Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,765,515 A→G 26.1% S1112G (AGT→GGT)  rhsA → Rhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,765,5150AG26.1% 53.5 / 2.5 54S1112G (AGT→GGT) rhsARhs protein with putative toxin 55 domain, putative polysaccharide synthesis/export protein, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (25/12);  new base G (12/4);  total (37/17)
Fisher's exact test for biased strand distribution p-value = 7.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGC  >  NC_000913/3765296‑3765734
                                                                                                                                                                                                                              |                                                                                                                                                                                                                           
aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCCGTGTGGGGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGCAAGTGAAATCCTGGCTGCCCGGGTGGGGGGGGaa                                                                                                                                                                                                                     >  1:120227/1‑228 (MQ=255)
aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGGGGGGTTCCCGCCGGCGCTGGTGCAGATGCTCGACCGGCGGGGAAGGGAAATCCTGGGTGACGGGGGGGGTggggga                                                                                                                                                                                                                     >  1:579595/1‑226 (MQ=11)
aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGGGTGGTGTTCCCGCCGGTGCTGGGGCAGATGCTCGCCCGGCTGGACAGTGAAATCCTGGCTGCACGGGTGAGGGAGGaa                                                                                                                                                                                                                     >  2:619418/1‑228 (MQ=255)
aaCGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGGGGGGTGTTCCCGCCGGTGCTGGGGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGCCCGGGGGAGGGGGGaa                                                                                                                                                                                                                     >  1:248423/1‑228 (MQ=255)
  cGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGGGTTCCCGCCGGTGCTGGTGCAGCTGCTCGGCCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGGGTGGGGAAAg                                                                                                                                                                                                                   >  2:951/1‑228 (MQ=255)
     cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGGGTGGTGTTCCCGCCGGTGCTGGTGCGGCTGCTCGACCGGCTGGAAAGGGAAACCCCGGCCGGCCGGGGGGGTGAGGAAAgcc                                                                                                                                                                                                                 >  1:382664/1‑227 (MQ=11)
     cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCCTCAGCAGTCCGGCGGCGAAAACGGTGGCCGTGCGGGGTTCCCGCCGGTGCTGGGGCAGATACGTGACCCGCTCGCAAGGGAACCCCTGGGTGACCGGGGGGGTGGGGaaaagc                                                                                                                                                                                                                 >  1:377321/1‑224 (MQ=11)
     cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCCCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGGAATCATGGCTGACCGGGTGAGTGAGGAAAgcc                                                                                                                                                                                                                 >  2:460698/1‑227 (MQ=21)
     cAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCAGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGGGTTCCCGCCGGTGCTTGTGCAGAGGCTCGACCGGCTGGGAAGGGAAATCCAGGCTTACCGGGGGGGGGAGGAAAgccc                                                                                                                                                                                                                >  1:267067/1‑227 (MQ=11)
        aaCCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGGGTGCGGAAAgccgcc                                                                                                                                                                                                              >  2:208097/1‑227 (MQ=21)
             gCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGGGTGGTGTTCCCGCCGGGGCTGGTGCAGATGCTCCACCGGCTGGAAAGGGGAAGCCTGGCTGCCCGGGTGGGGGAGGAAACCCCCGcctggc                                                                                                                                                                                                        >  1:366746/1‑228 (MQ=11)
                         tttATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGAGAGTGAGGAAAGCCGCCGCTTGCTGGCATCGTGCg                                                                                                                                                                                            >  2:399394/1‑228 (MQ=21)
                                 ccGGGGAGCTTCACGCCACTCCTCAGAGTTGAAACCGCTACCGGTGGGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAGGACGGTGGCAGTGTGGGGTTCCCGCCGGTGCTGGTGCAGAGGCTCGTCCGGGTGGGGAGTGAACTCAGGGCTGACCGGGGGGTGGAGGAAAGCCGCACCTGGCGGTCACCGGGCGGcccggcc                                                                                                                                                                                    >  1:515278/1‑223 (MQ=11)
                                         ctcccggcacccatagggagGTGAACCCGCCACGGGGGGGCGGGCAAAAACCGGGCTCCGCTGCTGGGCGGAGGCGCTCCTGCTGCGCGGGGGCGAGGAGGGGGGCGGTGGGGGGTGCCCGCCGGTGCTGGTGGAGAGGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGAAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGc                                                                                                                                                                             <  1:386665/210‑1 (MQ=2)
                                                   cTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGCTGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGGGTGAGGAAAGCCGCCCCTGGCTGGCATCGTGCGGCCTGACCGGGGAGCAGATGCAAAAc                                                                                                                                                                  >  2:454809/1‑228 (MQ=255)
                                                   cTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGGGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGCGCAGATGCAAAAc                                                                                                                                                                  >  2:113341/1‑228 (MQ=25)
                                                      atcaGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCCGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGCGTTCCCGTCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAACTCCTGTCTGACCGGGTGGGTGGGGCAAACCGCCGCAGGGCGGCATCGCGCCGACCGACCCGGGAGCAGATCCAACACCaa                                                                                                                                                               >  1:564144/1‑227 (MQ=11)
                                                           agTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGGGTTCCCGCCGGTGCTGGGGCAGATGCTCGACCGGCTGGAAAGGGAAATCCTGGCTGACCGGGTGGTGGAGGAAAGCCGCCGCTGGCGGGCATGCGGCGGCCTGAACgggggaccaaggcaaaacaaaaagg                                                                                                                                                           >  1:164947/1‑206 (MQ=11)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTTGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCGGACCGTGGAGAAAAGGAAAAACCAAAAGGAc                                                                                                                                                         >  1:187281/1‑227 (MQ=11)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGGAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGAc                                                                                                                                                         >  2:142871/1‑227 (MQ=18)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGAGGAGCAGCTGCAAAACCCGATgggc                                                                                                                                                         >  2:395890/1‑225 (MQ=18)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCATCTGGCGGCCTGACCGGGGGGCAGATGAAAAACAAAAGGGAcc                                                                                                                                                        >  1:596205/1‑228 (MQ=11)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCGGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCCTCGGGCGGCCTGCCCGTGGGGCAGATGCAAAACCAAATgggc                                                                                                                                                         >  2:380737/1‑225 (MQ=11)
                                                             ttGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGGGAAATCCTGGCTGACCGGGTGAGGGAGGAAAGCCGCCGCCGGCTGGCCTCGTGCGGCCCGCACGCGAGGCAGATGCCAAACCAAAGGGaac                                                                                                                                                        >  1:566677/1‑226 (MQ=11)
                                                                aaaCCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGCGAGTGAGGGAAGCCGCCGCTGGCTGGCATCGTGCGTCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCgc                                                                                                                                                     >  2:594268/1‑227 (MQ=17)
                                                                                 cTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGGGAGGAAAGCCGCCGCTGGCTGGCATCTGGCGGCCTGACCGGGGCGCAGATGCCAAACCCAATGGGCCCGGGGCGCACGCGGgcg                                                                                                                                       >  1:106434/1‑225 (MQ=11)
                                                                                    gCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGCGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGAGGCTCGACCGGCTGGCAAGTGAAATCCTGGCTGACCCGGCGAGGGAGGGAAGCCGCCGCTGGCCGGCATAGCGCGGCCTGACCGTGGCGCAGATGCAAGACCCGCTGGACCCGCGGTACACGCCGCCGCGaaa                                                                                                                                  >  1:54504/1‑227 (MQ=11)
                                                                                              aGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGGGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGGGAAAAATCCACCTg                                                                                                                        >  2:614281/1‑227 (MQ=14)
                                                                                                         ggggCGGATGCGCCTCAGCGGGCCGGGGGGAAAGGCGGGGGGTGTTGGGTCTTCCCGCCGGTGCTGGTGCGGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGa                                                                                                             <  2:106434/225‑1 (MQ=11)
                                                                                                                              tCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCCAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCCCTGGcc                                                                                       >  2:166791/1‑228 (MQ=11)
                                                                                                                                        aggagggTGGCGTTGTGGTGTTCCGGCCGGTGCTGGTGCAGATGCCCGCCCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGc                                                                              <  2:187281/222‑1 (MQ=255)
                                                                                                                                          cgcggtggttgttgtttgtgCCCGCTGGTGTGGGTGAAGGTGCGCGGCCGGCTGGAAAGTGAAATCCTGGCTGACCGGGGGAGTGAGAAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAACCCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCTCCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCaag                                                                           <  1:395890/212‑1 (MQ=11)
                                                                                                                                            cGGTGGTAGTTGGGTTTCCCCGCCGGTGCTGGTGCAGATGCTCGCCCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCaagga                                                                         <  2:5195/228‑1 (MQ=255)
                                                                                                                                                ggcggggtggtgTCCCCGCCGGTGCTGTGGCAGAGGCTCGACCGGCTGGAAAGGAAAATCCTGGCTGACCGGGTAAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCaaggaagg                                                                      <  2:120227/221‑1 (MQ=11)
                                                                                                                                                 ggtgttgggtgTTCCCCGCGGTGGTGGTGAAGGTGCTGGACGGGGTGAAAAGGAAACCTCTGGCTGACCGGGTGGTGGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGa                                                                    <  1:544000/221‑1 (MQ=11)
                                                                                                                                                  gtgtgggtggtccccGCCGGTGCTGGTGCAGATGCTCGCCCGGTTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCGGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGa                                                                    <  2:164947/215‑1 (MQ=255)
                                                                                                                                                  gggttgggtcTTCCCGCCGGTGCTGGTGCAGATGCTGGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGa                                                                    <  2:382664/221‑1 (MQ=19)
                                                                                                                                                  gagtgggttttccccGTCGGTTGTGGGGCAGAGGCTCGCCCGGCTGGAAAGGGAAATCCTGGTTCACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGa                                                                    <  2:377321/215‑1 (MQ=11)
                                                                                                                                                  acggggggtgTTCCCGCCCGGTGTGGGTGAAGATGCCGAACCGGCGGAAAAGGAAACTCCGGGCGGACCGGGGGAGGGAGGAAAGCCGCCGCTGGCGGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGa                                                                    <  1:423211/222‑1 (MQ=11)
                                                                                                                                                               ccgccgGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAACACCAGATGGACCCGGTGTACACGCCGGCGCGAACAATCCACCTGTACCACTGCGCCCATCGCAGCCTGCCGCCGGCCCTTATCAGCAAGGAAGGGACAACAAAATgtgcg                                                      >  1:76992/1‑224 (MQ=255)
                                                                                                                                                                 gccgGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCCCTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATggcggt                                                     >  2:423211/1‑223 (MQ=37)
                                                                                                                                                                 cgtcgtggggggTGCAGAGGCAGGACGGGCTGGAAGTGGAATCCCGGGGTGTCCGGGTGAGGGAAGAAGGCAGCCGCTGTCGGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCAGCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTgcg                                                     <  1:951/218‑1 (MQ=11)
                                                                                                                                                                  ggcgggttggggtggatggccggACCGGTTGGAATGGGAAATCAGGGCTGGCCGGGGGTGTTGGGAACGCCGCCGCGGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCTTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTgcg                                                     <  1:619418/205‑1 (MQ=11)
                                                                                                                                                                      gTGCTGGTGCAGACGCTCGACCGGCTGGAAAGTGAACTCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGa                                                 <  2:10243/227‑1 (MQ=37)
                                                                                                                                                                       tGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGCAAGGCACACAAGAATGGTGCGCGGaag                                               >  2:271816/1‑227 (MQ=255)
                                                                                                                                                                          tggtccagggggtCGACGGGCGGGAAAGGGAAATCCTGGCGGACCGGGTAGGGGAGGAAAGCCGCCGCTGGCTGGCATCGGGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACg                                            <  2:265740/216‑1 (MQ=11)
                                                                                                                                                                                 gATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGCGCAGATGCAAAACCAACTGGACCCGGCGTACACGCCGCCGCGGAAAATCCCCCTGTACCACTCGCACCCTCGCGGCCTCCCGCCGGCCCTTACCACCAAGGAAGGGGCAACAGAATGGTggcccaaagaacaagaacg                                     >  1:1968/1‑210 (MQ=11)
                                                                                                                                                                                  ggtctctgcccgggtgAAAATGTAATCCCTGGCGGCCCGGGGGGGTAGGGAAAGCCGCCCGTGGCTGGCATCGGGCGGCCTGACCGTGGGGCCGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCCCTGCGACTATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATg                                     <  1:271816/213‑1 (MQ=11)
                                                                                                                                                                                             gcgggAAAGTGAAACCCGGCCGGACGGGGGGGGGGAGGAAAGCCGCCGCTGGCTGGCGTCGTGCGGCCTGACCGTGGAGAAGATGCAAAACCAGATGGACCCGTTGTACACGCCGGCGCGAAAAAGCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACctgc                          <  1:113341/224‑1 (MQ=11)
                                                                                                                                                                                                                tGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGCCACCAGAATGGTGCGCAGAATACGATGAAAGGGGCAACCTGCTGACAGAAGGAAACCCg         >  2:332660/1‑227 (MQ=255)
                                                                                                                                                                                                                  gCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAACCCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGGGCGGAATACGATGAATGGGGCAACCTGCGGAATGAAAAAAAACCGCaa      >  1:274762/1‑227 (MQ=255)
                                                                                                                                                                                                                  gCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGGGTACACGCCGCCGCGAAAAATCCACCTGTACCAATGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCCAGGAAGGGGAAACAGAATGGGGCCCAGAATACGATGAATGGGGCAACCTGCGGCATGCAGAAAACCCggcc      >  1:410013/1‑225 (MQ=11)
                                                                                                                                                                                                                     gACCGGGTGAGTGAGGAAAGCCGCCGCTGACTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCCCGAAAAATCCACCTGTACCACTGCGCCCATCGAGCCCCGCCGCTGGCCCTTATCAGCAAAGACGGGACACAAGAATGGCTCGCAGGATAACGTGACTGGGACAACCCGCCGCACGCAGAGAAACCGCAtaac   >  1:541342/1‑225 (MQ=11)
                                                                                                                                                                                                                      aCCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGACAAATCCACCTGTACCACTGCGCCCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAAGGACGAAAAaccaaaacaac  >  1:85378/1‑220 (MQ=11)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                           
AACGACAGAACCCGCATCCAGACGATTTATCAGCCGGGGAGCTTCACGCCACTCATCAGAGTTGAAACCGCCACCGGTGAGCTGGCGAAAACGCAGCGCCGCAGCCTGGCGGATGCGCTTCAGCAGTCCGGCGGCGAAGACGGTGGCAGTGTGGTGTTCCCGCCGGTGCTGGTGCAGATGCTCGACCGGCTGGAAAGTGAAATCCTGGCTGACCGGGTGAGTGAGGAAAGCCGCCGCTGGCTGGCATCGTGCGGCCTGACCGTGGAGCAGATGCAAAACCAGATGGACCCGGTGTACACGCCGGCGCGAAAAATCCACCTGTACCACTGCGACCATCGCGGCCTGCCGCTGGCCCTTATCAGCAAGGAAGGGACAACAGAATGGTGCGCAGAATACGATGAATGGGGCAACCTGCTGAATGAAGAGAACCCGCATCAGC  >  NC_000913/3765296‑3765734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: