Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,219,538 C→T 10.6% A315V (GCG→GTG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,219,5380CT10.6% 181.3 / 9.8 73A315V (GCG→GTG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (33/29);  new base T (4/3);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

CCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTT  >  NC_000913/4219307‑4219776
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
ctgcgcgatgtcgccccgccgAAGCGGGCATTGTTTTTGGCGTTGGGCGTTCTTTTTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGt                                                                                                                                                                                                                                         <  2:700998/224‑1 (MQ=255)
caggcctgACGACGCCCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCggg                                                                                                                                                                                                                                          <  1:236745/235‑1 (MQ=255)
  ttcctgtgtaccgccgccgAAGCGGGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTAGGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAACCCACCGCTGAATTGTATATAGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTAt                                                                                                                                                                                                                                       <  2:270079/226‑1 (MQ=255)
            cgcccgccgAAGCGATCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAAt                                                                                                                                                                                                                              <  1:481870/235‑1 (MQ=255)
              acCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACCCCAAAACCGAAAGCTACCGCGGATATCTCGTTTTTTTACAGGGGCGTAATGAGGAGTTCATTGAAAGACCGGGTATTTGGGGaacag                                                                                                                                                                                                                            >  2:236745/1‑235 (MQ=255)
                    gAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCGACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGtt                                                                                                                                                                                                                     <  1:211822/239‑1 (MQ=255)
                           gCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGtt                                                                                                                                                                                                               <  1:595514/238‑1 (MQ=255)
                             aTTTTTTTCGGATTTGCGCGTTCTTATTTTATGGTGTATGCGCCGCTGCCCGGATCGCTGGTCGAGTGGCTCCGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTa                                                                                                                                                                                                             <  1:663049/238‑1 (MQ=255)
                                  ttttGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGGCGGTGCCCGCAGCGCTGGTCGGGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGc                                                                                                                                                                                                       <  1:446636/239‑1 (MQ=255)
                                  ttttGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGc                                                                                                                                                                                                       <  1:723018/239‑1 (MQ=255)
                                           ttaagatgccgaTTTTTTGGTTTTGGCGCCGCTGCCCGCACGCCTGGGCGGGGGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                              <  2:732345/228‑1 (MQ=255)
                                             cgcgTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGACAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGAt                                                                                                                                                                                            <  1:138849/239‑1 (MQ=255)
                                                 ttCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCCCGTTTATCTACAGGGCCGTAATGAGCAGTTCATTGAAGCGCCGGGTATTTGTGGAATGGGGGTGTTGGGGTTTACCCTGCCGGGCTTTGATCggg                                                                                                                                                                                        >  2:755997/1‑239 (MQ=255)
                                                          tATGGTTTAGGCGCCGCTGCCCGCAGGGCTGGTCGAGTGGCGTCGGGAAATTCTGCCAGGTAAAACCAACGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAg                                                                                                                                                                               <  1:325518/239‑1 (MQ=255)
                                                            tGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGGGTTGTTGGTGTTTACGCTGCCGGGGTTTGTACGGGGTTTCAAAgac                                                                                                                                                                             >  2:366257/1‑237 (MQ=255)
                                                            tGGTTTATACGCCGCTGCCAGCAGCGCTGGTCGAGTGGCTATGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCAAACACCGAAAGCTACCGCGAGAATCTCGTTTATCTACAGGGCTGTAATGAGACGTTCATTGAACACCCGGGTCTTCGTGCAATGGGGATGTTGGGGGTTACACCGCGGGGCTTTAGTCgggggttaaagaga                                                                                                                                                                             >  2:172597/1‑228 (MQ=255)
                                                              gTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCCGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGGATTCaaataact                                                                                                                                                                           >  2:145732/1‑234 (MQ=255)
                                                                 tATGCGCCGCTGCCCGCAGCGTTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAACAACACCGCTGAATTGTATATGTCTATCGGCTGCCAGAAGCACGCCAAAACCGCAAGCTACCGCGAATATCTCGTTCATCTCCAGGGCTGTAATGAGAAGTTCATTTAACCCCCGGGCCATTGTGGAAGGTGGACGTTGGAGTTTACGCGCCCGGGCTTTGACCGGGGATGCaaaatggaaac                                                                                                                                                                        >  2:731111/1‑231 (MQ=255)
                                                                 tATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGGATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGAAtt                                                                                                                                                                                    >  1:732345/1‑227 (MQ=255)
                                                                 tATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                              >  1:458458/1‑217 (MQ=255)
                                                                 tATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTg                                                                                                                                                                                              <  2:458458/217‑1 (MQ=255)
                                                                    gcgcCGCTGCCCGCAGGGCTGGTCGGGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGa                                                                                                                                                                     <  1:811380/239‑1 (MQ=255)
                                                                                         gTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCCGTAATGAGCAGTTCATTGAAGCGCCGGGGATTCGTGGAATGGGGATGTTGGTGTTTACGCTGCCGGGCGTTGATCGGGAATTTCAAGTAACCAAAAAACGGTTGGCGCCCCGGaagag                                                                                                                                                >  2:103916/1‑236 (MQ=255)
                                                                                          tCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATAAAAGACAGGTTCGCGCCCCAGAAaggg                                                                                                                                               >  1:152132/1‑237 (MQ=255)
                                                                                              ggggCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGGCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGAt                                                                                                                                                                                            <  2:342722/188‑1 (MQ=255)
                                                                                              gTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGAt                                                                                                                                                                                            >  1:342722/1‑190 (MQ=255)
                                                                                                                 ccAGGAAAACCCACCGCTGAATTGTATATGGGTAACGGATGCCAGAAGCGCGCCAAAGCCGAAAGCTACGGCGAATATCTGGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGGGTCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                        <  1:116784/239‑1 (MQ=255)
                                                                                                                  cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTg                                                                                                                         >  1:96110/1‑237 (MQ=255)
                                                                                                                  cAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                        <  1:755997/238‑1 (MQ=255)
                                                                                                                        aaacccccccttaatttgttttGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTTCCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTAt                                                                                                                 <  2:96110/218‑1 (MQ=255)
                                                                                                                              ccGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCGGGGCTTTGATCGGGTATTCAAAGTCTTCAAAGACAGGTTCCGCCCCCAGAAAGAGAAGTCTGCCGCTCCCGTTCGGGCGTGCTATAAAcgg                                                                                                           >  2:68006/1‑237 (MQ=255)
                                                                                                                               cGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGGATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGCCGTCCGCTCACGTTCGTGCCGGcgaagaacggg                                                                                                          >  2:775064/1‑229 (MQ=255)
                                                                                                                                  tgaatttttattGGCTTTCGGCTGCAGGAAGGCCGCCAAAACCGAAAGCTACCGGGAATATCCCGTTTTGCTACAGGGCTGTAATGAGCAGGTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGa                                                                                                       <  2:155217/228‑1 (MQ=255)
                                                                                                                                  tGAATTGTATATGGCTATCTGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGa                                                                                                       <  1:259471/239‑1 (MQ=255)
                                                                                                                                    aaTTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGaaa                                                                                                     >  1:627601/1‑239 (MQ=255)
                                                                                                                                         tataTGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGc                                                                                                 >  1:179214/1‑238 (MQ=255)
                                                                                                                                           tatGGCTTGCCGCTGCCAGAAGCCCCCCAAAACTGAAAACTCCCTGGAATATCTCGGTTATCTACAGGGCCGAAAGGAGCATTTCCTTGAAGCGCCGGGTGTTCGGGGAATGGTGATGTTGGTTGTTACGCTAGCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTTCCTGCTATCAACTGGTGAAAGAGCACg                                                                                              <  2:558195/239‑1 (MQ=255)
                                                                                                                                                  aTCGGCTGCCAGAAGCACGCCAAAGCCGAATGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGt                                                                                       <  1:361660/239‑1 (MQ=255)
                                                                                                                                                   tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                        >  2:189033/1‑205 (MQ=255)
                                                                                                                                                   tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                                                        <  1:189033/205‑1 (MQ=255)
                                                                                                                                                   tCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGGGCCTCCTATCACCTGGGGAAAAAGCACCATcgccgg                                                                                      >  1:611640/1‑236 (MQ=255)
                                                                                                                                                        tGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGAGATGTTGGTGTTTACGCGGCCGGGCTTTGATCGGGTATTCAAGAGCATCAAAGACAGGTTCCCGCGGCGACAAGAGATGTCTGCCGCTCACGTCTGTTCCTGCTATCAACGGGTGAAagaggaggagcgggggggagg                                                                                 >  2:789305/1‑222 (MQ=255)
                                                                                                                                                        tGCCAGAAGCACCGCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATTTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGcc                                                                                  <  1:103916/238‑1 (MQ=255)
                                                                                                                                                          ccAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                        <  1:472288/182‑1 (MQ=255)
                                                                                                                                                          ccAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                        >  2:472288/1‑182 (MQ=255)
                                                                                                                                                               aGCACGCCAAAACCGAAAGCTTCCGGGAATTTCTTGTTTATCTACAGGGCTGTAATGGGCAGGTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGc                                                                           <  1:635361/238‑1 (MQ=255)
                                                                                                                                                                  aCGCCAAACCCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGTGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCgg                                                                         <  1:366257/237‑1 (MQ=255)
                                                                                                                                                                                             atCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCgg                                                                         <  1:598941/210‑1 (MQ=255)
                                                                                                                                                                                                    ttaacgaCAGGGCTTTAATGAGCGGTTCATTGAAGCGCCGGGTATTCGTTGAATGGTGATGTTGGGGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAg                                      <  1:102279/232‑1 (MQ=255)
                                                                                                                                                                                                       tCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCCGGAGTTTGAAAACTTTTTGCTGGAGAAGCGGc                                  >  1:531387/1‑239 (MQ=255)
                                                                                                                                                                                                       tCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTCTGTGCTGGAGAAGCGGc                                  >  1:432639/1‑239 (MQ=255)
                                                                                                                                                                                                       tCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGGGAAAGAGCACGATCGCGTGGGCCGCAGTGCGGGCACCCAGGa                                                               >  2:598941/1‑210 (MQ=255)
                                                                                                                                                                                                            aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGGGAAAGTGCACGATCGCGTGGGCCCGCTGCCGGCAACCCAGGAATTTGAAAACTTTGTCGTGGAGGAGGGGGATAtt                             >  2:177317/1‑239 (MQ=255)
                                                                                                                                                                                                            aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCAATCAACTGGTGAAAGAGCACGATGCCGTGGGCCGAGTGGCCGACACCCAGGAGTTTAAAAACTTTGGCCTGGAGAAGCGGGAAAtt                             >  2:252534/1‑239 (MQ=255)
                                                                                                                                                                                                              ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                        <  2:30244/130‑1 (MQ=255)
                                                                                                                                                                                                              ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                                                        >  1:30244/1‑130 (MQ=255)
                                                                                                                                                                                                              ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGGTTGAAAACTTTGGGCTGGAGAAGCGGCAAAttt                            >  1:772479/1‑238 (MQ=255)
                                                                                                                                                                                                              ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCCCACGTTCGTGCCTGCTTTCAACTGGTGAACAGGCCAGATCCCGTGGCCCGAATGGCGGCCACCCAGGAGTTTGAAAAATTTGTCCTGGGGAAGGGGCaatttt                            >  2:795852/1‑233 (MQ=255)
                                                                                                                                                                                                                 tggaaTGATCAGTTCAGTGAAGGTCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTGGATCGGGCATTCAAAGGCAGCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCGGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTccc                         <  1:311230/235‑1 (MQ=255)
                                                                                                                                                                                                                 tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCACCTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTcccc                        >  2:25734/1‑239 (MQ=255)
                                                                                                                                                                                                                 tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGGGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCCCAGAAAGGGATGTGTGCCGCTCCCGTTCGTCCCTGCTATCCACTGGTTGAAAAGGCCATTCGCGGGGGCCGAATGGCGGACACCCAGGGGTTTTAAAAAATTGTGGGGGAAAAGGGGGAAATTTaccc                        >  2:394918/1‑239 (MQ=255)
                                                                                                                                                                                                                 tGTAATGAGCAGTTCATTGAAGCGCCGGGGATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGGGCACGAACGCGTGGGCCGCATGGCGGACACCCAAGAGTTTGAAAACTTTTGACGGGAGAAGGGGCATATTAcccc                        >  2:546819/1‑239 (MQ=255)
                                                                                                                                                                                                                   tAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCCACTGGGGAAAGAGCACGATCGCGGGGGCCGAATGCCGGACACCCAGGAGTTTGAAAACTTTTTGCTGGAGAAGCGGGATATTTCCCCgg                      >  2:449733/1‑239 (MQ=255)
                                                                                                                                                                                                                        aGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGAGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCAATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGGTTGAAAACTTTTGGCTGGGGAACGGGCAAATTTCCCCGGCATTa                 >  2:350156/1‑239 (MQ=255)
                                                                                                                                                                                                                          cAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGGGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCGGGAGAAGAGGCCAATTTCCCCGGCCTTAAt               >  1:874173/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGGCGGGTATTCGTGGAATGGTGCTGTTGGAGGTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGGCATCCAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCTTGCTATCAACTGTTGAAAGAGAACGATCGCCAGGGCCGAAAGCCTGCCACCCAGGAGGGTGAAACATTTGTGCTGAGGAAGCGGCATATATGCCCGGCAATaaagaa            >  2:115899/1‑235 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGTCACCCAGGGGTTTGAAAACTTTGGGCTGGGGAAAGGGCGAATTTACCCGGCATTAATGGa            >  2:228578/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGGGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGa            >  1:327727/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGa                                                         <  1:343091/194‑1 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGACCGCGTGGGCCGAATGGCGGACACCCAGGGGTTTGAAAACTTTGTGCGGGGGAAGGGGCAAAATTCCCCGGCTTTCAGGGa            >  2:851170/1‑239 (MQ=255)
                                                                                                                                                                                                                             ttCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCCAACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCCCGAACGCGTGGGCCGAAGGGCGGCACCCCAGGAGTTTGAAAAACTTGTGTGGGGGCAGGGGAATATTTCCCCgcgcttaaagaa            >  2:488529/1‑228 (MQ=255)
                                                                                                                                                                                                                               cATTGAAGCGGCGGGTATTCGTTGAATGGTGATGTTGGTGTGTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCg                                    <  1:907183/213‑1 (MQ=255)
                                                                                                                                                                                                                                 ttGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTa        <  1:228578/239‑1 (MQ=255)
                                                                                                                                                                                                                                       cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTAACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTTGGCTGGAGAAGCGGCAAATTTCACCGGAATTAATGAAAATactacta  >  2:223491/1‑238 (MQ=255)
                                                                                                                                                                                                                                       cgcCGGGGATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGGGCACGATCGCGGGGGCCGAATGGCGGCCACCACGGAGTTTGAAACATTTGTGCTGGGAAAGCGGGAGTTTTCCACCGCCATAAGTGAAATACTGttt  >  2:615767/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              
CCTGCCTGACGACGACCGCCGAAGCGAGCATTGTTTTTGGCTTTGCGCGTTCTTATTTTATGGTTTATGCGCCGCTGCCCGCAGCGCTGGTCGAGTGGCTACGGGAAATTCTGCCAGGTAAAACCACCGCTGAATTGTATATGGCTATCGGCTGCCAGAAGCACGCCAAAACCGAAAGCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTT  >  NC_000913/4219307‑4219776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: