| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 489,597 | T→G | 14.2% | V1021G (GTG→GGG) | mscK → | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 489,597 | 0 | T | G | 14.2% | 163.2 / 2.4 | 80 | V1021G (GTG→GGG) | mscK | mechanosensitive channel protein, intermediate conductance, K+ regulated |
| Reads supporting (aligned to +/- strand): ref base T (28/38); new base G (11/2); total (40/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCT > NC_000913/489376‑489807 | tATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCAAGCGTCTGATCAACTGGTCGTTGTCTGACACTACTACGCGTCTGGGGATCCGTCTCGCGTTGCCCTAGTGCCCCAATCTGGAAAACGTGGGCAAAATGTTCCt > 1:250494/1‑228 (MQ=255) cGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGCGATCCGTCTCAGCGTGGCCTATGGCTCCGCTCTGGAAAAAGTGCGTCAAGTGTAACtcacgg > 2:258152/1‑222 (MQ=255) tACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTCCTACACGTCTGGTGATCCGTCTCGGCGGGGCCTATGGCCCCGATCTGGAAAAAGGGCGTAAGGGGTTACT‑GAAggcgg > 2:80146/1‑227 (MQ=255) aCGGTAACCATTGGTAGCTTCTCGGGGAAGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGCCGTTGACTGACATTCATACGCGTCGGGCGACCCGTCCCGGCTTGGCCATGGGCCCCGACCTGGACACAGTGGGTAAAGGGttcattaagtcggga > 1:325797/1‑215 (MQ=255) aaCCATTGGTATCTTCTCGGGGACGGGAAGGAAGATCCGTATTCGTGCGACAACGATTAGCGATTTCGATCGAAAAGAAGTGATCCTCCCGAACAAAGCGTTGGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGa < 2:237342/228‑1 (MQ=255) actaTTGGTAGCTTCGCGGGGACGTGAAGTAAGATCCGTATCCGGGCGACAACTATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAg < 1:356639/225‑1 (MQ=255) aacATTGGTAGCTTCGCGGGGACGGTAAGTAAAATCCGTATTCGGGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAg < 2:579039/226‑1 (MQ=255) ccgtgggtgttttgtgggaACCGTAAGTAAGATCCGTTATGCTGCTGCGACGGTTTCCGATTTCGATTCCAAAGGAGTGATCATCCCGAACAAAGCGTTTGGTACCGCGCGTCTGATCCACGGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCa < 1:397222/213‑1 (MQ=255) tgggagtctcgcGGGGAAGGTAAGTAAGAGCCGGATGCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCAccc < 2:131336/217‑1 (MQ=255) ggTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCa > 2:251821/1‑228 (MQ=255) gTAGCTTCTCGGGGACGGTAAGTAAGGTCCGTAGGCGGGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATGCCGAACAAAGCGTTTGATACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCaa < 2:251894/228‑1 (MQ=255) tctcGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGACCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTAGGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCACGGGG‑Ga > 2:252363/1‑228 (MQ=255) gtaagtaaGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGCTGCCCATGGCCCCGATCTGGAAAACGTGCGGAAAGGATTCCT‑GAAGGCGGCGACCG‑GCAccccccgggggatgcaccaccc > 1:251574/1‑208 (MQ=255) caTCCGTTTTCGTGCGAAAACGATTCCCGATTTCGATCGCAAAGAAGTGATCACCCCGAACAAAGCGTTTGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCgg < 1:251821/226‑1 (MQ=255) aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGTTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GTAGGCGGCGCCTGAGCACACAAGGGT‑GATGCACGAACCAATG‑CCGGaa > 2:159617/1‑228 (MQ=255) aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGaa > 2:467551/1‑228 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGTAAAAGGTGCGTAAAGTGGTACT‑GATGGGGGCGACTGAGCACCCCAGGGT‑GTTGCATCAACCacaacgagcggt > 1:106202/1‑217 (MQ=255) ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGAAAAGTGTTACT‑GAAGGCGGCGCCTGAGCACCCAAGGGT‑GATGCACGACCCACTG‑CCGGCAg > 2:388029/1‑227 (MQ=255) tATTCGTGCGACAACGATTCCCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑g < 1:480080/175‑1 (MQ=255) tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATAACCTGGCCGTTGACTGACACTACTACGCGTCTGGTGGTCCGTCTCGGCGTGGCCTATGGCTCCGGTCTGGGAAAAGGGCGTAAGGTGTTAGT‑CAAGGCGTCGACTGAGGAGCCACAGGC‑GATGCAAGAACCAATT‑CCGGaatgctc > 1:612164/1‑223 (MQ=255) ttCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGCCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGGGTAAAGTGTTACT‑GAAGGCGGCGCCTGAGACCCCAAGGGG‑GATGCACGAACAACTG‑CGGGagatctcct > 1:462914/1‑220 (MQ=255) tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGCCTCCTTTa > 2:327219/1‑227 (MQ=255) tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGG‑GATGCACGACCCAATG‑CCGGAGGCCTTCtttc > 2:3146/1‑226 (MQ=255) gCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTAc > 2:75835/1‑227 (MQ=255) aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGCCTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACgccc > 2:234553/1‑225 (MQ=255) aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGG‑GATGCCCGAACAAATG‑CCGGAGGTCTTCTTTAAGGc > 1:609099/1‑227 (MQ=255) aaggatgtgcGATTTCGATCGCAAAGAAGTGATCGTCCCGAACAAAGGGGTTGGTACCGAGCGTTTGATCAACTTGTCGTTGACTGACACTGCTACGCGTCGGGTGATCCGTATCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCAt < 1:39276/218‑1 (MQ=255) aCCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCCCCCAAGGGT‑GGTGCCCGAACCAATG‑CCGGAAGGCTTCTTTACGGAATTTGGTGc > 1:556032/1‑227 (MQ=255) ccGATTTCGAACGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCa < 2:251574/228‑1 (MQ=255) ttCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCCTCGATCCGGAAAAAGGGGGTAAATCGTTCCG‑GAAGCGGGGGCATGAGGCCCACAGGGG‑GCGGC‑CGAACCAAAGACGGGAGGTCTTCTTCACGCGATTTGGtcgcacgca > 1:32125/1‑219 (MQ=255) tgATCGAAAAGAAGTGATCAGCCCGAGCAAGGCTTTTTTTGCGGGGCGTCTGATCAACTGGTCGTTGAATGACACTACGACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGtt < 1:476011/227‑1 (MQ=255) gcagcGCAAAGAGTTGATCACCCCGAAAAAAGCGTTTTGTACCGGGCGTTTGATTAACTGGGCGTTGACTGACACCTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGTCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGtt < 1:192128/224‑1 (MQ=255) gaacGCAAAGGAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGtt < 2:462914/224‑1 (MQ=255) aTCGCAAAGAAGGGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAACGCGGCGACTGAGCACCCAAggg > 2:534719/1‑171 (MQ=255) gaaaaaaTTGATCACCCCGAAAAAAGGTTTTGGTTCCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTTAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCa < 1:126707/227‑1 (MQ=255) gaaggtatcatcCCGAACAAAGGTTTTGTTACCGGGCGTTTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAg < 1:603080/222‑1 (MQ=255) gaagTGGTCCCCCCGAACAGAGGTTTTGTTACCGGGGGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAg < 1:634367/228‑1 (MQ=255) aaggggtcctcCCCAAAAAGGCGTTTGTTTCCGGGGTTTCGATCAACTGGTCGGTGACTGACTCTACTACGCGTTTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGAGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGc < 1:356086/219‑1 (MQ=255) tgTGATTCTCCCGAAGAAAGCTTTTTTTACCGGGGCTCTGAGCAACGGGTCGTTGGCTGACACTGCTACGGAGCTGGTGATCCGGGTCAGCGTGGCCTAGGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGcg < 1:258152/227‑2 (MQ=255) agggATCATCCCGAACAAAGCGTTGGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGGGTCTGGTGATGCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCt < 2:224261/225‑1 (MQ=255) cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGGCTTCTTTACGGCATTTGGTGCCA‑GACCGTTGGCTCATGAGCTGGGGCTg > 2:188378/1‑226 (MQ=255) cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGCGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGG‑GATGCACGAACCAATG‑CCGGAAGGCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGccactg > 2:243713/1‑221 (MQ=255) ctacacaaGCTTTTGTTCCCGAGCGTCGGATCAACTGGTGGTTGACTGACCCTGCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCCCCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTCACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTa < 1:80146/220‑1 (MQ=255) gAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGCATGAGCACCCAAGGGG‑GATGCACGAACCAATG‑CCGGAAGGCTTCTTTCCGGCATTTGGTGCCACGCA‑GTGGGATAATGAACTGCGTGTGTAtgt > 1:276863/1‑228 (MQ=255) aaGGGTTTGTTACCGGGCGTCTGTTCAACTGGTCGTTGACGGACACTACGACGCGTCTGGTGATCCGTCGCGGGGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCCGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg < 2:283333/228‑1 (MQ=255) aaGCTTTTGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg < 1:467551/228‑1 (MQ=255) aaGCGTTTGTTACCGGGCGTCTGAGCAACGGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg < 2:504409/228‑1 (MQ=255) aGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAATTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGG‑GATGCACGAACCAATG‑CCGGAAGGCTTCTTTACGGCATTTGGTGCCA‑GCCCGTTTGACAATGAGcgtgctccgtattggcgtga > 1:214629/1‑209 (MQ=255) aGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCCGCGACTGAGCACCCACGGGT‑GAAGCACGAACCAATG‑CCGGAAGCCTTCTTTACGGCCTTTGGTGCCA‑CCCCGTTGGAACATCAgcggcggtcttgggtgcgtgg > 1:602693/1‑213 (MQ=255) ttGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCCCCCAAGGGT‑GATGCACGAACCAATT‑CCGGAAGCCGTCTTTAGCGCATTTGGTGCCA‑GCCCGGTGGTACATTAGGCGCGCCGTTATGTGCGTGAAGTGc > 1:151768/1‑228 (MQ=255) gTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt < 2:76490/228‑1 (MQ=255) aGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGTACTGCGTGCCCgga > 2:513924/1‑226 (MQ=255) cGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCATATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGG‑GATCGACGAACCAATG‑TCGGAAGCCTTCTTTACGGCATTCGGGGCCC‑GCACGTTGGCTCATGAGCTCAGTCGGCATTGGCGGTAACTGCGTGAcaggaag > 1:187595/1‑222 (MQ=255) gATCAACTGGGTGTTGACTGACACTACTCCGCGTCTGGTGATCCGTCTCGGCGTGGACTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATGTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTag < 2:572909/228‑2 (MQ=255) ctgtgcGTTGCCTGACAATAATAAGCGTCTGGTGGTCCGGCCCGGCGTGGCCTATGGCTACGATCTGGAAAAAGTGCGAAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGAATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg < 2:106202/222‑1 (MQ=255) cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAAATGCGTGACCGTAGTCGTACTGTCCGTGAGc > 2:281974/1‑228 (MQ=255) tgagtggcacacttactgtgcTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGTCGGCGACTGAGCCCCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTGGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg < 2:550712/207‑1 (MQ=255) gacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGa > 2:358779/1‑125 (MQ=255) acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGaa < 1:358779/125‑1 (MQ=255) acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGCCTTCTTTACGGCATTTGGGGCCA‑GCAGTTTGGATCATGAACTGGGTCTTTATGGCGGTGAACTGCGTGACGGGAGTCGTCGTGTCgtttggcggacccgga > 1:495167/1‑213 (MQ=255) cattactccGCGTCTGGTGATCCGTCTGGGCGGGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAc < 1:234553/225‑1 (MQ=255) gtactacGCGTCTGGGGATCCGTCTCGGCGTGGCATATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTa < 2:187595/227‑1 (MQ=255) ctcggcgTTTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGGTCTGGAAAAAGGGCGGAAAGTGTTACG‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg < 1:252363/224‑1 (MQ=255) ctacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTg > 2:118544/1‑138 (MQ=255) ctacGCGTCTGGTGATCCGTCGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGTACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg < 2:32125/228‑1 (MQ=255) tacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTgg < 1:118544/138‑1 (MQ=255) ccGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGGGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTATCGATAACATGTGCCGTGa > 2:472512/1‑228 (MQ=255) gactcGGCGGTGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAAGG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaa < 1:75835/226‑1 (MQ=255) ttgcgggggggCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGGTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa < 2:609099/219‑1 (MQ=255) tcgcggcggGGCCTATGGGTGCGATCGGGAAAAAGTTCGTAAAGTGGTACT‑GAAGGCGGCGTCTGAGCACGCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCGTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGGGaaa < 2:87456/224‑1 (MQ=255) tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGGGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGGGACCGTAGTCGTACTGTGGATGAGCTGAACCGTa > 2:52881/1‑201 (MQ=255) gcgtgCCTAGGGCTCCGAACTGGAAAAAGGTGGTAAAGGGTTACT‑GAAGGGGGCGACTGGGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACAt < 2:571952/224‑1 (MQ=255) gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCaa > 2:120392/1‑227 (MQ=255) ctaatgCTCCGATCTTGAAAAAGTGCTTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGAATTTGGTGCCT‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt < 2:276863/223‑1 (MQ=255) cGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa > 2:435790/1‑228 (MQ=255) cGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa > 2:219019/1‑228 (MQ=255) cGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACAATCGTTCAGCTGTGCCGTGAAACCGCCATCACCATTGCCTTTaa > 2:644917/1‑228 (MQ=255) cGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGAACTATCGATCAGCTTTCCCTGGAAAACGAGATCACAATTGCCTTTaa > 2:371195/1‑228 (MQ=255) gATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg < 1:657509/171‑1 (MQ=255) ggAAAAAGTGGCTAAAGGTTTACTGGAGGGCGGAATCTGA‑CCGCAAAGGGTGGATGC‑CGGAGCAATT‑CCGGAAGTCTGCTTTACGGGATTTGGTGCCA‑GGACGTTGGATCATGAGCTGCGTCTGTATGTGCGGGAACTGCGTGACCGTGGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCt < 1:132936/228‑1 (MQ=255) | TATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACT‑GAAGGCGGCGACTGAGCACCCAAGGGT‑GATGCACGAACCAATG‑CCGGAAGTCTTCTTTACGGCATTTGGTGCCA‑GCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCT > NC_000913/489376‑489807 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |