Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,951,494 | T→G | 16.7% | pseudogene (936/984 nt) | ilvG → | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,951,494 | 0 | T | G | 16.7% | 176.1 / 3.3 | 79 | pseudogene (936/984 nt) | ilvG | pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive, acetolactate synthase II, large subunit, cryptic, interrupted |
Reads supporting (aligned to +/- strand): ref base T (31/31); new base G (13/3); total (45/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.74e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA > NC_000913/3951268‑3951719 | tAGAAGCAGTTTACTCGTACTGTCGGGGGATGCTGGGGATGCACGGCC‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGt < 1:290824/227‑1 (MQ=255) gATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCCCGTTTTGATGACCGGGTGACAGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAACTGAACAAGCAGCGGCATGCACATGAGGCGATACAAGGGGATATaaac > 1:249010/1‑227 (MQ=255) gATTATCCGTACTATCTGGGCATGATGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGGGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGCGTTATCCAAATGGATATCGACCCGGCAAAAATGACCAAGCTGCGTCAGGCCCATTTGGAATTACAAGGGGACTTaaaa > 1:456767/1‑227 (MQ=255) tATCCGTACTATCTGGGGATGGTGGGGA‑GCACGGGACCCACAGCGGCAAGCTTCGCGGTGCAGGGGTGTGCCCTGCTGATCGCCGTGGGCGCACGGTTTGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGct < 1:340223/228‑1 (MQ=255) cGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGGGTTATCCATATGGATATGGACCCGGCAAAAATAAACAAGCTGCTTCAGGCACACGTGGCCTTACAAGGGGAATTAAAtccccgtt > 1:473057/1‑221 (MQ=255) aCTATCTGGGGATGGTGGGGATGCACGCCA‑CCAAAGCGGCAAACTGCGCGTGGCAGGAGCTTGACCTTCTGATCGCCGTGGGCGGCCGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc < 1:41688/228‑1 (MQ=255) ttttCTGGGCCTGCTGGGGATGCACGGAA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGTCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAc < 2:249010/224‑1 (MQ=255) ccaTCTGGGAGTGGTGGGGATGCACGGAC‑CCAAAGCGGCAAACGTCGCGGTGCAGGCGTGTGACCTGCTGATCGCCGGGGGCGCAAGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 2:589943/226‑1 (MQ=255) aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 2:103358/226‑1 (MQ=255) aTCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTAcc < 2:542721/226‑1 (MQ=255) tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATAGGGATATCGACCCGGCACGACTGAACAAGCTGCGTCAGGCCCATGTGGCCTTAACAGGGGATTTAAATGCCCTGTTACCAGAATTAcagc > 1:481359/1‑228 (MQ=255) tGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGAGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGCCAGGCACATGTGGCATTACAAGGGAATTTCAATGCTCTGTTACCAGCATTAcagc > 2:125724/1‑228 (MQ=255) gCTGGGGATGCAAGGCA‑CCAAAGCGGCAATCTTGGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAcagca < 2:366197/228‑1 (MQ=255) cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACTTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCACACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAACGAACAAGTTGGGTCAGGCACATGTGGACTTACACGTGGCTTTTAATGCCCTGTTCACACCCTTAcacatg > 1:80564/1‑224 (MQ=255) cTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACCCGCCAGTGTTATCCATATGGAAATCGACCCGGCAGAAATGACCAAGCGGCGTCAGGCACATGTGGCATTACAAGGGGATTTAACTGCCCTGTTCCCACCATTAcagcac > 1:328162/1‑227 (MQ=255) aTGCAGGGCA‑CCAAAGCGGAAAACTTCGGGGTGCAGGAGTGTAACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGtt < 2:523848/227‑1 (MQ=255) gcgcGGCC‑CAAAAGCGGCAAACTGCGGGGGGCAGGAGTGTGACCTGCTGATCGCCGTGGGCACAGGTTTGGAGGACCGGGTGACCGGCAAACTGAACCCCTTCGCGCCACGCGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaa < 1:266602/224‑1 (MQ=255) cACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt > 2:506084/1‑228 (MQ=255) gCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAAt > 2:100104/1‑228 (MQ=255) gCAAACTTCGCGGTGCGGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCTCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGACATGAACAAGCTGCGTCAGGCACATGTGGCCTTACAAGGTGATTTAAATGCTCTGTTACCACCCATACAACAGCCGTTCAATCAAGGAcagtcacca > 1:146484/1‑220 (MQ=255) gCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGCCCCGGCAGAAATGACCAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCCATGACTGgcagca > 2:41996/1‑228 (MQ=255) aaCTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACAGGTAGCaaa > 2:447020/1‑226 (MQ=255) cAGGGGTGGGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCt < 2:566142/228‑1 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCCCACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTTGGTCAGGACCCTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATTCATGGCAGCAACA‑CTCCGCGCGACGGCGtggg > 1:78691/1‑225 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGCACAAGCTGCGTCAGGCACATGTGGCATTACAAGTGGATTTAAATCCTCTGATACCACAATTACAGGAGCCGTTCACTCAATGACTGACAGAAACA‑CACCGCACAACTGCGcgagg > 1:142521/1‑226 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCCGAAAAA‑CTGCCCGCAGCTGCGtgat > 2:466501/1‑227 (MQ=255) tgACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACCGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCAAATGTGGCATTACAAGGTGATTTAAATACTCTGTTACCAGCATTACCGCAGCCGTTAAATCAATAACTGTCAGCACCA‑CTGAGcacacacgcgcgcgg > 1:443824/1‑217 (MQ=255) aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGTCAGCAAAA‑CTTCGCGCCGCTCCGtgatgac > 2:326751/1‑227 (MQ=255) aCCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTGAAATCAATGACTGGCAGCAACC‑CTGCGCGCatcgcagtgctgac > 2:247920/1‑215 (MQ=255) ccTGGTGATCGCCGGGGGCGCCGGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa < 2:512286/227‑1 (MQ=255) ccTGCTGATCGCCGGGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGaa < 2:102748/227‑1 (MQ=255) cGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTGTCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CGGCGCGAACCTGCGGGTTGAACAATCCTgg > 1:271976/1‑227 (MQ=255) cACTTTTTGGTGACCGGGTGCCCGGCAAACTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAt < 1:636375/228‑1 (MQ=255) tgatgaCCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGGGATGAACATTCCTGGCGTTACAACCAAccccgg > 1:102851/1‑224 (MQ=255) ccGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAGGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGG‑ACCAAAACCTGCCGGCACCCCCGTGATGAAAATTCCGTGCGTTACAGCCATCCCGGGGAcccca > 1:340804/1‑224 (MQ=255) taCCGGCAAACTGAACACCTTCGCGCACCACGCCAGTGTTATCCATATGGATAGCGACCCGGTAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg < 2:645925/227‑1 (MQ=255) ggCAAACTGAACACCTGCGCACCACACGCCAGGGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCTTCAGGCAAATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgcg < 2:481359/226‑1 (MQ=255) aaaCTGAACCCCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgtt < 2:473057/228‑1 (MQ=255) ttaaCTCCTTCGCGCCACAACCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgtt < 2:271976/225‑1 (MQ=255) agtCGGGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGAATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaaa < 2:50731/225‑1 (MQ=255) cgcgCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTaaa > 1:364298/1‑126 (MQ=255) gcgcCACACGCCAATGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAt < 2:364298/126‑1 (MQ=255) cgcCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTCAATCAATGACTGGCAGCAACA‑CTCCGCGCAGCTGGGTGATGAAAATTCCTGGCGTTACGACCATCCCGGTGCCCACATCAACGCCCCGTTGTTTTTAAAAAAACtt > 1:494948/1‑227 (MQ=255) ccACACGCCAGTGTGATCCATACGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCTGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAAACAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTGCGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGt < 2:80564/227‑1 (MQ=255) cacaCGCCTGTTTTATCCATATGGATATCGACCCGGCAAAAATGAACAAGCTGCGTCAGGCACATGGGGCATTACAAGGTGATTTAAATGCTCTGTTCCCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg < 1:100104/228‑1 (MQ=255) cacGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGCTGATTTAAATGATCTGTTACCAGCATTACAGCAGCCGTACAATCAATGACTGGCAGCAACA‑CTGCGCGCAACGACGTGATGAACATTCCTGGCGTTACGCCCATCCCGGCGACGCTATCCACGACCCGTTGTTTGTCAaacaaccgcccgc > 1:406353/1‑221 (MQ=255) gCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGCCGTTACGACCATCCCGGTGCCGCCATCTACGCGCCGTTGTTGTTAAAACAACTTCCGGATCg > 2:527380/1‑228 (MQ=255) cgttgtTTTCCATTTGGATAGCGACCCGGGAAAAATGAACAAGCTGCTCCAGGCCCATGTGGCATTACAAGGTGATTTAAATGCCCTGTTACCAGCATTAACGCAGCCGTTAAATCCATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa < 1:125724/225‑1 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGCCGCAATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 2:182614/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 2:154881/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCACATGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 2:158113/1‑228 (MQ=255) tCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGAGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg > 2:41845/1‑228 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACAAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGt > 1:270885/1‑136 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTTCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCTGTGTTGTTAAAACAACTGTCGGATCGTAAACCCACGGATTg > 2:250578/1‑228 (MQ=255) atGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGGGATTTAAATGCTCTGTTACCAGCATTAAAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGACCGTAAACCTCCGAATTg > 2:594771/1‑228 (MQ=255) tGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGtg < 2:270885/136‑1 (MQ=255) ggATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAAACACTGTCGGCTCGTAAACCTGCGGATTGcg > 2:55692/1‑228 (MQ=255) gATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGCGACGCTATCTCCGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt > 2:558340/1‑228 (MQ=255) ctCGCCCCGGAAGAAATGACCAAGCTGCGTCAGGCACAGGGGGCATTACAAGGTGATTTAAATGCTCGGTTACCAGCATTACAGCGGCCGTTAAAGCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt < 1:655720/227‑1 (MQ=255) atCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGTCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTTGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt > 2:578386/1‑228 (MQ=255) tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTACATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCACCA‑CTGCGAGCAGCTGCGTGATGAACATTTCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGCTGCTGTTAAACCAACTGTCGGTTCGTAACCCTGCGGATTGCGCCGTg > 2:160690/1‑228 (MQ=255) tCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCCCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg > 1:117969/1‑228 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACACCTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc > 2:116887/1‑228 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt > 2:180098/1‑224 (MQ=255) aCCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcgt < 1:180098/224‑1 (MQ=255) ccGTCAGAAATGAACAAGCTGCGTTAGGCACATGTGGCATTTCAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac < 2:78691/228‑1 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt > 2:430137/1‑201 (MQ=255) cGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCCATCTACGCGCCGTGGTTGTTAAAACAACTGACGGATCGTAAACCTGCGGAATGCGTCCTGACcaca > 2:96692/1‑228 (MQ=255) ggCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTTATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTa < 1:430137/201‑1 (MQ=255) gAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc < 2:102851/228‑1 (MQ=255) gAACAAGCGTGGGCAGGCACATGTGGACTTACAAGGTGATTTAAAGGCTCCTTTACCAGCATTACGGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGGGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc < 1:506084/228‑1 (MQ=255) cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg > 2:584873/1‑182 (MQ=255) cAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCg < 1:584873/182‑1 (MQ=255) gggggggcAGGCACAGGGGGCTATACAAGGGGAATGAAAGGCTCTGGTAACAGCATTAAAGCGGCCGTTAAAACAATGACTGGCAGCAAAA‑CGGCGCGCAGCTGGGTGATGAACATTCCTGGCGTTACGACCAGCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc < 1:41996/220‑1 (MQ=255) ccaTTTGGCATTACAAGGGTATTTAAATGCTCTGTTCCCAGCATTACAGCCGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTg < 1:635384/227‑1 (MQ=255) atttgGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCTTCGTGACCCCAGATGTGGGGCAGCACCAAATGTGGGCTgcg > 2:635384/4‑228 (MQ=255) aTGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGGTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGATGTGGGGCCGCACCAAAAGTGGGCTgc > 1:446563/1‑227 (MQ=255) ttgggattttaaaGGTGATTTAAATCGTCTTTTCCCAGAATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CCGCGCGCAGCCGCGGGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTgcgc < 1:527380/217‑1 (MQ=255) ttACAAGTGGTTTGAAATGTTCTGGTGCCAGTATTACAGGAGGCGTTAAATCAATGTCTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATTAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca < 1:55692/227‑1 (MQ=255) ggTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGCCGTGACCACAGAAGTGGGGCAGCACCAAATTTGGGCTGGGCAGCACACCCCCca > 1:321911/1‑228 (MQ=255) | TAGAAGCAGATTATCCGTACTATCTGGGCATGCTGGGGATGCACGGCA‑CCAAAGCGGCAAACTTCGCGGTGCAGGAGTGTGACCTGCTGATCGCCGTGGGCGCACGTTTTGATGACCGGGTGACCGGCAAACTGAACACCTTCGCGCCACACGCCAGTGTTATCCATATGGATATCGACCCGGCAGAAATGAACAAGCTGCGTCAGGCACATGTGGCATTACAAGGTGATTTAAATGCTCTGTTACCAGCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACA‑CTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCA > NC_000913/3951268‑3951719 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |