| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 272,543 | T→G | 15.1% | intergenic (+354/‑37) | insN → / → eyeA | pseudogene, IS911 transposase A,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/novel sRNA, function unknown, CP4‑6 propahge |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 272,543 | 0 | T | G | 15.1% | 146.1 / 2.7 | 77 | intergenic (+354/‑37) | insN/eyeA | pseudogene, IS911 transposase A,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related/novel sRNA, function unknown, CP4‑6 propahge |
| Reads supporting (aligned to +/- strand): ref base T (27/34); new base G (11/3); total (40/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
CAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGA > NC_000913/272320‑272768 | cAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGCAGGTGtt > 2:378248/1‑227 (MQ=255) aCCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGCACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGAAACGTAAGCACTGCCGGTCGAAGGGGTTTTGACa > 2:241998/1‑228 (MQ=255) acAGGGTGTTTTACCCCGGTTACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCG‑AACACCg < 1:305947/227‑1 (MQ=255) aTGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTTCCGGTCGAAGGTGTTTTGACAT‑GTATGCG‑AACACCtggggcgca > 2:305947/1‑223 (MQ=255) gtGACCAGGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGACCCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTAt < 2:400831/226‑1 (MQ=255) tGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GGATGCGAAACACCGGGGCAGCAGCGTCTATc > 2:35346/1‑227 (MQ=255) aCAACGGGGATTGAGGCGAGATCCCTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACg < 2:156356/228‑1 (MQ=255) tggggcggaatccatAAGCCGAGATGAACCTAAAGGTTAAGTAATGAAAGGTTGAAGAACATGAACCCTTAAAGCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGGCTATCACGCTTGCGTTGCt < 1:108412/214‑1 (MQ=255) gaggtcagtaagccggtggtgaccTTAAGGTTAAGTCTTGAAAGGTTGAAGAACCTGAACCCGTTAATCCGCCGCTGTGGTTTGAAAACGGCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGAc < 1:186415/203‑1 (MQ=255) agaTCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCCGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCCGCGTCTATCACGCTTGTGTTGTTGACTTCt > 2:42439/1‑228 (MQ=255) agaTCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAG‑GTATGCGAAACACCGGGGCAGCAGCGTCTATACCGCTTGCGTTGCTGACTTCt > 2:592309/1‑228 (MQ=255) gatgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGCCGGGGTTTTGACAT‑GCATGCGAAACACCGGGTCACCAGCGTCTATCCCCCTTCCGTTCCTGACTTCTGCCACCTTTCGgcaa > 1:142322/1‑228 (MQ=255) gatgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTTGCGgcaa > 1:564698/1‑228 (MQ=255) ttgttCCTCAAGGTTAGGTCCGGAAAGGTTGAAGAACCTAAACCCGTTAATCCGCCTCTGGGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag < 1:394426/224‑1 (MQ=255) atgatCCTCATGTTTAAGTACTGAAAGGTTGAAAAACAAGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag < 2:457284/228‑1 (MQ=255) tttcccaaaGTTTAAGCACTGAAAGGTTGAAGACCATGAACCCGTTAATCCCCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca < 1:241998/221‑1 (MQ=255) tatcccaaagtttaatgtcTGAAAGGTTGAAAAACATGAACCGCTTAATCCGCCTCGGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca < 1:420172/210‑1 (MQ=255) gatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca < 2:348915/228‑1 (MQ=255) tttataaggaaaggggatcgAAAGGTTGAAGAAAATGAACCCGTTAACCCGCCTCTGGTGGTTGAAAACCTCCCCACGGCCTACGGTATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca < 1:600605/208‑1 (MQ=255) ccTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAgg > 1:193882/1‑228 (MQ=255) ccTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGCTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGCACGACGAGGTTTTGGCAT‑GTATGCGAAACACCGAGCCAGCAGCGCCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAgg > 2:619026/1‑228 (MQ=255) aGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCAACACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAACCACCGAGGCAGCAACGTCAGTCACGCTTGCGTTGCTGGACTCTGCACACTTCCGACAAGCACGGCAAAa‑ggagg > 2:616868/1‑223 (MQ=255) tGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAaaaaaggggggacgg > 1:259817/1‑217 (MQ=255) gAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGCGTTTTGACAT‑GTATGCGAAAAACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATTAA‑GAGGGCgcaggg > 2:340454/1‑223 (MQ=255) gAAAGGTTGAACCACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCGGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTGTTGACAT‑GTATGCGAAACACCGGGTCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTCCCAACGTGCGGCAAGCAAGGAAAAA‑AAGtcggacagg > 1:74350/1‑220 (MQ=255) aaGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCCATAACGCTTGCGTTGCTGCCTTCTCCCAACTTGCGGCAAGCAAGGATAAA‑GAGGGCGACgggac > 2:270221/1‑226 (MQ=255) ggtagaaaaccgtGAACCGGTTTATCCGCACGGTTGGGTTGAAAACCGCCACAGGGGCTACGTGAGCTTACAGGCCGTGAGGGAGGAACCGGGAGTGATACGGAAGCCCTGCCGGTCGAAGGTGTTTTGACAT‑TGATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGc < 1:253098/216‑1 (MQ=255) ttGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGGCTATCACCCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAAAAACGAG‑GGGACGGGCAGcc > 1:463478/1‑227 (MQ=255) ttGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGAACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGAATaaaaacctgcctctagtaca > 2:600605/1‑210 (MQ=255) aTGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACg > 2:401599/1‑157 (MQ=255) tGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGc < 1:401599/157‑1 (MQ=255) tGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGGTGCTGACTCCTGCCAACTTGCGGCAAGCAAGGATAAA‑AAGTGCGACGGGCAGCCCCCCCAGTATg > 2:269645/1‑228 (MQ=255) gaCCGGTTTATCCGGCTTGGTGGGTTGAAAAGGGCCCCCCGGCCTACGGGATCGGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGGTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGcc < 1:229230/227‑1 (MQ=255) caCCCGTTAACCCGCCTCTTTGGGTTGAAACCGTCCCCACGGCCTACGGGAGCTGACAGGCCGTGGAGGAGGAACGGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTTTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCGCAGTATGcc < 1:592309/227‑1 (MQ=255) aaCCTGTTAGACCGCCTGTGGGGGTTGAAAGCGAGTCCACGGCGTACGTGATCGGACAGGACTGGAAGGGGGAACTGGCAGTGAACCGTAAGCACTGCGGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGcc < 1:48373/228‑1 (MQ=255) ttAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGGATGCCtagttc > 2:156522/1‑223 (MQ=255) ttAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATCCCTGAGTc > 2:572643/1‑228 (MQ=255) tAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTTTGCCAACTTGCGGCAAGCAAGGATAAA‑AGGGGCGACGGGCACCCTCCCCCGCAAGCCTGAGAcc > 1:240629/1‑228 (MQ=255) gCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGa > 1:13460/1‑218 (MQ=255) ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGCCTTCTGCCAACTTTCGGCAAGCAAGGGTAAA‑GAGGGCGCCGGGCAGCCTCCTCCGTATGCCCGAGGCcagggaga > 1:478303/1‑227 (MQ=255) ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAg < 2:13460/218‑1 (MQ=255) ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGAAGCCTCCTCAGTATGCCTGGGTCcaggcagg > 2:48227/1‑228 (MQ=255) ccTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACCCTTGGGTTGCTGACTTCTGCCAACTTCCGGCAAGCAAGGGTGAA‑GAGGGCGACGGGCAGCCTCCCCCGTATGCCCGAGTcccagaagg > 1:332485/1‑221 (MQ=255) ctGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGAGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCCGACTTCTGCCAACTTGCGGCAAGCCAGGTTAAA‑GAGGGCACCAGGCGGCCTCCTCAGAATGCCCAGGGCCAGGCAGGGaa > 2:622274/1‑228 (MQ=255) gtgGGTTGAAAACGACCCCCGGGCCTACGTGATGTGAAAGGCCGTGCGGGAGGAAGTGGGTGTGATACGTAAGGAGTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAAc < 1:42439/228‑1 (MQ=255) tGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGa > 2:506308/1‑228 (MQ=255) gTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAg > 2:212523/1‑228 (MQ=255) caccacGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGAATGCCTGAGTCCCGGAAGGGAAACCGGGGAAGGTCACCg > 2:308157/1‑228 (MQ=255) ccgggCCTACGTGATCTGACAGGCCGTACAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACGT‑GTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg < 2:142322/225‑1 (MQ=255) cccGGCCTGGGTGCTCTTAGCGGCGGTGGGGGAGGAACTGGGAGTGAGACGGAAGCCCTGCCGGTCGAAGGTGTTTTGACAT‑GTATGAGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACg < 1:36773/226‑1 (MQ=255) ggCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGGTATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAt < 2:616746/228‑1 (MQ=255) ccTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGt > 2:505018/1‑228 (MQ=255) tACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAGGtt > 2:143910/1‑227 (MQ=255) tcGTGATCGGAAAGGCGGTGCAGGAGTAACTGGCAGGGAGACGTAAGCCCTGCCGGTCGAAGGGTTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACGTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGATGGATGTTaa < 1:626102/227‑1 (MQ=255) cggtaTCTGACAGGCCGGGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAg < 2:463478/224‑1 (MQ=255) tCTGACAGCCCGTGTAGTTGGAATTGGAAGTGTCTCGGACTCACTGGCTGTCGATTGTGTTTTGGGAT‑GTAGGAGAAACACCGGGGCAGCAGCGTCTAGCACGCTGGCGTTGCTGCGTTCTGCAAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGCCGGGCAGCCTCCTCAGGATGCATGAGTCCAGGCAGGTAATCCGGGGAAGGTCAGCGACGGATGTTAACGGGGc < 1:267585/228‑1 (MQ=255) ttGACAGGGCGTGCAGTGGGAACTGGCAGTGATACGGAAGCATTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGc < 1:48227/226‑1 (MQ=255) cTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCa > 2:65252/1‑228 (MQ=255) aCAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGATACCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAAGTAAAGGGGGCagg > 2:477272/1‑225 (MQ=255) cAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATgg > 2:66098/1‑227 (MQ=255) tGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGAGGTTAAGGGGGCATGGCTCCGATg > 2:527254/1‑228 (MQ=255) gaACTGGCAGTGATACGTAAGCACTTCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGAcg < 2:342025/223‑1 (MQ=255) gggATACGTAAGCCCTGCCGGTTGAAGGGGTTTTGACAT‑GTATGCGAAACCCCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCgg < 1:527254/225‑1 (MQ=255) gATTCGTAAGCCTTGCCGGTCGAAGGGGTTTTGACAT‑GTATGCGAAACAGCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 1:143910/228‑1 (MQ=255) gtACGTCAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACAGCGGGGCAGCAGCGTCTAGCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 2:326706/226‑1 (MQ=255) aTACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGc < 1:212523/227‑1 (MQ=255) tACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTCTGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCtt < 2:91028/228‑1 (MQ=255) gTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGGCAGCGACGGATGTTAAGGGGGAATGGCTCCGTTGACGCGCGGGCTGGCGGAGCTcccc > 2:569177/1‑227 (MQ=255) gctgtGCCGGGCGAAGGTTTTTTGACAT‑GTATGCGAAACACCGGGGGCGGCGCTCCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgtag < 1:65252/223‑1 (MQ=255) tGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGACGGTCAGCGACGGCTGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCgtaggcgtc > 2:305614/1‑223 (MQ=255) tGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCACAGACGGATGTTAAGGGGGCATGGATCCCATGACGCGCTGGCGGCCGGAGCTTCCgtagtatcc > 2:548129/1‑225 (MQ=255) ccGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGAAGTcc > 2:438478/1‑227 (MQ=255) tCGAAGGTGTTTTGACAT‑GTATGCGAAACCACGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGa < 2:259817/227‑1 (MQ=255) gttgtTTTGACGT‑GTATGCAGAACCCCGGGGCCGCAGCGGCTATCCCGCTTGCGTTGCTGCATTCTTCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGGCGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGATGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:269645/226‑1 (MQ=255) ggTTTTTTGACAT‑GTATGGGAAACCCCGGGGCAGCAGGGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:66098/228‑1 (MQ=255) ggTGTTTTGACATGGTATG‑GAACCCCCGGGGGAGAGGCGGTTATCACGCTTGGGTTGCTGGCTTCTGCCAACTGGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:569177/228‑1 (MQ=255) ggTGTTTTGACAT‑TTATGCGAAACACCGGGGCAGCAGCTTCTATCCCGGTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGGCGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 1:506308/228‑1 (MQ=255) ggTGTTTTGACAT‑GGATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGa < 2:240629/228‑1 (MQ=255) | CAGTGACCTGCGGCATGCCCGCAGGGTGATGTAACCCGCTGACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACAT‑GTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAA‑GAGTGCGACGGGCAGCCTCCTCAGTATGCCTGAGTCCAGGCAGGTAAACCGGGGAAGGTCAGCGACGGATGTTAAGGGGGCATGGCTCCGATGACGCGCTGGCTGGCGGAGCTTCCGTAGTAGTCCGCGATGGGGA > NC_000913/272320‑272768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |