Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,638,184 A→G 26.0% S182G (AGC→GGC)  pitA → phosphate transporter, low‑affinity, tellurite importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,638,1840AG26.0% 70.8 / 5.6 54S182G (AGC→GGC) pitAphosphate transporter, low‑affinity, tellurite importer
Reads supporting (aligned to +/- strand):  ref base A (15/22);  new base G (8/7);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTACCTGGTACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACT  >  NC_000913/3637968‑3638408
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
gctacctgtgtattgggTTTACCTGCATCCAGCTTTCATACGATGATTGGCGCGAGCATCGGGATTGGATGAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAg                                                                                                                                                                                                                               <  2:207746/213‑1 (MQ=255)
          gtgttGGTTTACTTTACTTCAGTTCTAATACGCTGAGTGGCGCGCTCGTCGGGATTCGTTTAACCAATGCGTTGATGGCCGGGACGTCAGTGGTGGATTCAGTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGccc                                                                                                                                                                                                                    <  1:74427/225‑1 (MQ=255)
              tGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTTGCGGTCTGATTTTCTTGCTTCCTCGCTTCTGGAGCGGCACCCAGAAACGCGCCCGTa                                                                                                                                                                                                                >  2:367613/1‑227 (MQ=255)
                     ccTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTTGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCt                                                                                                                                                                                                         <  2:430878/227‑1 (MQ=255)
                     ccTGCATCCAGCGTTCATGCGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCt                                                                                                                                                                                                         <  2:197251/227‑1 (MQ=255)
                     ccTGCAGCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCt                                                                                                                                                                                                         <  2:19985/227‑1 (MQ=255)
                      cTGCATCCAGCTCTTATCAGGTAGTTGGGCCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGa                                                                                                                                                                                                       <  2:127659/228‑1 (MQ=255)
                           tCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGGGTTTGCTGGCGGTCTGATTTTTTTTCTGCCTCGCGCCTGGGGCGGCACCCAGAAACACGCCCCTTTCccccgctcccca                                                                                                                                                                                                  >  1:653129/1‑220 (MQ=255)
                               gCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGATTTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTTCTCCGCTACTGGGAGTGCCACACTAAACGCGCCCGTATCGCCCTGACCCCAGCgg                                                                                                                                                                                              >  2:183946/1‑228 (MQ=255)
                                     aTACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCCATTGTCGGCCTGGGGTTTGCTGGCGGGTTGGTTTTTTTTTTGCGTCGCTCATGGGGCGGCACCACGACCGGCGCTTTTATCCATCTGAcgcctccgccgagcg                                                                                                                                                                                        >  1:382590/1‑217 (MQ=255)
                                      tgcGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAg                                                                                                                                                                                                                                         <  2:382590/178‑1 (MQ=255)
                                             aTTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTTTTTCCCCGTTCCCACTTGATCGGCACCAAGAAAAGCGCCCCTACCCCCCTGACCCCCACGGGGCGGGAaaaggaa                                                                                                                                                                               >  1:463877/1‑228 (MQ=255)
                                               tGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCGATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGGGTTTGCTGGCGGTCTGATTTTCTTTCTGCGTCGCTCCTGGGGGGGGCACAAGAAACGCGCCCGTATCCACCCGACCCCCCCGGGGGGGGAAaaaaaaga                                                                                                                                                                             >  1:619236/1‑228 (MQ=255)
                                                                     taaacaaagggtgtgatgaCGGGGCGGGTAGTGGGGGATCCCCTCAAATCCCCTGAAGTATTAAGTTTTTCCGGTTCTTGGATCTTTTCCCCGTGTGGCGGCCGGGGGTTTGCTGGGGTGCGAATTTTCTTGCTGCGTCGCTACGGGGGCGGCACCAAGAAGCGCGCCCGTACCCACCGGACCCAAGGGGGGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTc                                                                                                                                                      <  1:397977/215‑1 (MQ=255)
                                                                           aatggtttaatggCCGGGACGGTGGGGGTGGATGCCCTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGAc                                                                                                                                                 <  2:338532/218‑1 (MQ=255)
                                                                           aagggtgtgatgaCCGGGACGGCCGGGGTGGAAGCACTCAATTTCCCGAAAGTATTAAGTATTTCCGGTTCCCTGATCGTTTCCCCTATTGTCGGCCGGTTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGAc                                                                                                                                                 <  2:110958/220‑1 (MQ=255)
                                                                             tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGGAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTTTTGCTTCGTCGCTACTGGGGCGGCCCCAAGAAACGGGCCCCTATTCACCTGACCCCACCGGAGCGTGCAAAGAAAGACGGCAAGAAAAAACCGCCCTTCtcggccc                                                                                                                                               >  2:34107/1‑221 (MQ=255)
                                                                             tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGGTTTTCTTGCTGCCTCGCTCCTTGCGCGGCACCAAGAAACTCGCCCCGTTCCCCCCGCCCCCAACGGAGCGGCGAAAAAAAAACGGGAAGAAAAAaccacccctccgggccgg                                                                                                                                              >  1:601671/1‑216 (MQ=255)
                                                                             tGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCCGCGGGGGGTGAAAAGAAAGACGGCAAGACAAAGCCTCCCtt                                                                                                                                                       >  2:397977/1‑219 (MQ=255)
                                                                                  tgatgaCCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTTTTTCTGCGTCGCTACTTGGACGGCACCAAAAAAAGCGCCCTTTTTCACCTGCCCCCCGCGGCGCGTGAAAAACAAAACGGACAGAAAAAACCGCCGGTATggggccgaattt                                                                                                                                         >  1:502264/1‑218 (MQ=255)
                                                                                         cGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCCCTACTGGAGCGGCACCAAGAAACGCGCCCGGATCCACCCGCACCCAGCGGAGCGTTGAAAGAAAGACGGCAAGAAAAAACCGCCGTTCTGGACCCGCAtttggcggat                                                                                                                                  >  2:206869/1‑220 (MQ=255)
                                                                                              cGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTTTTGCCGCGTTGCTACTGGGGGGGGACCAAGGAAAGCGCCCGGTTTCCCCCTGCACCAACGGAGCGTGCAAAAAAAAACGGCCAGAAAAACCCCGCCGACTGGCCGCGGATTGCGCTGAGCCtgt                                                                                                                             >  1:473422/1‑226 (MQ=255)
                                                                                               ttCAGTGGTATATGTACTCCATTCCACGAAAGTATTAGTTATTTGCGTTTCTCTGATCGTCTCCCCTGTTGTCGGCCTGTTTTTTGCTGGCGGTCTGATTTTCTTGCTGCGGCCCATCTGGGCCGGCACCAAGAAACGCGCCGGTTTCCACCTGACCCCAGCGGGGCGTGAAAAAAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCttgc                                                                                                                            <  1:256084/227‑3 (MQ=255)
                                                                                                     agggccagCACTCAATACCCGGAAAGTATTAAGGATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCGTGGGGTTTGCTGGCGGTCGGATTTTCTTGCTGCGTCGCTACTGGAGCGGACCCAAGAAACGCGCCCGTACCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTa                                                                                                                       <  2:60072/220‑1 (MQ=255)
                                                                                                                    tatCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGGATCCACCTGACCCCAGCGGAGCGGGAAAAGAAAGAAGGCAAGAAAAAACCGCCGTTCTGGACGCGACATGCGCTGATCCTTTCGGCTATCGGCGTGGGGgtttt                                                                                                     >  2:578429/1‑227 (MQ=255)
                                                                                                                       cccGAAAGTTTTAAGGTTTTCGGGTTCTCTGCTCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCGTGCTGCGTCGCTACGGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCa                                                                                                  <  2:247596/228‑1 (MQ=255)
                                                                                                                        cgggatgttttgatttttttcggttttcggACGGTTTCCCCTGTGTCGGGCCGGGTGTTTGCTGGCGGTCTTATTTGTTTGATGCGTCGCTGCTGGGGCGGCACCAAAAAACGCGCCCGTATCCACCTGACCCGAGCGGAGTGTAAAAAGAAAAACGGCAAGAAAACGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCAc                                                                                                 <  1:367613/212‑1 (MQ=255)
                                                                                                                         cGAAAGTATTAATTATTTTCGGTTCTCTGATCGTTTCCCCTATTGCCGGCCTGGTGTTTGCTGGGGGTCTGATTTTCTTGCTGCGTCGCTACGGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCAc                                                                                                 <  2:619236/227‑1 (MQ=255)
                                                                                                                          gAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTTCTGCGTCGCTACTGGAGCgg                                                                                                                                                                                                                                      >  1:367228/1‑99 (MQ=255)
                                                                                                                           aaaGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGc                                                                                                                                                                                                                                     <  2:367228/99‑1 (MQ=255)
                                                                                                                            aagtattaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTTGTTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAACAAACCGCCGTTCTGGACGCGTCTTGAGCTGAACCTTGCCGCTATCGGCGGGGCGTTTTTGccccgac                                                                                             >  1:445393/1‑222 (MQ=255)
                                                                                                                            aagtattaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGGGCGTGAAAAGAAAGACGGCAAGAAAAAACCGCCGTCTTGGCCGCGTTTTGCGCTGATCCTTTCCGCAATCGGCGTTGCGGTTTTGCACcgcg                                                                                             >  1:143293/1‑228 (MQ=255)
                                                                                                                                 ttaagtattTTCGGTTCTCTGATCGTTTCCCCTATTGTCGTCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTAATGGTGCGGCACCAAGAAACGCGCCCGTTTTCCCCTGACCCCCGCGGAGCGTGATAAGCAAGAAGGCCAGAAAAACACGCCCCTCTGGACCCCGATTGCCCTGGTTCTTTCCGCCACTCGGCGGGCCTtttttccccgcgcggac                                                                                         >  1:420012/1‑215 (MQ=255)
                                                                                                                                         tttCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTTCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATACACCTGACCCCAGCGGAGGGTGAAAAGAAAGACGGCCAGAAAAAACCGCCGTTCTCGACCCGGATTGCGCTGTTCACTTCCGCCATCGGAGTGGGCGTTTTccccggccgcgaccactgtca                                                                                 >  1:409137/1‑208 (MQ=255)
                                                                                                                                         tttCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGGGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAAACGGCCAGAAAAAGCCCCCGTTCTGGACGCGGCTTTGGCCGAACCCTTCCGCCATCGGCCTGGGGGTTTGCGCCCGGGGGGAACGAAggcg                                                                                >  1:132693/1‑226 (MQ=255)
                                                                                                                                         tttCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACCCGCATTGCGCTGATCCTTTCCCCTATCGGCGTCGCGTTTTCTCACCGGGCGGACACTGGTcc                                                                               >  2:488332/1‑227 (MQ=255)
                                                                                                                                         tttCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACCTGGGCGGCCCCCAGAAACGCGCCCGTTTCCCCCTTACCCCCCCGGGGCGTGAAAAGAAAGACGGCCAGAAAAAACCCGCGGTCTGGACACCGATTGCGCGTATCCTTTCCCCCATCGCGGTGGGGTTTTTGccgcgcgcccacaagggtca                                                                                >  1:588956/1‑209 (MQ=255)
                                                                                                                                           tcggttctcgttgtgTTTCCCTTAGTGTCGGCCTGGTTGTGGCGGGCGGTTCGGTTTGCGTGCTGCTTGCTTACTGGGGCGGCACAAATGAACGCGCCTGGTCCCACCTGCCCCCAGAGGAGCGTAAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGa                                                                            <  1:509163/214‑1 (MQ=19)
                                                                                                                                                tctcTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCCGCGGAGCGTGGAAAGAAAGACGGCAAGAAAAAGCCGCCCGTCTGGACGCGTATTGCGCTGATCCTTTCCCCTATAGGCGTGGCGTTTTCTGACgggccggaacaggtgcaaaaaagc                                                                         >  1:457517/1‑206 (MQ=255)
                                                                                                                                                 tctggaccttttCCCCTGTGTGCGGCCGGGTGTTTGCTGGCGGTCTGATTTTCGTGCTGCGTCGCTACGGGAGCGGCACAAAGAAACGCGCCGGTATCCACCTGACCCAAGGGGAGCGGGAAAAGAAAGCCGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCa                                                                      <  1:488332/219‑1 (MQ=37)
                                                                                                                                                   ctGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTAACCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGAc                                                                                                                                                 <  2:38057/155‑1 (MQ=255)
                                                                                                                                                     gtttgTTTCCCCTTTTGTGGGCGTGGTGTTTGCGGGCGGTTGTATTTTCGTGCTGGGCCGCTAGGGGGGCGGCCACAAAAAACGCGCCCGTATCCACCGGCCCCCAGCGGGGGTTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTgg                                                                  <  1:614542/224‑1 (MQ=25)
                                                                                                                                                      tttgTTCCCCCTTTTGTCGGGGTTGTGTTTGCTGGCGGTCTGATTTTGTTGCTGCGGCGCTACTGGAGCGGCACAAAGAAACGCGCCCGTACCCCCCTGCCCCCAGCGGAGTGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGt                                                                 <  1:540536/225‑1 (MQ=38)
                                                                                                                                                      tatgttttccctgtttccgggCCGGGTGTTTGCTGGCTGTCTGTTTTTTTTGCTGCGTCCCTACTGGAGCGGCACCAAAAAACGCTCCCGTACCCACCTGACCCCGGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTgg                                                                  <  1:131728/209‑1 (MQ=37)
                                                                                                                                                      aTCTTTTCCCCGTTTGGCGGCCTGGTTTTGGGTGGCGGCTGGATTTCTTTGCGCGGTCGCTCCTGGGGGGGCACCAGAAAACGGGCCGATATCCACCGGACCCCAGGGGGGGTGGAAAAGAAAAACGGCAAGAAAAGGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGt                                                                 <  1:206869/228‑1 (MQ=19)
                                                                                                                                                       ttgTTTCCCCTATTGGCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGGGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGt                                                                 <  2:558355/225‑1 (MQ=255)
                                                                                                                                                        atgtgtataagagacgtgcccTGGTGTTTGCTGTCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTc                                                                <  2:154064/209‑1 (MQ=255)
                                                                                                                                                            tCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAACCCCCGGTCTGGACGCCGATTGCGCTGATCCTTTCCGCCATCGGCGTGGGGGTTTTCCCCGGCGCGACCGACGGGCAGAAAAGCacttgggtggt                                                            >  1:558355/1‑217 (MQ=255)
                                                                                                                                                                         ggCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGGGTTTTTTCACGGCCCGCACGATGGTCCGAAAAGGCTTTGGCTGGGTTTGTTGGGattt                                               >  2:204376/1‑227 (MQ=255)
                                                                                                                                                                                     gCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCCAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGGGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTCGACGCGGATTGCGCTGATCCTTTCCCCTATCGGCGTGGGCTTTTTGCCCGGGGCGCACGGAGGGTAAGAAAGCAAGGGTCGGGGTCTGTTTGTTATGGTTGGCgcccgg                                   >  1:569551/1‑223 (MQ=255)
                                                                                                                                                                                           gggttgtttttCCTGTTGCGTCGCTATGGGAGCGGCCACAAGAAGCGCGCCCGTCTCCCCCTGACCCGAGGGGGGGGTGAAAAGAAAGACGGCAAGAAAAGGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCcagc                              <  1:418567/220‑1 (MQ=37)
                                                                                                                                                                                                   gtttgtgggcgcgcggacaggggAGGGGCACCAAGAAACGGCCCGGAAACCAGCTGACCCCAGCGGAGGGAAAAAAGAAGGTAGTGAAGAAAAGGCCGCCGGTCGGGACGAGTATTGCGCTGAGCCTTGCCGGGATCGGCGGGGCGTTTTCGCATGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCg                      <  1:461648/207‑1 (MQ=255)
                                                                                                                                                                                                        ttctggggtgCTACGGGGGCGGCAACAAGAAAGGCGCCCTGAACCACCTGACCCCAGGGGGCGTTGAAAAGAAATACGGCAAGAAAAAGGCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCgtggtg                 <  1:34107/218‑1 (MQ=37)
                                                                                                                                                                                                                      tGGAGCGGTCCCAAGAAACGCCGCCTGTCCCACTCGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCTTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACt  <  2:454136/228‑1 (MQ=37)
                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
GGTACCTGGTACTTTGGTTTACCTGCATCCAGCTCTCATACGCTGATTGGCGCGATCATCGGGATTGGTTTAACCAATGCGTTGATGACCGGGACGTCAGTGGTGGATGCACTCAATATCCCGAAAGTATTAAGTATTTTCGGTTCTCTGATCGTTTCCCCTATTGTCGGCCTGGTGTTTGCTGGCGGTCTGATTTTCTTGCTGCGTCGCTACTGGAGCGGCACCAAGAAACGCGCCCGTATCCACCTGACCCCAGCGGAGCGTGAAAAGAAAGACGGCAAGAAAAAGCCGCCGTTCTGGACGCGTATTGCGCTGATCCTTTCCGCTATCGGCGTGGCGTTTTCGCACGGCGCGAACGATGGTCAGAAAGGCATTGGTCTGGTTATGTTGGTATTGATTGGCGTCGCGCCAGCAGGCTTCGTGGTGAACATGAATGCCACT  >  NC_000913/3637968‑3638408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: