1‑228 (MQ=255)
                                                                    cgccAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGCCGCGCCCGCCCTGCCCCCACCGACGATGATTCCGCCCATAGCTTTCTCTCAGCAACCCTCAACGCTTCCATTTATTCCCGCGTTTTCACCACGCTGACGGGGAggggc                                                                                                                                                              >  1:373984/1‑225 (MQ=255)
                                                                              aTCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCCCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGTTTTCGTTACCg                                                                                                                                                     >  2:366675/1‑227 (MQ=255)
                                                                                 ctttctCCCTCAAAACCCGGGTGGAGCGTGGGACTGGGGCGCAGGCGCTTAAATCAAGTGGCCGTGCAGCCCCCCGTGACTGAACCGGAAAATGGCCACCGCCAGCCTCGACCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGtt                                                                                                                                                  <  1:314571/223‑1 (MQ=255)
                                                                                    cGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGAAAGGGTTTAGTTAACGGTTTTgg                                                                                                                                              >  1:558851/1‑228 (MQ=255)
                                                                                            aaaGCCGGGAGGAGCCTGTGACTCGGGCGCCGTCGCTTCAATCAAATGGCCCGGCCGCCCCCCGTGAATTAACCGGGAAATAGCAAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATcaca                                                                                                                                     <  2:610398/228‑1 (MQ=255)
                                                                                                 cccgggtggggaggtcgctcgGGCGCAGCCCCTTCAATCAAATGCACCGGCAGCGCCCGAGTGCTGAACCGGGAAATAGCCAGCCCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgt                                                                                                                                 <  1:330268/211‑1 (MQ=255)
                                                                                                    gATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGCCGGTGAGGTTTTCTGTTCCGGTTTTGGTAATCACAATtttgtc                                                                                                                             >  2:405398/1‑228 (MQ=255)
                                                                                                                     tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACAACGCTGGCGGTGAGGGTTTCTTTTCCCGTTTCTGGAATCACACAGTTGGCTTCCATTAGAATTCCg                                                                                                             >  2:644964/1‑228 (MQ=255)
                                                                                                                     tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCACCAACGCCTCGATTTCTTCCGGCTTTTTCACCCCGCTGGCGGCGTGGTTTTCGTTTCCGGGTTCGGATACGCACAAGTCGGCTTCAAACCAAATGcca                                                                                                             >  1:7477/1‑227 (MQ=255)
                                                                                                                       gcggAGTCTCTTCAACAAAATGGCCGGGAGCCCCCCGGTGCTCTAACCGGGAAAAGCCCGGGCCCAGCGTCGGCCCCGCCATGCCGCCACCGGCGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGa                                                                                                            <  1:95320/227‑1 (MQ=255)
                                                                                                                       gcctgatCGCTTAAATCAATGGTCCCGGAAGCGCCCCGTGATTTAACGGGGAACTCCCCAGCGCCCGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGa                                                                                                            <  2:364864/221‑1 (MQ=255)
                                                                                                                             tCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGTTTTCTTTTCCGTGTTCGGTACTACCAATATCGTCTTCATAACAAAAGCCggcaacccg                                                                                                     >  1:572965/1‑220 (MQ=255)
                                                                                                                                          actggccgggccgcccccGGCACTTACCCGGGAAATGCCCAGGCCCTGGTCTGGGCCCGCCATGCCGCCCCCGACGATGATTACGCTCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg                                                                                        <  1:76896/213‑1 (MQ=255)
                                                                                                                                          aTGTCCCGGAAGCCCCCGGGGACTTAACCGGAAAATGCCCAGCGCCAGGTGCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg                                                                                        <  2:115327/227‑1 (MQ=255)
                                                                                                                                                       gcCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTCTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCCAGACCGCGATATTGTTTTTGCACTTTTTCATCCg                                                                          >  2:418894/1‑228 (MQ=255)
                                                                                                                                                        ccccGTGACTTAACCGGGAAATAGCCAGCGCCCGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCgg                                                                         <  2:414270/228‑1 (MQ=255)
                                                                                                                                                            cggTGACTGAACAGGGAATTACCCAGCGCCGGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGATCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgcg                                                                       <  2:19642/226‑1 (MQ=255)
                                                                                                                                                                   ttcacccggaaaacggccgcgcccagcgTCCCCCCCGCCATCCCCCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCGTCGACGGTTCGATTTGTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTGCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGTCGCAatat                                                                 <  1:183737/206‑1 (MQ=255)
                                                                                                                                                                        cGGGAAATGGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGATTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc                                                           <  2:104070/228‑1 (MQ=255)
                                                                                                                                                                           gAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGGTTTCGTTAACGGGTTCCGTTATCCCAAATTCGGCTTCCATACGAATGCCCATACCGCCAAAATGTTCTGGGACTTCTGCATCCCgccccaaaaaaaagcacg                                                        >  1:222791/1‑212 (MQ=255)
                                                                                                                                                                                ccgccgcgcccgccTCCCGCCCGCCATGCCGCCACCGCCGATGATTACGCTCATTGCTTTCGCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                                   <  2:592296/223‑1 (MQ=255)
                                                                                                                                                                                 gcccccgcccgcgTCGCCCCCCGCCATGCCCGCACCGATGCTGTTTACCCTCAGTGCTTTCCCGCAGGACCCATAAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                                   <  1:282698/223‑1 (MQ=255)
                                                                                                                                                                                     gcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGCGAGGTTTTCGTTTCCGGTTTCGGCAATCACAACATCGTTTTCAAACCTAAAGCCGATACCCGCTATTTTTTTTTGGACCTTCTCATCCCCCGCAataaaaaacacacgcgctacag                                               >  1:554626/1‑208 (MQ=255)
                                                                                                                                                                                        gccccggccccccccccgccATGCCGCCACCGACGATGATTCCGCTCCTTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGt                                              <  1:418894/214‑1 (MQ=255)
                                                                                                                                                                                         ccccagcgccggcgccgccATGCCGCCACCGACGGTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGt                                              <  2:90364/213‑1 (MQ=255)
                                                                                                                                                                                          ccctaggtgccccccgccATGCCGCCACCGACGATGATGACGCTCATTGCTTTCCCGCATCAACCATCAACTCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACgggc                                             <  2:485584/215‑3 (MQ=255)
                                                                                                                                                                                                    cgcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTAAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc                                  <  2:558851/228‑1 (MQ=255)
                                                                                                                                                                                                     gcccgtcATGCCGCCACCGACGGTGATTACGCCCATTGCTTTCGCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc                                  <  2:60520/227‑1 (MQ=255)
                                                                                                                                                                                                     ccccggcATGCCGCCACCGACGATGATTACGTTTATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg                                    <  2:339067/224‑1 (MQ=255)
                                                                                                                                                                                                                        gccgttcatTACGCTAATTTCTTTCCCGCGACCACCATCATCGCTTGGATTTCTTCCGGCTTATTCCCCGCGCTGGCTGGGAGGTTTTTGTTGCCGGTGTCGGAAATCACAATGTCGTCGTCGATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCGCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag               <  1:578370/221‑1 (MQ=255)
                                                                                                                                                                                                                                ttACGCCCATTGCTTGTCCGGCGCAACCATTAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTg       <  2:5883/228‑1 (MQ=255)
                                                                                                                                                                                                                                 tccGCTCCTTGCTGTCCCTGAGCAGCCATAACCTCTTGGTTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCCGAATTTCGTCTTCAATACTAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCGAGAATGCGCGAGCGATCCTGa      <  1:478561/226‑1 (MQ=255)
                                                                                                                                                                                                                                     cTCATTGGTTTCCCGCAGCAACCATCCACGCTGCGATTTCGTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAt  <  2:222791/228‑1 (MQ=255)
                                                                                                                                                                                                                                     cTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTaa                                                                                                                                          >  1:525120/1‑93 (MQ=255)
                                                                                                                                                                                                                                      tCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAt                                                                                                                                         <  2:525120/93‑1 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     
ATGGCAGTTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAT  >  NC_000913/3053290‑3053739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: