Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,053,524 T→C 28.3% R439G (AGA→GGA)  pepP ← proline aminopeptidase P II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,053,5240TC28.3% 61.8 / 3.8 42R439G (AGA→GGA) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (13/14);  new base C (3/10);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCAC  >  NC_000913/3053297‑3053749
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    
ttGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGAATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGCCTTAACCGGGAACTAGCCAGCGCCAGCGTCGCGCCCGCCATGCCCCCACCGACCATGATTACGCCCATTGCTTtct                                                                                                                                                                                                                                      >  2:95320/1‑228 (MQ=255)
        tCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGCCTTAACCGGGAAATAGCAAGCGCCAGCGTCGCGCCCGCCATGCCGCCCCCGCCGATGATTACGCTCATTGCTTTCCCccagca                                                                                                                                                                                                                              >  2:314571/1‑227 (MQ=255)
                    tGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATCCCGCCACCGCCGATGATTACCCCCCTTGCTTTCTCGCACCAccccccacccctt                                                                                                                                                                                                                 >  1:658106/1‑218 (MQ=255)
                                aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTcc                                                                                                                                                                                                      >  2:520606/1‑227 (MQ=255)
                                aTGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAACAACCCTCAACGCCTCGGTTACTTCCg                                                                                                                                                                                                     >  2:282698/1‑228 (MQ=255)
                                                gACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCCGCCCGGCCCTGCCTCCACCGCCGATGGTTCAGCCCATTCCTTTTCGCCGCCAACCACCAACCCTTCGATTTCTTCCGTCTGTTCCCCCACACt                                                                                                                                                                                     >  1:5883/1‑228 (MQ=255)
                                                             cgccAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGCCGCGCCCGCCCTGCCCCCACCGACGATGATTCCGCCCATAGCTTTCTCTCAGCAACCCTCAACGCTTCCATTTATTCCCGCGTTTTCACCACGCTGACGGGGAggggc                                                                                                                                                                        >  1:373984/1‑225 (MQ=255)
                                                                       aTCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCCCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGTTTTCGTTACCg                                                                                                                                                               >  2:366675/1‑227 (MQ=255)
                                                                          ctttctCCCTCAAAACCCGGGTGGAGCGTGGGACTGGGGCGCAGGCGCTTAAATCAAGTGGCCGTGCAGCCCCCCGTGACTGAACCGGAAAATGGCCACCGCCAGCCTCGACCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGtt                                                                                                                                                            <  1:314571/223‑1 (MQ=255)
                                                                             cGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCCCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGAAAGGGTTTAGTTAACGGTTTTgg                                                                                                                                                        >  1:558851/1‑228 (MQ=255)
                                                                                     aaaGCCGGGAGGAGCCTGTGACTCGGGCGCCGTCGCTTCAATCAAATGGCCCGGCCGCCCCCCGTGAATTAACCGGGAAATAGCAAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATcaca                                                                                                                                               <  2:610398/228‑1 (MQ=255)
                                                                                          cccgggtggggaggtcgctcgGGCGCAGCCCCTTCAATCAAATGCACCGGCAGCGCCCGAGTGCTGAACCGGGAAATAGCCAGCCCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATgt                                                                                                                                           <  1:330268/211‑1 (MQ=255)
                                                                                             gATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGCCGGTGAGGTTTTCTGTTCCGGTTTTGGTAATCACAATtttgtc                                                                                                                                       >  2:405398/1‑228 (MQ=255)
                                                                                                              tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACAACGCTGGCGGTGAGGGTTTCTTTTCCCGTTTCTGGAATCACACAGTTGGCTTCCATTAGAATTCCg                                                                                                                       >  2:644964/1‑228 (MQ=255)
                                                                                                              tGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCACCAACGCCTCGATTTCTTCCGGCTTTTTCACCCCGCTGGCGGCGTGGTTTTCGTTTCCGGGTTCGGATACGCACAAGTCGGCTTCAAACCAAATGcca                                                                                                                       >  1:7477/1‑227 (MQ=255)
                                                                                                                gcggAGTCTCTTCAACAAAATGGCCGGGAGCCCCCCGGTGCTCTAACCGGGAAAAGCCCGGGCCCAGCGTCGGCCCCGCCATGCCGCCACCGGCGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGa                                                                                                                      <  1:95320/227‑1 (MQ=255)
                                                                                                                gcctgatCGCTTAAATCAATGGTCCCGGAAGCGCCCCGTGATTTAACGGGGAACTCCCCAGCGCCCGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGa                                                                                                                      <  2:364864/221‑1 (MQ=255)
                                                                                                                      tCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGGGGTTTTCTTTTCCGTGTTCGGTACTACCAATATCGTCTTCATAACAAAAGCCggcaacccg                                                                                                               >  1:572965/1‑220 (MQ=255)
                                                                                                                                   actggccgggccgcccccGGCACTTACCCGGGAAATGCCCAGGCCCTGGTCTGGGCCCGCCATGCCGCCCCCGACGATGATTACGCTCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg                                                                                                  <  1:76896/213‑1 (MQ=255)
                                                                                                                                   aTGTCCCGGAAGCCCCCGGGGACTTAACCGGAAAATGCCCAGCGCCAGGTGCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTgg                                                                                                  <  2:115327/227‑1 (MQ=255)
                                                                                                                                                gcCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTTCTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCCAGACCGCGATATTGTTTTTGCACTTTTTCATCCg                                                                                    >  2:418894/1‑228 (MQ=255)
                                                                                                                                                 ccccGTGACTTAACCGGGAAATAGCCAGCGCCCGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCgg                                                                                   <  2:414270/228‑1 (MQ=255)
                                                                                                                                                     cggTGACTGAACAGGGAATTACCCAGCGCCGGCGCCGCGCCCGCCATGCCGCCACCGACGATGATTACGATCATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGgcg                                                                                 <  2:19642/226‑1 (MQ=255)
                                                                                                                                                            ttcacccggaaaacggccgcgcccagcgTCCCCCCCGCCATCCCCCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCGTCGACGGTTCGATTTGTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTGCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGTCGCAatat                                                                           <  1:183737/206‑1 (MQ=255)
                                                                                                                                                                 cGGGAAATGGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGATTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGcc                                                                     <  2:104070/228‑1 (MQ=255)
                                                                                                                                                                    gAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGAGAGGGTTTCGTTAACGGGTTCCGTTATCCCAAATTCGGCTTCCATACGAATGCCCATACCGCCAAAATGTTCTGGGACTTCTGCATCCCgccccaaaaaaaagcacg                                                                  >  1:222791/1‑212 (MQ=255)
                                                                                                                                                                         ccgccgcgcccgccTCCCGCCCGCCATGCCGCCACCGCCGATGATTACGCTCATTGCTTTCGCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                                             <  2:592296/223‑1 (MQ=255)
                                                                                                                                                                          gcccccgcccgcgTCGCCCCCCGCCATGCCCGCACCGATGCTGTTTACCCTCAGTGCTTTCCCGCAGGACCCATAAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGctct                                                             <  1:282698/223‑1 (MQ=255)
                                                                                                                                                                              gcgccagcgTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGCGAGGTTTTCGTTTCCGGTTTCGGCAATCACAACATCGTTTTCAAACCTAAAGCCGATACCCGCTATTTTTTTTTGGACCTTCTCATCCCCCGCAataaaaaacacacgcgctacag                                                         >  1:554626/1‑208 (MQ=255)
                                                                                                                                                                                 gccccggccccccccccgccATGCCGCCACCGACGATGATTCCGCTCCTTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGt                                                        <  1:418894/214‑1 (MQ=255)
                                                                                                                                                                                  ccccagcgccggcgccgccATGCCGCCACCGACGGTGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGt                                                        <  2:90364/213‑1 (MQ=255)
                                                                                                                                                                                   ccctaggtgccccccgccATGCCGCCACCGACGATGATGACGCTCATTGCTTTCCCGCATCAACCATCAACTCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACgggc                                                       <  2:485584/215‑3 (MQ=255)
                                                                                                                                                                                             cgcccgccATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTAAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc                                            <  2:558851/228‑1 (MQ=255)
                                                                                                                                                                                              gcccgtcATGCCGCCACCGACGGTGATTACGCCCATTGCTTTCGCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAGCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGcc                                            <  2:60520/227‑1 (MQ=255)
                                                                                                                                                                                              ccccggcATGCCGCCACCGACGATGATTACGTTTATTGCTTTCCCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATg                                              <  2:339067/224‑1 (MQ=255)
                                                                                                                                                                                                                 gtcgattattcccgctaTTGCTTTCCCGCAGCAACCCTCAACGCTTCGATTTCTTCCGGCTTTTTCCCCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag                         <  1:442011/212‑1 (MQ=255)
                                                                                                                                                                                                                 gccgttcatTACGCTAATTTCTTTCCCGCGACCACCATCATCGCTTGGATTTCTTCCGGCTTATTCCCCGCGCTGGCTGGGAGGTTTTTGTTGCCGGTGTCGGAAATCACAATGTCGTCGTCGATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCGCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCgcgag                         <  1:578370/221‑1 (MQ=255)
                                                                                                                                                                                                                         ttACGCCCATTGCTTGTCCGGCGCAACCATTAACGTTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTg                 <  2:5883/228‑1 (MQ=255)
                                                                                                                                                                                                                          tccGCTCCTTGCTGTCCCTGAGCAGCCATAACCTCTTGGTTTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCCGAATTTCGTCTTCAATACTAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCGAGAATGCGCGAGCGATCCTGa                <  1:478561/226‑1 (MQ=255)
                                                                                                                                                                                                                          tacggccATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTTCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGa                <  2:658106/222‑1 (MQ=255)
                                                                                                                                                                                                                              cTCATTGGTTTCCCGCAGCAACCATCCACGCTGCGATTTCGTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCAt            <  2:222791/228‑1 (MQ=255)
                                                                                                                                                                                                                              cTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTaa                                                                                                                                                    >  1:525120/1‑93 (MQ=255)
                                                                                                                                                                                                                               tCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAAt                                                                                                                                                   <  2:525120/93‑1 (MQ=255)
                                                                                                                                                                                                                                         ctcGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGGTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCAATTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCCAGCCATCCTGACCATAAACACCCAc  >  2:204590/1‑227 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                    
TTGCGCAATCCGCCAGAGATTGCCAGACGCCGATGCGCGCCAGTTGCTGACAGGTACCCGCCGCCAGCGCTATCGCTCGTCCATCAAAGCCCGGATGAGCATGTGACTCTGGCGCAGTCGCTTCAATCAAATGTACCGGCAGCGCCCCGTGACTTAACCGGGAAATAGCCAGCGCCAGCGTCGCGCCCGCCATGCCGCCACCGACGATGATTACGCTCATTGCTTTCTCGCAGCAACCATCAACGCTTCGATTTCTTCCGGCTTTTTCACCACGCTGGCGGTGAGGTTTTCGTTACCGGTTTCGGTAATCACAATGTCGTCTTCAATACGAATGCCGATACCGCGATATTGTTCTGGCACTTCTGCATCCGGCGCAATATACAGCCCTGGCTCTACGGTCAGTACCATGCCCGGTTCCAGAATGCGCGAGCGATCCTGACCATAAACACCCAC  >  NC_000913/3053297‑3053749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: