| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,346,883 | C→T | 6.0% | P103L (CCG→CTG) | ptsN → | sugar‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,346,883 | 0 | C | T | 6.0% | 192.0 / 3.7 | 70 | P103L (CCG→CTG) | ptsN | sugar‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base C (27/37); new base T (2/2); total (31/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA > NC_000913/3346658‑3347106 | aaaaaCGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCgg < 2:121794/228‑1 (MQ=255) gcgcCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGAGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGACGATGGCGTTTTCGTTCAGATGGAAACGACTATAGCTTTCGATGCCCACGAAAACCAACCGGTggca > 2:303027/1‑225 (MQ=255) atcaGCGAGGCGGGGGCGAAACAACGTAGCCTGCCTGCTCAGGTGGTTTTGGAAGCTAT‑CCTGACGCGGGAAAAAAGTGGCAGGACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCctg < 2:39660/228‑1 (MQ=255) atcaGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGCCTTACTTTTTGCCctt > 1:172561/1‑227 (MQ=255) gggcggcGAAAAAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCgg < 2:419443/227‑1 (MQ=255) gcccaaaaaaactAGCCTCCCGCTGCATGTGTTTTTGGACTCTCG‑CCTGGCGCGGGAAAAAATGGGCAGTACCGGTACCGGCAATGGTAGTGCCATTCCGCATGGAAAACTGGAAGAAGATCCTCTGCTCGCCGTTGGCGTGTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCGCTGCTGGTGCCGGCAGa < 1:272594/216‑1 (MQ=255) aaaCACTTTGGCCTGCGGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCa < 2:9338/227‑1 (MQ=255) aGCCGGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTCTTGCCATTCCGACTGGCCCACTGGAAGCCGCTCCTCTGCGCGCCGTTGGCGGTTTCGTTCAGCTCGAACAGCCTCTAGCTTTCGCTGCCATGGACACCCCCCCGGTGGAATTCCTTTTTGCCCTGCCGGTGCCGGCCGACAAAACCaaaaa > 1:45111/1‑226 (MQ=255) gcctgcctCCTCATGTGGTTTTTGACGCTAG‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTTCCGGCAATGGTATTGCCGTTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg < 1:587286/227‑1 (MQ=255) gCCTGCGGCCGTGGGTGGTTGTTGAAGCTAT‑CTTGACACGGGAAAAAATGGGAAGTACCGTTCTCGGCAAGGGTATTGCGATTCCGCATGGCAAACTGGAAGAAGATACGCGGCGCGCCGTGGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTGCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACg < 1:141973/227‑1 (MQ=255) ccTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCCAAACGCCTATAGCTTTCGATGCCACCGACAACCAACAGGCGGACTTCATTTTTGCCCTGCTGGTGCCGGCAGACCAACCTaaaaaacc > 1:609936/1‑224 (MQ=255) ccTGCCGCCTCAGGGGGTTTTTGAAGCTTT‑CCTGGCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 1:489957/228‑1 (MQ=255) ccTGCCGCCTCAGGGGGTTTTCGAAGTCTG‑CCGTACCCGGGAAAAAATGGTCAGCACCGGGATCGGCAATGGTAGTGCTATTCCGCATGGCAAACTGGAAGAAGATATCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 1:351083/228‑1 (MQ=255) ccTGCCCGCTAAGGTGGTTTGTGAAG‑TATACCTGACGGGGGAAAAAATGGGAAGTACCGGTATCGCCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 1:551141/228‑1 (MQ=255) cTGCCGGCTCAGGTGGTTTTTGAAGCGAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTAACGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCa < 2:344917/227‑1 (MQ=255) cgccTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTg > 1:658047/1‑227 (MQ=255) gcctgcggtggtTTTTGAAGCTAT‑ACCGCCGCGGGAAAAAATGGGCAGTACCGGTATCGGCAAGGGTATTGCCATTCCGAATGGCAAACTGGAAGAAGATTCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCa < 1:660023/222‑1 (MQ=255) tcTCAGGTGGTTTTTGAAGGTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCAt < 2:239399/227‑1 (MQ=255) ttttGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGGCAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCt > 2:268866/1‑228 (MQ=255) tAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACAAAACTAAAACGCACCTCCTTACCCTGTCCCtgtgggccaa > 1:87939/1‑220 (MQ=255) gcgggggAAAAAATGGGCAGTACCTGTAGCGGGATTGTTATTGCCGTTCGGCAGTGCAAACTGGAAGAAGATGCCCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg < 1:551580/224‑1 (MQ=255) tgAAAAAATGGGCAGGCCCGGTATCGGCAATGGTAGTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTTCGCGCCGTTGGCGTTTCCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGa < 1:169646/227‑1 (MQ=255) ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGCTACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCAGGTGTCGAAACGGCTGGCgg > 1:347388/1‑227 (MQ=255) ggAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTAGCAACGCCTATAGCTTTCGATGCCATCGACAACCCAACGGTGGACTTACTTTTTGCCCTGCTGGTGCGGGCAGACCAAACCAAAACACACCTCGATACACTTTGGCTGGTGGCGAACAGTCTTGCgg > 1:459644/1‑227 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCACACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgg > 2:651485/1‑227 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGTTGGTGGCGAAACGTCTGGCGGACAAAACCACCTGCCCCCGTTTAcgc > 1:561255/1‑228 (MQ=255) ggCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGGGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCCTCATACTCTGTCGCTGGTGGCGCAAGGTCTGGCGGACAAAACAATCTGCCGCCGTTTAcgc > 1:230422/1‑228 (MQ=255) gCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAACTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg > 1:257766/1‑228 (MQ=255) gtaTGGTTTTGCCTTGCCGCAAGGAAAACTGGAAGGAGATTTGCGGCGAGGCGTTGGCGGTTTCGTTCAGCCCGAAATGCCTATGGCTTTCGAGGCCATCGACAACCAACCGGTGGACGTTCGTTTTGCCCTGCTGGTGCCGGCAGACCAAACTCAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 1:432724/225‑1 (MQ=255) gaaTGGTTTTGTCATTCCGCAGGGCAAACTGGAAGAAGATACTGTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 1:226614/226‑1 (MQ=255) cAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATCCTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 2:105185/227‑1 (MQ=255) aaaTGGTATGGCCATTCCGCAGGGCAAACTGGAAGAAGATTCCCCGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTAcgcg < 2:316976/226‑1 (MQ=255) tttttgcATTCCGCCTGGCAAACTGGAAAAAGTTACTTTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCa < 1:161629/222‑1 (MQ=255) ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGCCCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGGCTGGGGGACAAAACCATCTCCCCCCGTTTACCcccagcccagg > 1:329782/1‑227 (MQ=255) ttGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATATTCCGTCGCTGGTGTCGAAACGTCTGCCGGCCAAAACCATCTGCCGCCGTTTACCCGCAACCCaca > 1:439536/1‑226 (MQ=255) cATTCCGCATGGCAAACTGGAAGAAGATATCCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGGCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTTGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg < 2:230422/227‑1 (MQ=255) ttCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg < 2:329782/228‑1 (MQ=255) tCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGCCAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGTGa > 2:438881/1‑228 (MQ=255) tCCGCAAGGCAAACTGTAAGAAGATACTCTGCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACCGGGGGACTTACTTTTTTCCCTGCTGGGGCCGCACGACCAACCTAAAACGAACCAGACTACTCTATGCCTAGTGGCGAAACACTTGACGGACAAAACAAACTGCCGCCGTTTAACGGCAGCCCAGAGCgggga > 1:442950/1‑224 (MQ=255) ccGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGGGCCACCCAGAGCGagtaa > 2:53028/1‑224 (MQ=255) gaTGGAAAACTGGAAGAAGATACGCGGGGCGCCGTTGGCGTTTTCGTTCAGCTCGAATCGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAagag < 1:438881/227‑1 (MQ=255) cgggCAAAATGGAAAAAGATACCGTTCGCGCGGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCCTCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGc < 1:331376/226‑1 (MQ=255) tGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGAAGAGCt > 2:72437/1‑228 (MQ=255) cAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCAACTGCCGCCGTTTCCGCGACCCCCGGAGCGATAGAGAGCTGt > 1:402464/1‑227 (MQ=255) tGGAAGAAGATACTCTGCGGGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCaaa < 2:389554/228‑1 (MQ=255) gaagagGATTCCCTGGCCGCCGTTGGCGTGTTCGTTCAGCTCGAAACTGCTAATGATTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAAt < 2:261005/227‑1 (MQ=255) tctGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTCTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGGAGACGAGATGAATCAAATACTTACgagtaa > 1:73641/1‑223 (MQ=255) tGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAt > 1:423725/1‑197 (MQ=255) gcgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATg < 2:423725/197‑1 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGc > 2:32957/1‑192 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGTCGGACAAAACCATCTGCCGCCGTTTACGCGCCACCCCGAGCCGTGAAAAGCTGAATCAAAACATTCCCGAAACCGaa > 1:248965/1‑228 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCGGAGCGATGAAAAGCTGTATCAAATCATTACGGATACCGaa > 2:121907/1‑228 (MQ=255) cgcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGac > 2:549190/1‑227 (MQ=255) gcgcCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGTCAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCg < 1:32957/192‑1 (MQ=255) gTTGGCCTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACt < 2:55919/228‑1 (MQ=255) ttCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACCAAACCATCTGCCGCCGTTTACCCGCAGCCCAGAGCGATGAAGAGCTGTATCCAATCATTACGGATACCGAATGGACTCCGGATaaa > 1:401749/1‑228 (MQ=255) tCGTTCAGCTCGAAACGCCTTTAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAg < 1:557753/228‑1 (MQ=255) cTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACa > 2:616779/1‑123 (MQ=255) tataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACaa < 1:616779/123‑1 (MQ=255) ttaGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 2:347388/226‑1 (MQ=255) attgCTTTCGCTGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTTCATACTCTGCCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 2:520404/224‑1 (MQ=255) ataGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGt < 2:257766/227‑1 (MQ=255) taGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGtct < 2:405191/228‑1 (MQ=255) ttCGATGCCATCGACACCCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGAAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGAACTCCGGATGAAGCGTCGTTATTCGGTAAGGTCTCtttt > 2:187907/1‑228 (MQ=255) tCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGTTACCGCAGGGACTCCGGATGAACCGCAGTTATTCGGTAATGTATCTTTTa > 1:83119/1‑228 (MQ=255) tgATGCCCGCGACCACCAACCGGTGGCACTACGTTTTGCCCTGCTGGCGCCGGCAGACCAAACTAAAACGCACCTGCATGCGCTGTCGCTGGTGGCGAAACGTCGGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGAGGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAg < 1:392818/227‑1 (MQ=255) cATCGACAACCAACGGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGTTGACGAGCTGTATCAAATCCTTACGGATACCGAAGGAACTCCGGAGGAAACGTAGTTTTTCGGAACTTTCTCCTTTaagctgtg > 1:361847/1‑221 (MQ=255) cGACAACCAACTGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTtgtg < 2:73641/227‑1 (MQ=255) gcaaccaacCGGTGGACTTCCTTTTTGCCCTGCTGGGGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg < 2:432318/227‑1 (MQ=255) acaacaAACCGGTTGCCTTTCTTTTGGCCCGGGTGGGGCCGGCCGGCCAAACTAAAACGCACCTGCATACTCGGGCGCTGGTGGGGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATTATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTgagg < 1:268866/228‑1 (MQ=255) caacgGGGGGACTTTCTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa < 1:549190/228‑1 (MQ=255) aaccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGGCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGaaa < 2:658047/227‑1 (MQ=255) accGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCt > 2:501998/1‑87 (MQ=255) ccGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTg < 1:501998/87‑1 (MQ=255) | AAAAACGCGCCCTGGAAATCATCAGCGAGCTGGCGGCGAAACAACTTAGCCTGCCGCCTCAGGTGGTTTTTGAAGCTAT‑CCTGACGCGGGAAAAAATGGGCAGTACCGGTATCGGCAATGGTATTGCCATTCCGCATGGCAAACTGGAAGAAGATACTCTGCGCGCCGTTGGCGTTTTCGTTCAGCTCGAAACGCCTATAGCTTTCGATGCCATCGACAACCAACCGGTGGACTTACTTTTTGCCCTGCTGGTGCCGGCAGACCAAACTAAAACGCACCTGCATACTCTGTCGCTGGTGGCGAAACGTCTGGCGGACAAAACCATCTGCCGCCGTTTACGCGCAGCCCAGAGCGATGAAGAGCTGTATCAAATCATTACGGATACCGAAGGTACTCCGGATGAAGCGTAGTTATTCGGTAATGTCTCTTTTAGACGTTGTGAGGAGAAA > NC_000913/3346658‑3347106 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |