Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,511,532 | T→C | 17.3% | E295G (GAG→GGG) | fecD ← | ferric citrate ABC transporter permease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,511,532 | 0 | T | C | 17.3% | 108.4 / 2.9 | 57 | E295G (GAG→GGG) | fecD | ferric citrate ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (8/34); new base C (3/8); total (13/44) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGAATCGGGTATGGGG > NC_000913/4511305‑4511752 | aaaGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCt < 2:564850/228‑1 (MQ=255) aaatCAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTgg‑ggg < 2:157459/228‑1 (MQ=255) aaacaatttttaccggctCGGTTTCCCGCAACCGTTAGGGCCTGAT‑AGGGCGGTTATCG‑TCCCCGTTGGCAGTGAGAGTGAAACGGCGTTAAGTTCCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTgg‑ggg < 1:342122/211‑1 (MQ=255) aaaaCAGTTTAACGGGGTCGAGTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGGTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTgg‑ggg < 1:156802/228‑1 (MQ=255) aaaaCAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTgg‑ggg < 1:400642/228‑1 (MQ=255) tAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGaataa < 2:234689/227‑1 (MQ=255) cAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAACTACCTTGTCTGTCCCGTAACTGACCGTGAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACAAGACAAACCACGGCGCACCGATAATGTCGGTCCGCCAGACA‑ACCAGGAGCGcccacggggggaggaaaactaa > 1:543051/1‑208 (MQ=255) cGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGGCCGCCA‑ACCGGGAGCCCCAGTgg‑gggggtgataattttcg > 2:477796/1‑213 (MQ=255) gTCCATTTCCCGCAACCGTTAGGAAC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGGCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGcc < 2:6934/228‑1 (MQ=255) gATTTCCCGCAACCTTTAGGACG‑CGTCCGGG‑GGGGGTCTGTCACCGTTGGCAGTGAGAGAGAAACGTCGTGAAGTACCTTGGCTGTCCCGTAACTGACCGGCAGATTTTCAGTTCGTAAAGTCATTTAACGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATACTGCTCGc < 1:250440/224‑1 (MQ=255) gATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCcagc < 1:67475/228‑1 (MQ=255) gCAACCGGTGGGGCC‑GATTAGGGCGGGTATTT‑TCCCCGTTGGCAGTGAGAGTTAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCgg < 1:391017/227‑1 (MQ=255) aGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCaa < 2:412858/228‑1 (MQ=255) aGGACC‑GATCAGGGCGGTGATCT‑TCCCCCTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTAGCGCATTCTCACAAGCCACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCaa < 2:81010/228‑1 (MQ=255) aGGACC‑CATCAGGGTGGTGAATT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATCCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCaa < 2:418920/228‑1 (MQ=255) ccagtcgcgggtggtctt‑tCCCAGGTGGCAGTGAGAGTGAAACGTAGTTAAGTTCCTTGTCTGGCCCGTAACTGACCGGCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCCAACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCCCGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑Ca < 1:573476/211‑1 (MQ=255) aTCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCCGCACGCCA‑ACCCGGAGCTCCAGTGG‑GGGGTGAATAATTCTCGCCACCAGAACGGC‑AACCCCCAAACG‑CAAc > 2:400261/1‑228 (MQ=255) aGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTCTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCa < 2:57067/228‑1 (MQ=255) gTGCTCC‑TCCCCGGTGGCAGGTAGAAGCAAACCTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTc < 2:543051/228‑1 (MQ=255) gTGATCT‑TCCCCGTTGGCAGTGAGATTGAAACGTCTTTAAGTACCTTGGCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTAGCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTc < 2:228917/228‑1 (MQ=255) tGATCT‑TCCCCGTTGGCCGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTACCTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACAACGGCGCACCGATAATGGCGGGCACCACGCCACACCGTACGC‑CCAGGTG‑GGGGTGCAAAATTTTCCCCAGCCGATACGC‑AACCACCAAccaccactccccactc > 1:528331/1‑212 (MQ=255) tGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑AACGGGAGCTCCCGTGG‑GGGGTGAATAATTCTCGCCCGCAGATCGGC‑AACCACCAACAG‑CAAGGCAACAGTCa > 2:356466/1‑228 (MQ=255) tGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑AACGGGAGCTCCAGGGG‑GGGAGGAATAATTCTTGCCACCAGATCGGC‑AACCACCAACCA‑GAACGCAcactgca > 2:117607/1‑222 (MQ=255) tCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGGTAAGTCCCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCCTTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAggg < 2:389247/228‑1 (MQ=255) ttctCCCCGTTGTCTGTGAGACGGAAACTTCGTTAAGTTCCTTTTCTTTCCCGTAACCGCCCTTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTTCCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAggg < 1:499958/225‑1 (MQ=255) gtcccccGTGTGCAGTGAGAGTGAAACGTCGTTAAGTTCCTTGGCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTAACGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGc < 2:232255/225‑1 (MQ=255) ttGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCC‑ACCGGGAGCTCCCGTGG‑GGGATGAATAATTCTCGCCAGCAGACCGGC‑AACCACCAACAG‑CAAAGCACCTGGCAGGGCCGaaaacg > 2:501386/1‑225 (MQ=255) ggCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTAACCAACCCC‑ACCGGGAGCCCCAGTGG‑GGGGTGAATAATTCTCGCCCGCAGAGCGGCGAACCA‑CAACAG‑CCAACCACCAGTCAgaggggacaaaagc > 1:590157/1‑215 (MQ=255) tGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCC‑ACCCGGAGCTCCAGTAG‑GGGATGGATAATTCTCGCCCGCCGATCCGC‑AAACACCCACAG‑CAACACACCTGTCAGGGCCGAAACAGGacgcag > 2:542208/1‑228 (MQ=255) gagaGTTAACCGCCGTTAAGTTCCTTTTCTTTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCGCCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGt < 1:626546/228‑1 (MQ=255) tttAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTCCAGTTCGAAAAGTCATCTATCGCATTCTCACAAGCAACCAGACAAGCCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTc < 2:590157/226‑1 (MQ=255) ttAAGTTCCTTGTCTGTCCCGTAACTTACCGTCAGATTTTCAGTTCGTAAAGTCATTTAGCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTcc < 2:405002/227‑1 (MQ=255) gttctttgttttgcccGTAACTGTCCGTCAGATTTTCAGGTCGTAAAGTCATTTATCGCATTCTCACAAGAAACAAGACAAACCCCGGCGCACCGATAAGGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGAGGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGAAGTCTGCGGTGACGTCCACCgg < 1:130701/215‑1 (MQ=255) gtgccTTGGCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTAGCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCgg < 2:528331/225‑1 (MQ=255) atcccTTTTCTTGCCCGTAACGGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCgg < 1:477796/225‑1 (MQ=255) ctcttttttgtcCCGTAAATGACCGGTAGATTTTCAGTGCGTAAAGTCAGTTACCGCATTCGCCCAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTg < 1:72502/220‑1 (MQ=255) tgtctgtcCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCAGGAGCCCCCGTGG‑GGGGTGAAAAATTCTCGCCCGCAGATCGGC‑AACCACCAACAG‑CAACGCACCCGTTAAGGCCCAAAAAGGCAGCAGACCGCGGGGaacacacccgcggagag > 1:315909/1‑212 (MQ=255) gtcCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCCGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGGCCGGGCCGAAACAGGCAACAGTCCGCGGTGACGTCCACCCGGGATGCTACg > 2:85269/1‑227 (MQ=255) tcCCTTTACTGCCCGTCAGATTTTCAGTTCGTAAAGTTATTTATCGCATTCTCACAAGCAACCAGACAACCCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCATATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGca < 1:501386/228‑1 (MQ=255) ggagatgtgtaaaagggacagaTCGCATTCCCAAAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACgaga < 2:141504/206‑1 (MQ=255) cGTAAATTCTATTATCGCATTCCCACAAGCAACCCGACAAACCCCGGCGCAACGATAATGGCGGGCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAg < 1:184375/228‑1 (MQ=255) tccccaagacaacagaaCAAACCACGGCGCCCCGTAAAGGGCGGTCAGCACGCCA‑ACGGGTGGCGCAAGTGG‑GGGATGAATAAGTCGCGCCAGCAGATCTGC‑AACCACCAACAG‑CAACCCACCTGGCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACTTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAggcggc < 1:642114/212‑1 (MQ=255) caacaaGACAAACCCCGGCGCCCCGTAAATGGCGGCCAGCCCGCCA‑ACCGGGAGCCCCAGGGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCCCCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGAGGCAGTCTGCGGGGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGt < 1:225225/228‑1 (MQ=255) ccAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCCGGAGCTCCAGTGG‑GGGATGAAAAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCCGTCAGGGCCGAAACAGGCAGCAGGCTGCGGGGACGTCCACCGGTGATGGCGCGCATCAAATGCGGCACCACGAGACCCATAAAGCAAATCGGGCGCCAGGCGGCCACCCCGGtgaa > 1:151737/1‑225 (MQ=255) cggtgcgcccGATAAGGGTGGACAGACCGCCA‑CCGGGAACCTCCAGGGG‑GGCATGAATAATGCTCGCCAGCAGGGCGGC‑AACCACCAACAG‑CACCGCACCTGTCAGGGCAGAAACGGGCAGCAGTCTGCGGTGACGTCCACCGGTGAGGCTACGCATCATATGCGGCCCCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGa < 1:229797/220‑1 (MQ=255) cgggggcACCGATAATGGCCGACAGCGCGCAA‑ACCGGGAGACCCAGGGG‑GGGAGGAATATTTCCGGGCAGCAGGTCGTG‑AAGCCCCAACAG‑CAACGCGCCTGTCAGGGCCGAAACAGGGAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATAGGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGa < 1:214328/223‑1 (MQ=255) ccGGGGCACCGAAAATGGGGGTGAGAGCTCCA‑CACGGAAGCGCCAGGGT‑GGGAGGAATAATTCCGGCCAGAAGAACGGC‑AACCACGAACAG‑CAACGCACCGGTCAGGGCCGAAACAGGCGGCAGTCTGCGGTGACGCCCACCGGTGATGCTACGCATCATAGGCGGCACCACGAGACCAATAAGGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGa < 1:33533/227‑1 (MQ=255) ccGGCGCACCGATTAGGGCGGTCCACACCCCA‑ACCGGGAGCTCCAGGGG‑GGGATGAAATATTCCCGCCAGCAAATCGGC‑AACCCCCAACCG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGGAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGa < 1:400261/227‑1 (MQ=255) cccccGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCCCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCt < 2:147514/225‑1 (MQ=255) aCCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAAAAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGGCTGCGGGGCAGCCCACCCGGGGTGCTGCGCATCAAATGCGGCCCCACCGGCCCAATAAACACAATCGGGGCCCCGGCGGCCCCCCCggcgggaggacagcagcaacaaaa > 1:193776/1‑206 (MQ=255) tAATGGCGGTCAGCCGGCCA‑CCCGGGAGCCCCAGGGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACa < 2:326815/228‑1 (MQ=255) gcgtccaccACTCCC‑ACCGGGAGCCCCCGTGG‑GGGATGAATAATTCCCCCCAGCATATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGcc < 1:356466/220‑1 (MQ=255) cGGTCAGCACGCCA‑ACCGGGATCCCCAGGGG‑GGAATGAAAAATTCTCGCCGGCAGCTCGGC‑AACCACCAACAG‑CACCGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGcc < 2:560944/227‑1 (MQ=255) ggTCAGCACCCCA‑CCGGGGGCCTCCCGGGG‑GGGATGAATAATGTCCGCCCGCAGACCGGA‑AACCCACAACAC‑CAAGCCACGTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCCCCACGAGACCAATAAAGCGAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCa < 1:372374/228‑1 (MQ=255) gcgccGCCA‑ACCGGGAGCTCCAGGGG‑GGGTTGATAAATTCCCGCCGGCAGATCGGC‑AACGACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCGGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGaa < 1:244773/223‑1 (MQ=255) gcccggcccagcGGGACCCCCAGTGGCGAGAT‑AATAAGTATCGCCAGCACATGGGC‑AACCACCGACCGCCACCGCACC‑GTGAGGGCAGAAACAGGCAGCCGTCTGCGGTGACGTCCACGGGTGATGCTACGCAGCATATGCGGCACCCGGAGACCAATAAAGCTAATCGGGCCGCAGTCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGAAt < 1:450243/217‑1 (MQ=255) ccGGAAGCTCCAGGGG‑GGGAGGAATAAGTCTCGCCAGCAGATCGGC‑AACCACCACCAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCCGCGGGGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGAATCGGGTATgg < 2:652132/227‑1 (MQ=255) gAGCCCCAGTGG‑GGGATGAATAATTCTCGCCAGCCGATCGGC‑AACCACCAACAG‑CCACGCCCCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGAATCGGGTATgggg < 2:151737/225‑1 (MQ=255) | AAAGCCGCGAAAAACAGTTTAACAGCGTCGATTTCCCGCAACCGTTAGGACC‑GATCAGGGCGGTGATCT‑TCCCCGTTGGCAGTGAGAGTGAAACGTCGTTAAGTACCTTGTCTGTCCCGTAACTGACCGTCAGATTTTCAGTTCGTAAAGTCATTTATCGCATTCTCACAAGCAACCAGACAAACCACGGCGCACCGATAATGGCGGTCAGCACGCCA‑ACCGGGAGCTCCAGTGG‑GGGATGAATAATTCTCGCCAGCAGATCGGC‑AACCACCAACAG‑CAACGCACCTGTCAGGGCCGAAACAGGCAGCAGTCTGCGGTGACGTCCACCGGTGATGCTACGCATCATATGCGGCACCACGAGACCAATAAAGCTAATCGGGCCGCAGGCGGCCACGCCGGTAGATGTCATGGCGACAGCTAGTAACAAAGCCCAGAATCGGGTATGGGG > NC_000913/4511305‑4511752 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |