| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 37,284 | T→G | 15.0% | T181P (ACC→CCC) | caiC ← | putative crotonobetaine/carnitine‑CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 37,284 | 0 | T | G | 15.0% | 122.3 / 2.1 | 68 | T181P (ACC→CCC) | caiC | putative crotonobetaine/carnitine‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base T (16/37); new base G (13/2); total (29/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCC > NC_000913/37051‑37498 | tACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTAAACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACCCGGCCAGGCGGAGTAATATCCAGCGCAGCGCAGGATGTCATGGGTAAT‑CACCCCACCATCCGGTCGGGCGGTGGTGCCGGGGGGGAagg > 2:499463/1‑238 (MQ=255) ccTGTCCCCAGAAGGCGCGGGCGCTGTCTTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCAACGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCCGGTAGACGTCATCCTCCCGCAGTGCACACTGCCAGACCGAGTAATATCCATCGCACCGCAGGTTGTAATGGGTAAT‑CACCAAACCTTTCTGGCGGGAGGTGGGGCGGGGGGGGAAggga > 2:481078/1‑239 (MQ=255) ccTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACCCCTTTCGGTCGGCAGGGGGGGCCGGGGGTGAAgaga > 1:63220/1‑239 (MQ=255) ccTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGGGGTGGTGCCGGAGGGGAAgaga > 1:212264/1‑239 (MQ=255) ccTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGGGAAgaga > 1:358393/1‑239 (MQ=255) ccTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGGAAT‑CACACCACCTTTCGGTCGGGAGGGGGGGCGGGGGGGGaaaaaa > 2:254437/1‑235 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGGGCCGGCGGTGAAGAGAAtt > 1:55759/1‑239 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACAGCCAGGCGGAGTAATATCCAGCGAAGCGCCGGGTGTAATGGGAAAT‑CACCACACCATTCGGTCGGGAGGTGGGGCCGGAGGGGAAGAAAAtt > 2:185626/1‑239 (MQ=255) gTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGGAGACGTCCTCTTCACGCAGTGCACACTGCCAGACGGCGGAATATCCACCGAAGCGCCGGTTGTAATGGGTAAT‑AACCACCACCTTCGGTCGGGAGGGGGGGCCGGAGGGGAAAGGAAtt > 2:115046/1‑239 (MQ=255) tCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGAACACTGCCAGGCGGAGTAATATCCCGCGAGGCGCAGGTTGTAATGGGTAAT‑CACCAAACCTTTCGGTCGGGAGGTGGGGCCGGGGGGGGagaggaat > 2:826409/1‑234 (MQ=255) aaGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCCGGTTGTAATGGGGAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGt > 1:532869/1‑239 (MQ=255) gcgcGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GCTATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGGCACGCAGTGCACACTGCCAGGCGGGGGAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAG‑AACCACACCTTTCGGTCGGGCGGTGGGGCCGGAGGGGGAAAGAATTTcacccggctc > 2:96052/1‑230 (MQ=255) gcgcGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGGGAAGAGAATTTCCGCCGTATc > 1:314014/1‑239 (MQ=255) cgcgGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCCGGGTGGAATGGGTAAT‑CACCCCACCCTTAGGTCGGGAGGGGGGGCCGGGGGGGAAGAGAATATCCCCCGTATCg > 2:795337/1‑239 (MQ=255) gggCGCTGTATTTCGCGACCAGCACAAAGGTGGCCCCGGCAGAAAACCCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGGCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCa < 1:51563/239‑1 (MQ=255) tttttCCCGCCCAGCAAGAAGGGGCCCCCGGCAAAAAACCCCC‑CCACCGC‑GGCAGTACCCTGGCAACC‑GATATGAACCGCAGGCATTCCCATCAGGTAGCCGTCATCGTCCCGCAGGGCACACGGCCAGGCGGAGTAATATCCAGCGAAGCGCAGTTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAg < 2:575293/237‑1 (MQ=255) aTTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGAAATC‑GATATGAACCGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAg < 1:790995/238‑1 (MQ=255) ctcGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTccatgggaaagggcg > 2:790995/1‑225 (MQ=255) ccacgccgacaaaaGGGGGCCCCGGGAAAAAACCCCC‑CCCCTGCGCGGAGGACCATGGCAAGCGGATA‑GAAACCGAGGAATTCCCGGAGGGAAGACGTCCTCGTCACGCAGTGCCCCCTGCAAGGCGGAGTAATACCCAGCGAAGCGGAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGGCCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAgcggcg < 2:180724/228‑1 (MQ=255) ccaccaCAAAGGTACCCCGGGAAAAAAACCCCG‑CCATCCC‑CGCAGTACCCTGGCAATC‑GAAATGAAACGAAGGCATTACCGTAAGGTAGCCGTCAGCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 2:817500/239‑1 (MQ=255) ccAGCAAAAGGGTGGCCCCGGAAAAAACCGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 1:866592/239‑1 (MQ=255) caagaacaGAGGTGGCCCCGGCAAAAAACGGCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCa < 1:795337/234‑1 (MQ=255) gCAAAATGGTGGCCCCGGCAGAAACCGCCG‑CCATCGC‑CGCAGTACACTGGCAACC‑GATATGAACCGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAg < 1:390756/239‑1 (MQ=255) cccaacaGGTCGCCCCGG‑AAAAAACCCCGCCCAGCGC‑GCCGGTCCAGGGGAAATC‑GATATGAAACGCAGGCATTCCGGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAg < 2:202946/233‑1 (MQ=255) cacaAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGGCAGTCGATAGCGGCGGTGGATAGc > 1:56969/1‑239 (MQ=255) cacaAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTGGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTAACGTCAGTCGATACcggcgggccgaag > 1:747298/1‑231 (MQ=255) caAAGGGTGCCCCGGCAGAAAACGCCC‑CCATGCC‑CGCAGTACCCTGGCAATC‑GATATGAAACGCAGGCATTCCCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGcac < 1:760655/239‑1 (MQ=255) ggCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGAAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGGGCCGGGGGGGAAGAGAATTTCCGCCGTTACGGCCGTCGAAAGgggggggggggaacccaacaggggggggg > 2:802276/1‑216 (MQ=255) cAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGGGAAGGGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATTGCCAAAGgggggggggggt > 1:257119/1‑231 (MQ=255) tGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATCGCGGCGGTGCATCGCCCAAGGAGGCGGGATGTTGATTTTTCCCGTGA‑GGAAAcacaccaa > 1:910764/1‑231 (MQ=255) aaTC‑GATATGAACCGCAGGCTTTCCCGGCAGGTAGACGCCATCGTCACGCAGTGCACACGGCCAGGGGGAGTAATATCCAGCGAGGCGCAGGTTGTAAGGGGTAAT‑CACCACCCCTTCCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGcc < 2:906441/238‑1 (MQ=255) aTC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGCAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGAGAGGCGCGGTGCAGATGACAAAGGGGCCGCGTGTGTGTGTTTTAGTTGA‑GAAAACGaaaccacacccc > 2:367196/1‑229 (MQ=255) c‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GGAAAAGAACTcctgcccc > 1:464642/1‑230 (MQ=255) tatGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCCTAGCACAAGGTGGGCGGTTGTTGCTTTTTCAGTTGA‑GGTACAGAACTCACGCCCtcacca > 1:102518/1‑239 (MQ=255) aaCCCAGGCATTTCCGGCAGGTAGCCGTCACCGTCCCGCAGTGCACACTGCCAGGCGGAGTAATATCCAGGGAAGCGCAGGTTGTAATGGGTAAT‑CCCCACCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCggg < 1:488526/239‑1 (MQ=255) ccGCAGGCATTACCGTCAGGTAGACGTCATCGTCCCGCAGTGCACACTGCCAGGCGGAGTAATACCCAGCGAAGGGCAGGTTGTAATTGGTAAT‑CCCCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGa < 1:185626/238‑1 (MQ=255) aGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTGGTTGATTTTTCAGTTGG‑GTAAAAGAACTCACCCCATACTCACCGGGAAgg > 1:669604/1‑237 (MQ=255) tcttcttcccggtgttcacctgcccAGGCGGTGTAATACCCGGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACCCCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTCGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAAtgtg < 2:358393/216‑1 (MQ=255) gctgcccggaGTGCCAACTGCCGGGGGGAGTAATTTCCAGGGAGGGGGAGGTTGTAATGGGTAAC‑CACCCCTCCTTTCGGTCGGGAGGGGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCg < 2:63220/230‑1 (MQ=255) tGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCGGGTTTGTGTGTTTTCAAGTGATGTAACACAAC‑CCCCCCAACCTCACCGGGAAGTGGCCCCACTGTC‑AGGaaaactgtcccccattgaaggg > 2:768410/1‑221 (MQ=255) acTGCCAGGGGGATTAATTCCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCt < 1:510359/239‑1 (MQ=255) tGCGAGGGGGAGTAATATCCGGGGGAGGGCGGGTTGTAAGGGGTAAC‑CCCCCCCCCTTTCGGGCGGGGGGGGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTc < 2:140164/239‑1 (MQ=255) gAGTATTATCCGGCGAGGGGCGGGTGGTAAGGGTTAAT‑CACCCCCCCGTTCGGGGGGGGGGGGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGGCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGaa < 2:669604/238‑1 (MQ=255) aGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAAGTGGCCGGTGTTTGATTTTTTCGTTGA‑GTAAACGAACCCACGCCATCATCAGCGGGAAGTGCCCCACCTGTCAAGGCAAAT‑TGCCGCCAATTG‑GTGGGAGCTTccgcctggaaa > 2:195219/1‑230 (MQ=255) gtatgatCCAGCAAAGGCCAGGTTTGAATGGGTAGT‑CACCAAACCTTTGGGTCGGGAGGTGGTGCCGGAGTTGAAGAGAATTTACGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGTAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATc < 1:717923/233‑1 (MQ=255) gTAATATCCAGCGAGGCGCAGGTTGTAAGGGGTAAC‑CACCACCCCTTTCGGTCGGGAGGTGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATc < 1:826409/238‑1 (MQ=255) gTAATACCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATc < 1:254437/238‑1 (MQ=255) tatgccccggggggggcgggTTGT‑ATGGGGAATCCCCCCCACCTTTGGGGTGGGGGGTGTGGCGGGAGGGGAAGAGAATTTCCGCCGTATCGTCAGTTGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGt < 2:108907/221‑1 (MQ=255) aattgccggggAAGGGCAGGTTTTAATGGGTAAT‑CACCACCCCTTTCGGGCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTg < 1:662126/228‑1 (MQ=255) tacccggcgagggtcAGGTTTGAAGGGGTAAT‑GCCCCCCCCTGTCGGTGGGGGGGGGGTGCGGGAGGGGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGTGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTg < 2:55759/225‑1 (MQ=255) tgCGAAGCTGAGTTTGTAATGGGTAAT‑CACCACACCTTTCGTTGGGGAGGTGGTGCCGGAGGTGAAGAGAATGTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:802699/238‑1 (MQ=255) ggggAAGCGGAGGTTGTAAGGGGAAAC‑CCCCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:876383/236‑1 (MQ=255) ggCGAAGCGCAGGTTGTAATGGGTAAG‑CCCCCCCCCTTCCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:499463/238‑1 (MQ=255) cgCGATGCGAAGGTTGTAATGGGTAAT‑CCCCTCTGCTTGCGGTCGGGAGGTGTTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:479318/238‑1 (MQ=255) gaaggggggtttGTAAGGGGTAAT‑CACCACTCCTTTGGGTCGGGGGGTGGTGGCGGAGGTGAAGAAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 2:257119/226‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:713925/236‑1 (MQ=255) gAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTGTGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:738444/236‑1 (MQ=255) agaGCCCAGGTTGTAAGGGGTAAT‑CACCACACCTTTCGGTCGGGAGGGGGGGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACa < 1:638/234‑1 (MQ=255) cgcAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGCTAGCGGCGGGGCATAACCCAAGGGGGCGGGTTGTTGATTTTTTAGTTGA‑GTAAACGAACTCCACCCATCACCAACGGGAAGATGCACACCTTTC‑AGGCAAATATGCCC‑CCATTGAGGGGGCTATTCCTCCTCaaaatttttgtacataagaaa > 2:738444/1‑220 (MQ=255) ggTTGGAATGGGTAAC‑GCCCACACCTTTCGGTTGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGaa < 1:92925/238‑1 (MQ=255) aaggggaaacccccccacCTTTGGGTCGGGAGGTGGTGCCGGAGGTGAAGGGAATTTCCGCCGTATGGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGCGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTgcgcg < 1:717039/224‑2 (MQ=255) aTGGTTACT‑CCCCACCCTTTTGGGGCGGGGGGGGTTGCCGGGGGTGAAGAGAATTTCCGCCTTATCTGCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGAGTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCAc < 2:102518/239‑1 (MQ=255) tattcccccctcttttGGGGCGGGGGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGt < 2:314014/225‑1 (MQ=255) t‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGGGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCC‑CAATTTAGTGGCATCTTCCTGCTGAAACTGTTTGTACATAGGGTAGAATTGCGCCCTGGGCAcc > 1:245982/1‑239 (MQ=255) cgccTTTCGGGCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGGCGATAGCGGCGGTGCATAGCCCAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAgg < 1:481078/237‑1 (MQ=255) cccttttgggggggggggggggGCGGGAGGTAGAGGAAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCCCATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAgg < 2:775960/227‑1 (MQ=255) tcggtcggGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGGTTTTTCAGTTGA‑GTAAACGAACTCACGCCCTCATCAGCGGGAAGTGCCCCATCTGTC‑AGGCAAAATTGCCC‑CCATTGAGTGGGATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTTCGCACTGGTCAccctgtctctttta > 1:644321/1‑227 (MQ=255) gCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTaaa < 1:65947/93‑1 (MQ=255) | TACCTGTCCCCAGAAGGCGCGGGCGCTGTATTTCTCGACCAGCACAAAGGTGGCCCCGGCAGAAAACGCCG‑CCATCGC‑CGCAGTACACTGGCAATC‑GATATGAAACGCAGGCATTACCGTCAGGTAGACGTCATCGTCACGCAGTGCACACTGCCAGGCGGAGTAATATCCAGCGAAGCGCAGGTTGTAATGGGTAAT‑CACCACACCTTTCGGTCGGGAGGTGGTGCCGGAGGTGAAGAGAATTTCCGCCGTATCGTCAGTCGATAGCGGCGGTGCATAGCACAAGGTGGCAGGTTGTTGATTTTTCAGTTGA‑GTAAACGAACTCACGCCATCATCAGCGGGAAGTGCCACATCTGTC‑AGGCAAATGTGCCG‑CAATTGAGTGGCATCTTCCTGCTGAATCTGTTGATACATAGGATAGAATTGCGCACTGGTCACCAGCAGGCACGCC > NC_000913/37051‑37498 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |