Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,951,494 T→G 16.7% pseudogene (936/984 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,4940TG16.7% 176.1 / 3.3 79pseudogene (936/984 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base G (13/3);  total (45/34)
Fisher's exact test for biased strand distribution p-value = 2.74e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA  >  NC_000913/3951268‑3951719
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
tAGAAGCAGTTTACTCGTACTGTCGGGGGATGCTGGGGATGCACGGCCCCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGt                                                                                                                                                                                                                                    <  1:290824/227‑1 (MQ=255)
        gATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCCCGTTTTGATGACCGGGTGACAGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAACTGAACAAGCAGCGGCATGCACATGAGGCGATACAAGGGGATATaaac                                                                                                                                                                                                                           >  1:249010/1‑227 (MQ=255)
        gATTATCCGTACTATCTGGGCATGATGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGGGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGCGTTATCCAAATGGATATCGACCCGGCAAAAATGACCAAGCTGCGTCAGGCCCATTTGGAATTACAAGGGGACTTaaaa                                                                                                                                                                                                                           >  1:456767/1‑227 (MQ=255)
           tATCCGTACTATCTGGGGATGGTGGGGAGCACGGGACCCACAGCGGCAAGCTTCGCGGTGCAGGGGTGTGCCCTGCTGATCGCCGTGGGCGCACGGTTTGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGct                                                                                                                                                                                                                        <  1:340223/228‑1 (MQ=255)
               cGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGGGTTATCCATATGGATATGGACCCGGCAAAAATAAACAAGCTGCTTCAGGCACACGTGGCCTTACAAGGGGAATTAAAtccccgtt                                                                                                                                                                                                                    >  1:473057/1‑221 (MQ=255)
                  aCTATCTGGGGATGGTGGGGATGCACGCCACCAAAGCGGCAAACTGCGCGTGGCAGGAGCTTGACCTTCTGATCGCCGTGGGCGGCCGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc                                                                                                                                                                                                                 <  1:41688/228‑1 (MQ=255)
                   ttttCTGGGCCTGCTGGGGATGCACGGAACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGTCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc                                                                                                                                                                                                                 <  2:249010/224‑1 (MQ=255)
                   ccaTCTGGGAGTGGTGGGGATGCACGGACCCAAAGCGGCAAACGTCGCGGTGCAGGCGTGTGACCTGCTGATCGCCGGGGGCGCAAGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:589943/226‑1 (MQ=255)
                     aTCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:103358/226‑1 (MQ=255)
                     aTCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc                                                                                                                                                                                                                <  2:542721/226‑1 (MQ=255)
                              tGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATAGGGATATCGACCCGGCACGACTGAACAAGCTGCGTCAGGCCCATGTGGCCTTAACAGGGGATTTAAATGCCCTGTTACCAGAATTAcagc                                                                                                                                                                                                     >  1:481359/1‑228 (MQ=255)
                              tGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGAGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGCCAGGCACATGTGGCATTACAAGGGAATTTCAATGCTCTGTTACCAGCATTAcagc                                                                                                                                                                                                     >  2:125724/1‑228 (MQ=255)
                               gCTGGGGATGCAAGGCACCAAAGCGGCAATCTTGGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAcagca                                                                                                                                                                                                    <  2:366197/228‑1 (MQ=255)
                                cTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACTTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCACACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAACGAACAAGTTGGGTCAGGCACATGTGGACTTACACGTGGCTTTTAATGCCCTGTTCACACCCTTAcacatg                                                                                                                                                                                                   >  1:80564/1‑224 (MQ=255)
                                cTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACCCGCCAGTGTTATCCATATGGAAATCGACCCGGCAGAAATGACCAAGCGGCGTCAGGCACATGTGGCATTACAAGGGGATTTAACTGCCCTGTTCCCACCATTAcagcac                                                                                                                                                                                                   >  1:328162/1‑227 (MQ=255)
                                      aTGCAGGGCACCAAAGCGGAAAACTTCGGGGTGCAGGAGTGTAACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGtt                                                                                                                                                                                              <  2:523848/227‑1 (MQ=255)
                                        gcgcGGCCCAAAAGCGGCAAACTGCGGGGGGCAGGAGTGTGACCTGCTGATCGCCGTGGGCACAGGTTTGGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACGCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa                                                                                                                                                                                            <  1:266602/224‑1 (MQ=255)
                                         cACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt                                                                                                                                                                                          >  2:506084/1‑228 (MQ=255)
                                             gCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAAt                                                                                                                                                                                      >  2:100104/1‑228 (MQ=255)
                                                         gCAAACTTCGCGGTGCGGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGACATGAACAAGCTGCGTCAGGCACATGTGGCCTTACAAGGTGATTTAAATGCTCTGTTACCACCCATACAACAGCCGTTCAATCAAGGAcagtcacca                                                                                                                                                                           >  1:146484/1‑220 (MQ=255)
                                                         gCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGCCCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCCATGACTGgcagca                                                                                                                                                                           >  2:41996/1‑228 (MQ=255)
                                                            aaCTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACAGGTAGCaaa                                                                                                                                                                         >  2:447020/1‑226 (MQ=255)
                                                                        cAGGGGTGGGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCt                                                                                                                                                           <  2:566142/228‑1 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTTGGTCAGGACCCTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATTCATGGCAGCAACACTCCGCGCGACGGCGtggg                                                                                                                                                    >  1:78691/1‑225 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGCACAAGCTGCGTCAGGCACATGTGGCATTACAAGTGGATTTAAATCCTCTGATACCACAATTACAGGAGCCGTTCACTCAATGACTGACAGAAACACACCGCACAACTGCGcgagg                                                                                                                                                   >  1:142521/1‑226 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCCGAAAAACTGCCCGCAGCTGCGtgat                                                                                                                                                    >  2:466501/1‑227 (MQ=255)
                                                                                tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACCGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCAAATGTGGCATTACAAGGTGATTTAAATACTCTGTTACCAGCATTACCGCAGCCGTTAAATCAATAACTGTCAGCACCACTGAGcacacacgcgcgcgg                                                                                                                                                   >  1:443824/1‑217 (MQ=255)
                                                                                  aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGTCAGCAAAACTTCGCGCCGCTCCGtgatgac                                                                                                                                                 >  2:326751/1‑227 (MQ=255)
                                                                                  aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTGAAATCAATGACTGGCAGCAACCCTGCGCGCatcgcagtgctgac                                                                                                                                                 >  2:247920/1‑215 (MQ=255)
                                                                                   ccTGGTGATCGCCGGGGGCGCCGGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGaa                                                                                                                                                 <  2:512286/227‑1 (MQ=255)
                                                                                   ccTGCTGATCGCCGGGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGaa                                                                                                                                                 <  2:102748/227‑1 (MQ=255)
                                                                                            cGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTGTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACGGCGCGAACCTGCGGGTTGAACAATCCTgg                                                                                                                                        >  1:271976/1‑227 (MQ=255)
                                                                                                       cACTTTTTGGTGACCGGGTGCCCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt                                                                                                                            <  1:636375/228‑1 (MQ=255)
                                                                                                              tgatgaCCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCCCGCAGCTGCGGGATGAACATTCCTGGCGTTACAACCAAccccgg                                                                                                                      >  1:102851/1‑224 (MQ=255)
                                                                                                                    ccGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAGGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGACCAAAACCTGCCGGCACCCCCGTGATGAAAATTCCGTGCGTTACAGCCATCCCGGGGAcccca                                                                                                               >  1:340804/1‑224 (MQ=255)
                                                                                                                          taCCGGCAAACTGAACACCTTCGCGCACCACGCCAGTGTTATCCATATGGATAGCGACCCGGTAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg                                                                                                         <  2:645925/227‑1 (MQ=255)
                                                                                                                              ggCAAACTGAACACCTGCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCTTCAGGCAAATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgcg                                                                                                       <  2:481359/226‑1 (MQ=255)
                                                                                                                                 aaaCTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt                                                                                                  <  2:473057/228‑1 (MQ=255)
                                                                                                                                     ttaaCTCCTTCGCGCCACAACCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt                                                                                               <  2:271976/225‑1 (MQ=255)
                                                                                                                                            agtCGGGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa                                                                                        <  2:50731/225‑1 (MQ=255)
                                                                                                                                               cgcgCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaaa                                                                                                                                                                                           >  1:364298/1‑126 (MQ=255)
                                                                                                                                                gcgcCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt                                                                                                                                                                                          <  2:364298/126‑1 (MQ=255)
                                                                                                                                                 cgcCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTCAATCAATGACTGGCAGCAACACTCCGCGCAGCTGGGTGATGAAAATTCCTGGCGTTACGACCATCCCGGTGCCCACATCAACGCCCCGTTGTTTTTAAAAAAACtt                                                                                  >  1:494948/1‑227 (MQ=255)
                                                                                                                                                   ccACACGCCAGTGTGATCCATACGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCTGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAACAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTGCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt                                                                                 <  2:80564/227‑1 (MQ=255)
                                                                                                                                                    cacaCGCCTGTTTTATCCATATGGATATCGACCCGGCAAAAATGAACAAGCTGCGTCAGGCACATGGGGCATTACAAGGTGATTTAAATGCTCTGTTCCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               <  1:100104/228‑1 (MQ=255)
                                                                                                                                                      cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGCTGATTTAAATGATCTGTTACCAGCATTACAGCAGCCGTACAATCAATGACTGGCAGCAACACTGCGCGCAACGACGTGATGAACATTCCTGGCGTTACGCCCATCCCGGCGACGCTATCCACGACCCGTTGTTTGTCAaacaaccgcccgc                                                                             >  1:406353/1‑221 (MQ=255)
                                                                                                                                                         gCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGCCGTTACGACCATCCCGGTGCCGCCATCTACGCGCCGTTGTTGTTAAAACAACTTCCGGATCg                                                                          >  2:527380/1‑228 (MQ=255)
                                                                                                                                                           cgttgtTTTCCATTTGGATAGCGACCCGGGAAAAATGAACAAGCTGCTCCAGGCCCATGTGGCATTACAAGGTGATTTAAATGCCCTGTTACCAGCATTAACGCAGCCGTTAAATCCATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                                        <  1:125724/225‑1 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGCCGCAATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:182614/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:154881/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCACATGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:158113/1‑228 (MQ=255)
                                                                                                                                                                   tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGAGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                                >  2:41845/1‑228 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACAAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGt                                                                                                                                                       >  1:270885/1‑136 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCTGTGTTGTTAAAACAACTGTCGGATCGTAAACCCACGGATTg                                                           >  2:250578/1‑228 (MQ=255)
                                                                                                                                                                        atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGACCGTAAACCTCCGAATTg                                                           >  2:594771/1‑228 (MQ=255)
                                                                                                                                                                         tGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGtg                                                                                                                                                      <  2:270885/136‑1 (MQ=255)
                                                                                                                                                                          ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAACACTGTCGGCTCGTAAACCTGCGGATTGcg                                                         >  2:55692/1‑228 (MQ=255)
                                                                                                                                                                           gATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt                                                        >  2:558340/1‑228 (MQ=255)
                                                                                                                                                                              ctCGCCCCGGAAGAAATGACCAAGCTGCGTCAGGCACAGGGGGCATTACAAGGTGATTTAAATGCTCGGTTACCAGCATTACAGCGGCCGTTAAAGCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     <  1:655720/227‑1 (MQ=255)
                                                                                                                                                                              atCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTTGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     >  2:578386/1‑228 (MQ=255)
                                                                                                                                                                               tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTACATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCACCACTGCGAGCAGCTGCGTGATGAACATTTCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGCTGCTGTTAAACCAACTGTCGGTTCGTAACCCTGCGGATTGCGCCGTg                                                    >  2:160690/1‑228 (MQ=255)
                                                                                                                                                                               tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCCCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                                                    >  1:117969/1‑228 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                                                 >  2:116887/1‑228 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     >  2:180098/1‑224 (MQ=255)
                                                                                                                                                                                  aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt                                                     <  1:180098/224‑1 (MQ=255)
                                                                                                                                                                                    ccGTCAGAAATGAACAAGCTGCGTTAGGCACATGTGGCATTTCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                                               <  2:78691/228‑1 (MQ=255)
                                                                                                                                                                                     cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                                         >  2:430137/1‑201 (MQ=255)
                                                                                                                                                                                     cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTGGTTGTTAAAACAACTGACGGATCGTAAACCTGCGGAATGCGTCCTGACcaca                                              >  2:96692/1‑228 (MQ=255)
                                                                                                                                                                                      ggCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa                                                                        <  1:430137/201‑1 (MQ=255)
                                                                                                                                                                                               gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                    <  2:102851/228‑1 (MQ=255)
                                                                                                                                                                                               gAACAAGCGTGGGCAGGCACATGTGGACTTACAAGGTGATTTAAAGGCTCCTTTACCAGCATTACGGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGGGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                    <  1:506084/228‑1 (MQ=255)
                                                                                                                                                                                                  cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               >  2:584873/1‑182 (MQ=255)
                                                                                                                                                                                                  cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                               <  1:584873/182‑1 (MQ=255)
                                                                                                                                                                                                     gggggggcAGGCACAGGGGGCTATACAAGGGGAATGAAAGGCTCTGGTAACAGCATTAAAGCGGCCGTTAAAACAATGACTGGCAGCAAAACGGCGCGCAGCTGGGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc                               <  1:41996/220‑1 (MQ=255)
                                                                                                                                                                                                                 ccaTTTGGCATTACAAGGGTATTTAAATGCTCTGTTCCCAGCATTACAGCCGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTg                  <  1:635384/227‑1 (MQ=255)
                                                                                                                                                                                                                   atttgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCTTCGTGACCCCAGATGTGGGGCAGCACCAAATGTGGGCTgcg                >  2:635384/4‑228 (MQ=255)
                                                                                                                                                                                                                   aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCCGCACCAAAAGTGGGCTgc                 >  1:446563/1‑227 (MQ=255)
                                                                                                                                                                                                                    ttgggattttaaaGGTGATTTAAATCGTCTTTTCCCAGAATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACCGCGCGCAGCCGCGGGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcgc               <  1:527380/217‑1 (MQ=255)
                                                                                                                                                                                                                           ttACAAGTGGTTTGAAATGTTCTGGTGCCAGTATTACAGGAGGCGTTAAATCAATGTCTGGCAGCAACACTGCGCGCAGCTGCGTGATTAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca         <  1:55692/227‑1 (MQ=255)
                                                                                                                                                                                                                                 ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGAAGTGGGGCAGCACCAAATTTGGGCTGGGCAGCACACCCCCca  >  1:321911/1‑228 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCACCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA  >  NC_000913/3951268‑3951719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: